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ACTL8 (H): 293T Lysate: Sc-113876

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ACTL8 (H): 293T Lysate: Sc-113876 SAN TA C RUZ BI OTEC HNOL OG Y, INC . ACTL8 (h): 293T Lysate: sc-113876 BACKGROUND APPLICATIONS ACTL8 (Actin-like protein 8), aslo known as CT57 (cancer/testis antigen 57), ACTL8 (h): 293T Lysate is suitable as a Western Blotting positive control for is a member of the ARP family of Actin-related proteins that contain an Actin human reactive ACTL8 antibodies. Recommended use: 1 0-20 µl per lane. fold and are involved in spindle orientation, nuclear migration and chromatin Control 293T Lysate: sc-117752 is available as a Western Blotting negative remodeling events. Localized to the cytoplasm and expressed in the testis control lysate derived from non-transfected 293T cells. and pancreas, ACTL8 is 366 amino acids in length and is encoded by a gene that maps to human chromosome 1, which spans 260 million base pairs, ACTL8 (A-4): sc-377372 is recommended as a positive control antibody for contains over 3,000 genes and comprises nearly 8% of the human genome. Western Blot analysis of enhanced human ACTL8 expression in ACTL8 trans - Chromosome 1 houses a large number of disease-associated genes, including fected 293T cells (starting dilution 1:100, dilution range 1:100-1:1,000). those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. RECOMMENDED SUPPORT REAGENTS Aberrations in chromosome 1 are found in a variety of cancers, including To ensure optimal results, the following support reagents are recommended: head and neck cancer, malignant melanoma and multiple myeloma. 1) Western Blotting: use m-IgG κ BP-HRP: sc-516102 or m-IgG κ BP-HRP (Cruz Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ REFERENCES Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: 1. Blackwood, D.H., Fordyce, A., Walker, M.T., St. Clair, D.M., Porteous, D.J. sc-516214 and Western Blotting Luminol Reagent: sc-2048. and Muir, W.J. 2001. Schizophrenia and affective disorders—cosegrega - tion with a translocation at chromosome 1q42 that directly disrupts brain- DATA expressed genes: clinical and P300 findings in a family. Am. J. Hum. Genet. 69: 428-433. AB AB 90K– 2. Weise, A., Starke, H., Mrasek, K., Claussen, U. and Liehr, T. 2005. New 114 K – insights into the evolution of chromosome 1. Cytogenet. Genome Res. 55K– 85K– 108: 217-222. 43K– < ACTL8 34K– 49K– 3. Chen, Y.T., Scanlan, M.J., Venditti, C.A., Chua, R., Theiler, G., Stevenson, < ACTL8 B.J., Iseli, C., Gure, A.O., Vasicek, T., Strausberg, R.L., Jongeneel, C.V., Old, L.J. and Simpson, A.J. 2005. Identification of cancer/testis-antigen genes by massively parallel signature sequencing. Proc. Natl. Acad. Sci. ACTL8 (A-4): sc-377372. Western blot analysis of ACTL8 (A-4): sc-377372. Near-infrared western ACTL8 expression in non-transfected: sc-117752 (A) blot analysis of ACTL8 expression in non-transfected: USA 102: 7940-7945. and human ACTL8 transfected: sc-113876 (B) 293T sc-117752 (A) and human ACTL8 transfected: whole cell lysates. sc-113876 (B) 293T whole cell lysates. Blocked with 4. Marzin, Y., Jamet, D., Douet-Guilbert, N., Morel, F., Le Bris, M.J., Morice, UltraCruz® Blocking Reagent: sc-516214. Detection P., Abgrall, J.F., Berthou, C. and De Braekeleer, M. 2006. Chromosome 1 reagent used: m-IgGκ BP-CFL 680: sc-516180. abnormalities in multiple myeloma. Anticancer Res. 26: 953-959. RESEARCH USE 5. Gregory, S.G., Barlow, K.F., McLay, K.E., Kaul, R., Swarbreck, D., Dunham, For research use only, not for use in diagnostic procedures. A., Scott, C.E., Howe, K.L., Woodfine, K., Spencer, C.C., Jones, M.C., Gillson, C., Searle, S., Zhou, Y., et al. 2006. The DNA sequence and biological annotation of human chromosome 1. Nature 441: 315-321. CHROMOSOMAL LOCATION Genetic locus: ACTL8 (human) mapping to 1p36.13. PRODUCT ACTL8 (h): 293T Lysate represents a lysate of human ACTL8 transfected 293T cells and is provided as 100 µg protein in 200 µl SDS-PAGE b uffer. STORAGE Store at -20° C. Repeated freezing and thawing should be minimized. Sample vial should be boiled once prior to use. Non-hazardous. No MSDS required. PROTOCOLS See our web site at www.scbt.com for detailed protocols and support products. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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