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Quality Properties of Beef Jerky Cured with Salted- Fermented Anchovy and Shrimp

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Quality Properties of Beef Jerky Cured with Salted- Fermented Anchovy and Shrimp QUALITY PROPERTIES OF BEEF JERKY CURED WITH SALTED- FERMENTED ANCHOVY AND SHRIMP Hyeon-Woong Yum1, Jin-Kyu Seo1 and Han-Sul Yang1,2* 1Division of Applied Life Science Graduate School (BK21 plus), Gyeongsang National University, Jinju, 52828, Korea 2 Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Korea *Corresponding author email: [email protected] Abstract –The aim of this study is to evaluate the processed products due to their salty, unique availability of salted fermented anchovy (SFA) flavor and texture (Kim et al., 2011). including salted and shrimp (SFS) as a marinade Therefore, the beef jerky was prepared by curing of beef jerky. The quality properties of beef jerky with SFF made of anchovy and shrimp, and the including proximate composition, pH, color, shear quality properties of beef jerky were force, SDS-PAGE and sensory evaluation were investigated. Higher moisture content was found investigated. in the beef jerky cured with SFAs and SFSs than the control (p<0.05). SDS-PAGE result no significant differences among the treatment. The II. MATERIALS AND METHODS SFAs and SFSs had the effect of causing a decrease in shear force (p<0.05). The SFA and 1. Preparation of SFF and beef jerky SFS also had the effect of decreasing the lightness SFF products were purchased from local market. value. Color score of sensory evaluations were Anchovy and shrimp were washed thoroughly increased by addition of SFA and SFS. Therefore, and mixed with salt as a ratio of 20% to raw fish. we conclude the SFA and SFS can improve the They were then fermented at room temperature quality properties of the beef jerky and SFA and SFS a good ingredient for the curing solution. for three months. The salinity of the SFF juice was measured using aconductivity meter and Key Words – beef jerky, salt-fermented anchovy, adjusted to 11.5% salt using distilled water. shrimp, quality property Beef semimembranosus muscles were obtained at 48 h post-slaughter from a local market on three different processing days. All I. INTRODUCTION subcutaneous and intermuscular fat and visible connective tissue were removed from the fresh Jerky is old meat products and has been sold for muscles. The sample was sliced to 5 mm thick many years in the form of snack foods. Jerky is pieces with a meat slicer. All slices samples obtained from obtained from sliced whole were divided randomly into three groups muscles marinated and dried. Jerky products are (Control, SFA, and SFS) and cured with characterized by a diversity of raw materials, different curing solutions in a cold room for 24 h. spices, and other additives, and by the The salt-water was prepared by dissolving technological procedures such as curing and sodium chloride in distilled water and its salinity drying (Konieczny et al., 2007). adjusted to 11.5% salt. The formulation of cure Salt is one of the most frequently used solution is presented in Table 1. ingredients in meat processing. Salt affects the After curing, all samples were dried using a dry- flavor, texture and self-life of meat products oven (DS80-1, Dasol Scientific Co. Ltd., Korea) (Gillette, 1985). However, an increased effect at a temperature of 70oC during 8 h to achieve a has been made to reduce the amount of salt in water activity of 0.82. After drying, all samples foodstuffs (Costa-Corredor et al., 2009). Salt- were cooling at a room temperature during 12 h. fermented fish (SFF) product was added salt and After cooling at a room temperature, proximate fermentation in muscles and viscera of fishes composition (%), pH, color, shear force (kg/cm2), and shellfishes. Also, SFF and their liquid sauce have been widely utilized in a variety of 62nd International Congress of Meat Science and Technology, 14-19th August 2016, Bangkok, Thailand SDS-PAGE and sensory evaluations were superscripts are significantly different (p<0.05). 1) analyzed. Treatments are the same as Table 1. 2. Statistical analyzed 2. pH, color and shear force Data was analyzed by the procedures of The results for pH, lightness and shear force of generalized linear model. Duncan’s multiple the beef jerky are shown in Table 3. The pH range test was employed to determine the values of jerky generally range from 5.75 to 5.88. significance between treatments (SAS 9.3, 2014). All The pH value of beef jerky cured by SFA and data were presented by standard error and SFS were higher than the control jerky (p<0.05). significance level of p<0.05 was used for Kim et al. (2014) reported that beef jerky statistical analysis of means from treatments. containing the SFA and SFS has a significantly higher pH value than the salt solution. Table 1. Formulation of the curing solution for Color results indicated that the lightness value making beef jerky was significantly lower in the SFS than the other Treatments1) samples (p<0.05). The lightness value was lower Ingredients (%) C T1 T2 in the cured SFA and SFS of beef jerky than the Salt-water 9.0 - - control jerky. According to Konieczny et al. SFA-water2) - 9.0 - (2007), color of beef jerky can change according SFS-water3) - - 9.0 to the drying time. However, in the present study, Sugar 3.0 3.0 3.0 because the same drying conditions were applied Starch syrup 2.5 2.5 2.5 to the treatments, the effect of color change was Black pepper 0.2 0.2 0.2 caused by SFA and SFS. Shear force were significant differences among Sodium nitrite 0.024 0.024 0.024 the control and treatments (p<0.05). The shear Total 14.724 14.724 14.724 force of beef jerky cured by SFA and SFS were 1)Control: salt marinated, T1: salt-fermented anchovy (SFA) marinated, T2: salt-fermented shrimp (SFS) lower than the control jerky (p<0.05). These marinated. results agree with the previous report that shear force is an affected by moisture content (Yang et al., 2012) and moisture content increased by salt III. RESULTS AND DISCUSSION concentration (Monin et al., 1997). 1. Proximate composition (%) Table 3. pH, lightness and shear force of beef jerky Proximate composition of results presented in cured with salted and fermented anchovy and shrimp Treat Lightness Shear force Table 2. The moisture content, crude fat and pH ments1) (L*) (kg/cm2) crude protein of beef jerky were significant B A differences in the treatments (p<0.05). SFA and C 5.75±0.01 28.61±1.81 4.52±1.03A A AB SFS were the lower in protein and fat content. T1 5.88±0.06 26.74±1.60 3.47±0.95B However, the moisture content of beef jerky A B cured by SFA and SFS were higher than the T2 5.88±0.01 25.14±2.71 3.48±0.93B control jerky. A-BMeans±SD in the same column with different superscripts are significantly different (p<0.05). 1) Table 2. Proximate composition (%) of beef jerky Treatments are the same as Table 1. cured with salted and fermented anchovy and shrimp Treat Crude Crude 3. Sensory evaluation of beef jerky Moisture Ash ments1) fat protein As presented in Table 4, flavor and texture were 30.49± 16.24± 60.52± 2.13± significantly different between treatments in C B A A 0.41 2.66 0.04 0.07 sensory evaluation (p<0.05). The texture of beef 34.69± 14.19± 57.29± 2.03± T1 A B B jerky showed a similar trend with the result of 0.07 1.69 0.72 0.11 shear force. Flavor values for treatments cured 34.06± 14.38± 57.84± 2.08± T2 with SFA and SFS were higher than that for 0.37A 1.63B 0.60B 0.03 control. The SFA and SFS contain various A-BMeans±SD in the same column with different 62nd International Congress of Meat Science and Technology, 14-19th August 2016, Bangkok, Thailand compounds, because the fish fermented process induces the transformation of organic substances IV. CONCLUSION to various simpler compounds, which are the origins of their unique flavors and aroma The beef jerky cured with SFA and SFS (Peralta et al., 2008). exhibited good moisture content. Also, the SFA and SFS improved the texture and color of the Table 4. Sensory evaluation of beef jerky cured with beef jerky. The compounds from SFA and SFS salted and fermented anchovy and shrimp could be good enhancers for the flavor of the Treat Off Accept Color Flavor Texture beef jerky. ments1) flavor ability 7.29± 4.57± 1.86± 5.29±0. 7.14± C B B 0.49 1.13 0.69 53 0.49 ACKNOWLEDGEMENTS 7.43± 7.14± 1.71± 6.47±0. 7.43± T1 0.79 0.69A 0.76 45A 0.53 This research was supported (Project No. 315014-05- 7.43± 7.14± 1.86± 6.48±0. 7.71± T2 1-SB150) by Korea Institute of Planning and 0.53 0.90A 0.90 43A 0.69 Evaluation for Technology in Food, Agriculture, A-BMeans±SD in the same column with different Forestry and Fisheries. Also, H.W. Yum and J.K. Seo superscripts are significantly different (p<0.05). 1)Treatments are the same as Table 1. were supported by the Brain Korea 21 plus project from the Ministry of Education and Human 4. SDS-PAGE of beef jerky Resources Development, Republic of Korea.` The Figure 1 was shown SDS-PAGE gel patterns of myofibril of beef jerky treated REFERENCES with salt agent.
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