PPP1CB Polyclonal Antibody

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For Research Use Only PPP1CB Polyclonal antibody Catalog Number:55136-1-AP Featured Product 2 Publications www.ptgcn.com Catalog Number: GenBank Accession Number: Recommended Dilutions: Basic Information 55136-1-AP NM_002709 WB 1:1000-1:4000 Size: GeneID (NCBI): IF 1:50-1:500 1600 μg/ml 5500 Source: Full Name: Rabbit protein phosphatase 1, catalytic Isotype: subunit, beta isoform IgG Calculated MW: Purification Method: 37 kDa Antigen affinity purification Observed MW: Immunogen Catalog Number: 37-40 kDa, 33 kDa Applications Tested Applications: Positive Controls: IF, WB, ELISA WB : A549 cells; mouse brain tissue Cited Applications: IF : A549 cells; WB Species Specificity: human, mouse, rat Cited Species: human PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase Background Information (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB. Notable Publications Author Pubmed ID Journal Application Zhanying Hu 32702076 PLoS Pathog WB Yang Guo 34280391 Exp Eye Res WB Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 4006900926 E: [email protected] W: ptgcn.com Group brand and is not available to purchase from any other manufacturer. Selected Validation Data A549 cells were subjected to SDS PAGE followed by Immunofluorescent analysis of (10% western blot with 55136-1-AP (PPP1CB antibody) at Formaldehyde) fixed A549 cells using 55136-1-AP dilution of 1:2000 incubated at room temperature (PPP1CB antibody) at dilution of 1:50 and Alexa for 1.5 hours. Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)..

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Integrative Genomics Analysis of Eqtl and GWAS Summary Data Identifies
Bioscience Reports (2020) 40 BSR20193185 https://doi.org/10.1042/BSR20193185 Research Article Integrative genomics analysis of eQTL and GWAS summary data identifies PPP1CB as a novel bone mineral density risk genes Yu Zhai1,2,*,LuYu1,*, Yang Shao2 and Jianwei Wang2 Downloaded from http://portlandpress.com/bioscirep/article-pdf/40/4/BSR20193185/872280/bsr-2019-3185.pdf by guest on 01 October 2021 1Changzhou Hospital Affiliated to Nanjing University of Chinese Medicine, Changzhou 213003, Jiangsu, China; 2Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi 214071, Jiangsu, China Correspondence: Jianwei Wang ([email protected]) In recent years, multiple genome-wide association studies (GWAS) have identified numerous susceptibility variants and risk genes that demonstrate significant associations with bone mineral density (BMD). However, exploring how these genetic variants contribute risk to BMD remains a major challenge. We systematically integrated two independent expression quantitative trait loci (eQTL) data (N = 1890) and GWAS summary statistical data of BMD (N = 142,487) using Sherlock integrative analysis to reveal whether expression-associated vari- ants confer risk to BMD. By using Sherlock integrative analysis and MAGMA gene-based analysis, we found there existed 36 promising genes, for example, PPP1CB, XBP1, and FDFT1, whose expression alterations may contribute susceptibility to BMD. Through a protein–protein interaction (PPI) network analysis, we further prioritized the PPP1CB as a hub gene that has interactions with predicted genes and BMD-associated genes. Two eS- −17 −11 NPs of rs9309664 (PeQTL = 1.42 × 10 and PGWAS = 1.40 × 10 ) and rs7475 (PeQTL = −6 −7 2.10 × 10 and PGWAS = 1.70 × 10 )inPPP1CB were identified to be significantly as- sociated with BMD risk.
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Hepatitis C Virus As a Unique Human Model Disease to Define
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WO 2019/079361 Al 25 April 2019 (25.04.2019) W 1P O PCT
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Single Cell Analysis of the HIV-1 Latent Reservoir Lillian Brumer Cohn
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SHOC2–MRAS–PP1 complex positively regulates RAF activity and contributes to Noonan syndrome pathogenesis Lucy C. Younga,1, Nicole Hartiga,2, Isabel Boned del Ríoa, Sibel Saria, Benjamin Ringham-Terrya, Joshua R. Wainwrighta, Greg G. Jonesa, Frank McCormickb,3, and Pablo Rodriguez-Vicianaa,3 aUniversity College London Cancer Institute, University College London, London WC1E 6DD, United Kingdom; and bHelen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA 94158 Contributed by Frank McCormick, September 18, 2018 (sent for review November 22, 2017; reviewed by Deborah K. Morrison and Marc Therrien) Dephosphorylation of the inhibitory “S259” site on RAF kinases CRAF/RAF1 mutations are also frequently found in NS and (S259 on CRAF, S365 on BRAF) plays a key role in RAF activation. cluster around the S259 14-3-3 binding site, enhancing CRAF ac- The MRAS GTPase, a close relative of RAS oncoproteins, interacts tivity through disruption of 14-3-3 binding (8) and highlighting the with SHOC2 and protein phosphatase 1 (PP1) to form a heterotri- key role of this regulatory step in RAF–ERK pathway activation. meric holoenzyme that dephosphorylates this S259 RAF site. MRAS is a very close relative of the classical RAS oncoproteins MRAS and SHOC2 function as PP1 regulatory subunits providing (H-, N-, and KRAS, hereafter referred to collectively as “RAS”) the complex with striking specificity against RAF. MRAS also func- and shares most regulatory and effector interactions as well as tions as a targeting subunit as membrane localization is required transforming ability (9–11). However, MRAS also has specific for efficient RAF dephosphorylation and ERK pathway regulation functions of its own, and uniquely among RAS family GTPases, it in cells.
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Blueprint Genetics Noonan Syndrome Panel
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Datasheet (PDF)
PP1B, active, GST-tagged, human recombinant, expressed in Sf9 cells Catalog Number SRP5337 Storage Temperature –70 °C Synonyms: PP-1B, PPP1CB, PPP1CD, MGC3672 Figure 1. SDS-PAGE Gel of Typical Lot: Product Description ³70% (SDS-PAGE, densitometry) PP1B or protein phosphatase 1, catalytic subunit, beta isozyme is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1B is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription.1 PP1B is also known as PP1-delta. PP1B has been reported to functions as a suppressor of learning and memory.2 Recombinant full-length human PP1B was expressed by baculovirus in Sf9 insect cells using an N-terminal Figure 2. GST-tag. The gene accession number is NM_002709. Specific Activity of Typical Lot: It is supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 218–326 nmole/min/mg 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol. Molecular mass: ~60 kDa Phosphatase activity was determined with a spectrophotometric assay procedure. Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material References Safety Data Sheet for information regarding hazards 1. Barker, H.M. et al., Three genes for protein and safe handling practices. phosphatase 1 map to different human chromosomes: sequence, expression and gene Storage/Stability localisation of protein serine/threonine phosphatase The product ships on dry ice and storage at –70 °C is 1 beta (PPP1CB).
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Human Genetics (2019) 138:21–35 https://doi.org/10.1007/s00439-018-1951-7 ORIGINAL INVESTIGATION Delineation of LZTR1 mutation-positive patients with Noonan syndrome and identification of LZTR1 binding to RAF1–PPP1CB complexes Ikumi Umeki1 · Tetsuya Niihori1 · Taiki Abe1 · Shin‑ichiro Kanno2 · Nobuhiko Okamoto3 · Seiji Mizuno4 · Kenji Kurosawa5 · Keisuke Nagasaki6 · Makoto Yoshida7 · Hirofumi Ohashi8 · Shin‑ichi Inoue1 · Yoichi Matsubara9 · Ikuma Fujiwara10 · Shigeo Kure11 · Yoko Aoki1 Received: 20 July 2018 / Accepted: 21 October 2018 / Published online: 27 October 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract RASopathies are a group of developmental disorders caused by mutations in genes that regulate the RAS/MAPK pathway and include Noonan syndrome (NS), Costello syndrome, cardiofaciocutaneous syndrome and other related disorders. Whole exome sequencing studies recently identified LZTR1, PPP1CB and MRAS as new causative genes in RASopathies. However, information on the phenotypes of LZTR1 mutation-positive patients and functional properties of the mutations are limited. To identify variants of LZTR1, PPP1CB, and MRAS, we performed a targeted next-generation sequencing and reexamined previously analyzed exome data in 166 patients with suspected RASopathies. We identified eight LZTR1 variants, including a de novo variant, in seven probands who were suspicious for NS and one known de novo PPP1CB variant in a patient with NS. One of the seven probands had two compound heterozygous LZTR1 variants, suggesting autosomal recessive inheritance. All probands with LZTR1 variants had cardiac defects, including hypertrophic cardiomyopathy and atrial septal defect. Five of the seven probands had short stature or intellectual disabilities. Immunoprecipitation of endogenous LZTR1 followed by western blotting showed that LZTR1 bound to the RAF1–PPP1CB complex.
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