DOCSLIB.ORG

Electrostatic Shape Control of a Charged Molecular Membrane from Ribbon to Scroll

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Electrostatic Shape Control of a Charged Molecular Membrane from Ribbon to Scroll Electrostatic shape control of a charged molecular membrane from ribbon to scroll Changrui Gaoa,1, Sumit Kewalramania,1, Dulce Maria Valenciaa, Honghao Lia, Joseph M. McCourtb, Monica Olvera de la Cruza,b,c,2, and Michael J. Bedzyka,b,2 aDepartment of Materials Science and Engineering, Northwestern University, Evanston, IL 60208; bDepartment of Physics and Astronomy, Northwestern University, Evanston, IL 60208; and cDepartment of Chemistry, Northwestern University, Evanston, IL 60208 Edited by Lia Addadi, Weizmann Institute of Science, Rehovot, Israel, and approved September 22, 2019 (received for review August 12, 2019) Bilayers of amphiphiles can organize into spherical vesicles, nano- acids, nanoribbons were found to transform into helical ribbons as tubes, planar, undulating, and helical nanoribbons, and scroll-like the PA concentration was reduced (17) and into helical and cochleates. These bilayer-related architectures interconvert under twisted nanoribbons when the amino acid sequence was permuted suitable conditions. Here, a charged, chiral amphiphile (palmitoyl- (18). Helical assemblies have been previously used to template lysine, C16-K1) is used to elucidate the pathway for planar nano- semiconductor nanohelices (19). Despite the progress, the corre- ribbon to cochleate transition induced by salt (NaCl) concentration. lation between experimental conditions such as molecular design, In situ small- and wide-angle X-ray scattering (SAXS/WAXS), atomic ionic strength, pH, amphiphile concentration, and the attained force and cryogenic transmission electron microscopies (AFM and nanoribbon-related morphology are not fully established. There- cryo-TEM) tracked these transformations over angstrom to microme- fore, precise control of nanoribbon-related architecture requires ter length scales. AFM reveals that the large length (L) to width (W) further understanding of the delicate interplay between intermo- ratio nanoribbons (L/W > 10) convert to sheets (L/W → 1) before lecular interactions and elastic and interfacial energies. rolling into cochleates. A theoretical model based on electrostatic A recent theoretical study showed that for charged molecules, and surface energies shows that the nanoribbons convert to sheets tuning the range of electrostatic interactions could induce tran- via a first-order transition, at a critical Debye length, with 2 shallow sitions between different nanoribbon-related morphologies (20). minima of the order of thermal energy at L/W >> 1 and at L/W = 1. Specifically, a phase diagram was deduced for a 2D lattice of CHEMISTRY SAXS shows that interbilayer spacing (D) in the cochleates scales charged points, which interacted via long-range repulsive elec- linearly with the Debye length, and ranges from 13 to 35 nm for trostatic interactions and short-range attractive interactions. NaCl concentrations from 100 to 5 mM. Theoretical arguments that Planar nanoribbon to wavy ribbon with periodic undulations to include electrostatic and elastic energies explain the membrane roll- helical ribbon transitions were predicted as the range of the ing and the bilayer separation–Debye length relationship. These electrostatic interactions is increased. This study suggests a facile models suggest that the salt-induced ribbon to cochleate transi- method for accessing distinct nanoribbon architectures by vary- tion should be common to all charged bilayers possessing an ing the ionic strength (μ) of the solution because the range of intrinsic curvature, which in the present case originates from electrostatic interactions as parametrized by Debye length (λd) −1/2 molecular chirality. Our studies show how electrostatic interac- scales as μ . Recent experiments also attest that tuning the tions can be tuned to attain and control cochleate structures, which have potential for encapsulating, and releasing macromol- Significance ecules in a size-selective manner. Controlling the shape and internal architecture of assemblies of bilayer assembly | electrostatics | nanoribbon | cochleate amphiphiles is critical for many technologies. The structure, and thus the function, of these assemblies reconfigures in re- mphiphilic molecules can self-assemble into a variety of 3D, sponse to stimuli, via mechanisms that are often elusive. Here, A2D, and 1D nano- and mesoscale structures. These struc- we observe and explain how molecular reordering driven by tures serve as simplified models for understanding biological variations in electrostatic screening length induce micrometer- assemblies and their functions and have applications in drug scale structural changes in crystalline membranes of charged, delivery (1–5), regenerative medicine (6, 7), biosensing (8), hy- chiral molecules: The transformation of high aspect ratio, pla- drogen production (9, 10), and clean water technologies (11). An nar bilayers into scroll-like cochleates by increasing the solution interesting assembly structure is the nanoribbon, which is a high salt content is described and explained. Our study suggests that aspect ratio (10:1 or greater) bilayer. Nanoribbons are a gateway to this transformation should be general to charged bilayers pos- a number of other morphologies with distinct functionalities. For sessing a spontaneous curvature. example, nanoribbons of a charged chromophore amphiphile can transform to a scroll-like (cochleate) morphology when the solu- Author contributions: S.K., M.O.d.l.C., and M.J.B. designed research; C.G., S.K., D.M.V., H.L., and J.M.M. performed research; C.G., S.K., D.M.V., H.L., J.M.M., M.O.d.l.C., and M.J.B. tion ionic strength is increased (9). These cochleates serve as ef- analyzed data; and C.G., S.K., D.M.V., M.O.d.l.C., and M.J.B. wrote the paper. ficient charge-transfer agents for photocatalysts in hydrogen The authors declare no competing interest. production. Cochleate formation from liposomes of negatively charged phospholipids in the presence of multivalent cations also This article is a PNAS Direct Submission. involves a nanoribbon intermediate (3, 12, 13). Biocompatible Published under the PNAS license. phospholipid cochleates are being explored as drug-delivery agents Data deposition: All the X-ray data shown in the manuscript and the SI Appendix, as well as the code for simulating the WAXS intensity along with the data from theory calcula- because they can trap macromolecules, such as proteins, and tions, have been deposited at Bitbucket (https://bitbucket.org/NUaztec/gao_et_al_pnas_ DNA, and provide protection against degradation due to their 2019_charged_membrane/src/master/). multilayer geometry. Nanoribbons have also been observed in 1C.G. and S.K. contributed equally to this work. peptide amphiphiles (PAs), which consist of a sequence of amino 2To whom correspondence may be addressed. Email: [email protected] or acids covalently linked to an alkyl tail (14, 15). For example, a [email protected]. peptide amphiphile that stimulates collagen production has been This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10. found to self-assemble into nanotapes with an internal bilayer 1073/pnas.1913632116/-/DCSupplemental. structure (16). In a PA with alternating charged and neutral amino www.pnas.org/cgi/doi/10.1073/pnas.1913632116 PNAS Latest Articles | 1of7 Downloaded by guest on October 1, 2021 ionic strength leads to predictable changes in the nanoribbon- Fig. S1). Therefore, under the experimental conditions, nearly all related assembly morphology. For example, the period of the of the C16K1 are expected to be in their +1 ionized state. twists in amyloid fibril aggregates monotonically decreases with The AFM image of C16K1 assemblies at a silica (SiOx)/water decreasing ionic strength (21). interface (Fig. 1B) and other AFM images collected at different In this study, we analyze morphological changes in charged spots on the substrate reveal that in the absence of added salt, W planar nanoribbons as a function of increasing ionic strength. In C16K1 assembles into flat ribbons, with widths ( ) in the range L μ this regime, nanoribbon to cochleate transformations have been of a few hundred nm, lengths ( ) ranging from 2 to 20 m, and L W observed in phospholipids (12) and chromophore amphiphiles (9, aspect ratio ( / ) as high as 30. All of the ribbons exhibit the same thickness of ∼4.0 nm, as shown by a representative AFM 10, 22, 23). However, the generality and the mechanistic details of C this transition are still unknown. In particular, the correlation height scan (Fig. 1 ). This thickness is less than twice the length (5.4 nm) of fully extended C K molecules (Fig. 1A), suggesting between the ionic strength induced changes in the molecular 16 1 that the C K ribbons are bilayers with the alkyl tails of the 2 packing and the mesoscopic morphology transformations are 16 1 leaflets interdigitated. The interdigitated bilayer configuration, elusive. The principal aim of this study is to start with a nano- which has also been observed in C16K2 (24) and other PAs (17), ribbon structure and experimentally trace the micrometer to is expected for molecules with headgroup cross-sectional areas angstrom length-scale transformations in the membrane much larger than that for the alkyl tails. structure as a function of ionic strength by using a combination Screening effects are analyzed in C16K1 dispersions that contain of cryotransmission electron microscopy (cryo-TEM), liquid- NaCl at concentrations (c) ranging from 0 to 100 mM. Fig. 2 A–D atomic force microscopy (liquid-AFM),
Load more

Recommended publications
  • Development of Fractalkine-Targeted Nanofibers That Localize to Sites Of
    nanomaterials Article Development of Fractalkine-Targeted Nanofibers that Localize to Sites of Arterial Injury Hussein A. Kassam 1, David C. Gillis 1, Brooke R. Dandurand 1 , Mark R. Karver 2 , Nick D. Tsihlis 1 , Samuel I. Stupp 2,3,4,5,6 and Melina R. Kibbe 1,7,* 1 Department of Surgery, Center for Nanotechnology in Drug Delivery, University of North Carolina, Chapel Hill, NC 27599, USA; [email protected] (H.A.K.); [email protected] (D.C.G.); [email protected] (B.R.D.); [email protected] (N.D.T.) 2 Simpson Querrey Institute, Northwestern University, Chicago, IL 60611, USA; [email protected] (M.R.K.); [email protected] (S.I.S.) 3 Department of Chemistry, Northwestern University, Evanston, IL 60208, USA 4 Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA 5 Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA 6 Department of Medicine, Northwestern University, Chicago, IL 60611, USA 7 Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA * Correspondence: [email protected]; Tel.: +1-919-445-0369 Received: 30 December 2019; Accepted: 26 February 2020; Published: 28 February 2020 Abstract: Atherosclerosis is the leading cause of death and disability around the world, with current treatments limited by neointimal hyperplasia. Our goal was to synthesize, characterize, and evaluate an injectable, targeted nanomaterial that will specifically bind to the site of arterial injury. Our target protein is fractalkine, a chemokine involved in both neointimal hyperplasia and atherosclerosis. We showed increased fractalkine staining in rat carotid arteries 24 h following arterial injury and in the aorta of low-density lipoprotein receptor knockout (LDLR-/-) mice fed a high-fat diet for 16 weeks.
    [Show full text]
  • Supramolecular Design of Self-Assembling Nanofibers For
    Supramolecular design of self-assembling SPECIAL FEATURE nanofibers for cartilage regeneration Ramille N. Shaha,b, Nirav A. Shahc, Marc M. Del Rosario Limd, Caleb Hsieha,b, Gordon Nubere, and Samuel I. Stuppa,b,f,g,1 aInstitute for BioNanotechnology in Medicine, Northwestern University, 303 E. Superior Street 11th floor, Chicago, IL 60611; bDepartment of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, IL 60208; cDepartment of Orthopaedic Surgery, Northwestern University, 676 N. Saint Clair, Suite 1350, Chicago, IL 60611; dDepartment of Biomedical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208; eNorthwestern Orthopaedic Institute, 680 N. Lakeshore Dr., Suite 1028, Chicago, IL 60611; fDepartment of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208; and gDepartment of Medicine, Northwestern University, 251 East Huron Street, Suite 3-150, Chicago, IL 60611 Edited by Robert Langer, Massachusetts Institute of Technology, Cambridge, MA, and approved December 23, 2009 (received for review June 12, 2009) Molecular and supramolecular design of bioactive biomaterials planted through minimally invasive means that localizes and main- could have a significant impact on regenerative medicine. Ideal tains cells and growth factors within the defect site, promotes stem regenerative therapies should be minimally invasive, and thus cell chondrogenic differentiation, and stimulates biosynthesis. the notion of self-assembling biomaterials programmed to trans- In prior work, we developed self-assembling biomaterials form from injectable liquids to solid bioactive structures in tissue based on a broad class of molecules known as peptide amphi- is highly attractive for clinical translation. We report here on a coas- philes (PAs) that self-assemble from aqueous media into supra- sembly system of peptide amphiphile (PA) molecules designed to molecular nanofibers of high aspect ratio.
    [Show full text]
  • (12) United States Patent (10) Patent N0.: US 8,748,569 B2 Stupp Et A]
    USOO8748569B2 (12) United States Patent (10) Patent N0.: US 8,748,569 B2 Stupp et a]. (45) Date of Patent: Jun. 10, 2014 (54) PEPTIDE AMPHIPHILES AND METHODS TO Berendsen, A Glimpae of the Holy Grail?, Science, 1998, 282, pp. ELECTROSTATICALLY CONTROL 642-643.* BIOACTIVITY OF THE IKVAV PEPTIDE Voet et al, Biochemistry, John Wiley & Sons Inc., 1995, pp. 235 EPITOPE 241.* Ngo et al, Computational Complexity, Protein Structure Protection, (75) Inventors: Samuel I. Stupp, Chicago, IL (US); and the Levinthal Paradox, 1994, pp. 491-494.* Bradley et al., Limits of Cooperativity in a Structurally Modular Joshua E. Goldberger, Columbus, OH Protein: Response of the Notch Ankyrin Domain to Analogous (US); Eric J. Berns, Chicago, IL (US) Alanine Substitutions in Each Repeat, J. M01. BIoL (2002) 324, 373-386.* (73) Assignee: Northwestern University, Evanston, IL Tysseling et al., “Self-assembling peptide amphiphile promotes plas (Us) ticity of serotonergic ?bers following spinal cord injury,” J Neurosci Res, 2010, 88: 3161-3170. ( * ) Notice: Subject to any disclaimer, the term of this Tysseling-Mattiace et al., “Self-assembling nano?bers inhibit glial patent is extended or adjusted under 35 scar formation and promote axon elongation after spinal cord injury,” U.S.C. 154(b) by 0 days. JNeurosci, 2008, 28: 3814-3823. Wheeler et al., “Microcontact printing for precise control of nerve (21) Appl.No.: 13/442,210 cell growth in culture,” J Biomech Eng, 1999, 121: 73-78. Yamada et al., “Ile-Lys-Val-Ala-Val (IKVAV)-containing laminin (22) Filed: Apr. 9, 2012 alphal chain peptides form amyloid-like ?brils,” FEBS Lett, 2002, 530: 48-52.
    [Show full text]
  • 2009: Self-Assembling Peptide Amphiphile-Based Nanofiber Gel for Bioresponsive Cisplatin Delivery
    Self-assembling Peptide Amphiphile-based Nanofiber Gel for Bioresponsive Cisplatin Delivery Seongbong Jo1, Jin-Ki Kim1, Joel Anderson2, Ho-Wook Jun2, Michael A. Repka1 1 Department of Pharmaceutics, School of Pharmacy, The University of Mississippi, 2 Department of Biomedical Engineering, University of Alabama at Birmingham Statement of Purpose: Peptide amphiphiles (PAs) 8.5-fold greater than that with MR = 1, respectively. TEM composed of a hydrophilic biomimetic peptide sequence images exhibited that the CDDP/PA gels were composed and a hydrophobic alkyl chain have been extensively of the nanofibers of 8-10 nm in diameter and several studied as biomaterials which mimic extracellular micrometers in length. In addition, physical crosslinking matrices [1-3]. Although in situ formed PA gels have of the self-assembled nanofibers was pronounced in the been intensively examined for cellular engineering, their PA gel (Fig. 1A). applications for drug delivery have been limited thus far. The aim of this study is to develop a bioresponsive cisplatin (CDDP) delivery system with a biomimetic peptide amphiphile (PA). The self-assembling PA comprised of RGDS, MMP-2-sensitive GTAGLIGQ and a fatty acid has been investigated for spontaneous gel formation via complexation with CDDP. MMP-sensitive CDDP release from the PA gel has been examined to simulate tumor-responsive CDDP release in vitro. Methods: A peptide consisting of RGDS and MMP- sensitive GTAGLIGQ was synthesized by standard Fmoc- (A) (B) chemistry. PA was successfully synthesized by a coupling Figure 1. TEM images of nanofiber-networked CDDP-PA gels reaction to acylate the N-terminus of the peptide with with MR = 1.5 at preparation (A) and after enzymatic palmitic acid.
    [Show full text]
  • The Osteogenic Differentiation Effect of the FN Type 10-Peptide Amphiphile on PCL Fiber
    International Journal of Molecular Sciences Article The Osteogenic Differentiation Effect of the FN Type 10-Peptide Amphiphile on PCL Fiber Ye-Rang Yun 1, Hae-Won Kim 2,3,4,* and Jun-Hyeog Jang 5,* ID 1 Industrial Technology Research Group, Research and Development Division, World Institute of Kimchi, Nam-Gu, Gwangju 61755, Korea; [email protected] 2 Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, Korea 3 Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Korea 4 Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, Korea 5 Department of Biochemistry, Inha University School of Medicine, Incheon 22212, Korea * Correspondence: [email protected] (H.-W.K.); [email protected] (J.-H.J.); Tel.: +82-41-550-3081 (H.-W.K.); +82-32-890-0930 (J.-H.J.); Fax: +82-41-550-3085 (H.-W.K.); +82-32-882-1877 (J.-H.J.) Received: 1 November 2017; Accepted: 3 January 2018; Published: 4 January 2018 Abstract: The fibronectin type 10-peptide amphiphile (FNIII10-PA) was previously genetically engineered and showed osteogenic differentiation activity on rat bone marrow stem cells (rBMSCs). In this study, we investigated whether FNIII10-PA demonstrated cellular activity on polycaprolactone (PCL) fibers. FNIII10-PA significantly increased protein production and cell adhesion activity on PCL fibers in a dose-dependent manner. In cell proliferation results, there was no effect on cell proliferation activity by FNIII10-PA; however, FNIII10-PA induced the osteogenic differentiation of MC3T3-E1 cells via upregulation of bone sialoprotein (BSP), collagen type I (Col I), osteocalcin (OC), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) mitochondrial RNA (mRNA) levels; it did not increase the alkaline phosphatase (ALP) mRNA level.
    [Show full text]
  • Self-Assembling Peptides and Their Application in the Treatment of Diseases
    International Journal of Molecular Sciences Review Self-Assembling Peptides and Their Application in the Treatment of Diseases Sungeun Lee 1, Trang H.T. Trinh 1, Miryeong Yoo 1, Junwu Shin 1, Hakmin Lee 1, Jaehyeon Kim 1, Euimin Hwang 2, Yong-beom Lim 2 and Chongsuk Ryou 1,* 1 Department of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Gyeonggi-do 15588, Korea; [email protected] (S.L.); [email protected] (T.H.T.T.); [email protected] (M.Y.); [email protected] (J.S.); [email protected] (H.L.); [email protected] (J.K.) 2 Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Korea; [email protected] (E.H.); [email protected] (Y.-b.L.) * Correspondence: [email protected]; Tel.: +82-31-400-5811; Fax: +82-31-400-5958 Received: 30 September 2019; Accepted: 20 November 2019; Published: 21 November 2019 Abstract: Self-assembling peptides are biomedical materials with unique structures that are formed in response to various environmental conditions. Governed by their physicochemical characteristics, the peptides can form a variety of structures with greater reactivity than conventional non-biological materials. The structural divergence of self-assembling peptides allows for various functional possibilities; when assembled, they can be used as scaffolds for cell and tissue regeneration, and vehicles for drug delivery, conferring controlled release, stability, and targeting, and avoiding side effects of drugs. These peptides can also be used as drugs themselves. In this review, we describe the basic structure and characteristics of self-assembling peptides and the various factors that affect the formation of peptide-based structures.
    [Show full text]
  • Design of 18 Nm Doxorubicin-Loaded 3-Helix Micelles: Cellular Uptake and Cytotoxicity in Patient-Derived GBM6 Cells
    bioRxiv preprint doi: https://doi.org/10.1101/2020.05.04.072892; this version posted May 5, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Design of 18 nm Doxorubicin-loaded 3-helix Micelles: Cellular Uptake and Cytotoxicity in Patient-derived GBM6 Cells Benson T. Jung1, Katherine Jung2, Marc Lim3, Michael Li2, Raquel Santos4, Tomoko Ozawa4, Ting Xu*1, 2, 5 1Department of Materials Science and Engineering, University of California, Berkeley, CA, United States 2Department of Chemistry, University of California, Berkeley, CA, United States 3UCB-UCSF Graduate Program in Bioengineering, University of California, Berkeley, CA, United States 4Department of Neurological Surgery, University of California, San Francisco, CA, United States 5Material Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States * Mailing address: 381 Hearst Memorial Mining Building, UC Berkeley, Berkeley, CA 94720. Email: [email protected] Keywords: peptide amphiphiles, cellular uptake, patient-derived xenograft, glioblastoma, nano- bio interface, PEGylation 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.05.04.072892; this version posted May 5, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Abstract: The fate of nanocarrier materials at the cellular level constitutes a critical checkpoint in the development of effective nanomedicines, determining whether tissue level accumulation results in therapeutic benefit.
    [Show full text]
  • Bioinspired Kokkoli Paper
    Engineering Biomimetic Peptides for Targeted Drug Delivery EFROSINI KOKKOLI University of Minnesota, Minneapolis Targeted drug delivery, the ability to target a drug to a specific site of disease, is the leading frontier in the pursuit of better strategies that will allow us to selectively treat diseases with minimal side effects, and peptide functionalized nanovectors are a promising class of targeted delivery vehicles. Biomimetic peptide targeting ligands, peptides that mimic cell binding domains of proteins, can be readily designed to bind a target (for example, an adhesion receptor on the surface of a cell) selectively with high affinity and specificity, and more importantly are molecules accessible by chemical synthesis and relatively compact compared to antibodies and full proteins. PEPTIDE FUNCTIONALIZED LIPOSOMES Liposomes are the most extensively studied drug transport systems to date, with a number of non-targeted liposome delivery systems already FDA approved and being used in a clinical setting. Liposomes can range from approximately 50 nm to microns in diameter, although diameters 100-200 nm are often desirable for drug delivery applications. “Stealth” liposomes, also often referred to as sterically stabilized liposomes, have short polyethylene glycol (PEG) polymer strands attached to a fraction of the hydrophilic lipid headgroups. These PEG chains form a polymer brush on the surface of the liposome that, through steric repulsion, resist protein adhesion and therefore clearance by the reticuloendothelial system (RES). Today, one ongoing area of effort in liposome drug delivery research involves conjugating ligands, such as peptides, onto “stealth” 1 liposomes to confer active as well as passive targeting to these drug carriers.
    [Show full text]
  • Selective Differentiation of Neural Progenitor Cells by High–Epitope
    R EPORTS sequence glutamic acid–glutamine–serine (EQS) (36). As discussed below, these mole- Selective Differentiation of cules form physically similar scaffolds by self- assembly, but cells encapsulated within the Neural Progenitor Cells by EQS gels did not sprout neurites or differentiate morphologically or histologically. High–Epitope Density Nanofibers The chemical structure of the IKVAV- containing peptide amphiphile (IKVAV- Gabriel A. Silva,1*† Catherine Czeisler,2* Krista L. Niece,3 PA) and a molecular graphics illustration of Elia Beniash,3 Daniel A. Harrington,3 John A. Kessler,2 its self-assembly are shown in Fig. 1A, and Samuel I. Stupp1,3,4‡ a scanning electron micrograph of the scaf- fold it forms is shown in Fig. 1B. In addi- Neural progenitor cells were encapsulated in vitro within a three-dimensional tion to the neurite-sprouting epitope, the network of nanofibers formed by self-assembly of peptide amphiphile mole- molecules contain a Glu residue that gives cules. The self-assembly is triggered by mixing cell suspensions in media with them a net negative charge at pH 7.4 so that dilute aqueous solutions of the molecules, and cells survive the growth of the cations in the cell culture medium can nanofibers around them. These nanofibers were designed to present to cells the screen electrostatic repulsion among them neurite-promoting laminin epitope IKVAV at nearly van der Waals density. and promote self-assembly when cell sus- Relative to laminin or soluble peptide, the artificial nanofiber scaffold induced pensions are added. The rest of the se- very rapid differentiation of cells into neurons, while discouraging the devel- quence consists of four Ala and three Gly opment of astrocytes.
    [Show full text]
  • Enhancing Peptide Biomaterials for Biofabrication
    polymers Review Enhancing Peptide Biomaterials for Biofabrication Kate Firipis 1,2 , David R. Nisbet 3,4,5, Stephanie J. Franks 3 , Robert M. I. Kapsa 1,2,6, Elena Pirogova 1,2 , Richard J. Williams 1,7,*,† and Anita Quigley 1,2,6,*,† 1 Biofab3D, Aikenhead Centre for Medical Discovery, St Vincent’s Hospital Melbourne, Fitzroy, VIC 3065, Australia; kate.fi[email protected] (K.F.); [email protected] (R.M.I.K.); [email protected] (E.P.) 2 Biomedical and Electrical Engineering, School of Engineering, RMIT University, Melbourne, VIC 3000, Australia 3 Laboratory of Advanced Biomaterials, The Australian National University, Acton, Canberra, ACT 2601, Australia; [email protected] (D.R.N.); [email protected] (S.J.F.) 4 The Graeme Clark Institute, Faculty of Engineering and Information Technology, Melbourne, VIC 3000, Australia 5 Faculty of Medicine, Dentistry and Health Services, The University of Melbourne, Melbourne, VIC 3000, Australia 6 Department of Medicine, Melbourne University, St Vincent’s Hospital Melbourne, Fitzroy, VIC 3064, Australia 7 Institute of Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Waurn Ponds, VIC 3216, Australia * Correspondence: [email protected] (R.J.W.); [email protected] (A.Q.) † These authors have equal last authorship. Abstract: Biofabrication using well-matched cell/materials systems provides unprecedented oppor- tunities for dealing with human health issues where disease or injury overtake the body’s native regenerative abilities. Such opportunities can be enhanced through the development of biomaterials with cues that appropriately influence embedded cells into forming functional tissues and organs.
    [Show full text]
  • 2020-AIME-HAL-Multivalent Clus
    Multivalent Clustering of Adhesion Ligands in Nanofiber-Nanoparticle Composites Dounia Dems, Ronit Freeman, Kyle Riker, Thibaud Coradin, Samuel Stupp, Carole Aimé To cite this version: Dounia Dems, Ronit Freeman, Kyle Riker, Thibaud Coradin, Samuel Stupp, et al.. Multivalent Clustering of Adhesion Ligands in Nanofiber-Nanoparticle Composites. Acta Biomaterialia, Elsevier, 2021, 119, pp.303-311. 10.1016/j.actbio.2020.11.009. hal-03019265 HAL Id: hal-03019265 https://hal.archives-ouvertes.fr/hal-03019265 Submitted on 23 Nov 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Multivalent Clustering of Adhesion Ligands in Nanofiber-Nanoparticle Composites Dounia Dems,§‡ Ronit Freeman,ǂ‡^ Kyle D. Riker,^ Thibaud Coradin,§ Samuel I. Stupp,ǂ⊥∥Δ# Carole Aim駆* § Sorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris, 4 place Jussieu, 75252 Paris cedex 05, France ǂ Simpson Querrey Institute, Northwestern University, 303 East Superior Street, Chicago, Illinois 60611, USA. ⊥ Department of Materials and Science & Engineering, Δ Department of Chemistry, and ∥ Department of Biomedical Engineering, Northwestern University, 2220 Campus Drive, Evanston, Illinois 60208, United States # Department of Medicine, Northwestern University, 676 North St. Clair Street, Chicago, Illinois 60611, United States ^ Department of Applied Physical Sciences, University of North Carolina, 121 South Rd, Chapel Hill, North Carolina, 27514, United States.
    [Show full text]
  • Peptide Amphiphiles in Corneal Tissue Engineering
    J. Funct. Biomater. 2015, 6, 687-707; doi:10.3390/jfb6030687 OPEN ACCESS Journal of Functional Biomaterials ISSN 2079-4983 www.mdpi.com/journal/jfb Review Peptide Amphiphiles in Corneal Tissue Engineering Martina Miotto, Ricardo M. Gouveia and Che J. Connon * Institute of Genetic Medicine, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK; E-Mails: [email protected] (M.M.); [email protected] (R.M.G.) * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +44-191-241-8623. Academic Editor: Dimitrios Karamichos Received: 30 June 2015 / Accepted: 3 August 2015 / Published: 6 August 2015 Abstract: The increasing interest in effort towards creating alternative therapies have led to exciting breakthroughs in the attempt to bio-fabricate and engineer live tissues. This has been particularly evident in the development of new approaches applied to reconstruct corneal tissue. The need for tissue-engineered corneas is largely a response to the shortage of donor tissue and the lack of suitable alternative biological scaffolds preventing the treatment of millions of blind people worldwide. This review is focused on recent developments in corneal tissue engineering, specifically on the use of self-assembling peptide amphiphiles for this purpose. Recently, peptide amphiphiles have generated great interest as therapeutic molecules, both in vitro and in vivo. Here we introduce this rapidly developing field, and examine innovative applications of peptide amphiphiles to create natural bio-prosthetic corneal tissue in vitro. The advantages of peptide amphiphiles over other biomaterials, namely their wide range of functions and applications, versatility, and transferability are also discussed to better understand how these fascinating molecules can help solve current challenges in corneal regeneration.
    [Show full text]