LUCRARI STIINTIFICE MEDICINA VETERINARA VOL. XLIII (1), 2010 TIMISOARA IMMUNOLOGICAL DIAGNOSIS OF FASCIOLOSIS BY ELISA METHOD IN SHEEP AND CATTLE FROM ARAD COUNTY I. OPRESCU¹, GH. DARABU޹, S. MORARIU¹, NARCISA MEDERLE¹, M.S. ILIE¹, MIRELA IMRE¹, K. IMRE¹, P. ONITA² ¹Faculty of Veterinary Medicine Timisoara, 300645, Calea Aradului, No. 119, Timisoara, Romania ²DSVSA ARAD E-mail:
[email protected] Summary The aim of the study " Immunological diagnosis of fasciolosis by Elisa method in sheep and cattle from Arad County " was to make a serological screening in sheep and cattle from Arad County in 2008. Results obtained in sheep show a medium infestation of animals from six localities and a lower infestation in other 13 CSV units. ELISA test in bovines painted out negative results in four units, in three units the infestation was medium and in the other 12 units infestation was low. Based on the obtained results the extensivity of faciolosis was determined, and a mapping of this helminthosis in Arad County was made. Key words: immunological, diagnosis, fasciolosis Fasciolosis is one of the most important helmintosis affecting the liver, of ruminants and produces significant losses in livestock populations (7). Early diagnosis of fasciolosis creates the opportunity of a prompt intervention with narrow-spectrum products, but with a high degree of efficiency, so that economic losses are minimal (3, 8, 9, 10, 11, 15, 16, 17). The surveillance program implemented by A.N.S.V.S.A. is trying to perform the serological monitoring of sheep and cattle for Fasciola hepatica infestations. ELISA immunoassay method is a paraclinic sensitive and accurate test, being used in most Western laboratories to monitorize the infections in sheep and cattle in endemic areas (1, 4, 6, 13, 14).