LDB3 Rabbit Pab

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LDB3 Rabbit pAb

Catalog No.: A7462

Basic Information Background

Catalog No. This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein- A7462 protein interaction domains consisting of 80-120 amino acid residues. PDZ domain- containing proteins interact with each other in cytoskeletal assembly or with other Observed MW proteins involved in targeting and clustering of membrane proteins. The protein 50kDa/90kDa encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine- Calculated MW rich motif defined by 50-60 amino acids containing two zinc-binding modules. This 30kDa/35kDa/42kDa/50kDa/66kDa/77 protein also interacts with all three members of the myozenin family. Mutations in this kDa/78kDa gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been Category identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 11155 O75112 IHC 1:50 - 1:200 Immunogen IF 1:50 - 1:200 Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human LDB3 (NP_001073585.1).

Synonyms LDB3;CMD1C;CMH24;CMPD3;CYPHER;LDB3Z1;LDB3Z4;LVNC3;MFM4;ORACLE;PDLIM6;ZAS P

Contact Product Information

Source Isotype Purification www.abclonal.com Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using LDB3 antibody (A7462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Immunohistochemistry of paraffin- Immunofluorescence analysis of human Immunofluorescence analysis of rat heart embedded mouse stomach using LDB3 placenta cells using LDB3 antibody (A7462) cells using LDB3 antibody (A7462) at Rabbit pAb (A7462) at dilution of 1:200 at dilution of 1:100. Blue: DAPI for nuclear dilution of 1:100. Blue: DAPI for nuclear (40x lens). staining. staining.

Immunofluorescence analysis of mouse heart cells using LDB3 antibody (A7462) at dilution of 1:100. Blue: DAPI for nuclear staining.