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RNASEH1 Polyclonal antibody Catalog Number:15606-1-AP Featured Product 25 Publications www.ptglab.com

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 15606-1-AP BC002973 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 450 μg/ml by 246243 WB 1:500-1:2400 Nanodrop and 333 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:1000 method using BSA as the standard; H1 for WB IF 1:50-1:500 Source: Calculated MW: Rabbit 32 kDa Isotype: Observed MW: IgG 32 kDa Immunogen Catalog Number: AG7990

Applications Tested Applications: Positive Controls: IF, IP, WB,ELISA WB : HeLa cells, human brain tissue Cited Applications: IP : HeLa cells, IF, WB IF : HeLa cells, Species Specificity: human, mouse, rat Cited Species: human, mouse

Ribonucleases H are that cleave the RNA of RNA/DNA hybrids that form during replication and repair and Background Information which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Notable Publications Author Pubmed ID Journal Application Sarah J Hill 25184681 Dev IF Sarah J Hill 27849576 Proc Natl Acad Sci U S A IF Flavia Michelini 29180822 Nat Cell Biol IF

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

HeLa cells were subjected to SDS PAGE followed by IP Result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Immunofluorescent analysis of (-20 Ethanol) fixed western blot with 15606-1-AP (RNASEH1 antibody) Detection:15606-1-AP 1:500) with HeLa cells lysate HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature 1200ug. at dilution of 1:50 and Alexa Fluor 488-conjugated for 1.5 hours. AffiniPure Goat Anti-Rabbit IgG(H+L).