New Records of Mosquito Species in Northwestern Argentina

Journal of the American Mosquito Control Association, 36(3):201–203, 2020 Copyright Ó 2020 by The American Mosquito Control Association, Inc. SCIENTIFIC NOTE

NEW RECORDS OF MOSQUITO SPECIES IN NORTHWESTERN ARGENTINA

MARIA J. DANTUR JURI,1,2 EDECIO I. VILLARROEL MARTINEZ,1 GABRIELA C. FLORES,1 MARINA STEIN3 4 AND MARIA A. MUREB SALLUM

ABSTRACT. Four mosquito species—Anopheles (Nyssorhynchus) galvaoi, An.(Anopheles) mediopunctatus, Culex (Culex) lahillei, and Uranotaenia (Uranotaenia) geometrica´ —were recorded for the first time in northwestern Argentina. In addition, Psorophora (Grabhamia) confinnis and Cx.(Melanoconion) pedroi are reported for the first time in Jujuy Province, while Aedes (Ochlerotatus) hastatus, Coquillettidia (Rhynchotaenia) hermanoi, An.(Ano.) Downloaded from http://meridian.allenpress.com/jamca/article-pdf/36/3/201/2696189/i8756-971x-36-3-201.pdf by guest on 01 October 2021 fluminensis, and An.(Ano.) punctimacula were reported for the first time in Tucuma´n Province.

KEY WORDS Culicidae, new records, Jujuy, Salta, Tucuma´n

Mosquito fauna of northwestern Argentina are located in a primitive forest or wild ecosystem. The updated, citing new records and extending the typical forest of the southern area consisted of geographical distribution of some mosquito species. Tipuana tipu (Benth.) and Enterolobium contortisi- Previous studies have reported 242 species belonging liquum (Vell.) Morong as the primary trees, but a to 23 genera in Argentina (Rossi 2015), which was large portion has been replaced by sugarcane later increased to 246 species (Stein et al. 2018). In (Saccharum officinarum L.), soybean (Glycine max northwestern Argentina a total of 174 species of L.), and citrus (Citrus limon (L.) Burm.) plantations mosquitoes have been reported, belonging to 14 (Dantur Juri et al. 2010, 2012). La Florida and genera: Mansonia, Psorophora, Sabethes, Toxorhyn- Sargento Moya, included in this area, are mainly chites, Culex, Haemagogus, Limatus, Lutzia, Wyeo- located in a residual primitive forest with modifica- myia, Uranotaenia, Coquillettidia, Aedeomyia, tions as a result of human activities. The climate is Aedes, and Anopheles. This increased the number subtropical and distinguished by 3 seasons: a warm of species in Tucuma´n Province to 57, in Salta and dry spring (September–December), a warm and Province to 96, and in Jujuy Province to 62 (Dantur rainy summer (January–April), and a temperate-cool Juri et al. 2012, Linares et al. 2016, Veggiani Aybar and humid season that represents the autumn–winter and Rossi 2017). period (May–August) (Brown and Grau 1995, Dantur Mosquito surveillance was conducted at different Juri et al. 2011). localities situated in the subtropical mountainous Adult mosquitoes (M ¼ male, F ¼ female) were rainforest, also called Yungas, of Salta, Jujuy, and collected using Centers for Disease Control and Tucuma´n provinces (Fig. 1). Collection sites were Prevention light traps baited with CO2 canisters, and located within 2 major areas (north and south) of the with mechanical aspirators, which were used to piedmont forest, a subtropical mountainous rain- capture specimens resting on vegetation. Mosquitoes forest. The northern area is characterized by the captured were sacrificed with dry ice and transported presence of trees, e.g., Calycophyllum multiflorum to the central laboratory of Unidad Ejecutora Lillo Griseb. and Phyllostylon rhamnoides (Poiss.) Tauber, (CONICET Fundacion´ Miguel Lillo [FML]) for with a dense shrub stratum up to 2 m high composed identification under a stereoscopic microscope using of lianas, climbers, and epiphytes. The typical Aguas keys by Lane (1953), Darsie (1985), Consoli and Blancas, El Oculto, San Ramon´ de la Nueva Ora´n, Louren¸co de Oliveira (1994), and Forattini (2002). G¨uemes, and Yuto are included in this area. It was Voucher specimens were deposited in the collection of considered these sampling sites to be natural habitats FML, Tucuma´n, Argentina. Generic and subgeneric abbreviations used in this paper are those defined by 1 Unidad Ejecutora Lillo (CONICET–Fundacion´ Mi- the Walter Reed Biosystematics Unit (WRBU 2001). guel Lillo), Miguel Lillo 251, CP 4000, San Miguel de Collection data included: material examined Tucuma´n-Tucuma´n, Argentina. (M.E.), locality, collection date, number of collected 2 Universidad Nacional de Chilecito, 9 de Julio No. specimens, collectors (Coll.), and taxonomists (Det.). 22, CP F5360CKB, Chilecito, La Rioja, Argentina. 3 Departamento de Entomolog´ıa, Instituto de Medicina The following species are reported for the first Regional, Universidad del Nordeste, CONICET, CCT- time from the northwestern region of Argentina: Nordeste, Avenida Las Heras 727, CP 3500, Resistencia, Anopheles (Anopheles) mediopunctatus (Theo- Chaco, Argentina. bald). Distribution: Corrientes, Entre R´ıos, Misiones, 0 4 Departamento de Epidemiologia, Faculdade de Santa Fe. M.E.: Salta, Aguas Blancas (22843 S, Saude Publica,´ Universidade de Sa˜o Paulo, Avenida Dr. 648210W), 26-I-2005, 1F, 08-VI-2005, 1F; Salta, El Arnaldo 715, 01246-904, Sa˜o Paulo, SP, Brazil. Oculto (238060S, 648310W), 06-VI-2005, 2F, 11-VII-

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2005, 3F; Coll.: Dantur Juri, Laci, and Vianconi; Det.: Dantur Juri and Stein. Medical relevance: this species is an important vector of Venezuelan equine encephalitis virus (VEEV) (Torres et al. 2017). Culex (Melanoconion) pedroi Sirivanakam and Belkin. Distribution: Chaco, Corrientes, Misiones, Salta. M.E.: Jujuy, Yuto, 26-X-2005, 436F; Coll.: Dantur Juri, Laci, and Vianconi; Det.: Villarroel Mart´ınez. Medical relevance: this species was reported as a major vector of VEEV in Panama´, Venezuela, and Colombia (Torres et al. 2017).

Tucuma´n Province: Downloaded from http://meridian.allenpress.com/jamca/article-pdf/36/3/201/2696189/i8756-971x-36-3-201.pdf by guest on 01 October 2021 Aedes (Ochlerotatus) hastatus Dyar. Distribution: Chaco, Corrientes, Jujuy, Misiones, Salta. M.E.: Tucuma´n, S. Moya, 9-IV-2003, 1F; Coll.: Dantur Juri, Laci, and Vianconi; Det.: Villarroel Mart´ınez. Fig. 1. Mosquito collection sites in 3 provinces in Anopheles (Anopheles) fluminensis Root. Distri- northwestern Argentina. Black dots indicate the collection bution: Corrientes, Jujuy, Misiones, Salta. M.E.: localities: 1 ¼ Aguas Blancas, 2 ¼ El Oculto, 3 ¼ San Tucuma´n, S. Moya, 24-V-2002, 1F, 14-V-2003, Ramon´ de la Nueva Ora´n, 4 ¼ G¨uemes, Salta Province; 5 ¼ 12F; Coll.: Dantur Juri and Orono;˜ Det.: Dantur Juri Yuto, Jujuy Province; 6 ¼ La Florida, 7 ¼ Sargento Moya, and Sallum Mureb. Tucuma´n Province. Anopheles (Anopheles) punctimacula Dyar and Knab. Distribution: Buenos Aires, Chaco, Corrientes, 2005, 1F; Tucuma´n, Sargento Moya (S. Moya) Misiones, Salta, Santa Fe. M.E.: Tucuma´n, S. Moya, (278130S, 658390W), 13-V-2003, 1F; Coll.: Dantur 25-V-2002, 3F, 05-III-2003, 1F, 12-V-2003, 4F; Juri, Laci, and Vianconi; Det.: Dantur Juri and Coll.: Dantur Juri and Orono;˜ Det.: Dantur Juri and Sallum Mureb. Medical relevance: natural infection Sallum Mureb. Medical relevance: this species is with malaria parasites was observed in Colombia, considered as a secondary vector of malaria in suspected to be a minor vector in that country Colombia (Gorham et al. 1973). (Gorham et al. 1973). Coquillettidia (Rhynchotaenia) hermanoi (Lane Anopheles (Nyssorhynchus) galvaoi Causey, and Coutinho). Distribution: Chaco, Corrientes, Deane, and Deane. Distribution: Chaco. M.E.: Salta, Misiones. M.E.: Tucuma´n, S. Moya, 26-I-2003; Aguas Blancas, 08-IX-2002, 1F, 26-VII-2004, 2F, 77F; Coll.: Dantur Juri and Orono;˜ Det.: Dantur Juri 11-V-2005, 2F; Salta, El Oculto, 09-V-2004, 3F, 18- and Stein. X-2005, 2F; Salta, San Ramon´ de la Nueva Ora´n These collection records update the list of species 0 0 (Ora´n) (2387 S, 64819 W), 12-XII-2003, 5F, 30-V- recorded from northwestern Argentina and increase 2004, 8F, 13-V-2005, 7F, 21-VIII-2005, 17F, 20-X- the total number of species in the region to 184. The 2005, 9F; Jujuy, Yuto (23860S, 648460W), 27-X-2005, increases in number of species by province are as 17F, 08-XII-2005, 42F; Tucuma´n, S. Moya, 26-IX- follows: Jujuy, 62 to 65; Salta, 96 to 100; and 2002, 2F, 13-V-2003, 6F, 01-VI-2003, 3F; Coll.: Tucuma´n, 57 to 64. Some species are of medical Dantur Juri, Laci, and Vianconi; Det.: Dantur Juri. relevance. Aedes hastatus may be involved in Culex (Culex) lahillei Bachmann and Casal. arbovirus transmission, e.g., Pixuna virus that was Distribution: Buenos Aires, Cordoba,´ Corrientes, isolated from this species collected in Tucuma´n Santa Fe. M.E.: Tucuma´n, La Florida (278130S, Province, Argentina (Pisano et al. 2010, Dantur Juri 658330W), 02-XI-2003, 7F; S. Moya, 11-VI-2003, et al. 2012). Psorophora confinnis belongs to a 2F, 12-VI-2003, 2F; Coll.: Dantur Juri and Orono;˜ complex of species of considerable importance as Det.: Villarroel Mart´ınez. vectors of arboviruses, e.g., VEEV. The VEEV Uranotaenia (Uranotaenia) geometrica Theobald. subtypes IC and IAB have been isolated from Ps. Distribution: Buenos Aires, Chaco, Corrientes, Entre confinnis (Turell et al. 2000, Torres et al. 2017). R´ıos, Misiones. M.E.: Salta, G¨uemes (248420S, Culex pedroi have been found infected with the 65850W), 8-III-2013, 1F; Coll.: Dantur Juri, Laci, VEEV subtype ID in the Magdalena Valley, and Carrizo; Det.: Villarroel Mart´ınez. Colombia, and this species has also been found infected with VEEV and eastern equine encephalitis The species listed below are the first records for virus (EEEV) in Puerto Almendras, Peru (Turell et al. Jujuy and Tucuma´n provinces, respectively. 2005). Brazil–Peru and the Panama–Argentina sub- Jujuy Province: types of EEEV were isolated from Cx. pedroi (Turell Psorophora (Grabhamia) confinnis (Lynch Arri- et al. 2005). balzaga). Distribution: Buenos Aires, Chaco, We thank Cristian Laci and Daniel Carrizo of the Cordoba,´ Corrientes, Entre R´ıos, Formosa, Misiones, National Coordination of Vectors Control, Argenti- Salta, Santa Fe, Tucuma´n. M.E.: Jujuy, Yuto, 8-XII- nean Ministry of Health, for their collaboration SEPTEMBER 2020 SCIENTIFIC NOTE 203 during the field collecting trips. This contribution Culicidae) from northwestern Argentina. Check List was supported by the National Agency of Scientific 12:1–8. https://doi.org/10.15560/12.4.1944 and Technological Promotion (FONCyT) and the Pisano MB, Dantur Juri MJ, Re´ V, Diaz LA, Farias A, National Council of Scientific and Technical Re- Sanchez-Seco MP, Tenorio A, Almiron W, Contigiani search (CONICET). We thank Eric D. Carter, M. 2010. Co-circulation of R´ıo Negro virus (RNV) and Pixuna virus (PIXV) in Tucuma´n Province, Argentina. Community and Global Health Concentration, Ge- Trop Med Int Health 15:865–868. ography Department, Macalester College, for revis- Rossi GC. 2015. Annotated checklist, distribution, and ing the text for the English grammar. taxonomic bibliography of the mosquitoes (Insecta: Diptera: Culicidae) of Argentina. 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