Sequence Based Prediction of Novel Domains in the Cellulosome of Ruminiclostridium Thermocellum
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A Parts List for Fungal Cellulosomes Revealed by Comparative Genomics Charles H
LETTERS PUBLISHED: 30 MAY 2017 | VOLUME: 2 | ARTICLE NUMBER: 17087 A parts list for fungal cellulosomes revealed by comparative genomics Charles H. Haitjema1, Sean P. Gilmore1,JohnK.Henske1, Kevin V. Solomon1†, Randall de Groot1, Alan Kuo2, Stephen J. Mondo2, Asaf A. Salamov2, Kurt LaButti2, Zhiying Zhao2, Jennifer Chiniquy2, Kerrie Barry2, Heather M. Brewer3,SamuelO.Purvine3,AaronT.Wright4, Matthieu Hainaut5,6, Brigitte Boxma7,TheovanAlen7†, Johannes H. P. Hackstein7, Bernard Henrissat5,6,8, Scott E. Baker3, Igor V. Grigoriev2,9 and Michelle A. O’Malley1* Cellulosomes are large, multiprotein complexes that tether enzyme transcripts yet found in nature7. The vast majority of plant biomass-degrading enzymes together for improved these enzymes carry non-catalytic dockerin domains (NCDDs), hydrolysis1. These complexes were first described in anaerobic which mediate assembly into large multiprotein complexes, or bacteria, where species-specific dockerin domains mediate the cellulosomes7. Cellulosomes were first described in anaerobic assembly of enzymes onto cohesin motifs interspersed within bacteria, and the modular interaction scheme native to these protein scaffolds1. The versatile protein assembly mechanism complexes has rapidly been exploited for biomass conversion and conferred by the bacterial cohesin–dockerin interaction is now enzyme tethering applications8–10. However, since the first report a standard design principle for synthetic biology2,3.For of cellulosome-like structures in anaerobic fungi more than decades, analogous structures have been reported in anaerobic twenty years ago11, the identification of dockerin-binding cohesins, fungi, which are known to assemble by sequence-divergent or scaffoldins that mediate assembly, has yet to be accomplished non-catalytic dockerin domains (NCDDs)4. However, the due to a lack of genomic data, functional proteomics and components, modular assembly mechanism and functional characterized strains. -
The Cellulosome Paradigm in an Extreme Alkaline Environment Paripok Phitsuwan, Sarah Moraïs, Bareket Dassa, Bernard Henrissat, Edward Bayer
The Cellulosome Paradigm in An Extreme Alkaline Environment Paripok Phitsuwan, Sarah Moraïs, Bareket Dassa, Bernard Henrissat, Edward Bayer To cite this version: Paripok Phitsuwan, Sarah Moraïs, Bareket Dassa, Bernard Henrissat, Edward Bayer. The Cellulo- some Paradigm in An Extreme Alkaline Environment. Microorganisms, MDPI, 2019, 7 (9), pp.347. 10.3390/microorganisms7090347. hal-02612575 HAL Id: hal-02612575 https://hal-amu.archives-ouvertes.fr/hal-02612575 Submitted on 19 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License microorganisms Article The Cellulosome Paradigm in An Extreme Alkaline Environment 1, 1,2 1 3,4 Paripok Phitsuwan y, Sarah Moraïs , Bareket Dassa , Bernard Henrissat and Edward A. Bayer 1,* 1 Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot 7610001, Israel 2 Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000, Israel 3 Architecture et Fonction des Macromolécules Biologiques, CNRS and Aix-Marseille University and CNRS, 13288 Marseille, France 4 USC1408, INRA, Architecture et Fonction des Macromolécules Biologiques, 13288 Marseille, France * Correspondence: [email protected] Present address: Division of Biochemical Technology, School of Bioresources and Technology, y King Mongkut’s University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand. -
Non-Cellulosomal Cohesin- and Dockerin-Like Modules in the Three Domains of Life Ayelet Peera, Steven P
1 Non-cellulosomal Cohesin- and Dockerin-like Modules in the Three Domains of Life Ayelet Peera, Steven P. Smithb, Edward A. Bayerc,*, Raphael Lameda and Ilya Borovoka aDepartment of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978 Israel bDepartment of Biochemistry, Queen’s University Kingston Ontario Canada K7L 3N6 cDepartment of Biological Sciences, Weizmann Institute of Science, Rehovot 76100 Israel *Corresponding author: Edward A. Bayer Tel: (+972) -8-934-2373 Fax: (+972)-8-946-8256 Email: [email protected] Supplementary Table S1. Compendium of cohesins and dockerins in the three domains of life. In order to discover new putative cohesin/dockerin-containing proteins, we used sequences of all the classical cohesin and dockerin modules from C. thermocellum, C. cellulovorans, C. cellulolyticum, B. cellulosolvens and Acetivibrio cellulolyticus as well as cohesins and dockerins recently discovered in rumen bacteria, Ruminococcus albus and R. flavefaciens as BlastP queries for the main NCBI Blast server against all non- redundant protein sequences deposited in GenBank/EMBL/DDBJ databases. We also performed extensive searches using the TblastN algorithm through all publicly available microbial genome databases including those attached to the NCBI BLAST server for bacterial genomes (http://www.ncbi.nlm.nih.gov/sutils/genom_table.cgi?), as well as several additional microbial genome databases – Microbial Genomics at the DOE Joint Genome Institute (http://genome.jgi-psf.org/mic_home.html), the Rumenomics database at TIGR/JCVI (http://tigrblast.tigr.org/rumenomics/index.cgi) and Bacterial Genomes at the Sanger Centre (http://www.sanger.ac.uk/Projects/Microbes/). Once a putative cohesin or dockerin-encoding gene product was identified, gene-walking techniques were employed to analyze and locate possible cellulosome-like gene clusters. -
A High Throughput Approach for the Generation of Orthogonally Interacting Protein Pairs Justin Lawrie University of Nebraska-Lincoln, [email protected]
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Faculty Publications -- Chemistry Department Published Research - Department of Chemistry 1-17-2018 A high throughput approach for the generation of orthogonally interacting protein pairs Justin Lawrie University of Nebraska-Lincoln, [email protected] Xi Song University of Nebraska-Lincoln Wei Niu University of Nebraska-Lincoln, [email protected] Jiantao Guo University of Nebraska-Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/chemfacpub Part of the Analytical Chemistry Commons, Medicinal-Pharmaceutical Chemistry Commons, and the Other Chemistry Commons Lawrie, Justin; Song, Xi; Niu, Wei; and Guo, Jiantao, "A high throughput approach for the generation of orthogonally interacting protein pairs" (2018). Faculty Publications -- Chemistry Department. 122. https://digitalcommons.unl.edu/chemfacpub/122 This Article is brought to you for free and open access by the Published Research - Department of Chemistry at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Faculty Publications -- Chemistry Department by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. www.nature.com/scientificreports OPEN A high throughput approach for the generation of orthogonally interacting protein pairs Received: 20 September 2017 Justin Lawrie1, Xi Song1, Wei Niu2 & Jiantao Guo1 Accepted: 27 December 2017 In contrast to the nearly error-free self-assembly of protein architectures in nature, artifcial assembly of Published: xx xx xxxx protein complexes with pre-defned structure and function in vitro is still challenging. To mimic nature’s strategy to construct pre-defned three-dimensional protein architectures, highly specifc protein- protein interacting pairs are needed. Here we report an efort to create an orthogonally interacting protein pair from its parental pair using a bacteria-based in vivo directed evolution strategy. -
Transcriptomic Characterization of Caecomyces Churrovis: a Novel, Non‑Rhizoid‑Forming Lignocellulolytic Anaerobic Fungus John K
Henske et al. Biotechnol Biofuels (2017) 10:305 https://doi.org/10.1186/s13068-017-0997-4 Biotechnology for Biofuels RESEARCH Open Access Transcriptomic characterization of Caecomyces churrovis: a novel, non‑rhizoid‑forming lignocellulolytic anaerobic fungus John K. Henske1, Sean P. Gilmore1, Doriv Knop1, Francis J. Cunningham1, Jessica A. Sexton1, Chuck R. Smallwood2, Vaithiyalingam Shutthanandan2, James E. Evans2, Michael K. Theodorou3 and Michelle A. O’Malley1* Abstract Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates. While most genera of gut fungi form extensive rhizoidal networks, which likely participate in mechanical disruption of plant cell walls, fungi within the Caecomyces genus do not possess these rhizoids. Here, we describe a novel fungal isolate, Caecomyces churrovis, which forms spherical sporangia with a limited rhizoidal network yet secretes a diverse set of carbohydrate active enzymes (CAZymes) for plant cell wall hydrolysis. Despite lacking an extensive rhizoidal system, C. churrovis is capable of growth on fbrous substrates like switchgrass, reed canary grass, and corn stover, although faster growth is observed on soluble sugars. Gut fungi have been shown to use enzyme complexes (fungal cellulosomes) in which CAZymes bind to non-catalytic scafoldins to improve bio- mass degradation efciency. However, transcriptomic analysis and enzyme activity assays reveal that C. churrovis relies more on free enzymes compared to other gut fungal isolates. Only 15% of CAZyme transcripts contain non-catalytic dockerin domains in C. churrovis, compared to 30% in rhizoid-forming fungi. Furthermore, C. churrovis is enriched in GH43 enzymes that provide complementary hemicellulose degrading activities, suggesting that a wider variety of these activities are required to degrade plant biomass in the absence of an extensive fungal rhizoid network. -
Open Surcelthesis.Pdf
The Pennsylvania State University The Graduate School The Huck Institutes of the Life Sciences INTERACTIONS BETWEEN THE COHESIN COMPLEX AND ITS DNA PARTNERS: A STRUCTURAL AND PHYLOGENETIC APPROACH A Thesis in Integrative Biosciences by Alexandra Surcel © 2007 Alexandra Surcel Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy August 2007 The thesis of Alexandra Surcel was reviewed and approved* by the following: Hong Ma Professor of Biology Thesis Advisor Chair of Committee David S. Gilmour Associate Professor of Molecular and Cell Biology William O. Hancock Assistant Professor of Bioengineering Wendy Hanna-Rose Assistant Professor of Molecular and Cell Biology Douglas Koshland Special member Senior staff member of the Carnegie Institution of Washington, Department of Embryology Investigator, Howard Hughes Medical Institute Peter J Hudson Director of the Integrative Biosciences Graduate Degree Program The Huck Institutes of the Life Sciences *Signatures are on file in the Graduate School iii ABSTRACT Cohesin is an evolutionarily conserved protein complex responsible for maintaining sister chromatid cohesion from early S phase to the metaphase-anaphase transition. The cohesin complex is comprised of four proteins – Smc1 and Smc3 that heterodimerize, and Scc1 and Scc3. Imaging of the Smc heterodimer shows that it forms a V shaped molecule and imaging of the entire cohesin holocomplex suggests that it forms an enclosed ring structure. The cohesin complex binds to specific loci along the chromosome arms and centromeres known as Cohesin Attachment Regions (CAR). In lieu of consensus binding sequences at CAR loci, several models have been proposed for cohesin interactions at CAR sites, though no direct structural information about the in vivo interaction of cohesin at CARs has been obtained. -
Subcellular Localization of Extracytoplasmic Proteins in Monoderm Bacteria: Rational Secretomics-Based Strategy for Genomic and Proteomic Analyses
Subcellular Localization of Extracytoplasmic Proteins in Monoderm Bacteria: Rational Secretomics-Based Strategy for Genomic and Proteomic Analyses Sandra Renier, Pierre Micheau, Re´gine Talon, Michel He´braud, Mickae¨l Desvaux* INRA, UR454 Microbiology, Saint-Gene`s Champanelle, France Abstract Genome-scale prediction of subcellular localization (SCL) is not only useful for inferring protein function but also for supporting proteomic data. In line with the secretome concept, a rational and original analytical strategy mimicking the secretion steps that determine ultimate SCL was developed for Gram-positive (monoderm) bacteria. Based on the biology of protein secretion, a flowchart and decision trees were designed considering (i) membrane targeting, (ii) protein secretion systems, (iii) membrane retention, and (iv) cell-wall retention by domains or post-translocational modifications, as well as (v) incorporation to cell-surface supramolecular structures. Using Listeria monocytogenes as a case study, results were compared with known data set from SCL predictors and experimental proteomics. While in good agreement with experimental extracytoplasmic fractions, the secretomics-based method outperforms other genomic analyses, which were simply not intended to be as inclusive. Compared to all other localization predictors, this method does not only supply a static snapshot of protein SCL but also offers the full picture of the secretion process dynamics: (i) the protein routing is detailed, (ii) the number of distinct SCL and protein categories is comprehensive, (iii) the description of protein type and topology is provided, (iv) the SCL is unambiguously differentiated from the protein category, and (v) the multiple SCL and protein category are fully considered. In that sense, the secretomics-based method is much more than a SCL predictor. -
Universidade De Lisboa Faculdade De Medicina Veterinária
UNIVERSIDADE DE LISBOA FACULDADE DE MEDICINA VETERINÁRIA DIVERGENT CELLULOSOME ARCHITECTURE IN RUMEN BACTERIA: STRUCTURE AND FUNCTION STUDIES IN COHESIN-DOCKERIN COMPLEXES OF RUMINOCOCCUS FLAVEFACIENS PEDRO MIGUEL BULE GOMES Orientadores: Doutor Carlos Mendes Godinho de Andrade Fontes Doutor Shabir Najmudin Tese especialmente elaborada para obtenção do grau de Doutor em Ciências Veterinárias na Especialidade de Produção Animal 2017 UNIVERSIDADE DE LISBOA FACULDADE DE MEDICINA VETERINÁRIA DIVERGENT CELLULOSOME ARCHITECTURE IN RUMEN BACTERIA: STRUCTURE AND FUNCTION STUDIES IN COHESIN-DOCKERIN COMPLEXES OF RUMINOCOCCUS FLAVEFACIENS PEDRO MIGUEL BULE GOMES Orientadores: Professor Doutor Carlos Mendes Godinho de Andrade Fontes Doutor Shabir Najmudin Tese especialmente elaborada para obtenção do grau de Doutor em Ciências Veterinárias na Especialidade de Produção Animal Júri: Presidente: Doutor Rui Manuel de Vasconcelos e Horta Caldeira Vogais: Doutor Harry J. Gilbert Doutor Gideon Davies Doutor Luís Manuel dos Anjos Ferreira Doutor José António Mestre Prates Doutor Carlos Mendes Godinho de Andrade Fontes 2017 Para a coautora das minhas aventuras Joana Dias I II Agradecimentos Ao entrar na reta final de uma das fases mais desafiantes da minha vida, não poderia deixar de aproveitar esta oportunidade para agradecer a todos os que estiveram envolvidos no meu percurso como estudante de doutoramento. Embora as grandes conquistas científicas estejam invariavelmente associadas a um nome, o sucesso científico está longe de ser um esforço individual. Uma equipa que nos apoie, motive e estimule a persistir mesmo nos momentos de maior frustração, é essencial para alcançar os nossos objetivos. Nesse aspeto considero-me bastante afortunado pois o grupo de trabalho a que pertenci ao longo destes anos foi fundamental para o meu sucesso como investigador. -
Ruminococcus Flavefaciens FD-1
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central Abundance and Diversity of Dockerin-Containing Proteins in the Fiber-Degrading Rumen Bacterium, Ruminococcus flavefaciens FD-1 Marco T. Rincon1., Bareket Dassa2., Harry J. Flint1*, Anthony J. Travis1, Sadanari Jindou3, Ilya Borovok3, Raphael Lamed3, Edward A. Bayer2, Bernard Henrissat4, Pedro M. Coutinho4, Dion A. Antonopoulos5, Margret E. Berg Miller5, Bryan A. White5 1 Microbial Ecology Group, Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, United Kingdom, 2 Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel, 3 Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel, 4 Architecture et Fonction des Macromolecules Biologiques, CNRS and Universites d’Aix-Marseille I & II, Marseille, France, 5 Division of Nutritional Sciences, Department of Animal Sciences, North American Consortium for Genomics of Fibrolytic Ruminal Bacteria, University of Illinois, Urbana, Illinois, United States of America Abstract Background: The cellulosome is a multi-enzyme machine, which plays a key role in the breakdown of plant cell walls in many anaerobic cellulose-degrading microorganisms. Ruminococcus flavefaciens FD-1, a major fiber-degrading bacterium present in the gut of herbivores, has the most intricate cellulosomal organization thus far described. Cellulosome complexes are assembled through high-affinity cohesin-dockerin interactions. More than two-hundred dockerin-containing proteins have been identified in the R. flavefaciens genome, yet the reason for the expansion of these crucial cellulosomal components is yet unknown. Methodology/Principal Findings: We have explored the full spectrum of 222 dockerin-containing proteins potentially involved in the assembly of cellulosome-like complexes of R. -
Complexity of the Ruminococcus Flavefaciens FD-1 Cellulosome Reflects an Expansion of Family- Related Protein-Protein Interactions
Israeli-Ruimy V, Bule P, Jindou S, Dassa B, Morais S, Borovok I, Barak Y, Slutzki M, Hamberg Y, Cardoso V, Alves VD, Najmudin S, White BA, Flint HJ, Gilbert HJ, Lamed R, Fontes CMGA, Bayer EA. Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family- related protein-protein interactions. Scientific Reports 2017, 7, 42355. Copyright: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material DOI link to article: http://dx.doi.org/10.1038/srep42355 Date deposited: 23/03/2017 This work is licensed under a Creative Commons Attribution 4.0 International License Newcastle University ePrints - eprint.ncl.ac.uk www.nature.com/scientificreports OPEN Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family- Received: 21 October 2016 Accepted: 08 January 2017 related protein-protein interactions Published: 10 February 2017 Vered Israeli-Ruimy1,*, Pedro Bule2,*, Sadanari Jindou3,†, Bareket Dassa1, Sarah Moraïs1, Ilya Borovok3, Yoav Barak1,4, Michal Slutzki1, Yuval Hamberg1, Vânia Cardoso2, Victor D. Alves2, Shabir Najmudin2, Bryan A. White5,6, Harry J. Flint7, Harry J. Gilbert8, Raphael Lamed3, Carlos M. G. A. Fontes2 & Edward A. Bayer1 Protein-protein interactions play a vital role in cellular processes as exemplified by assembly of the intricate multi-enzyme cellulosome complex. -
A Journey from Cohesin-Dockerin Expression to Novel Carbohydrate Binding Module Structures
ANA JOSÉ DE OLIVEIRA NUNES PIRES BREAKING THE WALL! DECONSTRUCTION OF THE CELULLOSOME - A JOURNEY FROM COHESIN-DOCKERIN EXPRESSION TO NOVEL CARBOHYDRATE BINDING MODULE STRUCTURES Orientador: Professor Doutor Pedro Sampaio Universidade Lusófona de Humanidades e Tecnologias Faculdade de Engenharia Lisboa 2015 ANA JOSÉ DE OLIVEIRA NUNES PIRES BREAKING THE WALL! DECONSTRUCTION OF THE CELULLOSOME - A JOURNEY FROM COHESIN DOCKERIN EXPRESSION TO NOVEL CARBOHYDRATE BINDING MODULE STRUCTURES Dissertação defendida em provas públicas na Universidade Lusófona de Humanidades e Tecnologias no dia 22/11/2016, perante o júri, nomeado pelo Despacho de Nomeação n.º: 406/2016, de 4 de Novembro, com a seguinte composição: Presidente: Prof. Doutora Adília Charmier Arguente: Prof. Doutora Cecília Calado (ISEL) Orientador: Prof. Doutor Pedro Sampaio Universidade Lusófona de Humanidades e Tecnologias Faculdade de Engenharia Lisboa 2015 Ana José de Oliveira Nunes Pires - Breaking the wall! Deconstruction of the Cellulosome - A journey from cohesin dockerin expression to novel carbohydrate binding module structures To my Super Women: Mom, Aunt and Grandmother And my Super Heroes: Dad and Brother Universidade Lusófona de Humanidades e Tecnologias – Faculdade de Engenharia i Ana José de Oliveira Nunes Pires - Breaking the wall! Deconstruction of the Cellulosome - A journey from cohesin dockerin expression to novel carbohydrate binding module structures Acknowledgments “I saw the light fade from I turn at last to paths that By silver streams that run the sky lead -
Complexity of the Ruminococcus Flavefaciens FD-1
www.nature.com/scientificreports OPEN Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family- Received: 21 October 2016 Accepted: 08 January 2017 related protein-protein interactions Published: 10 February 2017 Vered Israeli-Ruimy1,*, Pedro Bule2,*, Sadanari Jindou3,†, Bareket Dassa1, Sarah Moraïs1, Ilya Borovok3, Yoav Barak1,4, Michal Slutzki1, Yuval Hamberg1, Vânia Cardoso2, Victor D. Alves2, Shabir Najmudin2, Bryan A. White5,6, Harry J. Flint7, Harry J. Gilbert8, Raphael Lamed3, Carlos M. G. A. Fontes2 & Edward A. Bayer1 Protein-protein interactions play a vital role in cellular processes as exemplified by assembly of the intricate multi-enzyme cellulosome complex. Cellulosomes are assembled by selective high-affinity binding of enzyme-borne dockerin modules to repeated cohesin modules of structural proteins termed scaffoldins. Recent sequencing of the fiber-degradingRuminococcus flavefaciens FD-1 genome revealed a particularly elaborate cellulosome system. In total, 223 dockerin-bearing ORFs potentially involved in cellulosome assembly and a variety of multi-modular scaffoldins were identified, and the dockerins were classified into six major groups. Here, extensive screening employing three complementary medium- to high-throughput platforms was used to characterize the different cohesin-dockerin specificities. The platforms included (i) cellulose-coated microarray assay, (ii) enzyme-linked immunosorbent assay (ELISA) and (iii) in-vivo co-expression and screening in Escherichia coli. The data revealed a collection of unique cohesin-dockerin interactions and support the functional relevance of dockerin classification into groups. In contrast to observations reported previously, a dual-binding mode is involved in cellulosome cell-surface attachment, whereas single-binding interactions operate for cellulosome integration of enzymes.