DOCSLIB.ORG

Recovery of Pdike2 Plasmid for Hepatitis C Vaccine Using Cimmultus® C4 HLD Monolith

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Recovery of Pdike2 Plasmid for Hepatitis C Vaccine Using Cimmultus® C4 HLD Monolith Application Note September 4, 2014 Keywords or phrases: pDNA, CIMmultus® C4 HLD, CIMac™ pDNA, chromatography, monoliths Recovery of pDIKE2 Plasmid for Hepatitis C Vaccine Using CIMmultus® C4 HLD Monolith BIA Separations d.o.o., A Sartorius company, Mirce 21, 5270 Ajdovščina, Slovenia Correspondence E-Mail: [email protected] Abstract pDNA plays a crucial role in modern healthcare, specifically in the gene therapy field. Its main application is as the delivery vehicle, or vector, to introduce foreign DNA into organisms. Monolithic columns can be used in the production of pDNA as raw material or therapeutic products. This application note shows the results of pDNA purification with the CIMmultus® C4 HLD column. For More Information, Visit www.sartorius.com/monoliths Introduction Methods DNA immunization can potentially induce both humoral After size exclusion chromatography the pDNA fraction and cellular immune responses, making it an attractive eluted was applied on CIMmultus® C4 HLD-800 Advanced approach to developing an effective vaccine against HCV. Composite Column cGMP, finally the elution was The pIDKE2 plasmid is the main component of the CIGB’s concentrated until 2 mg/mL and filtered at 0.2 μm. candidate vaccine against Hepatitis C virus (HVC), which is used to treat HCV chronically-infected individuals during Column: CIMmultus® C4 HLD 800 mL cGMP Compliant clinical trials phase 1 and 2. Monolithic Column (HLD Butyl) An improvement to the downstream process was required Mobile phases Buffer A: 1.75 M (NH₄)₂SO₄, 0.025 M Tris; to produce the necessary quantities to meet the high 0.010 EDTA pH7 and Buffer B: 0.025 M Tris; demand for plasmids for clinical trials. 0.010 EDTA pH7 Flow rate 300 mL/min Equlibration 100% Buffer A, washing after sample loading: of column 100% Buffer A, elution: 100 % Buffer B Load 2.6 L Detection λ = 254 nm Materials System Sepacore System from Buchi CIMmultus® C4 HLD 800 mL cGMP Compliant Monolithic ] Column (HLD Butyl) 70 mAU [ 60 50 Absorbance Absorbance 40 30 20 10 0 0 5 10 15 20 25 30 35 40 Time [min] Figure 1: Chromatographic behavior of CIMmultus® C4 HLD 800 mL column, cGMP 2 Purification Scheme Column: CIMac™ pDNA-0.3 Analytical Column Mobile phases Buffer A: Buffer A: 200 mM Tris + 0.6 M NaCl, Biomass pH 8.0 and Buffer B: 200 mM Tris + 0.7 M NaCl, pH 8.0 Flow rate 1 mL/min Step gradient A linear gradient from 0 to 100% Buffer B in 10 min. Cell disruption method Load 2.6 L Detection λ = 254 nm TFF System HPLC Merck - Hitachi Size exclusion chromatography ] 140 mAU [ 120 7.85 CIMmultus® C4 HLD-800 Intensity Intensity 100 80 TFF 60 40 Final Filtration 20 0.29 6.70 10.29 Final Product 0 0 2 4 6 8 10 12 14 16 Retention Time [min] Figure 2: Elution of CIMmultus® C4 HLD fraction analyzed on CIMac™ pDNA-0.3 Analytical Column Results Purified pDNA contains more than 95% purity of pIDKE2. The content of genomic DNA is lower than 5 μg per dose, RNA is not detectable by agarose gel electrophoresis; en- dotoxin content is below 5.0 EU per kg body weight, and the protein content is 1.4 μg per dose, which is lower than the limit established. 3 Germany USA Slovenia Sartorius Stedim Biotech GmbH Sartorius Stedim North America Inc. BIA Separations d.o.o. August-Spindler-Straße 11 565 Johnson Avenue A Sartorius company 37079 Göttingen Bohemia, NY 11716 Mirce 21 Phone +49 551 308 0 Toll-Free +1 800 368 7178 5270 Ajdovščina For More Information, Visit www.sartorius.com Specifications subject to change without notice. © 2021 Copyright Sartorius Stedim Biotech GmbH, August-Spindler-Strasse 11, 37079 Goettingen, Germany Status: 04 | 29 | 2021.
Load more

Recommended publications
  • Purification of Recombinant Vaccinia Virus for Oncolytic and Immunotherapeutic Applications Using Monolithic Column Technology
    Purification of Recombinant Vaccinia Virus for Oncolytic and Immunotherapeutic Applications using Monolithic Column Technology A thesis submitted to University College London for the degree of Doctor of Engineering by David Isaac William Vincent The Advanced Centre for Biochemical Engineering Department of Biochemical Engineering University College London Gordon Street London WC1H 0AH 1 I, David Vincent confirm that the work presented in this thesis is my own. Where information has been derived from other sources, I confirm that this has been indicated in the thesis. Signed……………….………………………Date……………………………………… 2 Acknowledgements I would like to thank my supervisor Dr Yuhong Zhou for her invaluable feedback and support over the course of this EngD. Without her continuous encouragement this work would likely never have been completed. I would also like to thank my Advisor Prof. Ajoy Velayudhan for his help and guidance. Many a long discussion was had on the intricacies of vaccinia viral purification which were thought provoking, inspirational but also really good fun. I would also like to express my gratitude to the staff and students at UCL. There are too many to mention everyone, but in particular I would like to thank Mrs Ludmila Ruban, Miss Dhushy Stanislaus and Dr Tarit Mukhopadhyay for their support, especially in setting up the vaccine lab in the department which I could benefit from in my final year. I would also like to thank Dr Patricia Perez Esteban for her support, friendship and bioprocess wisdom. I would like to express my gratitude to BIA Separations and the EPSRC for sponsoring this work. Without this financial support this work would not have been possible.
    [Show full text]
  • BIA Separations CIM Monolithic Columns for Purification and Analytics of Biomolecules
    BIA Separations CIM Monolithic Columns for Purification and Analytics of Biomolecules Lidija Urbas, PhD [email protected] Outline • BIA Separations • Chromatography – Monolithic Chromatography – Design of monolithic columns • DSP applications • PAT columns and applications: – Case study: combining DSP and PAT of Adenoviruses • Conclusions Ajdovščina BIA Separations • BIA Separations was founded in September 1998. • Headquarters in Austria, R&D and Production in Slovenia. BIA Separations • BIA Separations was founded in September 1998. • Headquarters in Austria, R&D and Production in Slovenia. • BIA Separations USA established in September 2007 - sales and tech support office. • BIA Separations China established in January 2011 - sales and tech support office. • 90 employees world wide • Main focus: To develop and sell methacrylate monolithic columns & develop methods and processes for large biomolecules separation and purification. BIA Separations – Products and Services CIM Contract monolithic Research columns Laboratory Method development and Technical Support Important Milestones • 2004: First monolith used for the industrial cGMP purification for plasmid DNA at Boehringer Ingelheim provide 15-fold increase in productivity • 2006: First cGMP production of a vaccine (influenza) using CIM® • 2008: OEM Partnership with Agilent Technologies – develop and produce analytical monolithic columns for PAT • In 2011 BIA Separations was awarded by KAPPA-Health as a model SME in the EU Co-funded research projects • 2012: co-marketing and
    [Show full text]
  • Simian Adenovirus Vector Production for Early-Phase Clinical Trials: A
    Vaccine 37 (2019) 6951–6961 Contents lists available at ScienceDirect Vaccine journal homepage: www.elsevier.com/locate/vaccine Simian adenovirus vector production for early-phase clinical trials: A simple method applicable to multiple serotypes and using entirely disposable product-contact components Sofiya Fedosyuk a, Thomas Merritt b, Marco Polo Peralta-Alvarez a, Susan J Morris a, Ada Lam c, Nicolas Laroudie d, Anilkumar Kangokar c, Daniel Wright a, George M Warimwe e,f, Phillip Angell-Manning b, ⇑ Adam J Ritchie a, Sarah C Gilbert a, Alex Xenopoulos g, Anissa Boumlic d, Alexander D Douglas a, a Jenner Institute, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK b Clinical Biomanufacturing Facility, University of Oxford, Roosevelt Drive, Oxford OX3 7JT, UK c Millipore (UK) Ltd. Bedfont Cross, Stanwell Road, TW14 8NX Feltham, UK d Millipore SAS, 39 Route Industrielle de la Hardt, Molsheim 67120, France e Centre for Tropical Medicine and Global Health, University of Oxford, Roosevelt Drive, Oxford OX3 7FZ, UK f KEMRI-Wellcome Trust Research Programme, P.O. 230-80108 Kilifi, Kenya g EMD Millipore Corporation, 80 Ashby Road, Bedford, MA 01730, USA article info abstract Article history: A variety of Good Manufacturing Practice (GMP) compliant processes have been reported for production Available online 30 April 2019 of non-replicating adenovirus vectors, but important challenges remain. Most clinical development of adenovirus vectors now uses simian adenoviruses or rare human serotypes, whereas reported manufac- Keywords: turing processes mainly use serotypes such as AdHu5 which are of questionable relevance for clinical Simian adenovirus vaccine development. Many clinically relevant vaccine transgenes interfere with adenovirus replication, GMP whereas most reported process development uses selected antigens or even model transgenes such as Clinical trials fluorescent proteins which cause little such interference.
    [Show full text]
  • Monolithic Columns for Purification and In-Process Control of Viruses and Virus-Like Particles" in "Vaccine Technology IV", B
    Engineering Conferences International ECI Digital Archives Vaccine Technology IV Proceedings Spring 5-24-2012 Monolithic Columns for Purification and In- process Control of Viruses and Virus-like Particles Lidija Urbas BIA Separations Milos Barut BIA Separations Follow this and additional works at: http://dc.engconfintl.org/vaccine_iv Part of the Biomedical Engineering and Bioengineering Commons Recommended Citation Lidija Urbas and Milos Barut, "Monolithic Columns for Purification and In-process Control of Viruses and Virus-like Particles" in "Vaccine Technology IV", B. Buckland, University College London, UK; J. Aunins, Janis Biologics, LLC; P. Alves , ITQB/IBET; K. Jansen, Wyeth Vaccine Research Eds, ECI Symposium Series, (2013). http://dc.engconfintl.org/vaccine_iv/43 This Conference Proceeding is brought to you for free and open access by the Proceedings at ECI Digital Archives. It has been accepted for inclusion in Vaccine Technology IV by an authorized administrator of ECI Digital Archives. For more information, please contact [email protected]. Monolithic Columns for Purification and In-process Control of Viruses and Virus-like Particles Lidija Urbas, Miloš Barut Faro, May 24th 2012 Outline • Conventional particle based chromatography • Monolith based chromatography • Downstream processing of Ad3 VLPs – Case study • Convective interaction media (CIM) monoliths for in-process control – CIMac monolithic analytical columns as a tool for HPLC based analytical methods BIA Separations • BIA Separations was founded in September 1998. • Headquartes in Austria, R&D and Production in Slovenia. • BIA Separations USA established in September 2007 - sales and tech support office. • BIA Separations China established in January 2011 - sales and tech support office. • Main focus: To develop and sell methacrylate monolithic columns & develop methods and processes for large biomolecules separation and purification .
    [Show full text]
  • Downstream Processing of Cell Culture-Derived Animal Viruses
    Downstream processing of cell culture-derived animal viruses Dina de Jesus Marinheiro Antunes Thesis to obtain the Master of Science Degree in Biotechnology Supervisors: Prof. Maria Raquel Murias dos Santos Aires Barros Dr. Miguel Agostinho Sousa Pinto de Torres Fevereiro Examination Committee Chairperson: Prof. Luís Joaquim Pina da Fonseca Supervisor: Dr. Miguel Agostinho Sousa Pinto de Torres Fevereiro Member of the Committee: Dr. Ana Margarida Ferreira Henriques de Oliveira Mourão July, 2014 2 Dedicated to Querida Mãe e Pai (Dear Mom and Dad) "Nós nunca nos realizamos. Somos dois abismos - um poço fitando o céu." Fernando Pessoa 3 Acknowledgments All my friends say that one of my major defects, which I totally disagree (I am a little bit stubborn…), is saying “Obrigada” (Thank you) too often. This word has its origin from the latim obligatus, participle of the verb obligare, which means connect, attach. When we mention it, we feel connected, even for a moment, to some favor someone did. And I am glad to have a section in my Dissertation where I can finally say this beautiful word how many times I want. And tie me to the people. “Obrigada” This dissertation would not have been possible without the guidance and cooperation of several people who contributed with their invaluable assistance in the formulation, development and completion of this study. First and foremost, I would like to show my greatest gratitude to Dr. Miguel Fevereiro, who provided an inspiring environment to work in and taught me the real sense of “negative thinking”. He showed that it is possible (believe me!) to be an exceptional Director, teacher, researcher and vet, with a LOT of work, love and engagement.
    [Show full text]
  • DNA Hydrolysing Igg Catalytic Antibodies: an Emerging Link Between Psychoses and Autoimmunity
    www.nature.com/npjschz ARTICLE OPEN DNA hydrolysing IgG catalytic antibodies: an emerging link between psychoses and autoimmunity Rajendran Ramesh1, Aparna Sundaresh2, Ravi Philip Rajkumar3, Vir Singh Negi2, M. A. Vijayalakshmi1, Rajagopal Krishnamoorthy4, ✉ Ryad Tamouza4,5,6,7, Marion Leboyer4,5,6,7 and A. S. Kamalanathan 1 It is not uncommon to observe autoimmune comorbidities in a significant subset of patients with psychotic disorders, namely schizophrenia (SCZ) and bipolar disorder (BPD). To understand the autoimmune basis, the DNA abyzme activity mediated by serum polyclonal IgG Abs were examined in psychoses patients, quantitatively, by an in-house optimized DNase assay. A similar activity exhibited by IgG Abs from neuropsychiatric-systemic lupus erythematosus (NP-SLE) patients was used as a comparator. Our data revealed that the IgG DNase activity of SCZ was close to that of NP-SLE and it was twofold higher than the healthy controls. Interestingly, the association between DNase activity with PANSS (positive, general and total scores) and MADRS were noted in a subgroup of SCZ and BPD patients, respectively. In our study group, the levels of IL-6 and total IgG in BPD patients were higher than SCZ and healthy controls, indicating a relatively inflammatory nature in BPD, while autoimmune comorbidity was mainly observed in SCZ patients. npj Schizophrenia (2021) 7:13 ; https://doi.org/10.1038/s41537-021-00143-6 1234567890():,; INTRODUCTION revealed a high prevalence of anti-NMDAR IgG Abs in SCZ 17 Growing evidence indicate that neuroinflammation and immune patients and to a lesser extent in BPD patients . dysfunction play a role in the clinical manifestations of psychotic Above facts suggest a potential overlap between psychotic disorders viz.
    [Show full text]
  • Comparison of Different Liquid Chromatography-Based Purification
    pharmaceutics Article Comparison of Different Liquid Chromatography-Based Purification Strategies for Adeno-Associated Virus Vectors Ruth Rieser 1, Johanna Koch 1, Greta Faccioli 1 , Klaus Richter 2, Tim Menzen 2 , Martin Biel 1, Gerhard Winter 1,* and Stylianos Michalakis 1,3,* 1 Department of Pharmacy—Center for Drug Research, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377 Munich, Germany; [email protected] (R.R.); [email protected] (J.K.); [email protected] (G.F.); [email protected] (M.B.) 2 Coriolis Pharma, Fraunhoferstr. 18 b, 82152 Martinsried, Germany; [email protected] (K.R.); [email protected] (T.M.) 3 Department of Ophthalmology, University Hospital, LMU Munich, Mathildenstr. 8, 80336 Munich, Germany * Correspondence: [email protected] (G.W.); [email protected] (S.M.) Abstract: Recombinant adeno-associated virus (rAAV) vectors have evolved as one of the most promising technologies for gene therapy due to their good safety profile, high transduction efficacy, and long-term gene expression in nondividing cells. rAAV-based gene therapy holds great promise for treating genetic disorders like inherited blindness, muscular atrophy, or bleeding disorders. There is a high demand for efficient and scalable production and purification methods for rAAVs. This is particularly true for the downstream purification methods. The current standard methods are based on multiple steps of gradient ultracentrifugation, which allow for the purification and enrichment of Citation: Rieser, R.; Koch, J.; Faccioli, full rAAV particles, but the scale up of this method is challenging. Here, we explored fast, scalable, G.; Richter, K.; Menzen, T.; Biel, M.; and universal liquid chromatography-based strategies for the purification of rAAVs.
    [Show full text]
  • The Separation of Viruses from One Another
    Journal of Chromatography A, 1388 (2015) 69–78 Contents lists available at ScienceDirect Journal of Chromatography A j ournal homepage: www.elsevier.com/locate/chroma A new application of monolithic supports: The separation of viruses from one another a,∗ b b a a J. Rusˇciˇ c´ , I. Gutiérrez-Aguirre , M. Tusekˇ Znidariˇ cˇ , S. Kolundzijaˇ , A. Slana , c,1 b a M. Barut , M. Ravnikar , M. Krajaciˇ c´ a Department of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia b Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia c BIA Separations d.o.o., Ajdovsˇcina,ˇ Slovenia a r t i c l e i n f o a b s t r a c t Article history: The emergence of next-generation “deep” sequencing has enabled the study of virus populations with Received 13 November 2014 much higher resolutions. This new tool increases the possibility of observing mixed infections caused by Received in revised form 30 January 2015 combinations of plant viruses, which are likely to occur more frequently than previously thought. The bio- Accepted 30 January 2015 logical impact of co-infecting viruses on their host has yet to be determined and fully understood, and the Available online 9 February 2015 first step towards reaching this goal is the separation and purification of individual species. Ion-exchange monolith chromatography has been used successfully for the purification and concentration of different Keywords: viruses, and number of them have been separated from plant homogenate or bacterial and eukaryotic Mixed infections lysate. Thus, the question remained as to whether different virus species present in a single sample could Virus strains be separated.
    [Show full text]
  • Monolith Absorbants As a Capture Step for Virus-Like
    MONOLITH ABSORBANTS AS A CAPTURE STEP FOR VIRUS-LIKE PARTICLES CLAIRE SUZANNE BURDEN Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London. WC1E 7JE Submitted to UCL for the degree of Doctor of Engineering (EngD) in Biochemical Engineering and Bioprocess Leadership September 2012 1 I, Claire Burden, confirm that the work presented in this thesis is my own. Where information has been derived from other sources, I confirm that this has been indicated in the thesis. Signed…………………………………………………………..Date……………… 2 ABSTRACT Monoliths are an alternative stationary phase format to conventional particle based media for large biomolecules. Conventional resins suffer from limited capacities and flow rates when used for viruses, virus-like particles (VLP) and other nanoplex materials. Monoliths provide an open pore structure to improve pressure drops and mass transport via convective flow. The challenging capture of a VLP from clarified yeast homogenate was used to develop a new monolith separation which found hydrophobic interaction based separation using a hydroxyl derivatised monolith had the best performance. The monolith was then compared to a known beaded resin method, where the dynamic binding capacity increased three-fold for the monolith with 90% recovery of the VLP. Confocal microscopy was used to visualise lipid contaminants, deriving from the homogenised yeast. The lipid formed a layer on top of the column, even after column regeneration, resulting in increasing pressure drops over a number of cycles. Removal of 70% of the lipid pre-column by Amberlite/XAD-4 beads significantly reduced the fouling process. Applying a reduced lipid feed versus an untreated feed further increased the dynamic binding capacity of the monolith from 0.11 mg/mL column to 0.25mg/mL column.
    [Show full text]
  • Product Sheet & Instruction Manual
    BIA Separations d.o.o. CIM Convective Interaction Media® PRODUCT SHEET & INSTRUCTION MANUAL CIMac™ r-Protein L 0.1 mL Analytical Column (Recombinant Protein L) (1.3 µm channels) Product description Catalog number: 110.1021-1.3 CIMac™ Analytical Monoliths are high performance chromatography devices for rapid high- resolution fractionation of complex biological samples. The stationary phase is polymerised as a monolith with homogeneous channel size and surface chemistry. Each unit is mounted in a precision engineered stainless steel housing to allow easy connection to any HPLC system. CIMac™ r-Protein L Analytical Columns are primarily intended for fast, efficient, and reproducible analysis of a wide range of antibodies and antibody fragments such as Fabs, single-chain variable fragments (scFv) and domain antibodies (Dabs). They can be used for quantitative and qualitative in-process and final control. The following information is provided to ensure proper product care and optimal product performance. Characteristics of the monolith Column chemistry: r-Protein L (affinity; recombinant protein L produced from E. Coli coupled to CDI-preactivated support) Colour code: White and red Channel radius: 675 nm (600 nm – 750 nm) Support matrix: Poly(glycidyl methacrylate -co- ethylene dimethacrylate) Monolith dimensions: Diameter: 5.2 mm; length: 4.95 mm; bed volume (CV): 0.1 mL Connector: 10-32 UNF coned port, 1/16’’ OD tubing connection Ligand density: N.D. Dynamic binding capacity: N.D. Operating flow rates: 0.2 – 3 mL/min (1 – 15 cm/min; 2 – 30 CV/min) Maximum pressure: 15 MPa, 150 bar, 2175 psi Operating temperature: 4 °C (39 °F) to 40 °C (104 °F) Chemical stability: All commonly used aqueous buffers, 8 M urea, 6 M guanidine hydrochloride and 20 % ethanol solution.
    [Show full text]
  • Method for Influenza Virus Purification Verfahren Zur Reinigung Von Influenzaviren Procédé De Purification De Virus De La Grippe
    (19) TZZ ¥__T (11) EP 2 361 975 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12N 7/02 (2006.01) 10.12.2014 Bulletin 2014/50 (21) Application number: 11164648.5 (22) Date of filing: 06.07.2007 (54) Method for influenza virus purification Verfahren zur Reinigung von Influenzaviren Procédé de purification de virus de la grippe (84) Designated Contracting States: (74) Representative: Von Kreisler Selting Werner - AT BE BG CH CY CZ DE DK EE ES FI FR GB GR Partnerschaft HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE von Patentanwälten und Rechtsanwälten mbB SI SK TR Deichmannhaus am Dom Bahnhofsvorplatz 1 (30) Priority: 11.07.2006 EP 06116979 50667 Köln (DE) 13.07.2006 US 830339 P (56) References cited: (43) Date of publication of application: EP-A- 0 171 086 31.08.2011 Bulletin 2011/35 • BRANOVIC K. ET AL.: "Application of short (62) Document number(s) of the earlier application(s) in monolithinc columns for improved detection of accordance with Art. 76 EPC: viruses", J. VIROL. METH., vol. 110, 2003, pages 07787173.9 / 2 041 273 163-171, XP002411116, • KRAMBERGER P, GLOVER D & STRANCAR A.: (73) Proprietors: "Application of monolithic columns for the fast • Bia Separations D.O.O. separation of nanoparticles", AMERICAN 5270 Ajdovscina (SI) BIOTECHNOLOGY LABORATORY, December • Baxter Healthcare SA 2003 (2003-12), pages 27-28, XP002411117, 8152 Glattpark (CH) • MERHAR MOJCA ET AL: "Methacrylate monoliths prepared from various hydrophobic (72) Inventors: and hydrophilic monomers: Structural and • Peterka, Matjaz chromatographic characteristics.", JOURNAL 1000 Ljubljana (SI) OF SEPARATION SCIENCE, vol.
    [Show full text]
  • Intensification of Influenza Virus Purification from Clarified Harvest to Formulated Product in a Single Shift
    Intensification of Influenza Virus Purification From Clarified Harvest to Formulated Product in a Single Shift M. Tajnik Sbaizero, M. Wolschek, M. Reiter, T. Muster, P. Gagnon, A. Štrancar nfluenza is a global respiratory disease with an estimated mortality of up to a half million I people per year (1). The majority of traditional influenza vaccines are still produced in eggs. Downstream processing typically consists of Vero cells infected with purified influenza A: Infectious virus particles were stained clarification by centrifugation, with rabbit antiinfluenza A NP-antibody and a goat antirabbit IgG antibody concentration by ultrafiltration, and conjugated with AlexaFluor 488 (green). Cell nuclei are stained blue with DAPI (4‘,6-diamidino-2-phenylindole). purification by ultracentrifugation (2). Recombinant vaccines are most often purified by chromatography. across the field of biopharmaceuticals. help companies focus resources on Chromatographic purification of One of those trends is process essential process functions. viruses already has achieved major intensification, referring to As with many viruses, purification of improvements in recovery and development of processes that influenza virus from clarified cell scalability (3), but it also is important harmonize integration of fewer and culture harvests often begins with because it enables virus purification more capable steps to achieve higher tangential-flow filtration (TFF), which to keep pace with important productivity and reproducibility as simultaneously achieves multiple tasks. regulatory and manufacturing trends well as reduce manufacturing costs. The first is concentration of the virus, Intensification takes many forms. which is retained, while contaminant Continuous processing represents one levels are reduced by passing through PRODUCT FOCUS: VACCINES aspect, and it already has been applied membrane pores.
    [Show full text]