Girardia Dorotocephala Transcriptome Sequence, Assembly, and Validation Through Characterization of Piwi Homologs and Stem Cell Progeny Markers
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Diversity of Alien Macroinvertebrate Species in Serbian Waters
water Article Diversity of Alien Macroinvertebrate Species in Serbian Waters Katarina Zori´c* , Ana Atanackovi´c,Jelena Tomovi´c,Božica Vasiljevi´c,Bojana Tubi´c and Momir Paunovi´c Department for Hydroecology and Water Protection, Institute for Biological Research “Siniša Stankovi´c”—NationalInstitute of Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia; [email protected] (A.A.); [email protected] (J.T.); [email protected] (B.V.); [email protected] (B.T.); [email protected] (M.P.) * Correspondence: [email protected] Received: 29 September 2020; Accepted: 7 December 2020; Published: 15 December 2020 Abstract: This article provides the first comprehensive list of alien macroinvertebrate species registered and/or established in aquatic ecosystems in Serbia as a potential threat to native biodiversity. The list comprised field investigations, articles, grey literature, and unpublished data. Twenty-nine species of macroinvertebrates have been recorded since 1942, with a domination of the Ponto-Caspian faunistic elements. The majority of recorded species have broad distribution and are naturalized in the waters of Serbia, while occasional or single findings of seven taxa indicate that these species have failed to form populations. Presented results clearly show that the Danube is the main corridor for the introduction and spread of non-native species into Serbia. Keywords: Serbia; inland waters; allochthonous species; introduction 1. Introduction The Water Framework Directive (WFD) [1] represents key regulation and one of the most important documents in the European Union water legislation since it was adopted in 2000. -
Molecular Confirmation of the North American Leech Placobdella Ornata (Verrill, 1872) (Hirudinida: Glossiphoniidae) in Europe
BioInvasions Records (2015) Volume 4, Issue 3: 185–188 Open Access doi: http://dx.doi.org/10.3391/bir.2015.4.3.05 © 2015 The Author(s). Journal compilation © 2015 REABIC Rapid Communication Molecular confirmation of the North American leech Placobdella ornata (Verrill, 1872) (Hirudinida: Glossiphoniidae) in Europe Jan Soors1*, Joost Mertens2, William E. Moser3, Dennis J. Richardson4, Charlotte I. Hammond4 and Eric A. Lazo-Wasem5 1Research Institute for Nature and Forest, Kliniekstraat 25, 1070 Brussels, Belgium 2Vlaamse Milieumaatschappij (VMM), Raymonde de Larochelaan 1, 9051 Sint-Denijs-Westrem, Belgium 3Smithsonian Institution, National Museum of Natural History, Department of Invertebrate Zoology, Museum Support Center MRC 534, 4210 Silver Hill Road, Suitland, MD 20746 USA 4School of Biological Sciences, Quinnipiac University, 275 Mt. Carmel Avenue, Hamden, Connecticut 06518 USA 5Division of Invertebrate Zoology, Peabody Museum of Natural History, Yale University, P.O. Box 208118, New Haven, Connecticut 06520 USA E-mail: [email protected] (JS), [email protected] (JM), [email protected] (WEM), [email protected] (DJR), [email protected] (CIH), [email protected] (EALW) *Corresponding author Received: 28 January 2015 / Accepted: 15 May 2015 / Published online: 12 June 2015 Handling editor: Vadim Panov Abstract Specimens of the North American leech, Placobdella ornata (Verrill, 1872) were confirmed from the Donkmeer, a freshwater lake in the province of East Flanders, Belgium, by morphological and molecular analysis. Leech specimens from Belgium were morphologically consistent with the syntype series and description of P. ornata by Verrill (1872). Molecular comparison of the Belgian specimens to specimens of P. ornata from the type locality (New Haven, Connecticut, USA) using the cytochrome c oxidase subunit I (COI) gene revealed a similarity of 99.5%. -
Chromosome Numbers in Three Species Groups of Freshwater flatworms Increase with Increasing Latitude Sven Lorch, Dirk Zeuss, Roland Brandl & Martin Brandle€
Chromosome numbers in three species groups of freshwater flatworms increase with increasing latitude Sven Lorch, Dirk Zeuss, Roland Brandl & Martin Brandle€ Department of Ecology, Animal Ecology, Faculty of Biology, Philipps-Universitat€ Marburg, Karl-von-Frisch-Straße 8, 35043 Marburg, Germany Keywords Abstract Geographical range, parthenogenesis, Platyhelminthes, polyploidy, reproduction. Polyploidy in combination with parthenogenesis offers advantages for plasticity and the evolution of a broad ecological tolerance of species. Therefore, a posi- Correspondence tive correlation between the level of ploidy and increasing latitude as a surro- Martin Brandle,€ Department of Ecology, gate for environmental harshness has been suggested. Such a positive Animal Ecology, Faculty of Biology, Philipps- correlation is well documented for plants, but examples for animals are still € Universitat Marburg, Karl-von-Frisch-Straße 8, rare. Species of flatworms (Platyhelminthes) are widely distributed, show a 35043 Marburg, Germany. remarkably wide range of chromosome numbers, and offer therefore good Tel: +49 6421 28 26607; Fax: +49 6421 28 23387; model systems to study the geographical distribution of chromosome numbers. E-mail: [email protected] We analyzed published data on counts of chromosome numbers and geographi- cal information of three flatworm “species” (Phagocata vitta, Polycelis felina and Funding Information Crenobia alpina) sampled across Europe (220 populations). We used the mean DZ is supported by a PhD scholarship from chromosome number across individuals of a population as a proxy for the level Evangelisches Studienwerk Villigst, funded by of ploidy within populations, and we tested for relationships of this variable the German Federal Ministry of Education with latitude, mode of reproduction (sexual, asexual or both) and environmen- and Research tal variables (annual mean temperature, mean diurnal temperature range, mean Received: 2 March 2015; Revised: 16 precipitation and net primary production). -
R E S E a R C H / M a N a G E M E N T Aquatic and Terrestrial Flatworm (Platyhelminthes, Turbellaria) and Ribbon Worm (Nemertea)
RESEARCH/MANAGEMENT FINDINGSFINDINGS “Put a piece of raw meat into a small stream or spring and after a few hours you may find it covered with hundreds of black worms... When not attracted into the open by food, they live inconspicuously under stones and on vegetation.” – BUCHSBAUM, et al. 1987 Aquatic and Terrestrial Flatworm (Platyhelminthes, Turbellaria) and Ribbon Worm (Nemertea) Records from Wisconsin Dreux J. Watermolen D WATERMOLEN Bureau of Integrated Science Services INTRODUCTION The phylum Platyhelminthes encompasses three distinct Nemerteans resemble turbellarians and possess many groups of flatworms: the entirely parasitic tapeworms flatworm features1. About 900 (mostly marine) species (Cestoidea) and flukes (Trematoda) and the free-living and comprise this phylum, which is represented in North commensal turbellarians (Turbellaria). Aquatic turbellari- American freshwaters by three species of benthic, preda- ans occur commonly in freshwater habitats, often in tory worms measuring 10-40 mm in length (Kolasa 2001). exceedingly large numbers and rather high densities. Their These ribbon worms occur in both lakes and streams. ecology and systematics, however, have been less studied Although flatworms show up commonly in invertebrate than those of many other common aquatic invertebrates samples, few biologists have studied the Wisconsin fauna. (Kolasa 2001). Terrestrial turbellarians inhabit soil and Published records for turbellarians and ribbon worms in leaf litter and can be found resting under stones, logs, and the state remain limited, with most being recorded under refuse. Like their freshwater relatives, terrestrial species generic rubric such as “flatworms,” “planarians,” or “other suffer from a lack of scientific attention. worms.” Surprisingly few Wisconsin specimens can be Most texts divide turbellarians into microturbellarians found in museum collections and a specialist has yet to (those generally < 1 mm in length) and macroturbellari- examine those that are available. -
Method of Isolation and Characterization of Girardia Tigrina Stem Cells
BIOMEDICAL REPORTS 3: 163-166, 2015 Method of isolation and characterization of Girardia tigrina stem cells K.A.R. LOPES1,2, N.M.R. de CAMPOS VELHO2 and C. PACHECO-SOARES2 1Laboratory Planarians, Nature Study Center, University of Vale do Paraíba; 2Laboratory of Dynamics of Cellular Compartments, Institute of Research and Development, University of Vale do Paraíba, São José dos Campos, SP 12244-000, Brazil Received December 4, 2014; Accepted December 10, 2014 DOI: 10.3892/br.2014.408 Abstract. Tissue regeneration is widely studied due to its incubation and centrifugation. Antibody anti-OCT4 was used importance for understanding the biology of stem cells, aiming for the characterization of stem cells and was successfully at their application in medicine for therapeutic and various other labeled with concentrated neoblasts on interphase 1. purposes. The establishment of experimental models is neces- sary, as certain invertebrates and vertebrates have different Introduction regeneration abilities depending on their taxon position on the evolutionary scale. Planarians are an efficacious in vivo model Regeneration is a complex event that occurs in several verte- for stem cell biology, but the correlation between planarian brates and invertebrates (1). For regeneration to occur, one of cellular and molecular neoblast pluripotency mechanisms and the earliest signaling events following a lesion is the produc- those of mammalian stem cells is unknown. The present study tion of cells that are capable of rebuilding lost structures. The had the following objectives: i) Establish Girardia tigrina way that these events occur and the types of cells involved cell culture, ii) determine the time required for complete cell differ between animal groups (2). -
Neuropeptide Systems As Targets for Parasite and Pest Control ADVANCES in EXPERIMENTAL MEDICINE and BIOLOGY
Neuropeptide Systems as Targets for Parasite and Pest Control ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY Editorial Board: NATHAN BACK, State University of New York at Buffalo IRUN R. COHEN, The Weizmann Institute of Science ABEL LAJTHA, N.S. Kline Institute for Psychiatric Research JOHN D. LAMBRIS, University of Pennsylvania RODOLFO PAOLETTI, University of Milan Recent Volumes in this Series Volume 684 MEMORY T CELLS Edited by Maurizio Zanetti and Stephen P. Schoenberger Volume 685 DISEASES OF DNA REPAIR Edited by Shamim I. Ahmad Volume 686 RARE DISEASES EPIDEMIOLOGY Edited by Manuel Posada de la Paz and Stephen C. Groft Volume 687 BCL-2 PROTEIN FAMILY: ESSENTIAL REGULATORS OF CELL DEATH Edited by Claudio Hetz Volume 688 SPHINGOLIPIDS AS SIGNALING AND REGULATORY MOLECULES Edited by Charles Chalfant and Maurizio Del Poeta Volume 689 HOX GENES: STUDIES FROM THE 20TH TO THE 21ST CENTURY Edited by Jean S. Deutsch Volume 690 THE RENIN-ANGIOTENSIN SYSTEM: CURRENT RESEARCH PROGRESS IN THE PANCREAS Edited by Po Sing Leung Volume 691 ADVANCES IN TNF FAMILY RESEARCH Edited by David Wallach Volume 692 NEUROPEPTIDE SYSTEMS AS TARGETS FOR PARASITE AND PEST CONTROL Edited by Timothy G. Geary and Aaron G. Maule A Continuation Order Plan is available for this series. A continuation order will bring delivery of each new volume immediately upon publication. Volumes are billed only upon actual shipment. For further information please contact the publisher. Neuropeptide Systems as Targets for Parasite and Pest Control Edited by Timothy G. Geary, PhD Institute of Parasitology, McGill University, Montréal, Québec, Canada Aaron G. Maule, PhD Parasitology, School of Biological Sciences, Medical Biology Centre, Queen’s University Belfast, Belfast, UK Springer Science+Business Media, LLC Landes Bioscience Springer Science+Business Media, LLC Landes Bioscience Copyright ©2010 Landes Bioscience and Springer Science+Business Media, LLC All rights reserved. -
Freshwater Planarians (Platyhelminthes, Tricladida) from the Iberian Peninsula and Greece: Diversity and Notes on Ecology
Zootaxa 2779: 1–38 (2011) ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ Article ZOOTAXA Copyright © 2011 · Magnolia Press ISSN 1175-5334 (online edition) Freshwater planarians (Platyhelminthes, Tricladida) from the Iberian Peninsula and Greece: diversity and notes on ecology MIQUEL VILA-FARRÉ1,5, RONALD SLUYS2, ÍO ALMAGRO3, METTE HANDBERG-THORSAGER4 & RAFAEL ROMERO1 1Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain 2Institute for Biodiversity and Ecosystem Dynamics & Zoological Museum, University of Amsterdam, Ph. O. Box 94766, 1090 GT Amsterdam, The Netherlands 3Departamento de Biología Evolutiva y Biodiversidad. Museo Nacional de Ciencias Naturales, Madrid, Spain 4European Molecular Biology Laboratory, Developmental Biology Programme, Meyerhofstrasse 1, 69012 Heidelberg, Germany 5Corresponding author. E-mail: [email protected] Table of contents Abstract . 2 Introduction . 2 Material and methods . 4 Order Tricladida Lang, 1884 . 5 Suborder Continenticola Carranza, Littlewood, Clough, Ruiz-Trillo, Baguñà & Riutort, 1998 . 5 Family Dendrocoelidae Hallez, 1892 . 5 Genus Dendrocoelum Örsted, 1844 . 5 Dendrocoelum spatiosum Vila-Farré & Sluys, sp. nov. 5 Dendrocoelum inexspectatum Vila-Farré & Sluys, sp. nov. 10 Family Planariidae Stimpson, 1857 . 12 Genus Phagocata Leidy, 1847 . 12 Phagocata flamenca Vila-Farré & Sluys, sp. nov. 12 Phagocata asymmetrica Vila-Farré & Sluys, sp. nov. 15 Phagocata gallaeciae Vila-Farré & Sluys, sp. nov. 18 Phagocata pyrenaica Vila-Farré & Sluys, sp. nov. 20 Phagocata sp. 24 Phagocata hellenica Vila-Farré & Sluys, sp. nov. 24 Phagocata graeca Vila-Farré & Sluys, sp. nov. 27 Genus Polycelis Ehrenberg, 1831 . 30 Polycelis nigra (Müller, 1774) . 30 Family Dugesiidae Ball, 1974 . 30 Genus Girardia Ball, 1974 . 30 Girardia tigrina (Girard, 1850). 30 Genus Schmitdtea Ball, 1974. 31 Schmidtea polychroa (Schmidt, 1861) . -
Dugesia Japonica Is the Best Suited of Three Planarian Species for High-Throughput
bioRxiv preprint doi: https://doi.org/10.1101/2020.01.23.917047; this version posted January 24, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Dugesia japonica is the best suited of three planarian species for high-throughput 2 toxicology screening 3 Danielle Irelanda, Veronica Bocheneka, Daniel Chaikenb, Christina Rabelera, Sumi Onoeb, Ameet 4 Sonib, and Eva-Maria S. Collinsa,c* 5 6 a Department of Biology, Swarthmore College, Swarthmore, Pennsylvania, United States of 7 America 8 b Department of Computer Science, Swarthmore College, Swarthmore, Pennsylvania, United 9 States of America 10 c Department of Physics, University of California San Diego, La Jolla, California, United States of 11 America 12 13 14 15 16 * Corresponding author 17 Email: [email protected] (E-MSC) 18 Address: Martin Hall 202, 500 College Avenue, Swarthmore College, Swarthmore, PA 19081 19 Phone number: 610-690-5380 20 21 22 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.01.23.917047; this version posted January 24, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 23 Abstract 24 High-throughput screening (HTS) using new approach methods is revolutionizing 25 toxicology. Asexual freshwater planarians are a promising invertebrate model for neurotoxicity 26 HTS because their diverse behaviors can be used as quantitative readouts of neuronal function. -
Downloaded from the Planmine Database (31)
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.01.183442; this version posted July 2, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. A new species of planarian flatworm from Mexico: Girardia guanajuatiensis Elizabeth M. Duncan1†, Stephanie H. Nowotarski2,5†, Carlos Guerrero-Hernández2, Eric J. Ross2,5, Julia A. D’Orazio1, Clubes de Ciencia México Workshop for Developmental Biology3, Sean McKinney2, Longhua Guo4, Alejandro Sánchez Alvarado2,5* † Equal contributors. 1 University of Kentucky, Lexington KY, USA. 2 Stowers Institute for Medical Research, Kansas City MO, USA. 3 Clubes de Ciencia México, Guanajuato, GT, México. 4 University of California, Los Angeles CA, USA 5 Howard Hughes Medical Institute, Kansas City MO, USA. Keywords planarian, Girardia, Mexico, regeneration, stem cells ABSTRACT Background Planarian flatworms are best known for their impressive regenerative capacity, yet this trait varies across species. In addition, planarians have other features that share morphology and function with the tissues of many other animals, including an outer mucociliary epithelium that drives planarian locomotion and is very similar to the epithelial linings of the human lung and oviduct. Planarians occupy a broad range of ecological habitats and are known to be sensitive to changes in their environment. Yet, despite their potential to provide valuable insight to many different fields, very few planarian species have been developed as laboratory models for mechanism-based research. Results Here we describe a previously undocumented planarian species, Girardia guanajuatiensis (G.gua). -
Behavioral Parameters of Planarians (Girardia Tigrina) As Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results
water Article Behavioral Parameters of Planarians (Girardia tigrina) as Fast Screening, Integrative and Cumulative Biomarkers of Environmental Contamination: Preliminary Results Ana M. Córdova López 1,2 , Althiéris de Souza Saraiva 3, Carlos Gravato 4,* , Amadeu M. V. M. Soares 1,5 and Renato Almeida Sarmento 1 1 Programa de Pós-Graduação em Produção Vegetal, Campus Universitário de Gurupi, Universidade Federal do Tocantins, Gurupi-Tocantins 77402-970, Brazil; [email protected] 2 Estación Experimental Agraria Vista Florida, Dirección de Desarrollo Tecnológico Agrario, Instituto Nacional de Innovación Agraria (INIA), Carretera Chiclayo-Ferreñafe Km. 8, Chiclayo, Lambayeque 14300, Peru; [email protected] 3 Laboratório de Agroecossistemas e Ecotoxicologia, Instituto Federal de Educação, Ciência e Tecnologia Goiano-Campus Campos Belos, Campos Belos-Goiás 73840-000, Brazil; [email protected] 4 Faculdade de Ciências & CESAM, Universidade de Lisboa, 1749-016 Lisboa, Portugal 5 Departamento de Biologia & CESAM, Campus Universitário de Santiago, Universidade de Aveiro, 3810-193 Aveiro, Portugal; [email protected] * Correspondence: [email protected] Citation: López, A.M.C.; Saraiva, Abstract: The present study aims to use behavioral responses of the freshwater planarian Girardia A.d.S.; Gravato, C.; Soares, A.M.V.M.; tigrina to assess the impact of anthropogenic activities on the aquatic ecosystem of the watershed Sarmento, R.A. Behavioral Araguaia-Tocantins (Tocantins, Brazil). Behavioral responses are integrative and cumulative tools that Parameters of Planarians (Girardia reflect changes in energy allocation in organisms. Thus, feeding rate and locomotion velocity (pLMV) tigrina) as Fast Screening, Integrative were determined to assess the effects induced by the laboratory exposure of adult planarians to water and Cumulative Biomarkers of samples collected in the region of Tocantins-Araguaia, identifying the sampling points affected by Environmental Contamination: contaminants. -
Can Rice Field Channels Contribute to Biodiversity Conservation in Southern Brazilian Wetlands?
Can rice field channels contribute to biodiversity conservation in Southern Brazilian wetlands? Leonardo Maltchik1*, Ana Silvia Rolon1,2, Cristina Stenert1,2, Iberê Farina Machado1 & Odete Rocha2 1. Laboratory of Ecology and Conservation of Aquatic Ecosystems Av. Unisinos, 950 CEP 93.022-000, UNISINOS, São Leopoldo, RS, Brazil; [email protected], [email protected] 2. UFSCar, São Carlos, Brazil; [email protected], [email protected], [email protected] * Corresponding author Received 03-IX-2010. Corrected 10-II-2011. Accepted 15-III-2011. Abstract: Conservation of species in agroecosystems has attracted attention. Irrigation channels can improve habitats and offer conditions for freshwater species conservation. Two questions from biodiversity conservation point of view are: 1) Can the irrigated channels maintain a rich diversity of macrophytes, macroinvertebrates and amphibians over the cultivation cycle? 2) Do richness, abundance and composition of aquatic species change over the rice cultivation cycle? For this, a set of four rice field channels was randomly selected in Southern Brazilian wetlands. In each channel, six sample collection events were carried out over the rice cultivation cycle (June 2005 to June 2006). A total of 160 taxa were identified in irrigated channels, including 59 macrophyte species, 91 taxa of macroinvertebrate and 10 amphibian species. The richness and abundance of macrophytes, macroinvertebrates and amphibians did not change significantly over the rice cultivation cycle. However, the species composition of these groups in the irrigation channels varied between uncultivated and cultivated peri- ods. Our results showed that the species diversity found in the irrigation channels, together with the permanence of water enables these man-made aquatic networks to function as important systems that can contribute to the conservation of biodiversity in regions where the wetlands were converted into rice fields. -
Appendix 9 National-Scale Responses of Macroinvertebrates
Appendix 9: National-scale responses of river macroinvertebrates species to changes in temperature and precipitation Abstract Global climate change is expected to have a large impact on the biodiversity and functioning of freshwater ecosystems because of shifts in temperature, seasonality and weather. The response of freshwater organisms to climate change is likely to vary according to their environmental optima, with some species thriving under new conditions, while some at risk species may decline in abundance. These changes could significantly alter biodiversity, trophic interactions and key ecological processes, affecting current and future management and conservation regimes, as well as compliance with current environmental legislation such as the Water Framework Directive. This study examines the response of 137 river macroinvertebrate species to two climatic variables (temperature and precipitation) from 1,588 sampling sites across the United Kingdom over 15 to 25 years (1983-2007). Using a bespoke modelling method, the sensitivity of each species to changes in temperature and precipitation was identified, with the aim of inferring likely changes in the abundance of particular species in response to climate change. The characterisations of species responses are also used to demonstrate that a combination of species-specific traits and environmental preferences may be a systematic way to predict impacts. Introduction Freshwater ecosystems are considered one of the richest ecosystems globally in terms of biodiversity, sustaining a disproportionate high fraction of species per surface area relative to other ecosystems (Dudgeon et al., 2006, Balian et al., 2008). This biodiversity supports a range of important ecosystem processes (Woodward, 2009) , many of which provide key goods and services, such as the supply of clean drinking water, the dilution of pollution and the harvest of fish and other produce, to name but a few (Millennium Ecosystem Assessment, 2005).