bioRxiv preprint doi: https://doi.org/10.1101/073650; this version posted September 6, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Investigation of the sexual reproduction strategy of the Périgord black truffle (Tuber 2 melanosporum Vittad.) revealed trioecy 3 4 Herminia De la Varga1, François Le Tacon1, Mélanie Lagoguet1, Flora Todesco1, Torda 5 Varga1*, Igor Miquel1, Dominique Barry-Etienne2, Christophe Robin3, Fabien Halkett1, 6 Francis Martin1, Claude Murat1,4 7 8 1INRA, Université de Lorraine, UMR1136 Interactions Arbres-Microorganismes, Laboratoire 9 d’Excellence ARBRE, F-54280 Champenoux, France 10 2ALCINA sarl, 10 rue des Amaryllis, F-34070 Montpellier, France 11 3 INRA, Université de Lorraine, UMR 1121 Laboratoire Agronomie-Environnement, Nancy- 12 Colmar, Vandoeuvre-les-Nancy 54518, France 13 14 4Corresponding author: 15 Claude Murat 16 UMR1136 INRA/Université de Lorraine. “Interactions Arbres/Micro-organismes” 17 Laboratoire d’Excellence ARBRE. INRA Nancy. 54280 Champenoux. France 18 Tel: +33 (0)3 83 39 41 27; Fax: +33 (0)3 83 39 40 69; Email:
[email protected] 19 20 Running title: Black truffle sexual reproduction strategy * Current address: Synthetic and Systems Biology Unit, Biological Research Centre, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. 1 bioRxiv preprint doi: https://doi.org/10.1101/073650; this version posted September 6, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 21 Summary 22 Fungi display a rich variety of sexual reproduction systems and are therefore good models to 23 investigate sex evolution.