RESEARCH ARTICLE Dop1R1, a type 1 dopaminergic receptor expressed in Mushroom Bodies, modulates Drosophila larval locomotion 1,2 1,3 1,4 Bryon Silva , Sergio HidalgoID , Jorge M. CampusanoID * 1 Laboratorio NeurogeneÂtica de la Conducta, Departamento de BiologõÂa Celular y Molecular, Facultad de Ciencias BioloÂgicas, Pontificia Universidad CatoÂlica de Chile, Santiago, Chile, 2 Genes and Dynamics of Memory Systems, Brain Plasticity Unit, CNRS, ESPCI Paris, PSL Research University, Paris, France, a1111111111 3 School of Physiology, Pharmacology and Neuroscience, Faculty of Life Science, University of Bristol, a1111111111 Bristol, United Kingdom, 4 Centro Interdisciplinario de Neurociencias, Pontificia Universidad CatoÂlica de a1111111111 Chile, Santiago, Chile a1111111111 *
[email protected] a1111111111 Abstract OPEN ACCESS As in vertebrates, dopaminergic neural systems are key regulators of motor programs in insects, including the fly Drosophila melanogaster. Dopaminergic systems innervate the Citation: Silva B, Hidalgo S, Campusano JM (2020) Dop1R1, a type 1 dopaminergic receptor Mushroom Bodies (MB), an important association area in the insect brain primarily associ- expressed in Mushroom Bodies, modulates ated to olfactory learning and memory, but that has been also implicated with the execution Drosophila larval locomotion. PLoS ONE 15(2): of motor programs. The main objectives of this work is to assess the idea that dopaminergic e0229671. https://doi.org/10.1371/journal. systems contribute to the execution of motor programs in Drosophila larvae, and then, to pone.0229671 evaluate the contribution of specific dopaminergic receptors expressed in MB to these pro- Editor: Efthimios M. C. Skoulakis, Biomedical grams. Our results show that animals bearing a mutation in the dopamine transporter show Sciences Research Center Alexander Fleming, GREECE reduced locomotion, while mutants for the dopaminergic biosynthetic enzymes or the dopa- mine receptor Dop1R1 exhibit increased locomotion.