Bt BRINJAL Primer
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Genetically Modified Cotton
BACKGROUND 2013 GENETICALLY MODIFIED COTTON Genetically engineered (also called genetically modified or GM) cotton is currently grown on 25 million hectares around the world, mostly in India, China, Pakistan and the US.1 Other countries growing much smaller amounts of GM cotton are South Africa, Burkina Faso, Sudan, Brazil, Argentina, Paraguay, Columbia, Mexico, Costa Rica, Burma, Australia, and Egypt. GM cotton is engineered with one of two traits. One makes it resistant to glyphosate-based herbicides such as Monsanto’s Roundup, SUMMARY while the other stimulates the plant to produce a toxin that kills the bollworm, one of the crop’s primary pests. This pest-resistant cotton GM COTTON FAILED is engineered with a gene from the bacteria Bacillus thurengiensis FARMERS IN INDIA or “Bt”, and is the more commonly grown of the two types. » Yields declined. BT COTTON IN INDIA » Secondary pests emerged, Cotton is an important cash crop in India. It is grown on 12 million forcing increased pesticide use. hectares, making India the second largest producer of cotton in the » The price of cotton seed rose. world, behind China. Insect-resistant Bt cotton is the only GM crop currently grown in India.2 It was introduced in India by Monsanto in » Farmers lost the option to buy 2002, under the trade name Bollgard, in a joint venture with the non-GM cotton seeds. Indian seed company Mahyco. Monsanto promised Indian farmers that Bt cotton would: 1. Reduce the amount of pesticides farmers need to buy to control pests, 2. Increase harvests and farm income by reducing crop losses due to pest attacks.3 In the first few years after Bt cotton was commercialized in India, some farmers saw reductions in pesticide use and crop losses, but this pattern quickly and dramatically changed. -
Bt Cotton in Texas
B-6107 02-01 Bt Cotton Technology in Texas: A Practical View Glen C. Moore, Thomas W. Fuchs, Mark A. Muegge, Allen E. Knutson* Since their introduction in 1996, transgenic cot- tons expressing the Bollgard® gene technology have been evaluated by producers in large scale commer- cial plantings across the U.S. Cotton Belt. Transgenic cottons are designed to be resistant to the target pests of bollworm Helicoverpa zea (Boddie), pink bollworm Pectinophora gossypiella (Sanders), and tobacco budworm Heliothis virescens (F.). These cottons contain Bacillus thuringiensis (Bt), a gene toxic to the target pests. The perfor- mance of these cottons has been highly efficacious against the tobacco budworm and the pink bollworm. They also preform well against bollworm; however, in certain situations producers may need to make supplemental insecticide treatments for this insect. Conditions that have contributed to the need for sup- plemental control are heavy bollworm egg laying during peak bloom, boll injury and the presence of larvae larger than 1/4 inch, high production inputs that favor rapid or rank plant growth, and fields pre- viously treated with insecticides. Earliest reports of bollworm damage on transgenic cotton varieties NuCOTN 33B and NuCOTN 35B surfaced in the Brazos Bottomlands and parts of the upper Coastal Bend areas of Texas in 1996. NuCOTN 33B and NuCOTN 35B have, however, provided effective bollworm control throughout much of Texas and reduced insecticide treatments for bollworm, tobacco budworm and pink bollworm compared to non-Bollgard® cotton. Yields from Bollgard® cotton are generally equal to or slightly higher than those for standard non- Bollgard® cultivars grown under the same production scheme. -
TESTS of the Associanon of HEAT SHOCK PROTEIN 90 with A
TESTS OF THE ASSOCIAnON OF HEAT SHOCK PROTEIN 90 WITH A CAULIFLOWER MOSAIC VIRUS REVERSE TRANSCRIPTASE By BRESHANA QUIENE JOHNSON Bachelor of Science Xavier University of Louisiana New Orleans, Louisiana May 1997 Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of MASTER OF SCIENCE July, 2000 Ok/ahoma State University Library TESTS OF THE ASSOCIAnON OF HEAT SHOCK PROTEIN 90 WITH A CAULIFLOWER MOSAIC VIRUS REVERSE TRANSCRIPTASE Thesis Approved: ~~-- Thesis Advisor ~~-~---- 11 ACKNOWLEDGEMENTS I sincerely thank my thesis advisor, Dr. Ulrich K. Melcher, for his guidance, supervision, expertise, and friendship. It was a pleasure to be a part of his Jab and I will always appreciate his dedication to his students and encouragement. I thank my committee members Dr. Richard C. Essenberg and Dr. Robelt L. Matts for their support and expertise. I also thank Dr. James B. Blair and the Department of Biochemistry and Molecular Biology for providing me with the opportunity to participate in the NIH Biomedical Graduate Students program and their financial support. I express my sincere gratitude to Dr. Steven D. Hartson for his guidance, suggestions, and inspiration throughout this project. I would like to thank staff and students of the Department of Biochemistry and Molecular Biology, who provided me with assistance: Ann Williams, Dr. Ba am Fraij, Dr. Jerry Merz, Wenhao Wang, Qi Jiang, Kenji Onodera, Wenjun Huang, Jieya Shao, Abdel Bior, and Thomas Prince I truly thank my parents for encouraging and supporting me throughout my academic pursuits. I am very grateful to them and blessed to have their love and concern. -
Barriers to Adoption of GM Crops
Iowa State University Capstones, Theses and Creative Components Dissertations Fall 2021 Barriers to Adoption of GM Crops Madeline Esquivel Follow this and additional works at: https://lib.dr.iastate.edu/creativecomponents Part of the Agricultural Education Commons Recommended Citation Esquivel, Madeline, "Barriers to Adoption of GM Crops" (2021). Creative Components. 731. https://lib.dr.iastate.edu/creativecomponents/731 This Creative Component is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Creative Components by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Barriers to Adoption of GM Crops By Madeline M. Esquivel A Creative Component submitted to the Graduate Faculty in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Major: Plant Breeding Program of Study Committee: Walter Suza, Major Professor Thomas Lübberstedt Iowa State University Ames, Iowa 2021 1 Contents 1. Introduction ................................................................................................................................. 3 2. What is a Genetically Modified Organism?................................................................................ 9 2.1 The Development of Modern Varieties and Genetically Modified Crops .......................... 10 2.2 GM vs Traditional Breeding: How Are GM Crops Produced? -
Non-Recessive Bt Toxin Resistance Conferred by an Intracellular Cadherin Mutation in Field-Selected Populations of Cotton Bollworm
Non-Recessive Bt Toxin Resistance Conferred by an Intracellular Cadherin Mutation in Field-Selected Populations of Cotton Bollworm Haonan Zhang1, Shuwen Wu1, Yihua Yang1, Bruce E. Tabashnik2, Yidong Wu1* 1 Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China, 2 Department of Entomology, University of Arizona, Tucson, Arizona, United States of America Abstract Transgenic crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce the benefits of this approach. Recessive mutations in the extracellular domain of toxin- binding cadherin proteins that confer resistance to Bt toxin Cry1Ac by disrupting toxin binding have been reported previously in three major lepidopteran pests, including the cotton bollworm, Helicoverpa armigera. Here we report a novel allele from cotton bollworm with a deletion in the intracellular domain of cadherin that is genetically linked with non- recessive resistance to Cry1Ac. We discovered this allele in each of three field-selected populations we screened from northern China where Bt cotton producing Cry1Ac has been grown intensively. We expressed four types of cadherin alleles in heterologous cell cultures: susceptible, resistant with the intracellular domain mutation, and two complementary chimeric alleles with and without the mutation. Cells transfected with each of the four cadherin alleles bound Cry1Ac and were killed by Cry1Ac. However, relative to cells transfected with either the susceptible allele or the chimeric allele lacking the intracellular domain mutation, cells transfected with the resistant allele or the chimeric allele containing the intracellular domain mutation were less susceptible to Cry1Ac. -
CRISPR/Cas9-Mediated Resistance to Cauliflower Mosaic Virus Haijie Liu1
bioRxiv preprint doi: https://doi.org/10.1101/191809; this version posted September 23, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. CRISPR/Cas9-mediated resistance to cauliflower mosaic virus Haijie Liu1,*, Cara L. Soyars2,7, *, Jianhui Li1, *, Qili Fei4,5, Guijuan He1, Brenda A. Peterson2,7, Blake C. Meyers4,5,6, Zachary L. Nimchuk2,3,7,8, and Xiaofeng Wang1,#. 1Department of Plant Pathology, Physiology and Weed Science; 2Department of Biological Sciences; 3Faculty of Health Sciences; Virginia Tech, Blacksburg, VA, USA; 4Department of Plant & Soil Sciences and Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA; 5Donald Danforth Plant Science Center, St. Louis, Missouri, USA; 6University of Missouri – Columbia, Division of Plant Sciences, Columbia, MO; 7Department of Biology, University of North Carolina at Chapel Hill, and 8Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; *These authors contributed equally to this project. # Author for correspondence: Xiaofeng Wang, 549 Latham Hall, 220 Ag Quad Lane, Blacksburg, VA 24061. Telephone: 1-540-231-1868, Fax: 1-540-231-7477, Email: [email protected]. H. Liu: [email protected]; C.L. Soyars: [email protected]; J. Li: [email protected]; Q. Fei: [email protected]; G. He: [email protected]; B.A. Peterson: [email protected]; B.C. Meyers: [email protected]; Z.L. Nimchuk: [email protected] Running title: CRISPR-Cas9-conferred resistance to CaMV Key words: CRISPR-Cas9, cauliflower mosaic virus, virus resistance, virus escape, small RNA Word count: Summary, 216 and text, 4201 1 bioRxiv preprint doi: https://doi.org/10.1101/191809; this version posted September 23, 2017. -
Monsanto's BT Cotton Patent, Indian Courts and Public Policy
6. MONSANTO’S BT COTTON PATENT, INDIAN COURTS AND eligible subject matter promotes public policy and farmers’ PUBLIC POLICY interests. Keywords: Bt. cotton, patent eligible subject matter, nucleic Ghayur Alam∗ acid sequence, plant, public policy, revocation of patent, seed, ABSTRACT TRIPS. This Paper primarily deals with an unanswered substantial 1. INTRODUCTION question of patent law that has arisen in India. The question is whether an invented Nucleic Acid Sequence after being A substantial question of patent law (hereinafter, ‘question’) inserted into a seed or plant becomes part of the seed or the of great significance has arisen in India and is awaiting an plant. If answer is in the affirmative, said invention is not answer from Indian courts. The question is whether an patentable under Section 3(j) of the (Indian) Patents Act 1970, invented Nucleic Acid Sequence (NAS) after being inserted which excludes from patentability, inter alia, plants, seeds, or into a seed or plant becomes part of the seed or the plant? any part thereof. If answer is in the negative, said invention is Specifically, the question is whether the patent granted1 by patentable. Answer will determine the fate of patenting of the Indian Patent Office to Monsanto Technology LLC such inventions in the field of agro-biotechnology. Problem is (Monsanto) on transgenic variety of cottonseeds, containing that the question has moved forth and back like pendulum invented Bt. trait is valid or not? If the answer is in affirmative, from one court to another but in vain. This paper seeks to claimed invention is not patentable under the (Indian) address this question in light of the decisions of Indian courts. -
Resistance Management Plan (Rmp) Resistance Management 1
BOLLGARD® 3 RESISTANCE MANAGEMENT PLAN (RMP) RESISTANCE MANAGEMENT 1. PLANTING RESTRICTIONS Victoria, New South Wales and Southern PLAN Queensland Developed by Monsanto Australia Pty Ltd. All Bollgard 3 crops and refuges must be planted into moisture or watered-up between August 1 and before December 31 each year, The Resistance Management Plan is unless otherwise specified in this Resistance Management Plan. based on three basic principles: (1) Central Queensland minimising the exposure of Helicoverpa All Bollgard 3 crops and refuges must be planted into moisture or watered-up between August 1 and before October 31 each spp. to the Bacillus thuringiensis year, unless otherwise specified in this Resistance Management (Bt) proteins Cry1Ac, Cry2Ab and Plan. Bollgard 3 can only be planted from August 1 to October 31 each year. Seed cannot be planted wet or dry prior to August 1. Vip3A, (2) providing a population of Any Bollgard 3 crops planted into moisture or watered-up after susceptible individuals that can mate October 31 and up to December 31 must plant additional refuge as specified in Table 3 and 4. Bollgard 3 cannot be planted dry with any resistant individuals, hence prior to December 31 if not watered up. diluting any potential resistance, and 2. REFUGES (3) removing resistant individuals at the end of the cotton season. These principles are Growers planting Bollgard 3 cotton will be required to grow a refuge crop that is capable of producing large numbers of Helicoverpa supported through the implementation of spp. moths which have not been exposed to selection with the five elements that are the key components Bt proteins Cry1Ac, Cry2Ab and Vip3A. -
Cauliflower Mosaic Virus (Camv)
Cauliflower Mosaic Virus (CaMV) Dr.Ramesh C.K Cauliflower Mosaic Virus (CaMV) • Cauliflower mosaic virus (CaMV) is a member of the genus Caulimovirus, one of the six genera in the family Caulimoviridae which are pararetroviruses that infect plants. • Pararetroviruses group due to its mode of replication via reverse transcription of a pre- genomic RNA intermediate. just like retroviruses but the viral particles contain DNA instead of RNA. • True retroviruses are not known in plants; however, plant pararetroviruses (caulimoviridae) share many retroviral properties, replicating by transcription in the nucleus followed by reverse transcription in the cytoplasm. • Pararetroviruses have circular DNA genomes that do not integrate into the host genome, and display several unique expression strategies. • CaMV infects mostly plants of the family Brassicaceae (such as cauliflower and turnip) but some CaMV strains are also able to infect Solanaceae species of the genera Datura and Nicotiana. • CaMV induces a variety of systemic symptoms such as mosaic, necrotic lesions on leaf surfaces, stunted growth, and deformation of the overall plant structure. • CaMV is transmitted by aphid species such as Myzus persicae. Once introduced within a plant host cell, virions migrate to the nuclear envelope of the plant cell. Structure • The CaMV particle is an icosahedron with a diameter of 52 nm built from 420 capsid protein (CP), which surrounds a solvent-filled central cavity. • In addition to capsid proteins, caulimoviruses are also surrounded by virus associated proteins. These proteins are responsible for assisting in the binding of the virus to DNA • CaMV contains a circular double-stranded DNA molecule of about 8.0 kilobases, interrupted by nicks that result from the actions of RNAse H during reverse transcription. -
Diversity of Insects Under the Effect of Bt Maize and Insecticides Diversidade De Insetos Sob a Influência Do Milho Bt E Inseticidas
AGRICULTURAL ENTOMOLOGY / SCIENTIFIC ARTICLE DOI: 10.1590/1808-1657000062015 Diversity of insects under the effect of Bt maize and insecticides Diversidade de insetos sob a influência do milho Bt e inseticidas Marina Regina Frizzas1*, Charles Martins de Oliveira2, Celso Omoto3 ABSTRACT: The genetically modified maize to control RESUMO: O milho geneticamente modificado visando ao controle some caterpillars has been widely used in Brazil. The effect de lagartas tem sido amplamente utilizado no Brasil. Em estudo de of Bt maize and insecticides was evaluated on the diversity of campo realizado em Ponta Grossa (Paraná, Brasil), compararam-se, insects (species richness and abundance), based on the insect com base na diversidade (riqueza de espécies e abundância), os efeitos community, functional groups and species. This study was do milho Bt e do controle químico sobre a comunidade de insetos, conducted in genetically modified maize MON810, which grupos funcionais e espécies. A comunidade de insetos foi amostrada expresses the Cry1Ab protein from Bacillus thuringiensis Berliner, no milho geneticamente modificado MON810, que expressa a proteína and conventional maize with and without insecticide sprays Cry1Ab de Bacillus thuringiensis Berliner, e no milho convencional (lufenuron and lambda-cyhalothrin) under field conditions com e sem a aplicação de inseticidas (lufenuron e lambda-cialotrina). in Ponta Grossa (Paraná state, Brazil). Insect samplings were As amostragens foram realizadas por meio da coleta de insetos utili- performed by using pitfall trap, water tray trap and yellow sticky zando-se armadilha de queda, bandeja-d’água e cartão adesivo. Foram card. A total of 253,454 insects were collected, distributed coletados 253.454 insetos, distribuídos em nove ordens, 82 famílias among nine orders, 82 families and 241 species. -
Transposon Insertion Causes Cadherin Mis-Splicing and Confers Resistance
www.nature.com/scientificreports OPEN Transposon insertion causes cadherin mis-splicing and confers resistance to Bt cotton in pink Received: 7 August 2018 Accepted: 2 May 2019 bollworm from China Published: xx xx xxxx Ling Wang1,2, Jintao Wang1,3, Yuemin Ma4, Peng Wan1, Kaiyu Liu4, Shengbo Cong1, Yutao Xiao5, Dong Xu1, Kongming Wu2, Jefrey A. Fabrick 6, Xianchun Li7 & Bruce E. Tabashnik 7 Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) are cultivated extensively, but rapid evolution of resistance by pests reduces their efcacy. We report a 3,370- bp insertion in a cadherin gene associated with resistance to Bt toxin Cry1Ac in the pink bollworm (Pectinophora gossypiella), a devastating global cotton pest. We found the allele (r15) harboring this insertion in a feld population from China. The insertion is a miniature inverted repeat transposable element (MITE) that contains two additional transposons and produces two mis-spliced transcript variants (r15A and r15B). A strain homozygous for r15 had 290-fold resistance to Cry1Ac, little or no cross-resistance to Cry2Ab, and completed its life cycle on Bt cotton producing Cry1Ac. Inheritance of resistance was recessive and tightly linked with r15. For transformed insect cells, susceptibility to Cry1Ac was greater for cells producing the wild-type cadherin than for cells producing the r15 mutant proteins. Recombinant cadherin protein occurred on the cell surface in cells transformed with the wild- type or r15A sequences, but not in cells transformed with the r15B sequence. The similar resistance of pink bollworm to Cry1Ac in laboratory- and feld-selected insects from China, India and the U.S. -
Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond Mikhail M
Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond Mikhail M. Pooggin, Lyuba Ryabova To cite this version: Mikhail M. Pooggin, Lyuba Ryabova. Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond. Frontiers in Microbiology, Frontiers Media, 2018, 9, pp.644. 10.3389/fmicb.2018.00644. hal-02289592 HAL Id: hal-02289592 https://hal.archives-ouvertes.fr/hal-02289592 Submitted on 16 Sep 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - ShareAlike| 4.0 International License fmicb-09-00644 April 9, 2018 Time: 16:25 # 1 REVIEW published: 10 April 2018 doi: 10.3389/fmicb.2018.00644 Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond Mikhail M. Pooggin1* and Lyubov A. Ryabova2* 1 INRA, UMR Biologie et Génétique des Interactions Plante-Parasite, Montpellier, France, 2 Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, Strasbourg, France Viruses have compact genomes and usually translate more than one protein from polycistronic RNAs using leaky scanning, frameshifting, stop codon suppression or reinitiation mechanisms.