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Supplementary Material Supplementary Material Table S1 The sequence amount of different types of saprotroph fungi from different landfill samples, classified by FUNGuild Undefined Wood Soil Plant Leaf Dung Litter Samples Saprotroph Saprotroph Saprotroph Saprotroph Saprotroph Saprotroph Saprotroph A03 4766 13 7 2 0 2 0 A06 2627 261 16 3 1 2 2 A09 644 125 5 1 0 2 0 A12 2875 47 9 3 0 2 0 A15 5114 544 78 3 0 4 0 B03 3696 585 5 3 0 2 11 B06 2116 1452 46 4 1 3 33 B09 402 1602 24 1 0 1 0 B12 6476 503 9 2 487 2 6 B15 1267 128 4 2 0 3 0 C03 2475 1186 220 3 0 392 0 C06 3674 68 12 3 0 3 0 C09 3632 191 1095 191 0 134 0 C12 5004 195 6 0 0 1 0 C15 6292 972 424 52 0 30 1 Notes: The OTU numbers of undefined saprotroph, wood saprotroph, soil saprotroph, plant saprotroph, leaf saprotroph, dung saprotroph and litter saprotroph detected in all samples are 105, 24, 3, 6, 2, 4, and 4 respectively S1 Table S2 Functional information of top 20 genera* of fungi in landfill Genera Taxon Taxon Level Trophic Mode Guild Confidence Ranking f_Hypocreaceae_unclassified Hypocreales order Saprotroph Undefined Saprotroph Possible Fusarium Fusarium genus Pathotroph- Animal Pathogen-Endophyte-Lichen Possible Saprotroph- Parasite-Plant Pathogen-Soil Symbiotroph Saprotroph-Wood Saprotroph Aspergillus Eurotiales order Saprotroph Undefined Saprotroph Possible Aspergillus niger species Pathotroph-Saprotroph Plant Pathogen-Wood Saprotroph Probable Aspergillus flavus species Pathotroph-Saprotroph Animal Pathogen-Plant Pathogen- Probable Wood Saprotroph Aspergillus terreus species Pathotroph-Saprotroph Animal Pathogen-Wood Saprotroph Probable Aspergillus ustus species Saprotroph Wood Saprotroph Highly probable Aspergillus aculeatus species Pathotroph Plant Pathogen Probable Talaromyces Talaromyces genus Saprotroph Undefined Saprotroph Probable Gibberella Gibberella genus Pathotroph Plant Pathogen Probable Penicillium Eurotiales order Saprotroph Undefined Saprotroph Possible Penicillium digitatum species Pathotroph Plant Pathogen Probable Penicillium oxalicum species Pathotroph Plant Pathogen Probable Penicillium purpurogenum species Pathotroph Plant Pathogen Probable Penicillium citrinum species Pathotroph Plant Pathogen Probable Malassezia Malassezia genus Pathotroph-Saprotroph Animal Pathogen-Undefined Probable Saprotroph Meyerozyma Saccharomycetales order Saprotroph Undefined Saprotroph Possible g_unclassified_Ustilaginaceae Ustilaginaceae family Pathotroph Plant Pathogen Probable S2 Trichoderma Hypocreales order Saprotroph Undefined Saprotroph Possible Trichoderma longibrachiatum species Pathotroph- Endophyte-Plant Pathogen Probable Symbiotroph Pseudallescheria Pseudallescheria genus Saprotroph Undefined Saprotroph Probable Trichothecium Trichothecium genus Pathotroph Plant Pathogen Probable Pseudeurotium Pseudeurotium genus Saprotroph Undefined Saprotroph Probable Guehomyces Guehomyces genus Saprotroph Undefined Saprotroph Probable Candida Saccharomycetales order Saprotroph Undefined Saprotroph Possible Cladosporium Cladosporium species Symbiotroph Endophyte Highly probable sphaerospermum Cladosporium genus Pathotroph- Animal Pathogen-Endophyte-Lichen Possible Saprotroph- Parasite-Plant Pathogen-Wood Symbiotroph Saprotroph g__unclassified_Pezizaceae Peziza genus Saprotroph- Dung Saprotroph-Ectomycorrhizal- Possible Symbiotroph Litter Saprotroph-Undefined Saprotroph Note: *There is no functional information of p_Ascomycota_unclassified, g_unclassified_Fungi and g_unclassified_Ustilaginaceae. S3 Fig. S1 Sampling sites (A, B and C) and their horizontal distance Fig. S2 Spearman correlation analysis among the properties of refuse and leachate samples, including landfilled age and physiochemical parameters (pH, DOC, VS, ammonia, nitrite, and nitrate). S means solid samples, i.e. refuse; L means leachate samples S4 60 A B C 50 h h 40 g fg efg cde ef def 30 bc bc bcd VS Content (%) ab ab ab 20 a 10 3m 6m 9m 12m 15m Depth Fig. S3 Volatile solid (VS) content variation of refuse samples (A, B, C) from different depth in landfill. Small letters above bars indicate significant difference among VS values observed in different refuse samples A03 A03L 250 A06 A06L A09 A09L A12 A12L A15 A15L 200 B03 B06 B09 B12 150 B15 C03 C03L C06 C06L C09 C09L 100 OTU number C12 C12L C15 C15L 50 0 0 5000 10000 15000 20000 Sequence amount Fig. S4 Rarefaction curve of observed fungal OTUs to assess sequencing depth S5 1.0 0.5 Relative Abundance 0.0 A03 A06 A09 A12 A15 A03L A06L A09L Others 1.0 A12L A15L Zygomycota 0.9 B03 Basidiomycota Ascomycota SampleB06 0.8 B09 B12 0.7 B15 C03 0.6 C06 (a) C09 0.5 C12 C15 0.4 C03L C06L 0.3 C09L Relative Abundance C12L 0.2 C15L 0.1 0.0 A03 A06 A09 A12 A15 A03L Others A06L Wallemiomycetes A09L Ustilaginomycetes A12L Tremellomycetes A15L Agaricomycetes Sordariomycetes B03 B06 Saccharomycetes SampleB09 Pezizomycetes B12 Orbiliomycetes B15 Leotiomycetes C03 Eurotiomycetes C06 Dothideomycetes C09 C12 (b) C15 C03L C06L C09L C12L C15L S6 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 Relative Abundance 0.2 0.1 0.0 Fig. S5 Relative abundance of differentA03 phyla (a), landfill samples A06 A09 A12 A15 A03L Others A06L Ustilaginaceae A09L Cystofilobasidiaceae A12L Microascaceae A15L Nectriaceae B03 Hypocreaceae B06 Debaryomycetaceae SampleB09 Pezizaceae B12 Pseudeurotiaceae B15 Davidiellaceae C03 Trichocomaceae C06 C09 C12 (c) C15 classes (b) and families (c) among fungalC03L communities from C06L C09L C12L C15L S7 .
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