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Polyclonal Antibody to NOS1 / Bnos (+ Inos + Enos) - Aff - Purified

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Polyclonal Antibody to NOS1 / Bnos (+ Inos + Enos) - Aff - Purified OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] SP5118P Polyclonal Antibody to NOS1 / bNOS (+ iNOS + eNOS) - Aff - Purified Alternate names: Constitutive NOS, N-NOS, NC-NOS, NOS type I, Neuronal NOS, Nitric oxide synthase, nNOS Quantity: 0.1 ml Concentration: 0.14 mg/ml Background: Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L- arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. bNOS and eNOS share approximately 50% amino acid sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. iNOS, however, is tightly bound to calmodulin and acts independently of calcium levels. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short time. In vascular endothelial cells NOS appears to modulate vasotension and platelet aggregation. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain. Uniprot ID: Q9Z0J4 NCBI: NP_032738.1 GeneID: 18125 Host: Rabbit Immunogen: Synthetic peptide corresponding to residues 1113-1122 of murine iNOS and bNOS. AA Sequence: D Q(1113) K R Y H E D I F G(1122) Remarks: This sequence is highly conserved among the different NOS isoforms. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20160817 1 / 3 SP5118P: Polyclonal Antibody to NOS1 / bNOS (+ iNOS + eNOS) - Aff - Purified Format: State: Liquid purified IgG fraction Purification: Antigen Affinity Chromatography Buffer System: PBS Preservatives: 0.05% Sodium Azide Stabilizers: 1 mg/ml BSA Applications: Western Blot: 1/1,000. This antibody detcts an ~135 kDa protein representing Mouse macrophage iNOS, an ~155 kDa protein representing Rat, Bovine and Porcine bNOS, an ~140 kDa protein representing Porcine and Bovine eNOS. Immunohistochemistry on Frozen Sections: 1/100. Staining of NOS in Drosophila optic tissue with SP5118P results in staining of the optic nerve. In immunohistochemistry procedures the antibody also stains NOS in Human endothelial cells, macrophages, and peripheral neural tract cells. Immunofluorescence/Immunocytochemisty. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: The antibody detects - Mouse macrophage inducible nitric oxide synthase (iNOS); - Rat, Bovine, Drosophila and Porcine brain Nitric Oxide Synthase (bNOS); - Human, Porcine and Bovine epithel Nitric Oxide Synthase (eNOS). Add. Information: Published Species Reactivity: Arthropod (Ar), Crab (Cb), Cnidarian (Cn), Drosophila (Dm), Insect (Ins), Mollusca (Mc), Mouse (Ms), Rabbit (Rb), Rat (Rt), Shark (Sh). Storage: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Lee CY, Zou HS, Yau SM, Ju YR, Liau CS. Nitric oxide synthase activity and immunoreactivity in the crayfish Procambarus clarkii. Neuroreport. 2000 Apr 27;11(6):1273-6. PubMed PMID: 10817606. 2. Zayas RM, Qazi S, Morton DB, Trimmer BA. Neurons involved in nitric oxide- mediated cGMP signaling in the tobacco hornworm, Manduca sexta. J Comp Neurol. 2000 Apr 17;419(4):422-38. PubMed PMID: 10742713. 3. PNAS Vol. 102, No. 11 4010-4015, 2005 4. JPET Nov 2005 doi:10.1124/jpet.105.093542 5. Wang Y, Kodani E, Wang J, Zhang SX, Takano H, Tang XL, et al. Cardioprotection during the final stage of the late phase of ischemic preconditioning is mediated by neuronal NO synthase in concert with cyclooxygenase-2. Circ Res. 2004 Jul 9;95(1):84-91. Epub 2004 May 27. PubMed PMID: 15166094. 6. Pechenik JA, Cochrane DE, Li W, West ET, Pires A, Leppo M. Nitric oxide inhibits metamorphosis in larvae of Crepidula fornicata, the slippershell snail. Biol Bull. 2007 Oct;213(2):160-71. PubMed PMID: 17928523. 7. Krönström J, Dupont S, Mallefet J, Thorndyke M, Holmgren S. Serotonin and nitric oxide interaction in the control of bioluminescence in northern krill, Meganyctiphanes norvegica (M. Sars). J Exp Biol. 2007 Sep;210(Pt 18):3179-87. PubMed PMID: 17766295. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20160817 2 / 3 SP5118P: Polyclonal Antibody to NOS1 / bNOS (+ iNOS + eNOS) - Aff - Purified 8. Am J Physiol Cell Physiol Vol 285, 1207-1218, Nov 2003 9. JBC Vol 279, No 51, 53475-53482, Dec 2004 10. Am J Physiol Cell Physiol Vol 285, 1207-1218, Jul 2004 11. Mahadevan A, Lappé J, Rhyne RT, Cruz-Bermúdez ND, Marder E, Goy MF. Nitric oxide inhibits the rate and strength of cardiac contractions in the lobster Homarus americanus by acting on the cardiac ganglion. J Neurosci. 2004 Mar 17;24(11):2813-24. PubMed PMID: 15028775. Pictures: Immunolocalization of NOS in Drosophila optic nerve using antibody SP5118P For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20160817 3 / 3 Powered by TCPDF (www.tcpdf.org).
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