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Clonal Propagation of Phalaenopsis a Dissertation
CLONAL PROPAGATION OF PHALAENOPSIS A DISSERTATION SUBMITTED TO THE GRADUATE DIVISION OF THE UNIVERSITY OF HAWAII IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN HORTICULTURE MAY 1974 By Oradee Intuwong DISSERTATION COMMITTEE: Yoneo Sagawa, Chairman Haruyuki Kamemoto Charles H. Lamoureux Henry Y. Nakasone John T. Kunisaki Marion 0. Mapes We certify that we have read this dissertation and that in our opinion it is satisfactory in scope and quality as a dissertation for the degree of Doctor of Philosophy in Horticulture. DISSERTATION COMMITTEE CfaU6 Chairman 01j- <XAs<^<rv^ & ■ . ACKNOWLEDGEMENT The author expresses deep appreciation to the East-West Center, Harold L. Lyon Arboretum, Hawaii Agricultural Experiment Station, and Asia Foundation for their financial assistance to pursue graduate work at the University of Hawaii, and to Kodama Nursery for donation of some plant materials. ABSTRACT Phalaenopsis was clonally propagated by use of in vivo and _in vitro methods. In vivo, plantlets formed naturally on the node and tip of inflorescence, or root. Application of N-6-benzyl adenine to exposed buds on the inflorescence spike to induce plantlet formation was not very successful. Rapid clonal propagation was successfully accomplished by use of in vitro culture techniques. Explants from the nodal buds of inflorescence were the most suitable material for culture, although apical and axillary buds from the stem could also be used. When basal nodes from inflorescences after flowering or young inflorescences were cultured in basal media (BM = Vacin and Went 4- 15% coconut water), one to four plantlets rather than protocorm-like bodies (plbs) were obtained from a single node. -
Tools to Develop Genetic Model Plants in the Orchidaceae Family
ogy iol : Op r B e a n l A u c c c e l e Tsai and Sawa, Mol Biol 2018, 7:3 o s s M Molecular Biology: Open Access DOI: 10.4172/2168-9547.1000217 ISSN: 2168-9547 Short communication Open Access Tools to Develop Genetic Model Plants in the Orchidaceae Family Allen Yi-Lun Tsai and Shinichiro Sawa* Graduate School of Science & Technology, Kumamoto University, Kurokami 2-39-1, Kumamoto , Japan promote fungal growth contained within a plastic box [15]. Interestingly, Introduction G. pubilabiata was not only viable in the ACS, but was able to set seeds up to three times a year, compare to in the natural habitat where it may take at The Orchidaceae family is estimated to contain about 28,000 species least one year to set seed [15]. These results suggest that orchid generation and over 100,000 hybrids, making it one of the largest taxonomic time can potentially be shortened under artificial conditions, such that the groups among flowering plants [1]. The variations in flower colours, timeframes of genetic analyses become feasible. floral organ morphology and scents make orchids highly sought-after in ornamental horticulture. In addition, orchids are found in nearly In summary, advancements in the Orchidaceae family research all regions of the world with diverse adaptations, making them an highlight the orchids’ potential to be used as a genetic tool for basic invaluable resource to study plant evolution and speciation. research. One direction is to utilize orchid transposon as a mutation mapping tool. Phenotypic instability is a significant problem in the Despite orchids’ great economic values and potential in basic orchid breeding industry. -
Orchids Limited Catalog
2003-2004 Orchids Limited Catalog Hic Natus Ubique Notus (Born here, known everywhere!) 25 years ago, in 1978, Orchids Limited started in a very small greenhouse behind a plant store in Minnetonka, Minnesota. Now located in Plymouth, we have grown to five greenhouses, a lab and support building. Our goal has always been to provide high quality species and hybrids in a niche market. We continue to breed new varieties and raise large populations of species derived from select stock. We are now seeing the fruits of our labor with many new exciting hybrids. It is very satisfying to see populations of species that were once hard to obtain or produce, now become available through our laboratory. Thanks to all of our customers, past and present, who have supported us and enabled us to grow. Please visit our web site at www.orchidweb.com for the most up-to-date offerings. We have designed a custom search engine to allow you to search for plants by name, category, color, temperature, bloom season or price range. Or, simply click "Browse our entire selection" for an alphabetic listing of all items. Our In Spike Now section, updated weekly, lists all the plants in flower or bud that are ready to be shipped. The Plant of the Week feature and Plant of the Week Library provide pictures and detailed cultural information on numerous species and hybrids. Thank you for choosing Orchids Limited. Orchids Limited 4630 Fernbrook Lane N. Plymouth, MN 55446 U.S.A. www.orchidweb.com Toll free: 1-800-669-6006 Phone: 763-559-6425 Fax: 763-557-6956 e-mail: [email protected] Nursery Hours: Mon-Sat 9:00 a.m. -
Laelia5 Revista Laelia 2
Laelia REVISTA DEL GRUPO DE ESTUDIO Y CONSERVACIÓN DE ORQUÍDEAS Número 5 JULIO-AGOSTO 2009 www.gecor.org GECOR N º 5 07-08/2009 ES NOTICIA... JUNTA DIRECTIVA PROYECTO ORQUIDEA EN PÁ- sean producidas en territorio Grupo de Estudio y Conservación de orquídeas NAMA Y TAIWAN panameño. Presidente: Jose Ramón Pinela [email protected] Kevin Chen, jefe de la Misión Está propuesta es secundada Vicepresidente: Maria Jesús Arias técnica taiwanesa en Pánama por un invernadero de orquí- [email protected] afirmó recientemente que Pa- deas que ha sido creado en las Tesorería: Ana Sánchez namá junto con Taiwan colabo- instalaciones de la Universidad [email protected] ran en un proyecto para Tecnológica de Panamá con el Secretaría: Manuel Lucas [email protected] rescatar y comercializar algu- respaldo técnico de taiwan. Vocales: nos tipos de orquídeas. Diego Martínez En el Valle de Antón ubicado en [email protected] Este estudio surgió en el año el cráter de un volcán a 126 Rubén Velázquez 2008 en el distrito de Capira, km al oeste de la capital pana- [email protected] que está localizado a unos 54 meña, es un lugar dominado Emilio Esteban-Infantes km al oeste de la capital pana- por la jardinería. Existen nú- [email protected] meña, sus condiciones climáti- meros viveros en cualquier rin- cas para la agricultura son cón, rosas, orquídeas … aquí es Socios de honor Dª Gemma López Vélez semejantes a las zonas desti- donde se han seleccionado dos Dª Angela Mirro nadas a la floricultura de Tai- grupos para que reciban mate- wan. rial del laboratorio biotecnoló- gico para que se inicie el Esté proyecto científico incluye cultivo de forma organizada. -
A Note on Masdevallia Zahlbruckneri and M
A NOTE ON Masdevallia ZAHLBRUCKNERI AND M. utriculata (ORCHIDACEAE) LIZBETH OSES SALAS1, 2 AND ADAM P. KARREMANS1, 3 Abstract. Masdevallia utriculata and M. zahlbruckeri are characterized and illustrated based on living plants from Costa Rica. The species are compared with each other on the basis of their general similar morphology. Their taxonomic status is reviewed. Keywords: Masdevallia utriculata, M. zahlbruckneri, new record, taxonomy, Zahleria Heinrich Gustav Reichenbach was the first to propose Rchb.f. (Reichenbach f., 1874), which in turn Veitch and subdivisions of the genus Masdevallia Ruiz & Pav.; he Sons (1889) reduced to subsection. Luer (1986) retained published 14 sections in total (Reichenbach f., 1873, Masdevallia sect. Coriaceae and split into subsections 1874a, 1874b, 1875, 1876a, 1876b, 1877, 1878a, 1878b; Coriaceae (Rchb.f.) H.J.Veitch and Durae Luer. In 2000, Woolward, 1896; Luer, 1986), including Masdevallia sect. Luer elevated subsection Durae to the sectional rank, and Amandae Rchb.f. (Reichenbach f., 1874b). Luer (1986) in 2006 he reduced sect. Durae to the synonymy of his raised Amanda to the rank of subgenus and included five new genus Regalia (Luer, 2000, 2006). According to Luer, sections within it: Masdevallia sections Amandae, Fissae Regalia is a small Andean genus composed of ten species, Rchb.f, Nidificae Luer, Ophioglossae Luer and Pygmaeae characterized by large plants with stout ramicauls, thickly Luer. Luer would later elevate these sections to the rank of coriaceous leaves, and racemes of large, rigid, fleshy, long- subgenus (Luer, 2000). lasting, long-caudate flowers produced in slow succession. When establishing subgenus Pygmaeae Luer, the author The petals are thickly cartilaginous, paddle-shaped and united subgenus Masdevallia sections Amaluzae and truncate, and the lip is oblong and thick, with a pair of Aphanes with subgenus Amanda section Pygmaeae (Luer, concavities at the base (Luer, 2006). -
Carlyle A. Luer1 and Alv Toscano De
MiScellANeOuS NeW SpecieS iN The pleuROThAlliDiNAe (ORchiDAceAe) CARLYLE A. LUER1 AND A. L. V. TOSCANO DE BRITO2,3 Abstract. Two new species of Crocodeilanthe, C. dewildei and C. steinbachii, three new species of Masdevallia, M. calochrysos, M. driesseniana and M. rostriflora, and one new species of Pleurothallis, P. amentacea, are described and illustrated. Keywords: Crocodeilanthe, Masdevallia, Pleurothallis, Stelis Two species of Crocodeilanthe Rchb. f. & Warsz., This large species is characterized by a thick rhizome, three species of Masdevallia Ruiz & Pav., one species long ramicauls, and one or two many-flowered racemes of Pleurothallis R. Br. and one species of Stelis Sw. are shorter than an elliptical leaf; small, fleshy, obtuse sepals; described as new. oblong, membranous, single-veined petals; and a fleshy lip As discussed elsewhere (Toscano de Brito, 2018), the with erect, broadly rounded margins overlaid with similarly genus Crocodeilanthe is related to Stelis Sw. Vegetatively, shaped calli nearly to the tip. Crocodeilanthe is inseparable from many species of Stelis, Plant large, epiphytic, densely caespitose from a rhizome which is reflected in portions of their DNA. Species of 5 mm thick; roots slender. Ramicauls erect, stout, 18–23 cm Crocodeilanthe are distinguished by morphology of the long, with a close, tubular sheath on the middle third and sepals, petals, lip and column. The sepals of Crocodeilanthe 2–3 other sheaths below at the base. Leaf erect, coriaceous, are developed into a recognizable dorsal sepal, which is free elliptical, subacute, 14–16 cm long including a petiole 1–1.5 of variably connate to the lateral sepals that are also more mm long, the blade 3 cm wide in the dry state, cuneate below or less connate and antrorse. -
Systematics and Evolution of the Genus Pleurothallis R. Br
Systematics and evolution of the genus Pleurothallis R. Br. (Orchidaceae) in the Greater Antilles DISSERTATION zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) im Fach Biologie eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät I der Humboldt-Universität zu Berlin von Diplom-Biologe Hagen Stenzel geb. 05.10.1967 in Berlin Präsident der Humboldt-Universität zu Berlin Prof. Dr. J. Mlynek Dekan der Mathematisch-Naturwissenschaftlichen Fakultät I Prof. Dr. M. Linscheid Gutachter/in: 1. Prof. Dr. E. Köhler 2. HD Dr. H. Dietrich 3. Prof. Dr. J. Ackerman Tag der mündlichen Prüfung: 06.02.2004 Pleurothallis obliquipetala Acuña & Schweinf. Für Jakob und Julius, die nichts unversucht ließen, um das Zustandekommen dieser Arbeit zu verhindern. Zusammenfassung Die antillanische Flora ist eine der artenreichsten der Erde. Trotz jahrhundertelanger floristischer Forschung zeigen jüngere Studien, daß der Archipel noch immer weiße Flecken beherbergt. Das trifft besonders auf die Familie der Orchideen zu, deren letzte Bearbeitung für Cuba z.B. mehr als ein halbes Jahrhundert zurückliegt. Die vorliegende Arbeit basiert auf der lang ausstehenden Revision der Orchideengattung Pleurothallis R. Br. für die Flora de Cuba. Mittels weiterer morphologischer, palynologischer, molekulargenetischer, phytogeographischer und ökologischer Untersuchungen auch eines Florenteils der anderen Großen Antillen wird die Genese der antillanischen Pleurothallis-Flora rekonstruiert. Der Archipel umfaßt mehr als 70 Arten dieser Gattung, wobei die Zahlen auf den einzelnen Inseln sehr verschieden sind: Cuba besitzt 39, Jamaica 23, Hispaniola 40 und Puerto Rico 11 Spezies. Das Zentrum der Diversität liegt im montanen Dreieck Ost-Cuba – Jamaica – Hispaniola, einer Region, die 95 % der antillanischen Arten beherbergt, wovon 75% endemisch auf einer der Inseln sind. -
Partial Endoreplication Stimulates Diversification in the Species-Richest Lineage Of
bioRxiv preprint doi: https://doi.org/10.1101/2020.05.12.091074; this version posted May 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Partial endoreplication stimulates diversification in the species-richest lineage of 2 orchids 1,2,6 1,3,6 1,4,5,6 1,6 3 Zuzana Chumová , Eliška Záveská , Jan Ponert , Philipp-André Schmidt , Pavel *,1,6 4 Trávníček 5 6 1Czech Academy of Sciences, Institute of Botany, Zámek 1, Průhonice CZ-25243, Czech Republic 7 2Department of Botany, Faculty of Science, Charles University, Benátská 2, Prague CZ-12801, Czech Republic 8 3Department of Botany, University of Innsbruck, Sternwartestraße 15, 6020 Innsbruck, Austria 9 4Prague Botanical Garden, Trojská 800/196, Prague CZ-17100, Czech Republic 10 5Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, Prague CZ- 11 12844, Czech Republic 12 13 6equal contributions 14 *corresponding author: [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.05.12.091074; this version posted May 14, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 15 Abstract 16 Some of the most burning questions in biology in recent years concern differential 17 diversification along the tree of life and its causes. -
Lycaste Lye-KASS-Tee
Lycaste lye-KASS-tee Lycastes are deciduous in various degrees, from the strongly deciduous, yellow-flowered species like Lycaste aromatica that flowers from leafless pseudobulbs to the evergreen types like Lycaste skinneri with pseudobulbs that retain their leaves at flowering. This genus produces large, long-lasting, showy, triangular flowers that are waxy. The plants are distinctive for their roundish pseudobulbs and broad, plicate (pleated) leaves. Culture for the hybrid genus Angulocaste (Lycaste ϫ Anguloa) follows the culture for the Lycaste parent. LIGHT requirements vary. Deciduous recommended during active growth species require light conditions as for cat- (usually summer); some growers spread tleyas — 2,000 to 4,000 foot-candles or 50 blood meal on the top of the potting medi- to 70 percent shade. More light is usually um as new pseudobulbs form, though in provided as new growths form pseudoulbs. inexperienced hands this can be dangerous Evergreen species grow best with less light to the plant. In autumn, or as growths — 1,500 to 2,000 foot-candles or 60 to 80 mature and pseudobulbs are produced, percent shade. fertilizer is reduced or switched to a high-phosphorus (such as 10-30-20) TEMPERATURES for the evergreen formulation to stimulate flower species should be fairly constant and never production. hot. Nights of 60 F and days of 75 to 80 F are desirable. The deciduous species of POTTING is best when new growth lycaste can tolerate a wider range, up to starts, usually in spring. A fine-grade pot- 95 F during the day and down to 50 F at ting medium is often used; fir bark and night when dormant in the winter. -
PC22 Doc. 22.1 Annex (In English Only / Únicamente En Inglés / Seulement En Anglais)
Original language: English PC22 Doc. 22.1 Annex (in English only / únicamente en inglés / seulement en anglais) Quick scan of Orchidaceae species in European commerce as components of cosmetic, food and medicinal products Prepared by Josef A. Brinckmann Sebastopol, California, 95472 USA Commissioned by Federal Food Safety and Veterinary Office FSVO CITES Management Authorithy of Switzerland and Lichtenstein 2014 PC22 Doc 22.1 – p. 1 Contents Abbreviations and Acronyms ........................................................................................................................ 7 Executive Summary ...................................................................................................................................... 8 Information about the Databases Used ...................................................................................................... 11 1. Anoectochilus formosanus .................................................................................................................. 13 1.1. Countries of origin ................................................................................................................. 13 1.2. Commercially traded forms ................................................................................................... 13 1.2.1. Anoectochilus Formosanus Cell Culture Extract (CosIng) ............................................ 13 1.2.2. Anoectochilus Formosanus Extract (CosIng) ................................................................ 13 1.3. Selected finished -
Eight New Species of Pleurothallidinae (Orchidaceae) ISSN-Internet 2195-772X 23.5.2018 Orchideenjournal Internet | Vol
OrchideenJournal Publisher: V.D.O.F. Vol. 6 · 3 Vereinigung Deutscher Orchideenfreunde e.V. 2018 Octomeria doucetteana Contents: Page 1 – 14 l Eight new species of Pleurothallidinae (Orchidaceae) ISSN-Internet 2195-772X 23.5.2018 OrchideenJournal Internet | Vol. 6 · 3 Internet | Vol. 6 · 3 OrchideenJournal Eight new species of Pleurothallidinae (Orchidaceae) Described from the collection of Alfonso Doucette Eight new species of Pleurothallidinae Luke E. Matthews 3 (Orchidaceae) Summary: A new species of Lepanthes is described as Lepanthes alphonsiana; a Described from the collection of Alfonso Doucette Luke E. Matthews new species of Lepanthopsis is described as Lepanthopsis maculanthina; a new species of Masdevallia is described as Masdevallia alphonsiana; a new species of Octomeria is described as Octomeria doucetteana; two new species of Phloe- Abstract: Recent investigation con- the pedicel (Dressler 1981). The spe- species discovered in the extraordi- ophila are described as Phloeophila alphonsiana and Phloeophila doucetteana; ducted by Luke M. Matthews in the cies are distributed across the neo- nary collection of Alfonso Doucette. a new species of Pleurothallis is described as Pleurothallis doucetteana; a new orchid collection of Alfonso Douc- tropics in a wide range of habitats species of Stelis is described as Stelis uvaegelata. ette Madison, WI, USA, have re- from sea level to over 4,000 m in el- Lepanthes alphonsiana L.E. vealed eight species of Pleurothal- evation (Pridgeon 2005). In those Matthews, sp. nov. Key words: Neotropics, Lepanthes alphonsiana, Lepanthopsis maculanthina, lidinae that include a unique species habitats, they have evolved a variety Type: HORTICULTURE. Without collec- Masdevallia alphonsiana, Octomeria doucetteana, Phloeophila alphonsiana, belonging to the Lepanthes sect. -
The Orchid Flora of the Colombian Department of Valle Del Cauca Revista Mexicana De Biodiversidad, Vol
Revista Mexicana de Biodiversidad ISSN: 1870-3453 [email protected] Universidad Nacional Autónoma de México México Kolanowska, Marta The orchid flora of the Colombian Department of Valle del Cauca Revista Mexicana de Biodiversidad, vol. 85, núm. 2, 2014, pp. 445-462 Universidad Nacional Autónoma de México Distrito Federal, México Available in: http://www.redalyc.org/articulo.oa?id=42531364003 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Revista Mexicana de Biodiversidad 85: 445-462, 2014 Revista Mexicana de Biodiversidad 85: 445-462, 2014 DOI: 10.7550/rmb.32511 DOI: 10.7550/rmb.32511445 The orchid flora of the Colombian Department of Valle del Cauca La orquideoflora del departamento colombiano de Valle del Cauca Marta Kolanowska Department of Plant Taxonomy and Nature Conservation, University of Gdańsk. Wita Stwosza 59, 80-308 Gdańsk, Poland. [email protected] Abstract. The floristic, geographical and ecological analysis of the orchid flora of the department of Valle del Cauca are presented. The study area is located in the southwestern Colombia and it covers about 22 140 km2 of land across 4 physiographic units. All analysis are based on the fieldwork and on the revision of the herbarium material. A list of 572 orchid species occurring in the department of Valle del Cauca is presented. Two species, Arundina graminifolia and Vanilla planifolia, are non-native elements of the studied orchid flora. The greatest species diversity is observed in the montane regions of the study area, especially in wet montane forest.