DMD Fast Forward. Published on December 15, 2019 as DOI: 10.1124/dmd.119.089482 This article has not been copyedited and formatted. The final version may differ from this version. DMD #89486 Use of Phenotypically Poor Metaboliser Individual Donor Human Liver Microsomes to Identify Selective Substrates of UGT2B10 Nicolo Milani, Nahong Qiu, Birgit Molitor, Justine Badée, Gabriele Cruciani and Stephen Fowler Pharmaceutical Sciences, Roche Pharma Research and Early Development, Roche Innovation Centre Downloaded from Basel, Grenzacherstrasse 124, 4070, Basel, Switzerland (NM, NQ, BM, SF); Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italy (NM, GC), Department of Pharmaceutics, Center for Pharmacometrics and Systems Pharmacology, University dmd.aspetjournals.org of Florida at Lake Nona, Orlando, Florida, USA (JB)* *Current Address: Modelling and Simulation, PK Sciences, Novartis Institutes for BioMedical Research, CH-4002, Basel, Switzerland. at ASPET Journals on September 23, 2021 1 DMD Fast Forward. Published on December 15, 2019 as DOI: 10.1124/dmd.119.089482 This article has not been copyedited and formatted. The final version may differ from this version. DMD #89486 Running title: Rapid detection of selective UGT2B10 substrates Corresponding author: Stephen Fowler, Pharmaceutical Sciences, Roche Pharma Research and Early Development, Roche Innovation Centre Basel, Grenzacherstrasse 124, 4070, Basel, Switzerland. Phone: +41 61 688 5105. Email:
[email protected] Downloaded from Abbreviations: HLM: human liver microsomes, Km: Michaelis-Menten constant, MgCl2: magnesium chloride, LC: dmd.aspetjournals.org liquid chromatography, MS/MS: tandem mass spectrometry, UDPGA: uridine 5’- diphosphoglucuronic acid, UGT: Uridine 5’-diphospho (UDP)-glucuronosyltransferases, UHPLC: ultra-high performance liquid chromatography, NADPH: Nicotinamide adenine dinucleotide at ASPET Journals on September 23, 2021 phosphate, MIFs: Molecular Interaction Fields, Clint: intrinsic clearance, S.D.: standard deviation.