LEGENDScreen™ Human and Mouse PE Kits

Individual fluorophore-conjugated antibodies or antibody cocktails have been used as powerful tools for immunology, cancer, and stem cell research. However, there is an increasing need for tools that can quickly screen cells for a large number of cell surface markers. The BioLegend LEGENDScreen™ Human and Mouse PE Kits are made to meet this need. Why choose LEGENDScreen™? • Large selection of specificities per kit. The Human PE Kit has 361 cell surface markers plus 10 isotype controls. The Mouse PE Kit has 255 cell surface marker antibodies plus 11 isotype controls. • Fast and easy-to-follow protocol. • Directly conjugated antibodies at pre-titrated optimal concentrations provide reliable results. • Complete kits with staining buffer, fixation buffer, and plate BioLegend's LEGENDScreen™ products are lyophilized, fluorophore-conjugated antibodies provided in 96-well plates for sealers. the purpose of screening cell surface molecules on your cells of interest. Introducing LEGENDScreen™ Human and Mouse PE Kits Product Size Cat. No. LEGENDScreen™ is the most cost-effective way to test this manydirectly- conjugated antibodies in a single assay. LEGENDScreen™ Human PE Kit 1 kit (1 test per antibody) 700007 These kits can be used to screen cell lines and primary cells (PBMCs, bone marrow-derived cells, and cells isolated from tissues such as spleen and lymph node). The workflow is simple and easy. Just reconstitute LEGENDScreen™ Mouse PE Kit 1 kit (1 test per antibody) 700005 the antibodies, add your cells, collect the samples, and then analyze. Cell staining is done directly in the plates, so no additional transfer of reconstituted antibody is required. Learn more about LEGENDScreen™ at: The LEGENDScreen™ Human and Mouse Cell PE Kits are convenient, biolegend.com/legendscreen easy-to-use, and powerful tools for immunology, stem cell, cancer research, and several other fields of research. • Plate Maps • Antibody Specificities and Clones Customize LEGENDScreen™ • Protocols We understand your needs for customization of research tools to fit • FAQs your specific requirements. Our Custom Solutions Team can customize • Performance Data LEGENDScreen™ products to include only the specificities that you want and provide them in bulk sizing. Learn more about custom products at: biolegend.com/custom_solution BioLegend is ISO 13485:2003 Certified

Toll-Free Tel: (US & Canada): 1.877.BIOLEGEND (246.5343) Tel: 858.768.5800 biolegend.com

07-0054-03

World-Class Quality | Superior Customer Support | Outstanding Value LEGENDScreen™ Human and Mouse PE Kits Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo Specificity Hu Mo 33D1 • CD45RA • CD98 • CD165 • CD255 • • CD370 • • Ksp37 • TCR α/β • 4-1BB Ligand • • CD45RB • • CD99 • CD258 • CD166 • CD371 • • LAP • • TCR β chain • APCDD1 • CD45RO • CD100 • • CD169 • • CD261 • LOX-1 CLEC4A • • TCR γ/δ • • B7-H4 • • CD45R/B220 • CD102 • CD170 • CD262 • • CX3CR1 • • LPAM-1 • TER-119 • C3aR • CD45.1 • CD104 • • CD172a CD263 • • CXCL16 • LT β R • TIGIT • Cadherin 11 • CD45.2 • CD105 • • (SIRPα) CD265 • CXCR7 • LY6G6D • TIGIT (VSTM3) • CCRL2 • CD46 • CD106 • • CD172a/b CD266 • (SIRPα/β) • DcTRAIL-R1 Ly-6A/E • Tim-2 CCX-CKR CD47 CD107a CD267 • • • • • • • • Ly-6C (CCRL1) • CD172g DcTRAIL-R2 • • Tim-4 • • CD48 • • CD107b • • CD268 • • CD1a (SIRPγ) Delta Opioid Ly-6D • TLR4 (CD284)/ • CD49a CD109 CD269 • • • • • MD2 Complex • CD1b CD178 • • Receptor Ly-6G • • CD49b • • CD111 • CD271 • CD179a • • DLL1 • • Ly-6G/Ly-6C • TLT-2 • • CD1c • CD49c CD272 • CD112 • • • DLL4 TM4SF20 CD1d CD179b • • • Ly-6K • • • • CD49d • • CD114 • CD273 • CD180 • • Dopamine Ly-49A • TMEM8A • CD2 • • CD49e CD274 • • CD115 • • • • Receptor D1 • TNAP CD3 CD181 • Ly-49C/F/I/H • • • • CD49f • CD116 • CD275 • • (DRD1) CD3ε • CD182 • • Ly49D • TRA-1-60-R • CD50 • CD117 • • CD276 • • DR3 • • CD4 • • CD183 • • Ly49H • TRA-1-81 • CD51 • • CD119 • CD277 • E-Cadherin • CD5 • • CD184 • • Ly-51 • TRA-2-49 • CD52 • CD120a • CD278 • • EGFR • CD6 • • CD185 • • Ly108 • TRA-2-54 • CD54 • • CD120b • • CD279 • • EphA2 • CD7 • CD186 • • Lymphotoxin Trem-like 4 CD55 • • CD121a • CD282 • erbB3 • • • CD191 β Receptor CD8a • • CD56 (NCAM) • CD284 ESAM TSLPR • • CD122 • • • • Mac-2 • CD8b • CD192 • F4/80 VEGFR-3 CD57 • CD123 • • CD290 • • Mac-3 • • CD9 • • CD58 CD193 • • CD294 FcεRIα • CD124 • • • • • MAdCAM-1 • VISTA • CD10 • CD59 CD194 • • CD298 FPR3 • CD126 • • • • MAIR-IV • XCR1 • • CD11a • • CD59a CD195 • • CD300c FR4 • CD127 • • • • MAIR-V • β2- CD11b • • CD61 CD196 • • CD300c/d Galectin-9 microglobulin • • • CD129 • • • MD-1 • CD11c • • CD62E CD197 • • Ganglioside Isotype Controls • CD130 • • CD300LG • • MERTK • • CD13 • CD62L • • CD198 • • GD2 Armenian CD131 • CD301 • • MICA/MICB • CD14 CD199 GARP • • Hamster IgG • • • • CD62P • CD132 • • CD301b • • • MRGX2 • Isotype Ctrl CD15 • CD63 • • CD200 • • GITR Ligand • CD133 • CD303 • MSC • Mouse IgG1, κ CD16 • CD64 • • CD200R • • GL7 • • • CD134 • • CD304 • • MSC,NPC • Isotype Ctrl CD16/32 • CD66a • CD200R3 • GPR19 • CD135 • • CD307e • MUC-13 • Mouse IgG2a, CD18 • • CD66a/c/e • CD201 • GPR56 • • • CD137 • • CD309 • • NKG2D • κ Isotype Ctrl CD19 • • CD66b • CD202b • • GPR83 • Mouse IgG2b, CD138 • • CD314 • NKp80 • • • CD20 • • CD69 • • CD203c H-2 • κ Isotype Ctrl CD140a • • • CD317 • • NK-1.1 • CD21 • CD70 • • CD205 Mouse IgG3, κ CD140b • • • • CD318 • HLA-A,B,C • Notch 1 • • • CD21, CD35 • CD71 Isotype Ctrl • • CD141 • CD206 • CD319 • • HLA-DR • Notch 2 CD22 • • Mouse IgM, κ • • CD73 • • CD207 • • CD142 • • • CD323 • HLA-E • Notch 3 • • Isotype Ctrl CD23 • • CD74 • CD143 • CD209 • CD324 • HVEM • • Notch 4 • • Rat IgG1, κ CD24 • • CD79b • • • • CD144 • • CD210 • • CD325 • NPC • Isotype Ctrl CD25 • • I-A/I-E • CD80 • • CD146 CD213α1 • CD326 • • Rat IgG2a, κ • • IFNAR-1 PDC-TREM • • • CD26 • CD81 • • • Isotype Ctrl CD147 • CD213α2 • CD328 • PD-1H • CD27 • • IFN-γ R β Rat IgG2b, κ CD82 • CD215 CD334 CD148 • • • chain • • PIR-A/B • • • CD28 • • CD83 • • Isotype Ctrl CD150 • • CD217 • CD335 • • Ig light Plexin B2 • CD29 • • CD84 • • • • Rat IgG2c, κ CD151 • CD218a • CD336 • chain κ Podoplanin • • Isotype Ctrl • CD30 • • CD85 • CD152 • • CD220 • CD337 • Ig light PSMA • Rat IgM, κ CD31 • • CD85d • • • • CD153 • CD221 • CD338 • chain λ RAE-1γ • Isotype Ctrl CD32 • CD85h • CD154 • • CD223 • • CD339 • IgD • • ROR1 • Syrian CD33 • CD85k • • CD226 CD340 • IgG Fc • Sialyl Lewis X Hamster IgG • CD34 CD155 • • • • • • CD86 • • (dimeric) • Isotype Ctrl CD156c • CD227 • CD344 • IgM • • CD35 • CD87 • Siglec H • CD229 • • CD351 • IL-21R • CD36 • • CD88 CD157 • • • CD352 Siglec-8 • CD158 CD230 (Prion) • IL-23R • CD36L1 • CD89 • • • Siglec-9 CD354 • • CD37 • CD90 • CD158b • CD231 • IL-28RA • Siglec-10 • CD355 • • CD38 • • CD90.1 • CD158e1 • CD235ab • IL-33Rα • SSEA-1 • CD39 • • CD90.2 CD243 CD357 • • SSEA-3 • CD159a • • Integrin α9β1 • • CD40 • • CD92 CD360 SSEA-4 • CD160 CD244 (2B4) • • Integrin β5 • • CD41 • • • • CD93 • • CD365 SSEA-5 • CD161 CD245 • • • Integrin β7 • • CD42b • CD94 • • • CD366 SUSD2 • CD162 CD252 • • • • Jagged 2 • • CD43 • • CD95 • • TACSTD2 • CD253 CD368 CD44 • • CD96 • • CD163 • • • JAML • TCR Vγ1.1 + • CD45 • • CD97 • CD164 • CD254 • • CD369 • • KLRG1 • • Vγ1.2 This list may be subject to change. For updated specificity list, visit:biolegend.com/legendscreen Toll-Free Tel: (US & Canada): 1.877.BIOLEGEND (246.5343) Tel: 858.768.5800 biolegend.com

07-0054-03

World-Class Quality | Superior Customer Support | Outstanding Value