Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 29 August 2018 doi:10.20944/preprints201808.0481.v1 1 Research Article 2 Comparative evaluation of polyclonal antibodies in 3 the characterization of nematocyst proteins from 4 Australian Irukandji and Chironex fleckeri species 5 Griselda Ávila-Soria1,2*, Lisa-Ann Gershwin 3,4, Kenneth D. Winkel 5, 6 and James N. Burnell2 6 7 8 1 Reef System Unit Institute of Marine Sciences and Limnology (ICML) of the National Autonomous 9 University of Mexico (UNAM), Puerto Morelos, Quintana Roo, México 10
[email protected] 11 12 2 Comparative Genomics Centre, Department of Biochemistry and Molecular Biology, School of Pharmacy 13 and Molecular Sciences, James Cook University, Townsville, Qld 4814;
[email protected] 14 15 3 16 17 4 CSIRO Oceans and Atmosphere, Castray Esplanade, Hobart, Tasmania, Australia; 18
[email protected] 19 20 5 Australian Marine Stinger Advisory Services, 5/20 Hampden Road, Battery Point, Tasmania, Australia; 21
[email protected] 22 23 6 Australian Venom Research Unit (AVRU), Department of Pharmacology, The University of Melbourne. 24 25 7 Indigenous Health Equity Unit, Centre for Health Equity, School of Population and Global Health, 26 Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne;
[email protected] 27 28 29 * Correspondence:
[email protected]; Tel.: +52-984-235-1034 30 31 Abstract: Carukia barnesi (Cb), Malo kingi (Mk) and Chironex fleckeri (Cf) are dangerous Australian 32 box jellyfish species that provoke distinct and not well understood envenomation syndromes. 33 Specifically, Cb and Mk are small, rare and able to induce a systemic syndrome of generalised 34 muscle pain and catecholamine excess termed “Irukandji syndrome”; Cf has been widely regarded 35 as one of the most venomous organisms in the animal kingdom causing severe sting site pain 36 combined with potentially lethal cardiotoxicity.