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Investigation of the Underlying Hub Genes and Molexular Pathogensis in Gastric Cancer by Integrated Bioinformatic Analyses
bioRxiv preprint doi: https://doi.org/10.1101/2020.12.20.423656; this version posted December 22, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Investigation of the underlying hub genes and molexular pathogensis in gastric cancer by integrated bioinformatic analyses Basavaraj Vastrad1, Chanabasayya Vastrad*2 1. Department of Biochemistry, Basaveshwar College of Pharmacy, Gadag, Karnataka 582103, India. 2. Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karanataka, India. * Chanabasayya Vastrad [email protected] Ph: +919480073398 Chanabasava Nilaya, Bharthinagar, Dharwad 580001 , Karanataka, India bioRxiv preprint doi: https://doi.org/10.1101/2020.12.20.423656; this version posted December 22, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract The high mortality rate of gastric cancer (GC) is in part due to the absence of initial disclosure of its biomarkers. The recognition of important genes associated in GC is therefore recommended to advance clinical prognosis, diagnosis and and treatment outcomes. The current investigation used the microarray dataset GSE113255 RNA seq data from the Gene Expression Omnibus database to diagnose differentially expressed genes (DEGs). Pathway and gene ontology enrichment analyses were performed, and a proteinprotein interaction network, modules, target genes - miRNA regulatory network and target genes - TF regulatory network were constructed and analyzed. Finally, validation of hub genes was performed. The 1008 DEGs identified consisted of 505 up regulated genes and 503 down regulated genes. -
Disease Factsheet: Warble
Livestock Notifiable Disease Factsheets Warble Fly If you suspect signs of any notifiable disease, you must immediately notify a Defra Divisional Veterinary Manager. Introduction The warble fly is an insect, which parasitically infects cattle. Animals usually affected are Cattle although horses and deer can be affected. History and spread of the disease Around thirty years ago the industry was concerned that warble fly infestation was not only causing distress to cattle and financial losses to farmers, but was also ruining hides that would otherwise be used to make items such as coats, bags, and shoes. There were believed to be around 4 million cases of warble fly every year at that time. On 7 February 1978 the MAFF Parliamentary Secretary announced a five-year campaign to eradicate the warble fly. This included an extensive publicity campaign in summer and autumn each year to encourage all farmers with cattle at risk from the fly to treat them as a precaution in the autumn and also to treat any cattle showing visible signs of infestation. Legislation made under the Diseases of Animals Act 1950 required owners of warbled cattle to treat them with a systemic dressing: affected animals could not be moved, even to a slaughterhouse, without first being treated. Veterinary inspectors could compel owners to treat affected cattle. The farming community responded splendidly to this campaign involving both voluntary and compulsory treatment, with the help of market surveys by the State Veterinary Service, the Meat & Livestock Commission and the Hide & Allied Trade Improvement Society. At the end of the five year period the number of outbreaks was down to around 500 a year. -
Inflammatory Reaction to the Human Bot-Fly, Dermatobia Hominis, in Infested and Reinfested Mice
Medical and Veterinary Entomology (2003) 17, 55±60 Inflammatory reaction to the human bot-fly, Dermatobia hominis, in infested and reinfested mice * E. LELLO and A. M. B. DE ROSIS Departamento de Morfologia, Instituto de Biocieà ncias, Universidade Estadual Paulista, Botucatu and *Departamento de Cieà ncias Biolo gicas, Faculdade de Cieà ncias, Universidade Estadual Paulista, Bauru, SP, Brazil Abstract. Two groups of mice were infested with first stage larvae of the human bot-fly, Dermatobia hominis (Linnaeus Jr) (Diptera: Oestridae). In the first group, skin biopsies were carried out 1, 3, 5, 7, 10 and 18 days after infestation. The second group was also infested but had all the larvae removed 5 days after infestation. The mice in the latter group were reinfested 4 weeks later and skin biopsies were carried out 1, 3, 5, 7, 10 and 18 days after reinfestation. In the first group, an inflammatory reaction began slowly, the neutrophils being the main inflammatory cells, eosinophils being scarce. The reaction progressed with time, developing a necrotic halo around the larvae containing inflammatory cells sur- rounded by fibroblasts. The inflammation invaded the adjacent tissue. In the second group, the inflammatory reaction was intense on the day immediately after reinfestation, the pattern being changed by the presence of a large number of eosinophils. Activated fibroblasts surrounding the necrotic area around the larvae appeared 3 days after reinfestation in the second group and 7 days after infestation in the first group. The results demonstrated that the previous contact with the antigens elicited the early arrival of eosinophils, probably through the chemotactic factors liberated by mast cells in the anaphylactic reaction. -
The Ox Warble Flies
BULLETIN 428 NOVEMBER, 1928 The Ox Warble Flies Hypoderma bovis de Geer Hypoderma lineatum de Villers Don C. Mote OHIO AGRICULTURAL EXPERIMENT STATION \Vooster, Ohio CONTENTS Introduction . .. 3 Hosts .............................................................. 4 Effect of the Warble Flies on Man and Animals . .. 5 Economic Importance . .. 8 Distribution . ................................ 10 Biology and Habits . 12 Larvae in the Gullet and Spinal Canal . 19 Emergence of Adults ................................................. 27 Combatting the Warble Flies ......................................... 28 Extracting and Destroying . 28 Larvacides . ............. 31 Fly Repellents, Ovicides . 34 Medicinal Treatment . 35 Natural Immunity ................................................ 35 Protection of Cattle by Housing ................................... 36 H. bovis and H. linea tum Differentiated ................................ 36 The Adult . 37 The Egg ........................................................ 38 The Larvae . 38 The Puparia . 42 Literature Cited .................................................. , . 43 (1) This page intentionally blank. THE OX WARBLE FLIES~· Hypoderma bovis de Geer Hypoderma lineatum de Villers Order, Diptera Family, Oestridae DON C. MOTE INTRODUCTION Domestic cattle in Ohio are subject to attack by two species of warble flies-Hypoderma bovis de Geer and Hypoderma lineatum de Villers. Every cattleman is familiar with grubs that produce the tumors or warbles on the backs of cattle in the spring and nearly -
Reindeer Warble Fly Larvae Found in Red Deer Nilssen, A. C.1
Reindeer warble fly larvae found in red deer Reinens gorm-larver funnet i hjort Nilssen, A. C.1 & Gjershaug, J. O.2. 1. Zoology Department, Tromsø Museum, N-9000 Tromsø, Norway. 2. Sognli Forsøksgård, N-7320 Fannrem, Norway. Abstract: Seven third instar larvae of the reindeer warble fly (Hypoderma (=Oedemagena) tarandi) were found in a 2-3 year old male red deer {Cervus elaphus) shot on 14 November 1985 at Todalen, western Norway. This it, the first report of H. tarandi from red deer. In reindeer third instar larvae are found from February to June, and the unusual date of this record indicates a delayed development of the larvae due to abnormal host reactions. Warble fly larvae, probably H. tarandi, are also reported from moose {Alces al• ces) in northern Norway. Key words: Oedemagena tarandi, Hypoderma tarandi, Cervus elaphus, Rangifer tarandus, Alces alces, reindeer, caribou, red deer, moose, exotic host, unsuitable host, reindeer warble fly, Norway. Rangifer, 8 (1): 35-37 Introduction and first and second instar larvae also from The warble fly Hypoderma tarandi is endopa- musk ox {Ovibos moschatus) in Canada (Jan- rasitic in reindeer and caribou {Rangifer ta• sen 1970, Alendal and Helle 1983). Nordkvist randus), occurring in most of the holarctic (in Skjenneberg and Slagsvold 1968) reported distribution of the host (Zumpt 1965). The H. tarandi from roe deer {Capreolus capreo• adult females attach eggs to leg and body lus), and this appears to be the only record of hairs. After hatching the larvae penetrate the H. tarandi in a cervid other than reindeer. skin, migrate intermuscularly and complete Here we report H. -
Parasites of Caribou (2): Fly Larvae Infestations
Publication AP010 July 27, 2004 Wildlife Diseases FACTSHEET Parasites of Caribou (2): Fly Larvae Infestations Introduction All wild animals carry diseases. In some cases these might be of concern if they can spread to humans or domestic animals. In other cases they might be of interest if they impact on the health of our wild herds, or simply because the signs of the disease have been noticed and you want to know more. This factsheet is one of a series produced on the common diseases of caribou and covers the larval form of two different flies commonly seen in this province. Neither of these are a cause of public health Figure 2: Warble fly larvae emerging from back concern. Fly Larvae Infestations Warbles are the larvae of the warble fly (Hypoderma tarandi) which lays its eggs on the hair of Warbles and throat bots are common names for the caribou’s legs and lower body in mid-summer. the larvae of two types of flies that can be found in the Once the eggs hatch, the larvae (maggots) penetrate carcasses of caribou in this province. Many people the skin of the caribou and start a long migration who hunt these animals may be familiar with their towards the animal’s back. appearance. Hunters may see signs of these migrating larvae Warbles in the fall when skinning out an animal. At this early part of the larvae’s life, they are about the size of a small grain of rice and are almost transparent. They may be seen on the surface of the muscles or under the skin. -
Myiasis in Humans and Animals
Animal Husbandry, Dairy and Veterinary Science Pilot Study ISSN: 2513-9304 Myiasis in humans and animals Vahedi Nouri N1*and Salehi A2 1Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran 2Department of Veterinary Medicine, Babol Islamic Azad University, Iran Abstract Myiasis is the contamination of live or dead tissue of vertebrates via the larvae of various flies. Myiasis occurred both in humans and animals. The myiasis contamination has a worldwide distribution. Different genera of three families which include: Oestridae, calliphoridae and Sarcophagidae have a role in creating myiasis in animals and humans. Myiasis in addition to health problems, can cause a lot of economic problems. Decrease of fly population as a causative of myiasis is one of the important ways to control this contamination. Also, surgical approaches and removing larvae of flies in infected parts, is the other treatment for the contamination. Introduction decaying organic matter and carcasses of animals. The external parasite producing secondary myiasis lives naturally in the form of a detritivore Although Kirby and Spence (1918) for the first time, used the term and usually cannot produce Myiasis but may attack previous living Scholechiasis for infected animals with fly larvae [1], Hoop (1840) used organism secondary [8]. the term Myiasis for many years before that [2]. Myiasis is a Greek word (Myia=Fly) meaning fly, which means the contamination of organs, Accidental myiasis living tissues or dead vertebrates (humans and all kinds of animals) In this type, the larvae of the fly create a random Myiasis and may with fly larvae. -
Regulation and Physiological Roles of the Calpain System in Muscular Disorders
Cardiovascular Research (2012) 96,11–22 SPOTLIGHT REVIEW doi:10.1093/cvr/cvs157 Regulation and physiological roles of the calpain system in muscular disorders Hiroyuki Sorimachi* and Yasuko Ono* Calpain Project, Department of Advanced Science for Biomolecules, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan Received 1 February 2012; revised 16 April 2012; accepted 24 April 2012; online publish-ahead-of-print 27 April 2012 + Abstract Calpains, a family of Ca2 -dependent cytosolic cysteine proteases, can modulate their substrates’ structure and func- tion through limited proteolytic activity. In the human genome, there are 15 calpain genes. The most-studied calpains, referred to as conventional calpains, are ubiquitous. While genetic studies in mice have improved our understanding about the conventional calpains’ physiological functions, especially those essential for mammalian life as in embryo- genesis, many reports have pointed to overactivated conventional calpains as an exacerbating factor in pathophysio- logical conditions such as cardiovascular diseases and muscular dystrophies. For treatment of these diseases, calpain inhibitors have always been considered as drug targets. Recent studies have introduced another aspect of calpains that calpain activity is required to protect the heart and skeletal muscle against stress. This review summarizes the functions and regulation of calpains, focusing on the relevance of calpains to cardiovascular disease. ----------------------------------------------------------------------------------------------------------------------------------------------------------- -
Calpain 9 (CAPN9) Mouse Monoclonal Antibody [Clone ID: OTI1C2] Product Data
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA503569 Calpain 9 (CAPN9) Mouse Monoclonal Antibody [Clone ID: OTI1C2] Product data: Product Type: Primary Antibodies Clone Name: OTI1C2 Applications: IF, WB Recommended Dilution: WB 1:2000, IF 1:100 Reactivity: Human, Mouse, Rat Host: Mouse Isotype: IgG2a Clonality: Monoclonal Immunogen: Full length human recombinant protein of human CAPN9(NP_006606) prodced in HEK293T cell. Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Concentration: 0.9 mg/ml Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Predicted Protein Size: 78.9 kDa Gene Name: calpain 9 Database Link: NP_006606 Entrez Gene 116694 RatEntrez Gene 10753 Human O14815 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 Calpain 9 (CAPN9) Mouse Monoclonal Antibody [Clone ID: OTI1C2] – TA503569 Background: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. -
By Hypoderma Tarandi, Northern
INTERNATIONAL POLAR YEAR DISPATCHES by Hypoderma spp. is not known. Eyebrows and eyelashes Human have been suggested as possible targets for oviposition (3). Oviposition on human scalp hair has been achieved experi- Ophthalmomyiasis mentally and could be the preferential site in humans (3). An alternative explanation is transfer of the larvae directly Interna Caused from the guard hairs of the caribou to the human eye or skin through close contact with animal pelts. The parasite does by Hypoderma not appear to complete its life cycle in humans (1,2). tarandi, Northern We present the fi rst, to our knowledge, 2 published cases of ophthalmomyiasis interna caused by H. tarandi in Canada Canada. Furthermore, we present the fi rst published use of Hypoderma spp. serologic testing to assist in the diagnosis Philippe R.S. Lagacé-Wiens,* Ravi Dookeran,* of myiasis in humans. Stuart Skinner,* Richard Leicht,* Douglas D. Colwell,† and Terry D. Galloway* The Cases and Literature Review Human myiasis caused by bot fl ies of nonhuman ani- The fi rst patient was a 41-year-old woman from Rankin mals is rare but may be increasing. The treatment of choice Inlet, Nunavut, Canada, who noticed fl oaters (objects in the is laser photocoagulation or vitrectomy with larva removal fi eld of vision that originate in the vitreous) in her right eye and intraocular steroids. Ophthalmomyiasis caused by Hy- in August 2006. Initial funduscopic examination showed poderma spp. should be recognized as a potentially revers- posterior vitreous detachment. Two weeks later, her vision ible cause of vision loss. was more impaired; repeat funduscopy showed panuveitis. -
F. Christian Thompson Neal L. Evenhuis and Curtis W. Sabrosky Bibliography of the Family-Group Names of Diptera
F. Christian Thompson Neal L. Evenhuis and Curtis W. Sabrosky Bibliography of the Family-Group Names of Diptera Bibliography Thompson, F. C, Evenhuis, N. L. & Sabrosky, C. W. The following bibliography gives full references to 2,982 works cited in the catalog as well as additional ones cited within the bibliography. A concerted effort was made to examine as many of the cited references as possible in order to ensure accurate citation of authorship, date, title, and pagination. References are listed alphabetically by author and chronologically for multiple articles with the same authorship. In cases where more than one article was published by an author(s) in a particular year, a suffix letter follows the year (letters are listed alphabetically according to publication chronology). Authors' names: Names of authors are cited in the bibliography the same as they are in the text for proper association of literature citations with entries in the catalog. Because of the differing treatments of names, especially those containing articles such as "de," "del," "van," "Le," etc., these names are cross-indexed in the bibliography under the various ways in which they may be treated elsewhere. For Russian and other names in Cyrillic and other non-Latin character sets, we follow the spelling used by the authors themselves. Dates of publication: Dating of these works was obtained through various methods in order to obtain as accurate a date of publication as possible for purposes of priority in nomenclature. Dates found in the original works or by outside evidence are placed in brackets after the literature citation. -
Calpain-2 Participates in the Process of Calpain-1 Inactivation
Bioscience Reports (2020) 40 BSR20200552 https://doi.org/10.1042/BSR20200552 Research Article Calpain-2 participates in the process of calpain-1 inactivation Fumiko Shinkai-Ouchi1, Mayumi Shindo2, Naoko Doi1, Shoji Hata1 and Yasuko Ono1 1Calpain Project, Department of Basic Medical Sciences, Tokyo Metropolitan Institute of Medical Science (TMiMS), 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156- 8506, Japan; 2Center for Basic Technology Research, Tokyo Metropolitan Institute of Medical Science (TMiMS), 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156- 8506, Japan Downloaded from http://portlandpress.com/bioscirep/article-pdf/40/11/BSR20200552/896871/bsr-2020-0552.pdf by guest on 28 September 2021 Correspondence: Yasuko Ono ([email protected]) Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or complementary manner, as they are both ubiq- uitously expressed and activated in a Ca2+- dependent manner. However, studies using isoform-specific knockout and knockdown strategies revealed that each calpain species carries out specific functions in vivo. To understand the mechanisms that differentiate calpain-1 and calpain-2, we focused on the efficiency and longevity of each calpain species after activation. Using an in vitro proteolysis assay of troponin T in combination with mass spectrometry, we revealed distinctive aspects of each isoform. Proteolysis mediated by calpain-1 was more sustained, lasting as long as several hours, whereas proteolysis me- diated by calpain-2 was quickly blunted.