Anti-PRR5 Produced in Rabbit, Affinity Isolated Antibody

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Anti-PRR5 produced in rabbit, affinity isolated antibody Catalog Number SAB4200313 Product Description Precautions and Disclaimer Anti-PRR5 is produced in rabbit using as immunogen a This product is for R&D use only, not for drug, synthetic peptide corresponding to an internal region of household, or other uses. Please consult the Material human PRR5 (GeneID: 55615), conjugated to KLH. Safety Data Sheet for information regarding hazards The corresponding sequence is identical in mouse and and safe handling practices. rat. The antibody is affinity-purified using the immunizing peptide immobilized on agarose. Storage/Stability For continuous use, store at 2-8 °C for up to one Anti-PRR5 recognizes human PRR5. The antibody may month. For extended storage freeze in working be used in various immunochemical techniques aliquots. Repeated freezing and thawing is not including immunoblotting (~45/50 kDa). Detection of the recommended. If slight turbidity occurs upon prolonged PRR5 bands by immunoblotting is specifically inhibited storage, clarify the solution by centrifugation before by the immunizing peptide. use. Working dilution samples should be discarded if not used within 12 hours. PRR5 (proline-rich protein 5), also known as Protor-1, is a conserved proline-rich protein expressed Product Profile predominantly in kidney. The PRR5 gene is located in a Immunoblotting: a working concentration of region of chromosome 22 reported to contain a tumor 0.5-1.0 mg/mL is recommended using whole extracts of suppressor gene that may be involved in breast and HEK-293T cells over expressing human PRR5. colorectal tumorigenesis. PRR5 is a component of the mammalian target of rapamycin complex 2 (mTORC2), Note: In order to obtain best results in different and it regulates platelet-derived growth factor (PDGF) techniques and preparations we recommend receptor b expression and PDGF signaling to Akt and determining optimal working concentration by titration S6K1. Alternative splicing and the use of alternative test. promoters result in transcripts encoding different isoforms. Read-through transcripts from this gene into References the downstream Rho GTPase activating protein 8 1. Johnstone, C.N., et al., Genomics, 85, 338-351 (ARHGAP8) gene also exist, which led to the original (2005). description of PRR5 and ARHGAP8 being a single 2. Woo, S.Y., et al., J. Biol. Chem., 282, 25604- gene.1-3 25612 (2007). 3. Pearce, L.R., et al., Biochem. J., 405, 513-522 Reagent (2007). Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative. ST,RC,KAA,PHC 07/11-1 Antibody Concentration: ~ 1.0 mg/mL Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip..

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