Recombinant Human FLT3 Protein

Leader in Biomolecular Solutions for Life Science Recombinant Human FLT3 Protein

Catalog No.: RP02230 Recombinant

Sequence Information Background

Species Gene ID Swiss Prot Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver Human 2322 P36888 kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that Tags acts as cell-surface receptor for the cytokine FLT3LG and regulates diﬀerentiation, C-Fc & His proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream eﬀector MTOR. Promotes activation of RAS signaling and phosphorylation of Synonyms downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause Flt-3; Flk-2; STK-1; CD135; FLK2; FLT-3 constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Basic Information

Product Information Description Recombinant Human FLT3 Protein is produced by HEK293 cells expression system. Source Puriﬁcation The target protein is expressed with sequence (FLT3(Asn27-Ser543)&hFc(Glu99- HEK293 cells > 95% by SDS- Ala330)) of human FLT3 (Accession #) fused with a Fc and His Tag at the C-terminal. . PAGE. Bio-Activity Endotoxin ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human FLT3LG, mFc-His tagged protein can bind Human FLT3, hFc-His tagged protein in a linear range of 0.128-10.02 Formulation ng/ml. Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 14 % trehalose is added Storage as protectants before lyophilization. Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for Reconstitution up to 1 week. Reconstitute with deionized water Avoid repeated freeze/thaw cycles.

Contact

400-999-6126

[email protected]

www.abclonal.com.cn Validation Data

Recombinant Human FLT3 Protein was ELISA plate pre-coated by 2 μg/ml (100 determined by SDS-PAGE with Coomassie μl/well) Human FLT3LG, mFc-His tagged Blue, showing bands at 125-130 kDa protein can bind Human FLT3, hFc-His tagged protein in a linear range of 0.128-10.02 ng/ml.