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S1 Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm
Sulfate Reducing Bacteria and Mycobacteria Dominate the Biofilm Communities in a Chloraminated Drinking Water Distribution System C. Kimloi Gomez-Smith 1,2 , Timothy M. LaPara 1, 3, Raymond M. Hozalski 1,3* 1Department of Civil, Environmental, and Geo- Engineering, University of Minnesota, Minneapolis, Minnesota 55455 United States 2Water Resources Sciences Graduate Program, University of Minnesota, St. Paul, Minnesota 55108, United States 3BioTechnology Institute, University of Minnesota, St. Paul, Minnesota 55108, United States Pages: 9 Figures: 2 Tables: 3 Inquiries to: Raymond M. Hozalski, Department of Civil, Environmental, and Geo- Engineering, 500 Pillsbury Drive SE, Minneapolis, MN 554555, Tel: (612) 626-9650. Fax: (612) 626-7750. E-mail: [email protected] S1 Table S1. Reference sequences used in the newly created alignment and taxonomy databases for hsp65 Illumina sequencing. Sequences were obtained from the National Center for Biotechnology Information Genbank database. Accession Accession Organism name Organism name Number Number Arthrobacter ureafaciens DQ007457 Mycobacterium koreense JF271827 Corynebacterium afermentans EF107157 Mycobacterium kubicae AY373458 Mycobacterium abscessus JX154122 Mycobacterium kumamotonense JX154126 Mycobacterium aemonae AM902964 Mycobacterium kyorinense JN974461 Mycobacterium africanum AF547803 Mycobacterium lacticola HM030495 Mycobacterium agri AY438080 Mycobacterium lacticola HM030495 Mycobacterium aichiense AJ310218 Mycobacterium lacus AY438090 Mycobacterium aichiense AF547804 Mycobacterium -
Role of the Laboratory in TB Diagnosis and Management
Role of the Laboratory in TB Diagnosis and Management Michael Pentella, Ph.D., D(ABMM), CIC Associate Director University Hygienic Lab Clinical Associate Professor, College of Public Health, University of Iowa Objectives • At the completion of this TB webinar, participants will: – Be familiar with the tests to diagnose latent tuberculosis and active tuberculosis – Recognize the tests available to detect Mycobacterium tuberculosis in clinical specimens – Understand the value of molecular tests to detect TB History of TB Diagnostics • Robert Koch announced in 1882 that he had found a microbe, Mycobacterium tuberculosis, that was the cause of "White Death", a disease responsible for one-seventh of all deaths in Europe in the late part of the 1800's. 1 Timeline of TB Infection Exposure 4-6 wks Latent Lifelong Adaptive Yrs-decades Containment T cell TB response (LTBI)* Active TB *Prevention efforts focus on detecting LTBI, most LTBI do not advance to active disease but those patients are at high risk particularly if they become immunocompromised. TB Infection vs. TB Disease TB in the body TB in the body Chest X-ray normal Chest X-ray abnormal Sputum not done Sputum smear and culture positive No symptoms Symptoms: cough, fever, weight loss Not infectious Infectious Not a case of TB Case of TB TB Algorithm • Collect sputum specimens at 3 different times and 8 hours apart (at least one must be a first morning specimen) for AFB smear and mycobacterial culture. • Perform MTD or NAAT test on the first smear positive sputum specimen 2 Diagnosis of -
Opportunist Mycobacteria
Thorax: first published as 10.1136/thx.44.6.449 on 1 June 1989. Downloaded from Thorax 1989;44:449454 Editorial Treatment of pulmonary disease caused by opportunist mycobacteria During the early 1950s it was recognised that recommended that surgical treatment for opportunist mycobacteria other than Mycobacterium tuberculosis mycobacterial infection should be given to those could cause pulmonary disease in man.' Over 30 years patients who are suitable surgical candidates.'7 The later there is no general agreement about the treatment failure of chemotherapy was often attributed to drug of patients with these mycobacterial infections. The resistance,'2 18 but the importance of prolonging the greatest controversy surrounds the treatment of in- duration of chemotherapy in opportunist mycobac- fection caused by the M aviwn-intracellulare- terial disease beyond that which would normally be scrofulaceum complex (MAIS), for which various required in tuberculosis was not appreciated. In treatments have been advocated, including chemo- several surgically treated series preoperative chemo- therapy with three23 or more' drugs or, alternatively, therapy was given on average for only four to seven surgical resection of the affected lung.78 Although the months,'3 19-22 some patients receiving as little as eight treatment of infection caused by M kansasii is less weeks of treatment before surgery.2324 In contrast, controversial there is no uniform approach to treat- chemotherapy alone, with isoniazid, p-aminosalicyclic ment. Disease caused by M xenopi has been described acid, and streptomycin for 24 months, produced by some as easy to treat with chemotherapy,9 whereas successful results in 80-100% of patients with M others have found the response to drug treatment to be kansasii infection despite reports of in vitro resistance unpredictable.'' " to these agents.2125 This diversity ofopinion and approach to treatment has arisen for two reasons. -
Species of Mycobacterium Tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens from Serbia
Arch. Biol. Sci., Belgrade, 66 (2), 553-561, 2014 DOI:10.2298/ABS1402553Z SPECIES OF MYCOBACTERIUM TUBERCULOSIS COMPLEX AND NONTUBERCULOUS MYCOBACTERIA IN RESPIRATORY SPECIMENS FROM SERBIA IRENA ŽIVANOVIĆ1, DRAGANA VUKOVIĆ1, IVANA DAKIĆ1 and BRANISLAVA SAVIĆ1 1 Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia Abstract - This study aimed to provide the first comprehensive report into the local pattern of mycobacterial isolation. We used the GenoType MTBC and CM/AS assays (Hain Lifescience) to perform speciation of 1 096 mycobacterial cultures isolated from respiratory specimens, one culture per patient, in Serbia over a 12-month period. The only species of the Mycobacterium tuberculosis complex (MTBC) identified in our study was M. tuberculosis, with an isolation rate of 88.8%. Ten different species of nontuberculous mycobacteria (NTM) were recognized, and the five most frequently isolated spe- cies were, in descending order, M. xenopi, M. peregrinum, M. gordonae, M. avium and M. chelonae. In total, NTM isolates accounted for 11.2% of all isolates of mycobacteria identified in pulmonary specimens. Our results suggest that routine differentiation among members of the MTBC is not necessary, while routine speciation of NTM is required. Key words: Mycobacterium tuberculosis, nontuberculous mycobacteria, identification, GenoType MTBC, GenoType CM/AS INTRODUCTION M. mungi in banded mongooses (Alexander et al., 2010), and M. orygis in animals of the Bovidae family Currently, the genus Mycobacterium encompasses (van Ingen et al., 2012) have recently been described. 163 species and 13 subspecies described in the list of Although all members of the complex are considered bacterial species with approved names (www.bacte- tubercle bacilli, the most important causative agent rio.cict.fr/m/mycobacterium.html). -
Mycobacterium Goodii Endocarditis Following Mitral Valve Ring Annuloplasty Rohan B
Parikh and Grant Ann Clin Microbiol Antimicrob (2017) 16:14 DOI 10.1186/s12941-017-0190-4 Annals of Clinical Microbiology and Antimicrobials CASE REPORT Open Access Mycobacterium goodii endocarditis following mitral valve ring annuloplasty Rohan B. Parikh1 and Matthew Grant2* Abstract Background: Mycobacterium goodii is an infrequent human pathogen which has been implicated in prosthesis related infections and penetrating injuries. It is often initially misidentified as a gram-positive rod by clinical microbio- logic laboratories and should be considered in the differential diagnosis. Case presentation: We describe here the second reported case of M. goodii endocarditis. Species level identification was performed by 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing. The patient was successfully treated with mitral valve replacement and a prolonged combination of ciprofloxacin and trimethoprim/sulfamethoxazole. Conclusion: Confirmation of the diagnosis utilizing molecular techniques and drug susceptibility testing allowed for successful treatment of this prosthetic infection. Keywords: Mycobacterium goodii, Endocarditis, Gene sequencing, Prostheses related infections Background appreciated at the apex, and a drain was in place for a Mycobacterium goodii is a rapidly growing non-tubercu- groin seroma related to recent left heart catheterization. lous mycobacterium (NTM) belonging to the Mycobac- He had an unsteady gait and exhibited mild left lower terium smegmatis [1] group. Its importance has become extremity weakness (4/5). His brain magnetic resonance increasingly appreciated as a pathogen over the last imaging showed multiple ring-enhancing lesions in the 20 years, with a predilection towards infecting tissues at pons and posterior fossa suggestive of septic emboli. the site of penetrating injuries. Antibacterial treatment Transthoracic echocardiography showed moderate strategies against this pathogen are diverse but reported mitral regurgitation without any vegetation. -
An Abstract of the Dissertation of Melanie J. Harriff
AN ABSTRACT OF THE DISSERTATION OF MELANIE J. HARRIFF for the degree of Doctor of Philosophy in Molecular and Cellular Biology presented on June 8, 2007. Title: Mechanisms for the Interaction of Environmental Mycobacteria with Host Cells Abstract approved: Luiz E. Bermudez Michael L. Kent Environmental mycobacteria are important opportunistic pathogens for many hosts, including humans, cattle, and fish. Two well-studied species are Mycobacterium avium subsp. avium, a significant cause of disseminated bacterial disease in patients with AIDS, and Mycobacterium avium subsp. paratuberculosis, the cause of Johne’s disease in cattle. Many other species that are considerable sources of infections in fish, such as Mycobacterium chelonae and Mycobacterium marinum, also have zoonotic potential. To gain knowledge about the invasion of epithelial cells by environmental mycobacteria, selected genes and proteins involved in the uptake of M. avium by HEp-2 cells were analyzed by a variety of methods. Two transcriptional regulators (MAV_3679 and MAV_5138) were identified as being involved in invasion. A mycobacterial protein (CipA) with an amino acid sequence suggestive of an ability to be a part of the scaffolding complex that forms during cell signaling leading to actin polymerization was found to putatively interact with host cell Cdc42. Fusion of CipA to GFP, expressed in Mycobacterium smegmatis, revealed that CipA localizes to a structure on the surface of bacteria approaching HEp-2 cells. To establish whether species of environmental mycobacteria isolated from different hosts use similar mechanisms to M. avium for interaction with the mucosa, and for survival in macrophages, assays to determine invasion and replication were performed in different cell types, and a custom DNA microarray containing probes for known mycobacterial virulence determinants was developed. -
Genome Sequencing of Mycobacterium Pinnipedii Strains
Silva-Pereira et al. BMC Genomics (2019) 20:1030 https://doi.org/10.1186/s12864-019-6407-5 RESEARCH ARTICLE Open Access Genome sequencing of Mycobacterium pinnipedii strains: genetic characterization and evidence of superinfection in a South American sea lion (Otaria flavescens) Taiana T. Silva-Pereira1,2, Cássia Y. Ikuta2, Cristina K. Zimpel1,2, Naila C. S. Camargo1,2, Antônio F. de Souza Filho2, José S. Ferreira Neto2, Marcos B. Heinemann2 and Ana M. S. Guimarães1,2* Abstract Background: Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis Complex (MTBC), is capable of infecting several host species, including humans. Recently, ancient DNA from this organism was recovered from pre-Columbian mummies of Peru, sparking debate over the origin and frequency of tuberculosis in the Americas prior to European colonization. Results: We present the first comparative genomic study of this bacterial species, starting from the genome sequencing of two M. pinnipedii isolates (MP1 and MP2) obtained from different organs of a stranded South American sea lion. Our results indicate that MP1 and MP2 differ by 113 SNPs (single nucleotide polymorphisms) and 46 indels, constituting the first report of a mixed-strain infection in a sea lion. SNP annotation analyses indicate that genes of the VapBC family, a toxin-antitoxin system, and genes related to cell wall remodeling are under evolutionary pressure for protein sequence change in these strains. OrthoMCL analysis with seven modern isolates of M. pinnipedii shows that these strains have highly similar proteomes. Gene variations were only marginally associated with hypothetical proteins and PE/PPE (proline-glutamate and proline-proline-glutamate, respectively) gene families. -
RAPIDLY GROWING, ACID FAST BACTERIA' Original 21 of This Species
RAPIDLY GROWING, ACID FAST BACTERIA' II. SPEcIES' DESCRPTION OF Mycobacteriumfortuitum CRUZ RUTH E. GORDON AND MILDRED M. SMITH Institute of Microbiology, Rutgers University, the State University of New Jersey, New Brunswick, New Jersey Received for publication October 13, 1954 The taxonomic study of the acid fast bacteria the following medium, a modification of Koser's capable of comparatively rapid growth on citrate agar (1924): NaCl, 1 g; MgSO4, 0.2 g; ordinary media, first reported in 1953 by Gordon (NH4)2HP04, 1 g; KH2PO4, 0.5 g; Na benzoate, and Smith, has been continued. Additional 2 g; agar, 15 g; distilled water, 1,000 ml. The strains have been examined and other tests ap- pH of the agar was adjusted to 7.0, and 20 ml plied to all the strains. A few supplementary of a 0.04 per cent solution of phenol red were characteristics of the two previously delineated added. An alkaline reaction of the medium in- species, Mycobacterium phlei Lehmann and dicated use of the benzoate. Neumanm and Mycobacterium smgmatis (Trevi- Acid from carbohydrats. Maltose and trehalose san) Lehmann and Neumann, are presented, and were used in conjunction with the carbohydrates the strains newly assigned to these species are previously listed. listed. As the work progresed, a third group of strains DESCRIPONS OF SPECIES emerged. The strains of this taxon seemed The collection2 of mycobacteria forming the closely related to each other and sufficiently basis of this taxonomic study increased from distinct from the other strains of the collection 124 of first to 195. The to warrant their separation into a species. -
Zoonotic Tuberculosis in Mammals, Including Bovine and Caprine
Zoonotic Importance Several closely related bacteria in the Mycobacterium tuberculosis complex Tuberculosis in cause tuberculosis in mammals. Each organism is adapted to one or more hosts, but can also cause disease in other species. The two agents usually found in domestic Mammals, animals are M. bovis, which causes bovine tuberculosis, and M. caprae, which is adapted to goats but also circulates in some cattle herds. Both cause economic losses including in livestock from deaths, disease, lost productivity and trade restrictions. They can also affect other animals including pets, zoo animals and free-living wildlife. M. bovis Bovine and is reported to cause serious issues in some wildlife, such as lions (Panthera leo) in Caprine Africa or endangered Iberian lynx (Lynx pardinus). Three organisms that circulate in wildlife, M. pinnipedii, M. orygis and M. microti, are found occasionally in livestock, Tuberculosis pets and people. In the past, M. bovis was an important cause of tuberculosis in humans worldwide. It was especially common in children who drank unpasteurized milk. The Infections caused by advent of pasteurization, followed by the establishment of control programs in cattle, Mycobacterium bovis, have made clinical cases uncommon in many countries. Nevertheless, this disease is M. caprae, M. pinnipedii, still a concern: it remains an important zoonosis in some impoverished nations, while wildlife reservoirs can prevent complete eradication in developed countries. M. M. orygis and M. microti caprae has also emerged as an issue in some areas. This organism is now responsible for a significant percentage of the human tuberculosis cases in some European countries where M. bovis has been controlled. -
Nontuberculous Mycobacteria in Respiratory Samples from Patients with Pulmonary Tuberculosis in the State of Rondônia, Brazil
Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 108(4): 457-462, June 2013 457 Nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of Rondônia, Brazil Cleoni Alves Mendes de Lima1,2/+, Harrison Magdinier Gomes3, Maraníbia Aparecida Cardoso Oelemann3, Jesus Pais Ramos4, Paulo Cezar Caldas4, Carlos Eduardo Dias Campos4, Márcia Aparecida da Silva Pereira3, Fátima Fandinho Onofre Montes4, Maria do Socorro Calixto de Oliveira1, Philip Noel Suffys3, Maria Manuela da Fonseca Moura1 1Centro Interdepartamental de Biologia Experimental e Biotecnologia, Universidade Federal de Rondônia, Porto Velho, RO, Brasil 2Laboratório Central de Saúde Pública de Rondônia, Porto Velho, RO, Brasil 3Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz 4Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, RJ, Brasil The main cause of pulmonary tuberculosis (TB) is infection with Mycobacterium tuberculosis (MTB). We aimed to evaluate the contribution of nontuberculous mycobacteria (NTM) to pulmonary disease in patients from the state of Rondônia using respiratory samples and epidemiological data from TB cases. Mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. Among the 1,812 cases suspected of having pulmonary TB, 444 yielded bacterial cultures, including 369 cases positive for MTB and 75 cases positive for NTM. Within the latter group, 14 species were identified as Mycobacterium abscessus, Mycobacterium avium, Mycobacterium fortuitum, Myco- bacterium intracellulare, Mycobacterium gilvum, Mycobacterium gordonae, Mycobacterium asiaticum, Mycobac- terium tusciae, Mycobacterium porcinum, Mycobacterium novocastrense, Mycobacterium simiae, Mycobacterium szulgai, Mycobacterium phlei and Mycobacterium holsaticum and 13 isolates could not be identified at the species level. -
Mycobacteria and Disease in Southern Africa L
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Stellenbosch University SUNScholar Repository Transboundary and Emerging Diseases REVIEW ARTICLE Mycobacteria and Disease in Southern Africa L. Botha, N. C. Gey van Pittius and P. D. van Helden DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/Medical Research Council (MRC) Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa Keywords: Summary Mycobacterium tuberculosis complex; M. bovis; NTM; southern Africa The genus Mycobacterium consists of over 120 known species, some of which (e.g. M. bovis and M. tuberculosis) contribute extensively to the burden of infec- Correspondence: tious disease in humans and animals, whilst others are commonly found in the P. D. van Helden, DST/NRF Centre of environment but may rarely if ever be disease-causing. This paper reviews the Excellence for Biomedical Tuberculosis mycobacteria found in southern Africa, focussing on those in the M. tuberculosis Research/ Medical Research Council (MRC) complex as well as the non-tuberculous mycobacteria (NTM), identifying those Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human found in the area and including those causing disease in humans and animals, Genetics, Faculty of Health Sciences, and outlines some recent reports describing the distribution and prevalence of Stellenbosch University, PO Box 19063, the disease in Africa. Difficulties in diagnosis, host preference and reaction, Tygerberg 7505, South Africa. immunology and transmission are discussed. Tel.: +21 938 9124; Fax: +21 938 9863; E-mail: [email protected] Received for publication August 1, 2013 doi:10.1111/tbed.12159 slow-growing species (more than 7 days in culture) are Introduction mostly pathogenic (see Fig. -
Identification of Mycobacterium Avium Pathogenicity Island Important For
MICROBIOLOGY. For the article ‘‘Identification of Mycobacterium avium pathogenicity island important for macrophage and amoeba infection,’’ by Lia Danelishvili, Martin Wu, Bernadette Stang, Melanie Harriff, Stuart Cirillo, Jeffrey Cirillo, Robert Bildfell, Brian Arbogast, and Luiz E. Bermudez, which appeared in issue 26, June 26, 2007, of Proc Natl Acad Sci USA (104:11038– 11043; first published June 19, 2007; 10.1073͞pnas.0610746104), the author name Stuart Cirillo should have appeared as Suat L. G. Cirillo, and the author name Jeffrey Cirillo should have appeared as Jeffrey D. Cirillo. The online version has been corrected. The corrected author line appears below. Addition- ally, the present address for both these authors should be: Department of Microbial and Molecular Pathogenesis, Texas A&M University College of Medicine, College Station, TX 77843-1114. The authors also note that Fig. 1 did not print at high resolution. The corrected figure and its legend appear below. Lia Danelishvili, Martin Wu, Bernadette Stang, Melanie Harriff, Suat L. G. Cirillo, Jeffrey D. Cirillo, Robert Bildfell, Brian Arbogast, and Luiz E. Bermudez Fig. 1. Chromosome regions. (A) Organization of the chromosome region inactivated in the 8H8 clone of M. avium involved in the glycosylation of the lipopeptide core. (B) Organization of the chromosome region inactivated in the M. avium 9B9 clone. The M. avium gene names correspond to MAP numbers from the M. avium subsp. paratuberculosis genome sequence. (C) Genetic organization of M. avium 104 PI associated with low invasion of macrophages and virulence in mice. The M. avium 104 (b) sequence and gene organization of this region are presented in comparison with M.