Genus Heliocopris Hope, 1837
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SCARABS lg fx mz gq om vf yw bq iz sl ks wu Occasional Issue Number 83 Print ISSN 1937-8343 Online ISSN 1937-8351 March, 2017 Focus-Stacking Macro Photography System WITHIN THIS ISSUE for Around $1,200 Focus Stacking System .... 1 by Chris Moeseneder Collecting Heliocopris ..... 8 CSIRO Oceans and Atmosphere Flagship Ecosciences Precinct, Dutton Park Flight Intercept Trap ..... 15 Queensland, Australia, [email protected] The challenge was to build a a private budget it is also a solution focus-stacking macrophotography which will not blow your project system as cost-effective as possible budget and may just slip under the while still ensuring that it performs threshold of a capital investment. well. This article presents the There are many camera, lighting, system which I built for this stage and software manufacturers purpose, complete instructions which produce components that on how to replicate and operate it, are as good, or even better, than and its advantages, drawbacks and those mentioned in this article. calibration. The skills required to However, the combination of BACK ISSUES build the system are low and tools products presented here has been Available At These Sites: are few but you must be able to successfully assembled into a Coleopterists Society read a technical drawing and use reliable, replicable, cost-effective www.coleopsoc.org/de- power tools. While this system was and easy to operate system. fault.asp?Action=Show_ developed under the constraints of Resources&ID=Scarabs University of Nebraska www-museum.unl.edu/ research/entomology/ Scarabs-Newsletter.htm EDITORS Rich Cunningham [email protected] Olivier Décobert [email protected] Barney Streit Figure 1: Ischiopsopha wallacei. Mostly out of focus. barneystreit@hotmail. com Problems of Photography at Small Scales In photography at small scale - the size of insects - the part of an image which is in focus is much less than at larger scale. In subjects such as landscapes, the depth of focus may be from few metres to infinity. For subjects that are few centimetres large, the sharp area in an image is usually only a few millimetres deep (Figure 1, only top of elytra is in focus). The rest of the image is out of focus. Focus- stacking is a technique in which a number of images are taken of a subject while the camera is advanced in small increments. In the resulting series of images only a certain area in each image is in focus. The series of images can then be processed by software which combines the sharp areas of each image and creates a single image in which the entire subject is in focus. Factors which have an effect on the quality of the image are the number of images, the distance travelled between images and the accuracy with which the camera advances towards the subject. The System A single small base plate contains all components: camera and the sled, the subject and the stage and lighting. All parts are solidly mounted since unwanted movement of any component Figures 2-4: Macro photography setup. reduces the quality of the final picture. Page 2 Figure 5: Technical drawing, side view. All measurements are in mm. The system is usable for specimens when they burn out they must be from 6 mm to 80 mm length. replaced as a whole. IKEA quotes When using a macro lenses other a life of 20,000 hours which is than 105 mm focal length these probably more time than you will parameters will change. Two ever spend taking insect images. sleds were used. The camera sled moves the camera back and forth Equipment Selection in relationship to the subject. This sled must be of very high quality. Some parts were purchased on For proper stacking several images Ebay and some of these were used. are needed for each millimetre It is up to you to ensure that you that the camera travels towards are getting products that work well the subject. I found sleds from and that you buy from trustable Novoflex and Manfrotto to be suppliers. If you want guaranteed suitable for this purpose. The new and from local suppliers stage sled is used to position the you will double the price. If you specimen up/down and left/right. are worried about quality then It does not need to move very by buy the original Nikon 5100 accurately or to be of high quality power supply for $110 rather than since it does not participate in the a Chinese copy for $34 (which focus-stacking movement. It is for works just as well in my opinion). convenience since it is difficult to Not included in this setup is a PC position the subject by any other or laptop. Any computer which means (Velcro and blue tack are runs Microsoft Windows suitable unsuitable). I should mention that for the Camera Shooting software initially I bought two identical and Helicon focus versions you sleds from China to use as a stage purchase. I use the Helicon Focus sled which turned out to be badly software even though it is not free manufactured and broken. I did since I was not able to find freeware not receive a refund so this is the which performed well. Running only non-recoverable development costs are $30/year for renewal of cost ($80). The four IKEA lamps Helicon Focus software. are sufficient for lighting since slow exposure is used. They do not have replaceable bulbs and Page 3 Price Supplier Condition Item $325 Camera Sky website New Nikon 5100 $154 Ebay, seller: Photo Koeberl, Used demo Noxoflex Castel Mini Austria $54 Plastic supplier New White 20 mm thick HDPE (high-density polyethylene) $31 IKEA New 4 Jansö lights $15 Your local hardware store New Cable ties, 90 mm inside diameter drain pipe cap, magnets, epoxy $23/year Helicon Soft website New Helicon Focus software $72 Amazon www.amazon.com Used Nikon Camera Control Pro 2 $23 Bolt supplier New Stainless steel screws and bolts $18 Ebay, seller: kameradapter New EH-5A/EP-5A Power supply for Nikon 5100, Chinese copy $31 Ebay, seller: hohfashion New 4 Way Macro Focusing Rail Slider $462 Ebay Used Micro Nikkor 105mm lens Office store New A3 Vellum paper $1,208 Total The Build of vellum over the gap between the lens and the camera. Creating The skill level and tools required proper lighting is the greatest for this build are low. You must be challenge with any such setup. The able to drill precise holes by hand or base board hold all components with aid of a drill press and you will (sled/camera, specimen, lighting) have to sand plastic parts. solidly in place. HDPE is solid enough not to move but soft Technical Comments enough to drill easily. Its white color does not introduce a dark Each final image requires several reflection on the subject. dozen separate images, so the camera’s battery is drained very Correcting Tracking quickly. A permanent 240V power supply prevents this and you can I was concerned about movement then remove batteries entirely. Do of the camera sled away from an not set the f-stop (the aperture, or exact perpendicular from the internal opening of the lens) to a plane of the specimen. Previously value larger than f/11 (ie f/13, f/15 I have taken focus-stacked images …) since washout occurs. I position in which some areas, such as the lamps in such a way that they where a leg lies close to the body, do not produce much glare on an area remains fuzzy and parts of the subject (my beetles are often the specimen seem to float in the shiny and glabrous). This can mean final image. I used the following moving the light far from the vellum procedure to ensure that the ring, turning it almost parallel to the camera sled tracks perpendicular axis of view or draping an A4 sheet to the specimen: Page 4 1. Stick a size 1 insect pin in the deal with it is to increase the travel specimen mount. of the sled to include the pinhead 2. Move the camera back as far as which makes the pin sharp and possible. seem less out of place 3. Center the pin head in the image (using the camera shooting 2) Reflections. Figure 9 shows a software’s grid option and reflection of the vellum collar on zoom). the thorax. This can be reduced 4. Turn off autofocus.Focus the by moving the light further away lens on the pin head. but that also leads to a slightly 5. Move the camera sled as far underexposed area. Without forward as possible but only so spending another $100 for a high far that the pin head is still in quality head (ie Manfrotto ), in sharp focus. order to remove the camera the 6. Unless you are very lucky, the sled must be moved fully rearward pin head will have moved from so the camera attachment screw the center. The further the pin can be accessed. The adjustment of head is from the center of the the top rail of the Novoflex rail is image the worse the alignment slightly cumbersome since it is too of the camera travel is from the close to the body. A spacer would perpendicular of the specimen fix this. plane. 7. Adjust the angle between 1) the camera sled and the camera sled mount, and 2) the stage sled and the stage mount by inserting small rectangles of A4 80 g paper. 8. Check how far the pin head has moved towards the center and repeat these instructions from point 7.