RESEARCH ARTICLE A high throughput, functional screen of human Body Mass Index GWAS loci using tissue-specific RNAi Drosophila melanogaster crosses Thomas J. Baranski1*, Aldi T. Kraja2, Jill L. Fink1, Mary Feitosa2, Petra A. Lenzini2, Ingrid B. Borecki3, Ching-Ti Liu4, L. Adrienne Cupples4, Kari E. North5, Michael A. Province2* a1111111111 1 Department of Internal Medicine, Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, St. Louis, Missouri, United States of America, 2 Department of Genetics and a1111111111 Center for Genome Sciences and Systems Biology, Division of Statistical Genomics, Washington University a1111111111 School of Medicine, St. Louis, Missouri, United States of America, 3 Department of Biostatistics, University of a1111111111 Washington, Seattle, Washington, United States of America, 4 Department of Biostatistics, Boston University a1111111111 School of Public Health, Boston, Massachusetts, United States of America, 5 Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina, United States of America *
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[email protected] (MAP) OPEN ACCESS Citation: Baranski TJ, Kraja AT, Fink JL, Feitosa M, Abstract Lenzini PA, Borecki IB, et al. (2018) A high Human GWAS of obesity have been successful in identifying loci associated with adiposity, throughput, functional screen of human Body Mass Index GWAS loci using tissue-specific RNAi but for the most part, these are non-coding SNPs whose function, or even whose gene of Drosophila melanogaster crosses. PLoS Genet 14 action, is unknown. To help identify the genes on which these human BMI loci may be oper- (4): e1007222. https://doi.org/10.1371/journal. ating, we conducted a high throughput screen in Drosophila melanogaster.