bioRxiv preprint doi: https://doi.org/10.1101/2020.07.27.223420; this version posted July 27, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 A genome-wide CRISPR/Cas phenotypic screen for 2 modulators of DUX4 cytotoxicity reveals screen 3 complications 4 5 Ator Ashoti1*, Francesco Limone2,5*, Melissa van Kranenburg1, Anna 6 Alemany1, Mirna Baak1, Judith Vivié1,3, Federica Piccioni8, Menno 7 Creyghton1,6, Kevin Eggan2,5**, Niels Geijsen1,4** 8 9 1Hubrecht Institute, Developmental Biology and Stem Cell Research, 10 Utrecht, Netherlands 11 2Department of Stem Cell and Regenerative Biology, Harvard University 12 Cambridge, MA, USA 13 5Stanley Center for Psychiatric Research, Broad Institute of MIT and 14 Harvard, Cambridge, MA, USA 15 3Single Cell Discoveries, Utrecht, Netherlands 16 4Leiden University Medical Center, Leiden, The Netherlands 17 6Erasmus University Medical Centre, Rotterdam, The Netherlands 18 8Broad institute, Cambridge, MA, USA 19 20 * Equal contribution 21 **Correspondence:
[email protected],
[email protected] 22 23 Acknowledgements 24 This study was supported by Stichting FSHD and the SingelSwim Utrecht. The authors 25 like to thank Nune Schelling, Peng Shang, Stefan van der Elst, en Reinier van der 26 Linden for excellent technical assistance. 27 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.27.223420; this version posted July 27, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.