U.O.No. 5455/2021/Admn Dated, Calicut University.P.O, 21.05.2021
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'Fuyu' Persimmon Fruits
Scientia Horticulturae 172 (2014) 292–299 Contents lists available at ScienceDirect Scientia Horticulturae journal homepage: www.elsevier.com/locate/scihorti The accumulation of tannins during the development of ‘Giombo’ and ‘Fuyu’ persimmon fruits ∗ Magda Andréia Tessmer, Ricardo Alfredo Kluge, Beatriz Appezzato-da-Glória University of São Paulo/ESALQ, Department of Biological Sciences, C.P. 13418-900 Piracicaba, SP, Brazil a r t i c l e i n f o a b s t r a c t Article history: Tannins are responsible for the astringency of persimmon fruits. The present study compared the develop- Received 7 November 2013 ment of ‘Giombo’ (PVA) and ‘Fuyu’ (PCNA) persimmon fruits until maturity to determine if the difference Received in revised form 16 April 2014 in astringency between the two cultivars is related to the early accumulation of tannins in the cells or Accepted 18 April 2014 to differences in the pattern of tannin accumulation during cell differentiation, cell density or the con- centrations of total and soluble tannins. Persimmon flowers and fruits were collected from a commercial Keywords: orchard in Mogi das Cruzes during the 2010–2011 harvest season at predetermined stages until the fruit Anatomy reached commercial maturity. Structural analyses were performed by light, scanning and transmission Astringency electron microscopy, and quantitative analyses of tannin cell density, tannin cell index and total and Diospyros kaki L. soluble tannins were also performed. In both cultivars, the ovary already exhibited a small number of Tannin cells tannin cells. Throughout development, the ‘Giombo’ persimmon possessed higher tannin cell density and higher levels of total and soluble tannins compared to ‘Fuyu’. -
Abnormal Pap Smear
9195 Grant Street, Suite 410 300 Exempla Circle, Suite 470 6363 West 120th Avenue, Suite 300 Thornton, CO 80229 Lafayette, CO 80026 Broomfield, CO 80020 Phone: 303-280-2229(BABY) 303-665-6016 303-460-7116 Fax: 303-280-0765 303-665-0121 303-460-8204 www.whg-pc.com Abnormal Pap Smear The Pap test (also called a Pap smear) is a way to examine cells collected from the cervix and vagina. This test can show the presence of infection, inflammation, abnormal cells, or cancer. Regular Pap tests are an important step to the prevention of cervical cancer. Approximately 15,000 American women are diagnosed with cervical cancer each year and about 5,000 die of the disease. In areas of the world where Pap tests are not widely available, cervical cancer is a leading cause of cancer deaths in women. A Pap smear can assist your doctor in catching cervical cancer early. Early detection of cervical dysplasia (abnormal cells on the cervix) and treatment are the best ways to prevent the development of cervical cancer. What do abnormal Pap smear results mean? Abnormal Pap smear results can indicate mild or serious abnormalities. Most abnormal cells on the surface of the cervix are not cancerous. It is important to remember that abnormal conditions do not always become cancerous, and some conditions are more of a threat than others. There are several terms that may be used to describe abnormal results. • Dysplasia is a term used to describe abnormal cells. Dysplasia is not cancer, although it may develop into cancer of the cervix if not treated. -
Post-Zygotic Mosaic Mutation in Normal Tissue from Breast Cancer Patient
Extended Abstract Research in Genes and Proteins Vol. 1, Iss. 1 2019 Post-zygotic Mosaic Mutation in Normal Tissue from Breast Cancer Patient Ryong Nam Kim Seoul National University Bio-MAX/NBIO, Seoul, Korea, Email: [email protected] ABSTRACT Even though numerous previous investigations had shed errors during replication, defects in chromosome fresh light on somatic driver mutations in cancer tissues, segregation during mitosis, and direct chemical attacks by the mutation-driven malignant transformation mechanism reactive oxygen species. the method of cellular genetic from normal to cancerous tissues remains still mysterious. diversification begins during embryonic development and during this study, we performed whole exome analysis of continues throughout life, resulting in the phenomenon of paired normal and cancer samples from 12 carcinoma somatic mosaicism. New information about the genetic patients so as to elucidate the post-zygotic mosaic diversity of cells composing the body makes us reconsider mutation which may predispose to breast carcinogenesis. the prevailing concepts of cancer etiology and We found a post-zygotic mosaic mutation PIK3CA pathogenesis. p.F1002C with 2% variant allele fraction (VAF) in normal tissue, whose respective VAF during a matched carcinoma Here, I suggest that a progressively deteriorating tissue, had increased by 20.6%. Such an expansion of the microenvironment (“soil”) generates the cancerous “seed” variant allele fraction within the matched cancer tissue and favors its development. Cancer Res; 78(6); 1375–8. ©2018 AACR. Just like nothing ha s contributed to the may implicate the mosaic mutation in association with the causation underlying the breast carcinogenesis. flourishing of physics quite war, nothing has stimulated the event of biology quite cancer. -
Genetic Mosaicism: What Gregor Mendel Didn't Know
Genetic mosaicism: what Gregor Mendel didn't know. R Hirschhorn J Clin Invest. 1995;95(2):443-444. https://doi.org/10.1172/JCI117682. Research Article Find the latest version: https://jci.me/117682/pdf Genetic Mosaicism: What Gregor Mendel Didn't Know Editorial The word "mosaic" was originally used as an adjective to depending on the developmental stage at which the mutation describe any form of work or art produced by the joining to- occurs, may or may not be associated with somatic mosaicism gether of many tiny pieces that differ in size and color (1). In and may include all or only some of the germ cells. (A totally that sense, virtually all multicellular organisms are mosaics of different mechanism for somatic mosaicism has been recently cells of different form and function. Normal developmentally described, reversion of a transmitted mutation to normal [4]. determined mosaicism can involve permanent alterations of We have additionally identified such an event [our unpublished DNA in somatic cells such as the specialized cells of the im- observations]. ) mune system. In such specialized somatic cells, different re- Somatic and germ line mosaicism were initially inferred on arrangements of germ line DNA for immunoglobulin and T cell clinical grounds for a variety of diseases, including autosomal receptor genes and the different mutations accompanying these dominant and X-linked disorders, as presciently reviewed by rearrangements alter DNA and function. However, these alter- Hall (3). Somatic mosaicism for inherited disease was initially ations in individual cells cannot be transmitted to offspring definitively established for chromosomal disorders, such as since they occur only in differentiated somatic cells. -
Phenotype Manifestations of Polysomy X at Males
PHENOTYPE MANIFESTATIONS OF POLYSOMY X AT MALES Amra Ćatović* &Centre for Human Genetics, Faculty of Medicine, University of Sarajevo, Čekaluša , Sarajevo, Bosnia and Herzegovina * Corresponding author Abstract Klinefelter Syndrome is the most frequent form of male hypogonadism. It is an endocrine disorder based on sex chromosome aneuploidy. Infertility and gynaecomastia are the two most common symptoms that lead to diagnosis. Diagnosis of Klinefelter syndrome is made by karyotyping. Over years period (-) patients have been sent to “Center for Human Genetics” of Faculty of Medicine in Sarajevo from diff erent medical centres within Federation of Bosnia and Herzegovina with diagnosis suspecta Klinefelter syndrome, azoo- spermia, sterilitas primaria and hypogonadism for cytogenetic evaluation. Normal karyotype was found in (,) subjects, and karyotype was changed in (,) subjects. Polysomy X was found in (,) examinees. Polysomy X was expressed at the age of sexual maturity in the majority of the cases. Our results suggest that indication for chromosomal evaluation needs to be established at a very young age. KEY WORDS: polysomy X, hypogonadism, infertility Introduction Structural changes in gonosomes (X and Y) cause different distribution of genes, which may be exhibited in various phenotypes. Numerical aberrations of gonosomes have specific pattern of phenotype characteristics, which can be classified as clini- cal syndrome. Incidence of gonosome aberrations in males is / male newborn (). Klinefelter syndrome is the most common chromosomal disorder associated with male hypogonadism. According to different authors incidence is / male newborns (), /- (), and even / (). Very high incidence indicates that the zygotes with Klinefelter syndrome are more vital than those with other chromosomal aberrations. BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2008; 8 (3): 287-290 AMRA ĆATOVIĆ: PHENOTYPE MANIFESTATIONS OF POLYSOMY X AT MALES In , Klinefelter et al. -
Gênero Closterium (Closteriaceae) Na Comunidade Perifítica Do Reservatório De Salto Do Vau, Sul Do Brasil
Gênero Closterium (Closteriaceae) ... 45 Gênero Closterium (Closteriaceae) na comunidade perifítica do Reservatório de Salto do Vau, sul do Brasil Sirlene Aparecida Felisberto & Liliana Rodrigues Universidade Estadual de Maringá, PEA/Nupélia. Av. Colombo, 3790, Maringá, Paraná, Brasil. [email protected] RESUMO – Este trabalho objetivou descrever, ilustrar e registrar a ocorrência de Closterium na comunidade perifítica do reservatório de Salto do Vau. As coletas do perifíton foram realizadas no período de verão e inverno, em 2002, nas regiões superior, intermediária e lacustre do reservatório. Os substratos coletados na região litorânea foram de vegetação aquática, sempre no estádio adulto. Foram registradas 23 espécies pertencentes ao gênero Closterium, com maior número para o período de verão (22) do que para o inverno (11). A maior riqueza de táxons foi registrada na região lacustre do reservatório no verão e na intermediária no inverno. As espécies melhor representadas foram: Closterium ehrenbergii Meneghini ex Ralfs var. immane Wolle, C. incurvum Brébisson var. incurvum e C. moniliferum (Bory) Ehrenberg ex Ralfs var. concavum Klebs. Palavras-chave: taxonomia, Closteriaceae, algas perifíticas, distribuição longitudinal. ABSTRACT – Genus Closterium (Closteriaceae) in periphytic community in Salto do Vau Reservoir, southern Brazil. The aim of this study was to describe, illustrate and to register the occurrence of Closterium in the periphytic community in Salto do Vau reservoir. The samples were collected in the summer and winter periods, during 2002. Samples were taken from natural substratum of the epiphyton type in the adult stadium. Substrata were collected in three regions from the littoral region (superior, intermediate, and lacustrine). In the results there were registered 23 species in the Closterium, with 22 registered in the summer and 11 in the winter period. -
Esmo Advanced Course on Ntrk Gene Fusion
ESMO ADVANCED COURSE ON NTRK GENE FUSION Mechanisms of gene fusion, fusion partners and consequences in oncogenesis Description, Structure and function of TRK and NTRK in ontogenesis Mehdi Brahmi Lyon, 13-14 September 2019 DISCLOSURE OF INTEREST No conflict of interest 1. Mechanisms of gene fusion 2. Gene fusion partners 3. Consequences in oncogenesis INTRODUCTION • Gene fusions (chromosomal rearrangements) • Juxtapositioning of 2 previously independent genes • From 2 nonhomologous chromosomes • Hybrid genes Translation of deregulated and/or chimeric protein • Most common type of structural chromosomal abnormalities • Not exclusive to cancer cells • Screening of cells from developing embryos • Translocations in 0.7 per 1000 live births = de novo • +/- associated to developmental abnormalities in some cases (unbalanced translocations) • Particularly common in cancer cells • Total number of gene fusions > 10 000 • > 90% identified in the past 5 years (deep-sequencing) • Listed in databases • COSMIC; http://www.sanger.ac.uk/genetics/CGP/cosmic/; dbCRID; http://dbcrid.biolead.org GENOMIC ALTERATIONS IN CANCER Drivers vs Passengers • High rate of chromosomal rearrangements in cancer • Most solid tumors • Changes in chromosome number (aneuploidy) • Deletions, inversions, translocations • Other genetic abnormalities • Drivers or Passengers • Passengers > Drivers • Alterations in non coding regions • Easy survival because mutations (TP53, etc…) • Statistical methods to identify “driver genes” • Frequency, Reccurence • Predicted effects « DRIVER -
Sperm Aneuploidy and DNA Fragmentation in Unexplained
Esquerré-Lamare et al. Basic and Clinical Andrology (2018) 28:4 https://doi.org/10.1186/s12610-018-0070-6 RESEARCH ARTICLE Open Access Sperm aneuploidy and DNA fragmentation in unexplained recurrent pregnancy loss: a multicenter case-control study Camille Esquerré-Lamare1,2, Marie Walschaerts1,2, Lucie Chansel Debordeaux3, Jessika Moreau1,2, Florence Bretelle4, François Isus1,5, Gilles Karsenty6, Laetitia Monteil7, Jeanne Perrin8,9, Aline Papaxanthos-Roche3 and Louis Bujan1,2* Abstract Background: Recurrent pregnancy loss (RPL) is defined as the loss of at least three pregnancies in the first trimester. Although the most common cause is embryo aneuploidy, and despite female checkup and couple karyotyping, in about 50% of cases RPL remain unexplained. Male implication has little been investigated and results are discordant. In this context, we conducted a multi-center prospective case-control study to investigate male gamete implication in unexplained RPL. Methods: A total of 33 cases and 27 controls were included from three university hospitals. We investigated environmental and family factors with a detailed questionnaire and andrological examination, sperm characteristics, sperm DNA/chromatin status using the sperm chromatin structure assay (SCSA) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and sperm aneuploidy using fluorescence in situ hybridization (FISH). The Mann-Whitney test and the Wilcoxon or Fisher exact tests were used. A non-parametric Spearman correlation was performed in order to analyze the relationship between various sperm parameters and FISH and sperm DNA fragmentation results. Results: We found significant differences between cases and controls in time to conceive, body mass index (BMI), family history of infertility and living environment. -
ASCO Answers: Testicular Cancer
Testicular Cancer What is testicular cancer? Testicular cancer begins when healthy cells in 1 or both testicles change and grow out of control, forming a mass called a tumor. Most testicular tumors develop in germ cells, which produce sperm. These tumors are called germ cell tumors and are divided into 2 types: seminoma or non- seminoma. A non-seminoma grows more quickly and is more likely to spread than a seminoma, but both types need immediate treatment. What is the function of the testicles? The testicles, also called the testes, are part of a man’s reproductive system. Each man has 2 testicles. They are located under the penis in a sac-like pouch called the scrotum. The testicles make sperm and testosterone. Testosterone is a hormone that plays a role in the development of a man’s reproductive organs and other characteristics. What does stage mean? The stage is a way of describing where the cancer is located, if or where it has spread, and whether it is affecting other parts of the body. There ONCOLOGY. CLINICAL AMERICAN SOCIETY OF 2004 © LLC. EXPLANATIONS, MORREALE/VISUAL ROBERT BY ILLUSTRATION are 4 stages for testicular cancer: stages I through III (1 through 3) plus stage 0 (zero). Stage 0 is called carcinoma in situ, a precancerous condition. Find more information about these stages at www.cancer.net/testicular. How is testicular cancer treated? The treatment of testicular cancer depends on the type of tumor (seminoma or non-seminoma), the stage, the amount of certain substances called serum tumor markers in the blood, and the person’s overall health. -
Evaluation of Water Quality in Aquatic Ecosystems - Harukuni Tachibana, Toshiro Maruyama, Yasumoto Magara
WATER QUALITY AND STANDARDS – Vol. I - Evaluation of Water Quality in Aquatic Ecosystems - Harukuni Tachibana, Toshiro Maruyama, Yasumoto Magara EVALUATION OF WATER QUALITY IN AQUATIC ECOSYSTEMS Harukuni Tachibana Hokkaido University, Sapporo, Japan Toshiro Maruyama Miyazaki University, Miyazaki, Japan Yasumoto Magara Hokkaido University, Sapporo, Japan Keywords: Algal bioassay, Ecosystem, Monochloramine, POPs, Saprobity system, Water quality standard, Zinc Contents 1. Introduction 2. Saprobity system 3. Hazardous chemical management for protecting aqua ecosystem 4. Effects of chlorine-disinfected wastewater on the growth of Nori Glossary Bibliography Biographical Sketches Summary To maintain the water environment in a favorable state, a sound and stable ecosystem is essential. In other words, ecosystems are qualitative indicators of the water environment because their formation depends on that environment. In studies on the relationship between water environment and ecosystems, Kolkwitz and Marrson summarized, in 1909, the relation between the aquatic environment and ecology as a saprobity system of water quality. The biological indicator table, created based on the saprobity system, categorizes biological, physical, and chemical characteristics according to a four-group hierarchy of water quality pollution. UNESCO – EOLSS Because of the evidence that species structure of the diatom community is affected by the organic pollution level of the river in where located in middle to high latitude regions, the DiatomSAMPLE Assemblage Index of pollutionCHAPTERS (DAIpo) is applied to give the numerical parameter of the organic pollution level. These general phenomena of conventional water pollution such as oxygen depletion, contamination, acute toxicity, etc., which relate directly to factors responsible for life and death of biota, are assessed by the saprobity system. -
Sex Chromosome Aneuploidies
7 Sex Chromosome Aneuploidies Eliona Demaliaj1, Albana Cerekja2 and Juan Piazze3 1Department of Obstetric-Gynecology, Faculty of Medicine, University of Tirana Hospital "Mbreteresha Geraldine", Tirane 2Gynecology and Obstetrics Ultrasound Division, ASL Roma B, Rome 3Ultrasound Division, Ospedale di Ceprano/Ospedale SS. Trinità di Sora, Frosinone 1Albania 2,3Italy 1. Introduction Sex chromosome aneuploidy is defined as a numeric abnormality of an X or Y chromosome, with addition or loss of an entire X or Y chromosome. Sex chromosome mosaicism, in which one or more populations of cells have lost or gained a sex chromosome, also is common. The most commonly occurring sex chromosome mosaic karyotypes include 45,X/46XX, 46XX/47,XXX, and 46,XY/47,XXY. Less frequent are those sex chromosome abnormalities where addition of more than one sex chromosome or a structural variant of an X or Y chromosome occur. The X chromosome is one of the two sex-determining chromosomes in many animal species, including mammals and is common in both males and females. It is a part of the XY and X0 sex-determination system. The X chromosome in humans represents about 2000 out of 20,000 - 25,0000 genes. Normal human females have 2 X-chromosomes (XX), for a total of nearly 4000 "sex-tied" genes (many of which have nothing to do with sex, other than being attached to the chromosome that is believed to cause sexual bimorphism (or polymorphism if you count more rare variations). Men have, depending on the study, 1400-1900 fewer genes, as the Y chromosome is thought to have only the remaining genes down from an estimated 1438 -~2000 (Graves 2004). -
Understanding the Microbial Ecology of Highly Radioactive Nuclear Storage Facilities
Understanding the microbial ecology of highly radioactive nuclear storage facilities A thesis submitted to the University of Manchester for the degree of Doctor of Philosophy in the Faculty of Science and Engineering 2018 Lynn Foster School of Earth and Environmental Sciences 1 Contents List of Figures……………………………………………………………………..7 List of Tables……………………………………………………………………..13 List of Equations…………………………………………………………………14 List of abbreviations……………………………………………………………...15 Abstract…………………………………………………………………………..17 Declaration……………………………………………………………………….18 Copyright statement……………………………………………………………...19 Acknowledgements………………………………………………………………20 The author………………………………………………………………………...22 1 Introduction ........................................................................................ 24 1.1 Project context .................................................................................................. 24 1.2 Aims and objectives ......................................................................................... 25 1.3 Thesis structure................................................................................................. 27 1.4 Author contributions......................................................................................... 28 2 Literature review ................................................................................ 32 2.1 The nuclear fuel cycle ...................................................................................... 32 2.1.1 The fuel cycle ...........................................................................................