The Use of Antimicrobial Agents for Treating Soft Tissues for Final Impression Procedures
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Y T E I C O S L ISSN 1107 - 1141 BALKAN JOURNAL OF STOMATOLOGY A IC G LO TO STOMA The Use of Antimicrobial Agents for Treating Soft Tissues for Final Impression Procedures SUMMARY Harry P. Papanagiotou, Dafni Pakou, Phophi Background. The response of periodontal tissues to prosthetic Kamposiora, George Papavasiliou, Myrto Mpriakou, Spiros Silvestros restorations, either temporary or permanent, has been an issue of major concern. Temporary restorations induce o slight to moderate inflammatory University of Athens, Dental School response of the adjacent soft tissues that could affect the quality of final Department of Prosthodontics, Athens, Greece impressions for fixed prostheses. The aim of this double-blind, randomized, 3 group parallel-designed clinical trial was to evaluate and compare the efficacy of 2 mouthrinses and a placebo on inhibiting inflammation around teeth bearing provisional restorations. A secondary objective was to evaluate if C31G could cause staining of the restorations and/or changes in taste perception. Material and Methods. The 2 mouthrinses used, were chlorhexidine digluconate and 0.3% C31G in an aqueous base. 28 patients underwent periodontal examination at baseline, which included assessment of the plaque index, bleeding on probing and discoloration index. 110 teeth prepared to receive fixed prosthetic restorations and provisional restorations were evaluated prior to final impression procedures. All patients were randomly assigned to 3 groups: the first group received 0.3% C31G in an aqueous base, the second group received chlorhexidine digluconate, and the third group received placebo. Statistical Analysis was performed by using both Multivariate Analysis of Variance (MANOVA) and Univariate Analysis of Variance (ANOVA) with repeated measures. The 4 dependent variables were plaque index, gingival index, surface coloration index and coloration severity index. The experimental factors were the administrated agent (between-subjects factor) and the intervals of consecutive measurements (within-subjects factor). Interaction between the experimental factors (therapeutic agent - repeated measures) was also examined. The level of statistical significance was set to 5%. Results. Both agents improved plaque index and bleeding on probing. The placebo had no efficacy in plaque control; 0.3% C31G in an aqueous base appeared to have positive control in plaque accumulation, superior than chlorhexidine digluconate. It was free of side effects, did not cause any staining of the teeth or induced an intrinsic bad taste. Conclusion. Within the limitations of this study, the results suggested that both mouthrinses were able to improve the condition of the periodontal tissues adjacent to teeth that bear provisional restorations, in order to prepare them for the final impression. C31G in an aqueous base had no side effects like staining of teeth. ORIGINAL PAPER (OP) Keywords: Final Impressions; Antimicrobial Agent; Staining Balk J Stom, 2011; 15:133-141 134 Harry P. Papanagiotou et al. Balk J Stom, Vol 15, 2011 Introduction influence on microflora constitution10; (2) Triclozan, which consists of a combination of diphenol and a germicidal According to the glossary of Prosthodontic Terms1, agent with low toxicity and wide antibacterial action - impression is a negative likeness or copy in reverse of the studies showed moderate effectiveness in the reduction of 9 surface of an object, an imprint of the teeth and adjacent plaque accumulation, mostly because of its rapid release ; structures for use in dentistry. The purpose is to fabricate (3) Chlorhexidine, which is considered to be one of the an accurate positive replica of the oral structures, namely a most effective antimicrobial agents in the reduction of cast. The most important factors for a precise and successful plaque accumulation and the control of gingivitis, even 11 impression, regardless the impression material and the in lack of oral hygiene ; it also has an auxiliary effect in impression technique that was used are healthy soft and periodontal treatment through control of inflammation 12 hard tissues in the oral cavity. This can be achieved by and subgingival plaque accumulation . It is adsorbed the completion of necessary dental treatment, including in tooth surfaces, which is one of its most important periodontal, endodontic and orthodontic therapy2, and a high properties, even when these surfaces are covered with oral hygiene status. Interim prosthesis is called a fixed or hymenium, and it is released slowly, preventing microbe 10,13-15 removable dental or maxillofacial prosthesis, designed to proliferation . It shows low toxicity and infrequent enhance aesthetics, stabilization and/or function for a limited side effects, which appear mainly after long term use, such period of time, after which it is to be replaced by a definitive as brown discoloration of tooth surfaces and restorative materials16, alteration in taste perception11,17, an increase of prosthesis. In fixed prosthodontics, provisional restorations are 18 fabricated using various techniques3-6 and should fulfill given calculus formation , and soft tissue delamination and teeth biological, mechanical and aesthetic requirements. They can hypersensitivity; (4) C31G, a 0.3% solution in an aqueous either enhance the oral tissues health7 or may induce a slight base, which affects bacterium glycolysis and has wide 19; inflammatory response of the adjacent soft tissues. germicidal action against Gram + and Gram - bacteria According to Trushkowsky,6 provisional restorations in vitro, C31G in concentration of 0.5% is as effective as chlorhexidine20. It is chemically sticky and binds itself to should fulfil several requirements in order to avoid plaque sites within the periodontal pocket, so it continues to be accumulation and consequently periodontal inflammation: effective for prolonged periods of time. 1. They should isolate the prepared teeth from the oral The combined use of mechanical and chemical environment; means in oral hygiene is the most effective way to control 2. They should have an adequate anatomical shape, plaque accumulation and therefore keep the periodontal form and marginal fit; tissues healthy. The aim of this study was to evaluate the 3. They should be able to maintain the prepared teeth in efficacy of C31G on inhibiting inflammation around teeth position, providing contact points with adjacent teeth bearing provisional restorations prior to final impressions and antagonists. for fixed prostheses and compare its efficacy with that Even when provisional restorations are made of chlorhexidine and a placebo. Secondary objective of following the above mentioned standards, they can induce the study was to estimate the degree of discoloration on a mild inflammatory response at the surrounding soft provisional restorations21-23, as well as changes in taste tissues. This is usually due to the nature of the materials perception, caused by the use of C31G in comparison used and may affect the quality of final impressions. with chlorhexidine and placebo (mouthrinse without an Antimicrobial agents have an important effect to active agent). periodontal health, as microbial plaque is the major etiologic factor of periodontal disease8. Consequently, the reduction or even elimination of the perio-pathogenic microorganisms is the most significant part of the Materials and Methods periodontal therapy9. There are various antimicrobial agents that can be used, which can be divided in 5 This study was a randomized, double-blind clinical categories according to Mandel9 : trial. 28 consecutive patients participated, who presented 1. Antiseptics; for treatment in the Graduate Prosthodontics Clinic at the 2. Antibiotics; School of Dentistry of the University of Athens, Greece. 3. Enzymes; The inclusion criteria for the patients were as follows: 4. Non-enzymatic agents; ● Patients had to be at least 18 years old and participate 5. Agents able to destroy the adherence between in the trial voluntarily; some bacteria or between the bacteria and the tooth ● They had to have free medical history; surfaces. ● They should not have been on antibiotic or anti- The most known antimicrobial agents are: (1) Listerin inflammatory treatment; that reduces plaque accumulation by 35% and gingival ● They should not have been heavy smokers (not more inflammation10, although it doesn’t seem to have any than 8 cigarettes per day); Balk J Stom, Vol 15, 2011 Antimicrobial Mouthrinses for Treating Soft Tissues 135 ● They had to have completed their periodontal the antimicrobial solution. All antimicrobial agents were treatment; placed in identical bottles. ● Dimensions and location of the restorations to be placed should have been the same, as far as possible. Final Examination The patients were randomly assigned to 3 groups 4 weeks later, the same measurements were repeated according to the applied treatment, by means of a table of and information on possible side effects was accumulated. random numbers: Final impressions were made at the same appointment, for Group A. Patients received 0.3% solution of C31G the fabrication of permanent restorations. (Therasol®, Ora Tec Corp., Herndon, VA, USA); Group B. Patients received solution of chlorhexidine Statistical Analysis 0.12% (Peridex®, Procter and Gamble, Cincinnati, Ohio, USA); The experimental planning was done with Group C, (control group). Patients received a placebo. multivariate analysis of variance (MANOVA), with All patients were instructed to use their mouthrinse repeated