Mycoscience 59 (2018) 49e53

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Mycoscience

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Short communication Coltriciella minuscula sp. nov., a new species of poroid on Pinus merkusii from an Indonesian tropical forest

* Dewi Susan a, Atik Retnowati b, Nampiah Sukarno c, a Graduate School of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Darmaga, Bogor 16680, Indonesia b Herbarium Bogoriense, Botany Division, Research Center for Biology, Indonesian Institute of Sciences, Jl. Raya Jakarta-Bogor KM 46 Cibinong, Bogor 16911, Indonesia c Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Kampus IPB Darmaga, Bogor 16680, Indonesia article info abstract

Article history: Coltriciella minuscula sp. nov. is described and illustrated from a specimen collected on Pinus merkusii Received 27 September 2016 from Bogor, Indonesia. This species is characterized by the size of its basidiocarp up to 4 mm in diam, Received in revised form with long hair on the stipe and with ornamented spores. Both morphological distinctiveness and 5 August 2017 phylogenetic separation based upon analyses of nrDNA ITS sequences support the establishment of this Accepted 9 August 2017 new species. Morphological dissimilarities between C. minuscula and closely related species are Available online 7 December 2017 discussed. © 2017 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. Keywords: Phylogeny Taxonomy

The genus Coltriciella was erected by Murrill (1904) as a Ryvarden, 2004). Formation of ectomycorrhizal associations is monotypic genus typified by C. dependens (Berk. & M.A. Curtis) also known (Tedersoo, Suvi, Beaver, & Koljalg,~ 2007a; Tedersoo, Murrill to accommodate species similar to Coltricia Gray but which Suvi, Beaver, & Saar, 2007b). are epixylous and have a vertically-attached pileus. The generic Coltriciella and Coltricia share similar morphological features, concept was broadened by the discovery of species which form but the latter differs in having smooth basidiospores (Dai, 2010; basidiocarps varying from resupinate, effuse-reflexed, pendent or Ryvarden, 1991, 2004). Phylogenetically, Coltriciella and Coltricia stipitate with a monomitic hyphal system, blackish in KOH, colored comprise a monophyletic clade (Tedersoo et al., 2007b; Wagner & and ornamented basidiospores (Corner, 1991; Ryvarden, 2004). Fischer, 2002), but Larsson et al. (2006) has contended that Based on morphological characteristics, Coltriciella was formerly phylogenetic analysis does not support a separation of the two accommodated in the family (Dai, 2010; genera. Ryvarden, 2004; Ryvarden & Johansen, 1980). However, recent No species of Coltriciella has previously been reported from molecular phylogenetic evidence indicates that the genus is distinct Indonesia, but during a recent study of poroid fungi from West Java, from Hymenochaetaceae, as is the genus Coltricia. Together, these a specimen of Coltriciella was collected from Pinus merkusii Jungh. & two genera form a joint Coltricia subclade (Larsson et al., 2006; de Vriese. Due to its morphological distinction from other members Wagner & Fischer, 2002). Coltriciella comprises 13 species from of the genus, we describe this as a new species. This is also sup- tropical and temperate areas (Aime, Henkel, & Ryvarden, 2003; ported by phylogenetic analysis inferred from the nrDNA Internal Valenzuela et al., 2012), Asia (Bian & Dai, 2015; Corner, 1991; Dai, Transcribed Spacer (ITS) region. 2010; Dai & Li, 2012; Dai, Cui, He, & Schigel, 2014; Dai & Niemela,€ Macroscopic and microscopic features of basidiocarps collected 2006), and Australia (Reid, 1963). Species of Coltriciella are from Haurbentes Experimental Forest in Bogor, West Java, were commonly found on wood (Aime et al., 2003; Corner, 1991; observed in fresh and dried conditions. All the materials were examined under a Nikon 80i microscope (Nikon Corporation, Tokyo, Japan). Line drawings were made with the aid of a Nikon Y- * Corresponding author. IDN drawing tube (Nikon Corporation). Slides were prepared from E-mail address: [email protected] (N. Sukarno). https://doi.org/10.1016/j.myc.2017.08.005 1340-3540/© 2017 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. 50 D. Susan et al. / Mycoscience 59 (2018) 49e53 dried specimens mounted in 5% (w/v) KOH solution, 1% (w/v) Huelsenbeck, 2003). The best-fit model for the dataset was phloxine solution, Melzer's reagent (0.5 g iodine, 1.5 g potassium selected by jModelTest 2.1.5 (Darriba, Taboada, Doallo, & Posada, iodide, 22 g chloral hydrate, 20 g water) and aniline blue solution 2012). Two independent runs with 1,000,000 generations were (0.1 mg aniline blue dissolved in 60 g pure lactic acid) (Ryvarden & performed, with sampling of every 100th generation. The first 25% Johansen, 1980). Abbreviations used in the morphological of trees were discarded while the last 75% of the trees were used to description were as follows: IKI (Melzer's reagent; with construct consensus trees. IKI¼inamyloid), CB (cotton blue; CBþ¼cyanophylous; CB¼acyanophylous), L (mean spore length), W (mean spore 1. Taxonomy width), Q (the L/W ratio) and n (number of spores measured). Color terminology followed that of Kornerup and Wanscher (1967). The Coltriciella minuscula Susan, Retnowati & Sukarno, sp. nov. examined specimens were afterwards deposited in Herbarium Figs. 1 and 2. Bogoriense (BO), Botany Division, Research Center for Biology, MycoBank no.: MB 817889. Indonesian Institute of Sciences. Basidiocarps annual, pendent, 1e4 mm diam, stipe short, fibrillose, An attempt was made to culture the fungi on Modified Merlin hirsute. Hyphal system monomitic. Basidiospores ellipsoid, golden Norkrans agar medium, however, fungal growth did not occur. brown, finely verruculose. Dried material of the fungus (ca. 10 mg) was ground to powder Type: INDONESIA, West Java, Bogor, Haurbentes Experimental with sterile sand. A Genomic DNA Mini Kit (Plant) (Geneaid Biotech Forest, on trunk base of Pinus merkusii, 4 Jan 2014, leg. D. Susan Ltd., New Taipei City, Taiwan) was used to extract DNA from the DWS1192 (holotype, BO22806). dried specimen following the manufacturers' instructions. ITS re- Gene sequences ex-holotype: KX086684 (ITS). gion sequences were obtained using primer sets ITS1F/ITS4B Etymology: minuscula, referring to the size of the basidiocarp. (Gardes & Bruns, 1993). Reactions were performed with the Basidiocarps pendent, light in weight. Stipe dorsally attached or following cycling parameters: initial denaturation at 94 C for 1 min, then 35 cycles at 94 C for 30 s, annealing at 51 C for 1 min and extension at 72 C for 1 min, with a final extension at 72 C for 10 min. The amplicons were sequenced by 1st BASE (Selangor, Malaysia) using primer sets ITS1F/ITS4B. The ITS sequences obtained in this study were deposited under GenBank accession Nos. KX086684 for Coltriciella minuscula and KX159769 for C. aff. subglobosa. Each sequence was compared to the reference sequences in GenBank, NCBI using BLAST search (http:// blast.ncbi.nlm.nih.gov/Blast.cgi). The fungal taxa used in the phylogenetic analysis are listed in Table 1. The sequences were then aligned to those sequences downloaded from GenBank using MEGA. Phylogenetic analyses were performed using maximum parsimony and Bayesian inference. Maximum Parsimony analysis was conducted with PAUP* v.4.0b10 (Swofford, 2003). All charac- ters were equally weighted and gaps were treated as missing data. Trees were inferred using the heuristic search option with TBR branch brach swapping. Clade robustness was assessed using bootstrap analysis with 1000 replicates (Felsenstein, 1985). & Bayesian analysis was performed with MrBayes 3.2.1 (Ronquist Fig. 1. Basidiocarps of Coltriciella minuscula (BO22806). Bar: 0.5 cm.

Table 1 e List of GenBank accession numbers of sequences used in the phylogenetic analysis.

Species name Specimen reference Origin GenBank accession no.

Coltriciella baoshanensis Dai 13075 China, Yunnan, Gaogling Mts. KC857266 C. baoshanensis Dai13072 China KU360700 C. dependens TU103078 Seychelles, Mahe, Casse Dent AM412254 C. dependens TAA195099 Seychelles, Mahe, L'Abondance AM412253 C. dependens TU103078 China, Fujian, Fuzhou AM412252 C. globosa L.S. Bian & Y.C. Dai Cui 7545 China, Guangdong, Ruyang KJ540930 C. navispora T.W. Henkel, Aime & Ryvarden TH9529 Guyana KT339262 C. navispora MCA3921 Guyana KC155386 C. oblectabilis (Lloyd) Kotl., Pouzar & Ryvarden TH9187 Guyana KC155387 C. pseudodependens Cui 8138 China, Yunnan, Baoshan KJ540931 C. pusilla (Imazeki & Kobayashi) Corner Dai15168 China KU360701 C. subglobosa Y.C. Dai Dai15158 China KU360702 Coltricia confluens P.-J. Keizer TAA181460 Estonia AM412241 C. confluens GO-2009-444 Mexico, Puebla, Chalchicomula De Sesma KC152085 C. confluens GO-2009-008 Mexico, Mexico State, Temascaltepec KC152083 C. confluens GO-2009-483 Mexico, Tlaxcla, Huamantla KC152084 C. perennis (L.) Murrill AFTOL-ID 447 clone 2 DQ234560 C. perennis AFTOL-ID 447 clone 1 DQ234559 C. perennis AFTOL-ID 447 clone 3 DQ234561 Inonotus zonatus Y.C. Dai & X.M. Tian Cui 6631 China JQ860305 Trametes elegans (Spreng.) Fr. USA AY684178 Phellinus fragrans M.J. Larsen & Lombard CBS 202.90 USA AY558619 Download English Version: https://daneshyari.com/en/article/8391912

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