For Research Use Only

Ninein Monoclonal ANTIBODY www.ptglab.com Catalog Number:67132-1-Ig

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 67132-1-Ig BC034708 G purification Size: GeneID (NCBI): CloneNo.: 150UL , Concentration: 1000 μg/ml by 51199 1B10A8 Bradford method using BSA as the Full Name: Recommended Dilutions: standard; (GSK3B interacting protein) WB 1:2000-1:10000 Source: Calculated MW: IHC 1:250-1:1000 Mouse 2090 aa, 243 kDa IF 1:250-1:1000 Isotype: Observed MW: IgG1 220 kDa Immunogen Catalog Number: AG14652

Applications Tested Applications: Positive Controls: IF, IHC, WB, ELISA WB : HeLa cells, MCF-7 cells, NCCIT cells, HT-29 cells Species Specificity: IHC : mouse brain tissue, mouse heart tissue Human, Dog Note-IHC: suggested antigen retrieval with IF : MDCK cells, TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother and the Background Information minus-ends of both . Ninein interacts with γ -tubulin and nucleation and anchoring at the .

Storage: Storage Store at -20°C. Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

Various lysates were subjected to SDS PAGE Immunofluorescent analysis of (-20°C Ethanol) Immunohistochemical analysis of paraffin- followed by western blot with 67132-1-Ig (Ninein fixed MDCK cells using 67132-1-Ig (Ninein embedded mouse brain tissue slide using 67132-1- antibody) at dilution of 1:5000 incubated at room antibody) at dilution of 1:500 and CoraLite488- Ig (Ninein antibody) at dilution of 1:500 (under 10x temperature for 1.5 hours. Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). lens). Heat mediated antigen retrieval with Tris- EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin- embedded mouse brain tissue slide using 67132-1- Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris- EDTA buffer (pH 9.0).