DOCSLIB.ORG

Endogenous Cytokinins in Medicinal Basidiomycetes Mycelial Biomass

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Endogenous Cytokinins in Medicinal Basidiomycetes Mycelial Biomass Experimental articles UDK 606:615.322:631:871 doi: 10.15407/biotech9.01.055 ENDOGENOUS CYTOKININS IN MEDICINAL BASIDIOMYCETES MYCELIAL BIOMASS N. P. Vedenicheva G. A. Al-Maali N. Yu. Mytropolska Kholodny Institute of Botany of the National Academy O. B. Mykhaylova of Sciences of Ukraine, Kyiv N. A. Bisko I. V. Kosakivska E-mail: [email protected] Received 10.02.2016 The aim of the research was to study the cytokinins production by medicinal basidial mushrooms. Cytokinins were for the first time identified and quantified in mycelial biomass of six species (Ganoderma lucidum, Trametes versicolor, Fomitopsis officinalis, Pleurotus nebrodensis, Grifola frondosa, Sparassis crispa) using HPLC. Trans- and cis-zeatin, zeatin riboside, zeatin-O-glucoside, isopentenyladenosine, isopentenyladenine were found but only one species (G. lucidum, strain 1900) contained all these substances. The greatest total cytokinin quantity was detected in F. officinalis, strain 5004. S. crispa, strain 314, and F. officinalis, strain 5004, mycelial biomass was revealed to have the highest level of cytokinin riboside forms (zeatin riboside and isopentenyladenosine). The possible connection between medicinal properties of investigated basidiomycetes and of cytokinins is discussed. S. crispa, strain 314, and F. officinalis, strain 5004, are regarded as promising species for developing biotechnological techniques to produce biologically active drugs from their mycelial biomass. As one of the potential technological approaches there is proposed fungal material drying. Key words: medicinal mushrooms, mycelial biomass, cytokinins. Mushrooms form a special kingdom meristems initiation and activity, delay of living creature — Fungi. They possess leaves senescence, inhibit roots growth and both plant and animal features in their cell branching, control sink/source relationships, structure and metabolism. The most important seeds germination, nutrient uptake and regulation system of plants and animals is response to biotic and abiotic stresses [4, 5]. a hormonal one, all vital functions of these Naturally occurring cytokinins are adenine organisms are under hormonal control. derivatives which have the similar structure Hormones trigger all life programs without but not equivalent functions. Hormone exceptions and control their running during molecules with the side chain structure ontogenesis. The action of such system in fungi variations are likely to mediate different remains unknown. More than 35 fungi species biological signals. The participation of trans- are able to synthesize the phytohormones, but zeatin and isopentenyladenine in long-distance a functional significance of these substances signal transmission in acropetal and basipetal has not been investigated [1]. The main directions along plant stem correspondently is attention was concentrated on microscopic assumed [6]. phytopathogenic fungi causing an abnormal Phytopathogenic fungi are shown to growth of host plants tissues and tumours produce high concentrations of cytokinins in formation. One of the specific symptoms of culture [7, 8]. The incorporation of 14C-adenine fungal infection is a considerable cytokinins into cytokinins in Fusarium moniliforme levels increase in these tissues (several times confirmed the ability of this fungus to higher than those in healthy ones) [2, 3]. synthesize hormone de novo [9]. Biotrophic Cytokinins are polyfunctional fungus Claviceps purpurea produced phytohormones that are involved in plants substantial quantities of cytokinins in culture growth and development regulation. They and expressed the cytokinins biosynthesis stimulate cells division and shoot apical enzymes genes [2]. The general strategy of 55 BIOTECHNOLOGIA ACTA, V. 9, No 1, 2016 parasitic fungi is to use cytokinins for host Materials and Methods plant growth manipulations. They not only Biological material can apply hormones synthesized by themselves The following basidial macromycetes were but change cytokinins biosynthesis genes screened for cytokinins content: Pleurotus expression in occupied plant [10, 11]. nebrodensis (Inzenga) Quel. , Grifola frondosa Until now there are few data on the (Dicks: Fr.) S.F.Gray, Fomitopsis officinalis cytokinins occurrence and functioning in (Vill.) Bondartsev et Singer, Sparassis crispa basidial mushrooms. Cytokinins activity (Fr.) Fr., Ganoderma lucidum (Curtis) P. Karst was for the first time detected in their and Trametes versicolor (L.) Lloyd. All of fruit bodies by bioassays in the 70s of the them are the valuable fungi species with long XX century. Cytokinins activity corresponding history of applying in oriental medicine and to zeatin and zeatin riboside was detected in ethnoscience for many diseases treatment and Agaricus bisporus (J. E. Lange) Imbach and prevention. We investigated the pure cultures Pleurotus sajor-caju (Fr.) Singer [12], in six of F. officinales 5004, S. crispa 314, G. lucidum species of genus Rhizopogon and twenty two 1900, T. versicolor 353, G. frondosa 976 and species of genus Hebeloma [13]. The content P. nebrodensis 2035 from the National of zeatin riboside in Pleurotus ostreatus Ukrainian Culture Collection of Mushrooms of (Jacq.) P. Kumm. basidiomes [14], zeatin — Kholodny Institute of Botany of the National in Lentinus tigrinus (Bull.) Fr. and Laetiporus Academy of Sciences of Ukraine. sulphurous (Bull.) Murrill [15], trans- zeatin — in eighteen macromycetes species, Mushrooms cultivation particularly in L. tigrinus, Boletus impolitus To obtain the mycelial biomass of above Fr., Ptychoverpa bohemica (Krombh.) Boud., mentioned strains we cultivated them in 250 ml Volvariella speciosa (Fr.) Singer, Amanita Erlenmeyer flasks with 50 ml liquid medium at gemmata (Fr.) Bertill. etc. was determined thermostat conditions (26 ± 1 C) in darkness by high performance liquid chromatography (Fig. 1). Inoculation with physiologically (HPLC) [16]. Recently, combination of HPLC active mycelium in proportion 10% to total with electrospray ionization tandem mass volume was carried out in accordance with spectrometry allowed to determine seven cytokinins in twenty species of forest fungi [3]. method developed for basidiomycetes [21]. Last years, a higher interest to growth Microbiological control of nutrient medium regulating substances in edible mushrooms and seeding material purity was fulfilled fruit bodies is connected with necessity to before inoculation. For cultivation of F. offi- produce ecologically pure protein products cinales 5004 and S. crispa 314 the following and to increase cultivated mushrooms yield. liquid nutrient medium was used (g/l): Additionally, almost all basidiomycetes possess glucose — 30,0; NH4 NO3 — 3,5; KCl — 0,5; the medicinal properties and that is why K2HPO4 — 1,0; MgSO4·7H2O — 0,5; beer wort medicinal mushrooms science devoted to study (15 in accordance with Baling method) — of these fungi characteristics is developing 115 ml; рН — 5,0. For cultivation of G. lucidum successfully in 21st century [17]. It is not yet 1900, T. versicolor 353, G. frondosa 976 and completely known what specific components P. nebrodensis 2035 such liquid nutrient medium of biochemical mushrooms composition are was used (g/l): glucose — 25,0; peptone — 3,0; effective therapeutic agents. At the same yeast extract — 3,0; KH2PO4 — 1,0; K2HPO4 — time, therapeutic properties of cytokinins, in 1,0l; MgSO4·7H2O — 0,25; рН — 6,5. Mediums particular, anticancer and immunomodulatory acidity was maintained at necessary pH levels action, have been proved now [18–20]. The by 1 N КОН and 1 N НСl solutions addition. question about possible interco nnection between medicinal mushrooms effects and Cytokinins analysis cytokinins synthesized in their cells arises. The cytokinins extraction and purification We have previously established that cytokinins from mushrooms mycelial biomass and final content in Lentinus edodes (Berk.) Singer determination in samples were based on the mycelium is by an order in magnitude higher methods described for plants [22] with some than that of fruit bodies (data not published). modifications. Choice and combination of Taking in consideration all above mentioned procedures and their adaptation to work with we aimed to study the cytokinin production mushrooms mycelial biomass were realized for by mycelial biomass of basidial mushrooms the first time. The sample (10 g of mycelial and to determine the species with the higher biomass) was homogenized in 80% ethanol productivity. solution. Homogenous material was mixed 56 Experimental articles Bio-Rad (USA) containing ionexchange resin A Dowex 50Wx8 (Serva, Germany) in H+ form. The column was rinsed with water, the sample was eluated by 0,1 N ammonia and eluate was evaporated under vacuum. Then, sample was purified by thin layer chromatography (TLC). The dry residue was dissolved in 1 ml of 96% ethanol and applied on TLC plates Silicagel 60 F254 (Merck, Germany) 2020 cm. The plates were run in solvent systems isopropanol:ammonia:water (10:1:1 by volume) and dried. Zones correspondent to the cytokinins standards were detected under UV 254 in chemoscope Desaga (Germany) and then eluated with 96% ethanol. The final cytokinins identification and determination were performed by HPLC (Agilent 1200 LC, USA) using Eclipse XDB-C 18 column (2,1150 mm), particles size 5 μм. Elution was carried out with solvents system methanol:water (37:63 B by volume). Data were analyzed and processed by software Chem Station, version В.03.01 on line. Standard solutions of trans- and cis- zeatin,
Load more

Recommended publications
  • Appendix K. Survey and Manage Species Persistence Evaluation
    Appendix K. Survey and Manage Species Persistence Evaluation Establishment of the 95-foot wide construction corridor and TEWAs would likely remove individuals of H. caeruleus and modify microclimate conditions around individuals that are not removed. The removal of forests and host trees and disturbance to soil could negatively affect H. caeruleus in adjacent areas by removing its habitat, disturbing the roots of host trees, and affecting its mycorrhizal association with the trees, potentially affecting site persistence. Restored portions of the corridor and TEWAs would be dominated by early seral vegetation for approximately 30 years, which would result in long-term changes to habitat conditions. A 30-foot wide portion of the corridor would be maintained in low-growing vegetation for pipeline maintenance and would not provide habitat for the species during the life of the project. Hygrophorus caeruleus is not likely to persist at one of the sites in the project area because of the extent of impacts and the proximity of the recorded observation to the corridor. Hygrophorus caeruleus is likely to persist at the remaining three sites in the project area (MP 168.8 and MP 172.4 (north), and MP 172.5-172.7) because the majority of observations within the sites are more than 90 feet from the corridor, where direct effects are not anticipated and indirect effects are unlikely. The site at MP 168.8 is in a forested area on an east-facing slope, and a paved road occurs through the southeast part of the site. Four out of five observations are more than 90 feet southwest of the corridor and are not likely to be directly or indirectly affected by the PCGP Project based on the distance from the corridor, extent of forests surrounding the observations, and proximity to an existing open corridor (the road), indicating the species is likely resilient to edge- related effects at the site.
    [Show full text]
  • SOMA News March 2011
    VOLUME 23 ISSUE 7 March 2011 SOMA IS AN EDUCATIONAL ORGANIZATION DEDICATED TO MYCOLOGY. WE ENCOURAGE ENVIRONMENTAL AWARENESS BY SHARING OUR ENTHUSIASM THROUGH PUBLIC PARTICIPATION AND GUIDED FORAYS. WINTER/SPRING 2011 SPEAKER OF THE MONTH SEASON CALENDAR March Connie and Patrick March 17th » Meeting—7pm —“A Show and Tell”— Sonoma County Farm Bureau Speaker: Connie Green & Patrick March 17th—7pm Hamilton Foray March. 19th » Salt Point April April 21st » Meeting—7pm Sonoma County Farm Bureau Speaker: Langdon Cook Foray April 23rd » Salt Point May May 19th » Meeting—7pm Sonoma County Farm Bureau Speaker: Bob Cummings Foray May: Possible Morel Camping! eparated at birth but from the same litter Connie Green and Patrick Hamilton have S traveled (endured?) mushroom journeys together for almost two decades. They’ve been to the humid and hot jaguar jungles of Chiapas chasing tropical mushrooms and to EMERGENCY the cloud forests of the Sierra Madre for boletes and Indigo milky caps. In the cold and wet wilds of Alaska they hiked a spruce and hemlock forest trail to watch grizzly bears MUSHROOM tearing salmon bellies just a few yards away. POISONING IDENTIFICATION In the remote Queen Charlotte Islands their bush plane flew over “fields of golden chanterelles,” landed on the ocean, and then off into a zany Zodiac for a ride over a cold After seeking medical attention, contact and roiling sea alongside some low flying puffins to the World Heritage Site of Ninstints. Darvin DeShazer for identification at The two of them have gazed at glaciers and berry picked on muskeg bogs. More than a (707) 829-0596.
    [Show full text]
  • Effects of Sparassis Crispa in Medical Therapeutics: a Systematic Review and Meta-Analysis of Randomized Controlled Trials
    International Journal of Molecular Sciences Article Effects of Sparassis crispa in Medical Therapeutics: A Systematic Review and Meta-Analysis of Randomized Controlled Trials Le Thi Nhu Ngoc 1, You-Kwan Oh 2, Young-Jong Lee 3,* and Young-Chul Lee 1,* ID 1 Department of BioNano Technology, Gachon University, 1342 Seongnam-Daero, Sujeong-Gu, Seongnam-Si, Gyeonggi-do 13120, Korea; [email protected] 2 School of Chemical and Biomolecular Engineering, Pusan National University, 2 Busandaehak-ro, Geumjeong-Gu, Busan 46241, Korea; [email protected] 3 Department of Herbology, College of Korean Medicine, Gachon University, 1342 Seongnam-Daero, Sujeong-Gu, Seongnam-Si, Gyeonggi-do 13120, Korea * Correspondence: [email protected] (Y.-J.L.); [email protected] (Y.-C.L.); Tel.: +82-31-750-5415 (Y.-J.L.); +82-31-750-8751 (Y.-C.L.); Fax: +82-31-750-5416 (Y.-J.L.); +82-31-750-4748 (Y.-C.L.) Received: 31 March 2018; Accepted: 9 May 2018; Published: 16 May 2018 Abstract: In this study, we investigated the therapeutic potential and medical applications of Sparassis crispa (S. crispa) by conducting a systematic review of the existing literature and performing a meta-analysis. The original efficacy treatment of the mushroom extract is considered primarily and searched in electronic databases. A total of 623 articles were assessed, 33 randomized controlled experiments were included after the manual screening, and some papers, review articles, or editorials that did not contain data were excluded. A comparative standard means difference (SMD) and a funnel plot between control and S. crispa groups were used as parameters to demonstrate the beneficial effects of S.
    [Show full text]
  • Review Article Natural Products and Biological Activity of the Pharmacologically Active Cauliflower Mushroom Sparassis Crispa
    Hindawi Publishing Corporation BioMed Research International Volume 2013, Article ID 982317, 9 pages http://dx.doi.org/10.1155/2013/982317 Review Article Natural Products and Biological Activity of the Pharmacologically Active Cauliflower Mushroom Sparassis crispa Takashi Kimura Research&DevelopmentCenter,UnitikaLtd.,23Uji-Kozakura,Uji,Kyoto611-0021,Japan Correspondence should be addressed to Takashi Kimura; [email protected] Received 4 October 2012; Accepted 25 February 2013 Academic Editor: Fabio Ferreira Perazzo Copyright © 2013 Takashi Kimura. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Sparassis crispa, also known as cauliflower mushroom, is an edible mushroom with medicinal properties. Its cultivation became popular in Japan about 10 years ago, a phenomenon that has been attributed not only to the quality of its taste, but also to its potential for therapeutic applications. Herein, I present a comprehensive summary of the pharmacological activities and mechanisms of action of its bioactive components, such as beta-glucan, and other physiologically active substances. In particular, the immunomodulatory mechanisms of the beta-glucan components are presented herein in detail. 1. Introduction 2. Chemical Constituents and Bioactive Components of S. crispa Medicinal mushrooms have an established history of use in traditional Asian therapies. Over the past 2 to 3 decades, Scientific investigation has led to the isolation of many com- scientific and medical research in Japan, China, and Korea, pounds from S. crispa that have been shown to have health- and more recently in the United States, has increasingly promoting activities.
    [Show full text]
  • Conservation Status Assessment
    Element Ranking Form Oregon Biodiversity Information Center Conservation Status Assessment Scientific Name: Sparassis radicata Classification: Fungus Assessment area: Global Heritage Rank: G4 Rank Date: 6/15/2018 Rank Reasons: Name now Sparassis radicata (previously Sparassis crispa) based on presentation at the Oregon Mycological Society, 2/23/2015; Wang, Z., et al., 2004 supports that Sparassis radicata is our western cauliflower mushroom and Sparassis crispa is the eastern counterpart; Index Fungorum and Mycobank still have Sparassis crispa (1821) as the current name and Sparassis radicata (1917) as a synonym. S. Loring is unsure how to handle this species, globally or by each state. This is not a rare species on the west coast, but goes extremely under-reported to agency databases and herbariums. Loring frequently sees it throughout forested areas of the PNW. It turns up multiple times at nearly all forays in the PNW. It is a prized edible and commonly documented via online mushrooms forums. Range Extent: H = >2,500,000 sq km (> 1,000,000 sq mi) Comments: Previously considered a synonym of Sparassis crispa, Sparassis radicata has been determined in recent genetic studies to be a distinct species separate from Sparassis crispa specimens in Europe and Asia (Wang et al., 2004). Sparassis radicata present in Northern California to southern British Columbia; Idaho; disjunct sites in eastern North America: Tennessee (Wang et al., 2004), and Georgia (cited in Light and Woehrel, 2009); additional sites certaintly present. Given these S. radicata sites, the range is well over 2.5 million sq km. Population Size: Not assessed Comments: None Number of Occurrences: D = 81 - 300 Comments: Given the relatively recent taxonomic change with this species, exact counts are unknown, but it is an often-encountered species in the Pacific Northwest and likely falls within this range.
    [Show full text]
  • A Revised Family-Level Classification of the Polyporales (Basidiomycota)
    fungal biology 121 (2017) 798e824 journal homepage: www.elsevier.com/locate/funbio A revised family-level classification of the Polyporales (Basidiomycota) Alfredo JUSTOa,*, Otto MIETTINENb, Dimitrios FLOUDASc, € Beatriz ORTIZ-SANTANAd, Elisabet SJOKVISTe, Daniel LINDNERd, d €b f Karen NAKASONE , Tuomo NIEMELA , Karl-Henrik LARSSON , Leif RYVARDENg, David S. HIBBETTa aDepartment of Biology, Clark University, 950 Main St, Worcester, 01610, MA, USA bBotanical Museum, University of Helsinki, PO Box 7, 00014, Helsinki, Finland cDepartment of Biology, Microbial Ecology Group, Lund University, Ecology Building, SE-223 62, Lund, Sweden dCenter for Forest Mycology Research, US Forest Service, Northern Research Station, One Gifford Pinchot Drive, Madison, 53726, WI, USA eScotland’s Rural College, Edinburgh Campus, King’s Buildings, West Mains Road, Edinburgh, EH9 3JG, UK fNatural History Museum, University of Oslo, PO Box 1172, Blindern, NO 0318, Oslo, Norway gInstitute of Biological Sciences, University of Oslo, PO Box 1066, Blindern, N-0316, Oslo, Norway article info abstract Article history: Polyporales is strongly supported as a clade of Agaricomycetes, but the lack of a consensus Received 21 April 2017 higher-level classification within the group is a barrier to further taxonomic revision. We Accepted 30 May 2017 amplified nrLSU, nrITS, and rpb1 genes across the Polyporales, with a special focus on the Available online 16 June 2017 latter. We combined the new sequences with molecular data generated during the Poly- Corresponding Editor: PEET project and performed Maximum Likelihood and Bayesian phylogenetic analyses. Ursula Peintner Analyses of our final 3-gene dataset (292 Polyporales taxa) provide a phylogenetic overview of the order that we translate here into a formal family-level classification.
    [Show full text]
  • Combination of Beta Glucan, Honey and Chlorhexidine in the Wound Management in a Cat a Case Report
    DOI: 10.2478/fv-2019-0040 FOLIA VETERINARIA, 63, 4: 70—77, 2019 COMBINATION OF BETA GLUCAN, HONEY AND CHLORHEXIDINE IN THE WOUND MANAGEMENT IN A CAT A CASE REPORT Micháľová, A.1, Micháľ, M.2, Fialkovičová, M.1 1University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice 2Animed s.r.o., Borovianska 76, 960 01 Zvolen Slovakia [email protected] ABSTRACT ability to moisturize defective tissues, and to make a pro- tective film that hinder the intersection of impurities Wound management is one of the oldest and one and decrease secondary contamination, are the benefits of the most frequent therapeutic activities in medi- of a gel formulation, that is the most appropriate exter- cine. Over the centuries there has been described and nal form of application in veterinary practice that can tested many therapeutic substances for the treatment improve and accelerate a successful healing process of of wounds with various effects. Due to the discovery of wounds in animals. antibiotics, a wound management regime used to be lim- ited only to a local application. Over years, it has been Key words: beta glucan; chlorhexidine digluconate; shown, that comprehensive therapy which uses only an- honey; wounds tibacterial preparations, also may contain some negative points (resistance of aggressive pathogens, toxicity, aller- gic reactions, etc.). According to studies, the best solu- INTRODUCTION tion to this problem is a local application, using prepara- tions that ensure the sterility of the affected parts of the Beta glucan skin, and the utilization of agents that are able to accel- Beta glucans belongs to prominent immunomodulators erate the granulation and lead to the healing process of and activators of white blood cells, especially macrophages the wound.
    [Show full text]
  • Chromosome-Scale Assembly of the Sparassis Latifolia Genome Obtained Using Long-Read and Hi-C Sequencing
    bioRxiv preprint doi: https://doi.org/10.1101/2021.01.08.426014; this version posted January 9, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Chromosome-scale assembly of the Sparassis latifolia genome obtained using 2 long-read and Hi-C sequencing 3 Chi Yang1, Lu Ma1, Donglai Xiao1, Xiaoyu Liu1, Xiaoling Jiang1, Zhenghe Ying1, 4 Yanquan Lin*1 5 1Institute of Edible Mushroom, Fujian Academy of Agricultural Sciences, Fuzhou 6 350012, China; National and Local Joint Engineering Research Center for Breeding & 7 Cultivation of Featured Edible Mushroom, Fujian Academy of Agricultural Sciences, 8 Fuzhou 350012, China 9 * Correspondence: 10 Corresponding Author 11 [email protected] 12 Keywords: Sparassis latifolia, genome, Hi-C sequencing, Oxford Nanopore 13 sequencing. 14 Abstract 15 Sparassis latifolia is a valuable edible mushroom cultivated in China. In 2018, our 16 research group reported an incomplete and low quality genome of S. latifolia was 17 obtained by Illumina HiSeq 2500 sequencing. These limitations in the available 18 genome have constrained genetic and genomic studies in this mushroom resource. 19 Herein, an updated draft genome sequence of S. latifolia was generated by Oxford 20 Nanopore sequencing and the Hi-C technique. A total of 8.24 Gb of Oxford Nanopore 21 long reads representing ~198.08X coverage of the S. latifolia genome were generated. 22 Subsequently, a high-quality genome of 41.41 Mb, with scaffold and contig N50 sizes 23 of 3.31 Mb and 1.51 Mb, respectively, was assembled.
    [Show full text]
  • December 2017
    MushRumors The Newsletter of the Northwest Mushroomers Association Volume 28, Issue 4 December 1, 2017 By Eric Worden This year’s Wild Mushroom Show was a great success in inspiring new community members to join our group! Thirty one new member/families signed up at the show. In addition, 16 existing members found it convenient to renew their memberships while at the show. Our current membership total is 226! However, due to the annual nature of the show, the seasonality of mushroom activities, and our calendar-year membership period (they expire on December 31), most (148) of our current members will have their memberships expire at the end of the year unless they renew. A graph of members by last pay date shows that this same pattern occurred last year. This graph evenly distributes all members across the chart horizontally, but positions them vertically by their last renewal date. We can see that a lot of people sign up or renew shortly before or during the show, then there is a lull during the winter, before club activities resume in the spring. Note that when you join or renew during September or later, your membership lasts through the following year. Our club relies on membership fees, in addition to Wild Mushroom Show revenue to pay for our club activities and expenses. To support the club, and to remain on the mailing list, please remember to renew! Annual memberships are $15 for families or $10 for students. If you forget, you will get a reminder email in the spring. You can renew online with a credit card or PayPal at http://www.northwestmushroomers.org/ join-or-renew-membership.
    [Show full text]
  • Sparassis Crispa (Wulf.) Fr., the Cauliflower Mushroom - a New Record from Lahore, Pakistan
    Mycopath (2007) 5(2): 119-120 Sparassis crispa (Wulf.) Fr., the cauliflower Mushroom - a new record from Lahore, Pakistan. Ghazala Nasim, Rukhsana Bajwa and Mohammad Ali Department of Mycology & Plant Pathology, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan. E-mail:[email protected] Abstract Sparassis crispa (Wulf.) Fr., the cauliflower mushroom, with densely branched fruiting body is a unique member among aphyllophorales. The present survey study reports it for the first time from Lahore, Pakistan. It was found growing in association with Melia azadaracta L. trees in the University of the Punjab, New Campus, Lahore. Keywords: Cauliflower mushroom, Sparassis crispa, Lahore, Pakistan. Introduction monomitic; arising from a large root-like sterile The present communication reports the Sparassis base, the upper portion appearing chambered crispa (Wulf.) Fr. (Cauliflower mushroom) from when sectioned, solid below; flesh white. Lahore, Pakistan collected on 23rd July 2006 Hymenium on the flattened surfaces of the from the base of a tree of a Melia azadarachta. fruiting body. Odor fragrant, somewhat spicy. This is being reported for the first time from Spores 5-7 × 3-5 µm elliptical, smooth, Lahore, Pakistan (Ahmad, 1956; Ahmad et al., colourless and non-amyloid. Spore deposit 1997). Previously it was reported from Swat, white. Usually solitary at the base of conifers, Northern Pakistan by Ahmad (1956; 1972) and especially Bishop and Monterey pine; fruiting Hattori and Murakami (1993). from late fall to mid-winter (Arora, 1991). It Sparassis crispa (Wulf.) Fr. (Syns. Sparassis usually grows from dead roots in the soil. It is radicata, Clavaria crispa), a member of the fleshy and aside from washing out the insects family Sparassidaceae, order Aphyllophorales is hiding under the numerous caps it is easy to commonly known as cauliflower mushroom.
    [Show full text]
  • Etiology Decay of Douglas-Fir by Sparassis Radicata in Arizona K. J
    Etiology Decay of Douglas-Fir by Sparassis radicata in Arizona K. J. Martin and R. L. Gilbertson Research Assistant and Professor, respectively, Department of Plant Pathology, University of Arizona, Tucson, AZ 85721. Present address of senior author: Arizona Commission of Agriculture and Horticulture, Phoenix, AZ 85007. Portion of a Ph.D. dissertation submitted by the senior author to the University of Arizona. University of Arizona Agricultural Experiment Journal Article No. 2717. Research supported by the College of Agriculture, University of Arizona, by an NDEA Title IV Fellowship awarded the senior author, and McIntire-Stennis Project No. 2016-4166-23. Accepted for publication 30 June 1977. ABSTRACT MARTIN, K. J., and R. L. GILBERTSON. 1978. Decay of Douglas-fir by Sparassis radicata in Arizona. Phytopathology 68: 149-154. Sparassis radicatais one of the major causes of a brown and homokaryotic isolates of S. radicata cause a brown root and butt rot of Douglas-fir in the Santa Catalina cubical decay in Douglas-fir and ponderosa pine wood Mountains of Arizona. Of 50 recently fallen Douglas-firs comparable to that found in naturally decayed roots and with a brown root and butt rot, decay was attributed to S. butts. Results of genetic analysis of mating-type factors radicatain 30% of the trees, to Phaeolus schweinitzii in 62% suggest that S. radicataspreads from tree to tree through root of the trees, and to both fungus species together in 8% of the grafts or root contacts. trees. Agar-block decay tests showed that both dikaryotic Additional key words: root-rotting fungi. Sparassis radicata Weir was described (17) from To determine relative decay capabilities of northwestern U.S.
    [Show full text]
  • (Basidiomycota): Evidence from Morphological and Molecular Data
    Mycol Progress (2013) 12:445–454 DOI 10.1007/s11557-012-0853-7 ORIGINAL ARTICLE New species and distinctive geographical divergences of the genus Sparassis (Basidiomycota): evidence from morphological and molecular data Qi Zhao & Bang Feng & Zhu L. Yang & Yu-Cheng Dai & Zheng Wang & B. Tolgor Received: 10 July 2012 /Revised: 8 August 2012 /Accepted: 10 August 2012 /Published online: 26 August 2012 # German Mycological Society and Springer 2012 Abstract Species of the genus Sparassis in East Asia were Keywords Cauliflower mushrooms . Morphology . investigated using morphology and DNA sequences data. Polyporales . Systematics . Taxonomy Phylogenetic analyses inferred from sequences of the inter- nal transcribed spacer (ITS), the nuclear gene coding for the ribosomal large subunit (nLSU) and partial gene coding Introduction RNA polymerase subunit II (rpb2) strongly supported line- ages corresponding to morphological features. Three taxa, S. The Cauliflower Mushrooms (Sparassis Fr.) include species subalpina, S. cystidiosa f. flabelliformis and S. latifolia were that produce flabellae with an amphigenous hymenium and a recognized from East Asia, and the former two taxa are new central mass giving rise to the flabellae (Desjardin et al. 2004; to science. The occurrence of S. latifolia in Japan and in the Wang et al. 2004). Members of Sparassis are distributed Russian Far East was confirmed. Geographical divergences mainly in temperate forests, and are well known in some of Sparassis in the Holarctic were observed. Most species regions of the world (Blanco-Dios et al. 2006; Burdsall and have relatively narrow distribution ranges, and taxa with Miller 1988; Dai et al. 2006; Desjardin et al. 2004;Kreisel intercontinental distributions were not detected.
    [Show full text]