hP2RX2 VersaClone cDNA Catalog Number: RDC0514

Specifications: Description This shuttle vector contains the complete ORF for the of interest, Gene: hP2RX2 along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with Accession: NP_777362 convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector. Insert size: 1062bp Preparation and Storage Concentration: 10µg at 0.2μg/μL Formulation cDNA is provided in 10 mM Tris-Cl, pH 8.5

Shipping Ships at ambient temperature

Stability 1 year from date of receipt when stored at -20°C to -80°C Storage Use a manual defrost freezer and avoid repeated hP2RX2 cDNA freeze-thaw cycles.

Plasmid

P2RX2 purinergic P2X,

-gated , 2 PfoI BstAPI [ Homo sapiens ] SspI NdeI HpaI HindIII EcoRV XmnI BstXI BmtI Also known as: P2X2 NheI NotI PpuMI EagI Summary: TsoI BtgI SnaBI NcoI P2RX2 belongs to the family of DraIII BseRI purinoceptors for ATP. It functions AMP as a ligand-gated ion channel. PasI NmeAIII AarI Purinergic receptors are also BpmI BsaI RDC0514 Bsu36I expressed in spinal cord neurons hP2RX2 (1-349) and are known to cross-talk with 3796 bps AhdI Van91I GABA(A)Rs. Binding to ATP mediates BtgZI synaptic transmission between Acc65I neurons and from neurons to KpnI smooth muscle. Six transcript AleI EcoNI variants encoding six distinct COLE1 AccIII isoforms have been identified for PmlI AscI AlwNI MscI BssHII this gene. PspXI Bst1107I XhoI XbaI SalI PciI SapI EcoRI

FOR RESEARCH USE ONLY

NOT FOR USE IN HUMANS

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> RDC0514 Plasmid DNA Sequence 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 201 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 401 ggagacgtgt taacaagctt ggatccgata tcgctagcgc ggccgccacc atggccgccg cccagcccaa gtaccccgcc ggggcgaccg cccggcgcct 501 ggcccggggc tgctggtccg ccctctggga ctacgagacg cccaaggtga tcgtggtgag gaaccggcgc ctgggggtcc tgtaccgcgc cgtgcagctg 601 ctcatcctgc tctacttcgt gtggtacgta ttcatcgtgc agaaaagcta ccaggagagc gagacgggcc ccgagagctc catcatcacc aaggtcaagg 701 ggatcaccac gtccgagcac aaagtgtggg acgtggagga gtacgtgaag ccccccgagg ggggcagcgt gttcagcatc atcaccaggg tcgaggccac 801 ccactcccag acccagggaa cctgccccga gagcataagg gtccacaacg ccacctgcct ctccgacgcc gactgcgtgg ctggggagct ggacatgctg 901 ggaaacggcc tgaggaccgg gcgctgtgtg ccctattacc aggggccctc caagacctgc gaggtgttcg gctggtgccc ggtggaagat ggggcctctg 1001 tcagccaatt tctgggtacg atggccccaa atttcaccat cctcatcaag aacagcatcc actaccccaa attccacttc tccaagggca acatcgccga 1101 ccgcacagac gggtacctga agcgctgcac gttccacgag gcctccgacc tctactgccc catcttcaag ctgggcttta tcgtggagaa ggctggggag 1201 agcttcacag agctcgcaca caagggtggt gtcatcgggg tcattatcaa ctgggactgt gacctggacc tgcctgcatc ggagtgcaac cccaagtact 1301 ccttccggag gcttgacccc aagcacgtgc ctgcctcgtc aggctacaac ttcaggtttg ccaaatacta caagatcaat ggcaccacca cccgcacgct 1401 catcaaggcc tacgggatcc gcattgacgt cattgtgcat ggacaggccg ggaagttcag cctgattccc accattatta atctggccac agctctgtaa 1501 ggcgcgccag tatactctag agtcgacacc cggggaattc ctcgagcgct cgtctctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 1601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 1701 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 1801 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 2001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 2101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 2201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 2301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 2401 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 2501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 2601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 2701 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 2801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2901 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 3001 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 3101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3201 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 3301 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 3401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 3601 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 3701 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc

> RDC0514 Translated Insert Sequence 1 maaaqpkypa gatarrlarg cwsalwdyet pkvivvrnrr lgvlyravql lillyfvwyv fivqksyqes etgpessiit kvkgittseh kvwdveeyvk 101 ppeggsvfsi itrveathsq tqgtcpesir vhnatclsda dcvageldml gnglrtgrcv pyyqgpsktc evfgwcpved gasvsqflgt mapnftilik 201 nsihypkfhf skgniadrtd gylkrctfhe asdlycpifk lgfivekage sftelahkgg vigviinwdc dldlpasecn pkysfrrldp khvpassgyn 301 frfakyykin gtttrtlika ygiridvivh gqagkfslip tiinlatal

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