applied sciences Article A Comprehensive HPLC-DAD-ESI-MS Validated Method for the Quantification of 16 Phytoestrogens in Food, Serum and Urine Susana Alejandra Palma-Duran 1,2, Graciela Caire-Juvera 3, Melissa María Campa-Siqueiros 3, Karina María Chávez-Suárez 3, María del Refugio Robles-Burgueño 2, María Lourdes Gutiérrez-Coronado 2, María del Carmen Bermúdez-Almada 2, María del Socorro Saucedo-Tamayo 3, Patricia Grajeda-Cota 1 and Ana Isabel Valenzuela-Quintanar 2,* 1 Biomolecular Medicine, Department of Metabolism, Division of Systems Medicine, Digestion, and Reproduction, Imperial College London, London SW7 2AZ, UK;
[email protected] (S.A.P.-D.);
[email protected] (P.G.-C.) 2 Department of Food Science, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo 83004, Mexico;
[email protected] (M.d.R.R.-B.);
[email protected] (M.L.G.-C.);
[email protected] (M.d.C.B.-A.) 3 Department of Nutrition, Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo 83004, Mexico;
[email protected] (G.C.-J.);
[email protected] (M.M.C.-S.);
[email protected] (K.M.C.-S.);
[email protected] (M.d.S.S.-T.) * Correspondence:
[email protected]; Tel.: +52-(662)-2892400 Received: 8 October 2020; Accepted: 12 November 2020; Published: 17 November 2020 Abstract: There has been increased interest in phytoestrogens due to their potential effect in reducing the risk of developing cancer and cardiovascular disease. To evaluate phytoestrogens’ exposure, sensitive and accurate methods should be developed for their quantification in food and human matrices. The present study aimed to validate a comprehensive liquid chromatography-mass spectrometry (LC-MS) method for the quantification of 16 phytoestrogens: Biochanin A, secoisolariciresinol, matairesinol, enterodiol, enterolactone, equol, quercetin, genistein, glycitein, luteolin, naringenin, kaempferol, formononetin, daidzein, resveratrol and coumestrol, in food, serum and urine.