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WORLD STEM CELL SUMMIT 2015 POSTER ABSTRACTS Poster Abstracts for Presentation

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WORLD STEM CELL SUMMIT 2015 POSTER ABSTRACTS Poster Abstracts for Presentation WORLD STEM CELL SUMMIT 2015 POSTER ABSTRACTS Poster Abstracts for Presentation Laurel Coote Submission ID: 120105 Submission Title: Role of Viability and TNC count in the cord blood transplantation Submission Type: Poster Presentation Review Status: Accept Author(s) Ahmed Alrabai Student College of Medicine , King Khalid University , Abha , KSA Co-Author(s) Hussian Yasser Kariri - Student, College of Medicine, King Khalid University, Abha , KSA Ahmed Al-Hakami - Assistant Professor , Department of Microbiology and Parasitology, Center fo Stem Cell research, College of Medicine, King Khalid University, Abha, KSA Mohammed Eaiaz Ahmed Shariff - Assistant Professor , Department of Physiology, College of Medicine, King Khalid University, Abha, KSA Abdullah Saeed Assiri - Professor , Department of Medicine, Division of Cardiology, College of Medicine, King Khalid University, Abha, KSA Harish C Chandramoorthy - Assistant Professor , Center for Stem Cell Research, Department of Microbiology and Parasitology, College of Medicine, King Khalid University, Abha, KSA Topic Basic Research, Medicine, and Health Problem There is much literature evidence available today to support the success of cord blood hematopoietic stem cell transplantation for malignant and non-malignant diseases. Cord blood transplantations are attempted in almost all parts of the world with increasing number of cord blood banks and transplantations occurring in third world and developing countries. In many of the publications either from developed world or developing world we see that for successful cord blood transplantation, the criteria indicated includes viability and TNC numbers, however there is no uniformity in the establishment of the range and many such studies claims even low viability and cell numbers does not hinder success of the grafting and homing of stem cells. Background It known that viability of the progenitor stem cells remains as one of the major criteria for successful transplant outcome after the cell number. It may be noted that the cell viability and total nucleated cell (TNC) count varies from country to country and among continents with a marginal reduction of the cell viability and TNC volume during long term cyrostorage storage, processing methods, transportation and air lifting to the site of the therapy. Though there are very few controlled studies which implies on the role and importance on the success of engraftment and homing of the progenitor stem cells, largely many studies do not comment or even mention the viability percentage nor try to equate or compare their failure with the viability or cell number. Hypothesis We did an Insilco search and analysis worldwide on the available literature evidence on CBT from year 2005 and above. The logic points of interest was to investigate the 1. Direct relationship between the cell viability and TNC count in the successful outcome of cord blood transplantation. 2. Whether there is difference in the pattern of outcome with malignant and non-malignant disease conditions with respect to the TNC count and viability. 3. What impact doe’s the viability alone has irrespective of TNC count or storage between malignant and non-malignant disease. 4. Whether there is a phenomenal difference between the long term cyrostored cord blood stem cells and relatively fresh in the transplant outcome. 5. Is there difference between developed and developing world on the use of the viability as vital factor for successful transplant outcome. Research Based on the hypothetical questions raised, we searched and scrutinized various literature evidences such as full research papers, case reports, follow up studies from standard sites like PUBMED, SCOPUS etc. Analysis was done for parameters like type of transplant, source of cord blood stem cells, clinical condition, number of successful transplant outcome, viability, total nucleated cell count, age factor. Observations Upon the analysis, we observed that a good number of publications had indicated viability range and almost all the publications had indicated the total nucleated count as such or CD34+ cells. The transplant outcome in many studies were more than 60% with few exceptional case reports which did not have a follow up information or abruptly ended with transplantation. We could not find much difference in the transplant outcome compared with the developed world. Even the studies with low viability did not indicate a negative transplant outcome, however in such studies; we count not find any follow up nor were such reports not repeated again. Inference and Conclusion: Our investigation revealed the growing importance of the cord blood transplantation. Non- malignant diseases interventions with CBT are more common with highest percentage of success compared to malignant diseases. Viability alone stood as important deciding factor in most of the transplantation while were saw a wide range of total nucleated cells used in the transplant outcome. Conclusions Submission ID: 132452 Submission Title: Cross-species chimerism with human naïve Induced pluripotent stem cells Submission Type: Poster Presentation Review Status: Maybe Author(s) Ohad Gafni, PhD student Weizmann Institute of Science Co-Author(s) Sergey Viukov Mirie Zerbib Rada Massarwa Jacob Hanna Topic Basic Research, Medicine, and Health Problem Mouse embryonic stem cells (mESCs) are isolated from the inner cell mass (ICM) of blastocysts, and can be preserved in vitro in a naïve ICM-like configuration by providing exogenous stimulation. While human embryonic stem cells (hESCs) share several molecular features with naïve mESCs, they also share a variety of epigenetic properties with primed murine Epiblast stem cells (mEpiSCs). While human embryonic stem cells (hESCs) share several molecular features with naïve mESCs, they also share a variety of epigenetic properties with primed murine Epiblast stem cells (mEpiSCs). Thus, prolonged culturing of human stem cells in naïve conditions remains elusive. Background Our group defined growth conditions that facilitate the derivation of naïve human induced pluripotent stem cells (hiPSCs) that retain growth characteristics, molecular circuits, chromatin landscape, and signaling pathway dependence that are highly similar to mouse naïve ESCs, and drastically distinct from conventional primed human pluripotent stem cells. Hypothesis Although human in-vitro differentiation protocols have been progressed significantly over the last few years, the need for a platform that will emulate in-vivo differentiation is still very much desired. Animal-human chimerism can be used to grow human organs and/or tissues as incubators. The potential of naïve hiPSCs that will be able to engraft in the host inner cell mass, and faithfully contribute to chimeric animals is not defined yet. Research To address this issue, we engineered naïve hiPSC knock-in line that constitutively expresses eGFP. These cells were injected into mouse E2.5 morulas, implanted into pseudo-pregnant female mice and their progeny was traced during embryonic development, using immuno-histological analysis, in toto live confocal imaging, and flow cytometry (FACS). Observations Remarkably, our conditions of naïve hiPSCs are competent in cross-species chimerism and can contribute to a variety of mouse embryonic tissues, including craniofacial tissues, neural folds, heart, and hematopoietic lineage. We have improved the protocol up to 40% chimerism. Conclusions Naïve human induced pluripotent stem cells can be maintained in culture. These cells can effectively contribute to the mouse inner cell mass, and now we focus our efforts on obtaining in depth functional and developmental analysis of the in vivo integrating naïve hiPSCs, in a variety of tissues at advanced stages of mouse development. This study paves the way for human iPSCs therapeutic application in regenerative medicine, personalized medicine, and human disease modeling. Submission ID: 135298 Submission Title: Sustained levels of FGF2 reduce differentiations of stem cell cultures with less frequent media changes Submission Type: Poster Presentation Review Status: Accept Author(s) Teresa Maietta Lab Technician StemCultures LLC Co-Author(s) Steven Lotz Philip Manos William Prics Jeffrey Stern Sally Temple Christopher Fasano Topic Basic Research, Medicine, and Health Problem Current protocols for stem cell maintenance involve frequent feeding with growth factor-containing medium. For example, standard culture methods to maintain undifferentiated pluripotent stem cell cultures require daily replacement of the culture medium, making the care of these cells costly and labor intensive. Importantly, daily medium changes greatly reduce but do not fully eliminate spontaneous differentiation of pluripotent stem cell cultures, which leads to a gradual loss of potency and, often, to premature termination of the cultures. Fibroblast growth factor 2 (FGF2 or basic FGF) is a critical medium component for maintenance of a number of stem cell types, including human pluripotent stem cells. FGF2 has been reported to be highly labile at 37C, and we confirm dramatic fluctuations in FGF2 levels in standard stem cell culture protocols. Background Stem cells possess two hallmark properties: self-renewal and the ability to differentiate into one or more mature cell lineages. Most uses of stem cells involve first a period of culture in conditions that promote self-renewal to increase the number of stem cells, then a subsequent period of culture in distinct conditions that promote
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