DIVISION OF

Primary Faculty

William M. Maniscalco, MD Sema Hart, MD Professor of Associate Professor of Pediatrics Chief, Division of Neonatology Carl Johnston, PhD Sanjiv B. Amin, MBBS, MD, MS Research Assistant Professor of Pediatrics Associate Professor of Pediatrics Nirupama Laroia, MD Soumyaroop Bhattacharya, MEd, MS Associate Professor of Pediatrics Senior Associate of Pediatrics Medical Director, Special Care Nursery, and Section Chief, Neonatology, Rochester General Melissa Carmen, MD Assistant Professor of Pediatrics Ruth A. Lawrence, MD Director of Outreach Education Professor of Pediatrics and of and Gynecology Director, Newborn Nursery, Golisano Children’s Hospital Patricia R. Chess, MD Director, Breastfeeding and Human Lactation Study Center Professor of Pediatrics and of Director, Neonatal-Perinatal Program Alice Lewand-Taylor, MD Chair, Department of Pediatrics, Unity Hospital Senior Instructor of Pediatrics Medical Director, ECMO Service, Golisano Children’s Hospital Medical Director, Special Care Nursery, Highland Hospital Associate Chair for Education, Department of Pediatrics Thomas J. Mariani, PhD Rita Dadiz, DO Associate Professor of Pediatrics, Medicine and Environmental Associate Professor of Pediatrics Medicine Associate Director, Neonatal-Perinatal Medicine Fellowship Director, Pediatric Molecular and Program Program Director, Simulation-Based Emergency and Safety Training Jeffrey Meyers, MD Program, Division of Neonatology Assistant Professor of Pediatrics

Carl T. D’Angio, MD Robert H. Notter, MD, PhD Professor of Pediatrics and Medical Humanities Professor Emeritus of Pediatrics and of Environmental Medicine Director, Pediatric Clinical Research Office Director, Neonatal Clinical Research Michael A. O’Reilly, PhD Associate Director, SCRC Professor of Pediatrics and of Environmental Medicine Associate Director, Neonatology Research David A. Dean, PhD Director, Perinatal and Pediatric Origins of Disease Program Professor of Pediatrics and of Biomedical Engineering Dale L. Phelps, MD Fabeha Fazal, PhD Professor of Pediatrics Assistant Professor of Pediatrics Laura Price, MD Jacob N. Finkelstein, PhD Senior Instructor of Pediatrics Professor of Pediatrics, of Radiation , and of Environmental Medicine Gloria S. Pryhuber, MD Associate Director, Strong Children’s Research Center Professor of Pediatrics and of Environmental Medicine Director of Research, Division of Neonatology Arshad Rahman, PhD Ronnie Guillet, MD, PhD Associate Professor of Pediatrics and of Environmental Medicine Professor of Pediatrics Chief, Department of Pediatrics, Highland Hospital Julie Riccio, MD Assistant Professor of Pediatrics

Golisano Children’s Hospital 2013 – 2014 Annual Report Kristin Scheible, MD Robert J. Swantz, MD Assistant Professor of Pediatrics Professor of Pediatrics Director, Pediatric Sub-Internship Laurie Steiner, MD Director, Pediatric Clerkship Assistant Professor in Pediatrics Medical Director, Neonatal Transport Team, Golisano Children’s Hospital Timothy P. Stevens, MD, MPH Associate Professor of Pediatrics Jennifer L. Young, PhD Medical Director, Neonatal Intensive Care Unit, Golisano Research Assistant Professor of Pediatrics Children’s Hospital

Division Overview

The Division of Neonatology of the University of Rochester Medical Center is dedicated to excellence in patient care, to diligent research and to providing outstanding education and training of and scientists. Our values dictate that all colleagues, trainees, patients, and families are treated with respect. The major clinical site is the Neonatal Intensive Care Unit (NICU) at Golisano Children’s Hospital. Our NICU was selected as one of the top NICUs in the country by US News and World Report in 2012. The Special Care Nursery (SCN) at Rochester General Hospital (RGH) is integral to our patient care mission. Our basic research focuses on the molecular and cellular regulation of lung development and injury. Patient-oriented research spans important neonatal diseases, with a focus on treatment of lung, neurological, and retinal disorders. Our faculty members provide clinical education and training to medical students, residents, postdoctoral fellows, nurse practitioners, and community practitioners. An important part of our mission is training in basic and clinical research at many levels, ranging from undergraduates to doctorates in medicine and the basic sciences.

Clinical Activities

Golisano Children’s Hospital Our clinical goal is to provide outstanding care to all ill newborns in the Finger Lakes Region. The neonatal intensive care unit (NICU) at Golisano Children’s Hospital (GCH) is a level IV facility that can treat all neonatal medical and surgical illnesses. Our 60-bed NICU has extensive technical capabilities, including ECMO, inhaled nitric oxide, and HFOV. Construction is nearly complete on our new children’s hospital that will have an all new NICU, expanding our capacity with 64 individual patient rooms. In 2013 – 2014, our 15 board-certified neonatologists treated 1291 newborns in the NICU; these patients had the full range of medical, surgical, and cardiac disorders. The average daily census in the NICU in 2013– 2014 was 60.7 patients, for a total of 22,155 patient days. Of the 3020 patients born at Strong Memorial Hospital, 1041 were admitted to the NICU. An additional 250 patients who were born at either community or at other regional hospitals were transferred to our NICU for advanced care. Our neonatal transport service transferred patients from 19 referring hospitals. Approximately 1 in 12 newborns in our region was treated in the NICU at Golisano Children’s Hospital. In 2013 – 2014, 84 patients were transferred back to their community hospital for convalescent care and to be closer to their families.

Our very busy NICU is the site for training Pediatric and -Pediatric house officers, postdoctoral fellows, medical students, neonatal nurse practitioners and assistant students. The Neonatology Division also supervises the Newborn Nursery (NBN) at Strong Memorial Hospital. Two board-certified pediatricians establish policy and procedures and care for newborns under routine care.

Rochester General Hospital, Unity Hospital, Highland Hospital At RGH, in 2013 – 2014 the Neonatology Division saw 2470 births and cared for 417 patients in the Level II Special Care Nursery (SCN), yielding an average daily census of 6.6. As a community hospital, the SCN is an important training site for Pediatric and residents and medical students. Members of the Neonatology Division serve as medical directors for the nurseries at Rochester General, Highland, and Unity Hospitals, and are responsible for establishing consistent policies and procedures for local hospitals.

Golisano Children’s Hospital 2013 – 2014 Annual Report Neonatal Continuing Care Program To provide outstanding medical care, it is essential that we follow the neurodevelopmental outcome of our NICU graduates. The Neonatal Continuing Care Program follows all patients who are discharged from the NICU, either in the and Toddler (part of the Strong Center for Developmental Disabilities) or in the Neonatal Tracking Program. In the Infant and Toddler Clinic, patients are evaluated by a developmental pediatrician, social worker, a and a neonatology fellow; in 2013 – 2014, the clinic conducted 363 evaluations. The Infant and Toddlers Clinic also evaluates patients who were not in the NICU, but referred from the Monroe County Early Intervention Program. The Neonatal Tracking Program follows all NICU graduates to age 10 through serial questionnaires sent to the patient’s family and pediatrician. The NCCC teams review the tracking forms to identify patients who may need formal evaluation and to obtain long-term follow-up data. In 2013-2014, 1410 forms were returned by parents and 2764 forms were returned by pediatricians. To date, over 26,000 patients have been followed by the tracking program.

NICU Quality Improvement Programs / Initiatives In 2013 – 2014, the NICU initiated several New York State (NYS)-wide and locally developed quality improvement initiatives:

NYS Perinatal Quality Collaborative: The NICU worked with the NYS Perinatal Quality Collaborative to improve growth outcomes of patients < 31 weeks gestation and to reduce the rate of Central Line Associated Bloodstream (CLABSIs).

o Growth: the proportion of < 31 weeks gestation discharged < 10th percentile has decreased. This has been accomplished through use of trophic feedings, earlier introduction of feedings (median age at first feeding decreased from 4 days in 2011 to 3 days in 2012), earlier advancement of protein and lipid in TPN, and routine nutrition rounds during which growth of current inpatients is reviewed. o CLABSI: after decreasing for several years, the CLABSI rate plateaued in 2012. To generate improvement going forward, the NICU has implemented routine use of central catheter insertion and catheter maintenance checklists. Compliance with checklist use will be quantified and reported to NYS. In addition, we have conducted a NICU-wide educational program on care and maintenance of central catheters.

NYS Medical Home Grant: As part of a NYS Medical Home Grant awarded to SMH, the NICU has focused on reducing nosocomial infections (NI) caused by either primary blood stream infections (BSIs) (primary BSI, including CLABSIs and BSIs without a source) and secondary BSIs (BSIs attributed to a specific source). Specific efforts have included:

o Alcohol caps on all hep locks. o Proper collection of blood cultures to reduce contamination. Locally Initiated Quality Improvement (QI) Projects:

o Reducing incidence of severe ROP: a re-education effort was initiated to emphasize appropriate saturation targets. o Educating parents on safe sleep practices: In 2013-14, the NICU and NBN implemented mandatory education in safe sleep practices for parents of all newborns. This initiative was developed in response to an increase in sudden infant death noted by the SMH Pediatric ED staff. o IVH Reduction Bundle: More judicious use of volume expansion in treating neonatal hypotension was reinforced as was reducing use of sodium bicarbonate. o Improving the proportion of infants receiving breast milk at days of life 14 and 28 as well as at NICU discharge.

Regional Outreach Activities The Neonatology Division is the Regional Perinatal Center for the Finger Lakes Region of New York State. Integral to the Division’s clinical activities are outreach visits to the 11 referring hospitals in our region. The goals of these visits are to review maternal and neonatal outcomes at the referring hospitals, provide feedback on patients transferred to the NICU at GCH, review clinical policies and procedures, and provide outreach education. In addition, the outreach team compares outcome statistics from the referring hospital to region-wide data. Each hospital is visited one to two times a year by a

Golisano Children’s Hospital 2013 – 2014 Annual Report neonatologist, high-risk perinatologist, neonatal nurse practitioner, and obstetrical nurse practitioner. Melissa Carmen, MD, is responsible for the majority of outreach teaching visits.

Outreach Hospitals: • Arnot Ogden Medical Center, Elmira, NY • Unity Hospital, Rochester, NY • Corning Hospital, Corning, NY • Olean General Hospital, Olean, NY • F.F. Thompson Health System, Canandaigua, NY • Rochester General Hospital, Rochester, NY • Highland Hospital, Rochester, NY • St. James Mercy Hospital, Hornell, NY • Jones Memorial Hospital, Wellsville, NY • Via Health of Wayne, Newark, NY • Memorial Hospital, Dansville, NY

Research and Other Scholarly Activities Research Projects by Faculty Members

Sanjiv B. Amin, MBBS, MD, MS chest compressions during neonatal resuscitation. She continues Associate Professor, Pediatrics to collaborate with the University of Buffalo to study this area of resuscitation in a newborn piglet model. Dr. Amin’s research interests include the effects of jaundice, nutritional, and environmental toxins on the developing nervous system in neonates using auditory brainstem responses. He has NIH funding to evaluate dental developmental outcome in Patricia R. Chess, MD premature infants and to study the level of jaundice associated Professor, Pediatrics with transient or permanent abnormal changes in auditory nervous system in premature and term neonates. In addition to Dr. Chess’s research is on lung injury and repair in the neonate, assessing the antioxidant effect of jaundice on neonatal focusing on mechanical forces in the lung. Collaborative work on morbidities, he investigates language disorders and central assessing efficacy of a biophysically active phospholipase auditory processing disorders of premature and late preterm resistant synthetic surfactant in an LPS-induced injury murine neonates. Dr. Amin is interested in genetic polymorphism and model compliments this work. Clinical areas of investigation ethnic differences in bilirubin production and susceptibility to include optimal modes of ventilation, use of ECMO in bilirubin-induced neurotoxicity. He explored a new technique respiratory failure, cardiorespiratory dysfunction in congenital using fluorescent spectrophotometry to measure free bilirubin diaphragmatic hernia, omega-3 lipids to treat TPN-induced liver levels, a more specific marker of bilirubin-induced neurotoxicity. dysfunction, and surfactant replacement . A pulmonary He studies the effect of lead and other environmental toxins on a potentially best practices bundle for VLBW infants in the NICU developing auditory nervous system, and the role of nutritional has been developed and implemented, and its effect on acute and (iron and Vitamin D), inflammatory, and hormonal factors on chronic lung disease is also being investigated. A respiratory brain development. He has two randomized trials; the first monitor to assess chest movement in babies on high frequency evaluates the role of lipid administration in the development of ventilation is being developed in collaboration with Biomedical cholestasis in neonates, and the second evaluates the association Engineering at UR. Dr. Chess is the site PI for the NICHD between iron deficiency and brain myelination. His work is neonatal network randomized controlled inositol trial to assess supported by NICHD, CTIS, and NIDCR. efficacy of inositol supplementation on improving ROP and BPD and efficacy of hydrocortisone to facilitate extubation in VLBW infants. Dr. Chess participates in grants from NICHD and NIHLB. Melissa Carmen, MD Assistant Professor, Pediatrics

Dr. Carmen is involved in various clinical trials through the Rita Dadiz, DO NICHD Neonatal Research Network. She is Co-PI in the Associate Professor, Pediatrics Transfusion of Prematures (TOP) study, which aims to determine if maintenance of higher hemoglobin levels in extremely low Dr. Dadiz incorporates simulation-based learning and debriefing birth weight babies leads to improvement in the survival and methodologies into postgraduate medical and education rates of neurodevelopmental impairment at 22-26 months. Dr. with the goal of improving interprofessional communication and Carmen is also site Co-PI for the INS-3 trial, a randomized teamwork during patient care. With validated evaluation tools, controlled trial to assess the efficacy of inositol supplementation she is investigating the impact of simulation-based team training on improving ROP and BPD. In 2012, while still in training at for obstetric and neonatal providers on communication and the University of Buffalo, Dr. Carmen was awarded a Young patient outcomes during high-risk deliveries. Dr. Dadiz is the Investigators Award from the Neonatal Resuscitation Program to recipient of an award from the Health Resources and Services study the use of pulse oximetry in the assessment of effective Administration to train healthcare providers throughout the

Golisano Children’s Hospital 2013 – 2014 Annual Report Finger Lakes Region of New York State on the use of nucleus and how this relates to their ability to be expressed. simulation-based learning and debriefing in quality improvement Taken together, these studies will help design improved vectors initiatives. Dr. Dadiz is also the site director for the International for gene therapy as well as provide insight into the basic biology Network for Simulation-Based Pediatric Innovation, Research of the cell. The second focus of the laboratory is to develop novel and Education (INSPIRE). The INSPIRE network is currently treatments for acute lung injury and asthma using electric fields evaluating competency-based infant lumbar puncture training on for gene delivery to the lungs of living animals. Dr. Dean’s interns’ lumbar puncture success rates. Dr. Dadiz is collaborating laboratory has shown that genes can be delivered to the lungs of with Dr. Pasternack from the URMC Emergency Department to small and large animals by electroporation and that genes can lead a qualitative study for the INSPIRE network examining the have significant therapeutic activity. Using this approach, they factors that facilitate and impede successful implementation of have been able to prevent and treat acute lung injury in an the lumbar puncture competency-based assessment program. endotoxin-induced mouse model of lung injury as airway hyperreactivity in an ovalbumin-sensitization model of asthma. Current studies assess safety and efficacy in a large animal preclinical model in hopes of one day taking this to the clinic. Dr. Carl T. D’Angio, MD Dean’s research is supported by several NIH RO1 and R21 Professor, Pediatrics grants.

Dr. D’Angio is the principal investigator for the joint University of Rochester – University of Buffalo center of the NICHD Neonatal Research Network (NRN); which performs clinical Fabeha Fazal, PhD trials and observational studies on critically ill newborns cared Assistant Professor, Pediatrics for in a consortium of 18 neonatal centers. The NRN currently seeks treatments for respiratory diseases, neonatal Dr. Fazal’s laboratory investigates the role and regulation of encephalopathy and necrotizing enterocolitis, among other cytoskeletal dynamics in endothelial cell (EC) inflammation, disorders. Dr. D’Angio serves as the URMC site investigator for permeability, and apoptosis in the settings of acute and chronic the Prematurity and Respiratory Outcomes Program (PROP), an lung diseases. A key event underlying the pathogenesis of acute NIH-funded longitudinal study of the antecedents of respiratory lung injury involves adhesion molecule-dependent disease in the first year among premature infants. He is an transendothelial migration of polymorphonuclear leukocytes investigator at UR’s new NIAID-funded Respiratory Pathogens (PMN) into the lungs. Transcription factor NF-kB plays a critical Research Center, which will explore respiratory infections role in the expression of adhesion molecules ICAM-1 and among premature infants. VCAM-1. Activation of NF-kB requires its release from the inhibitory protein IkBa in the cytoplasm and subsequently, its Dr. D’Angio studies infections, and immunizations translocation to the nucleus. Whereas the mechanisms of its in the premature infant. He is concerned with comprehension of release have been elucidated, the cytoplasmic events regulating research permission among parents of infant research subjects, the translocation of NF-kB to the nucleus remain elusive. Dr. for which he completed an NICHD-funded randomized Fazal and colleague have uncovered novel and previously controlled trial of a cover sheet to improve understanding of unrecognized role of actin cytoskeleton and non-muscle myosin research consent. light chain kinase (nmMLCK) in the regulation of cytoplasmic trafficking of NF-kB for its nuclear import to cause EC inflammation associated with intravascular coagulation and . David A. Dean, PhD Professor, Pediatrics More recently Dr. Fazal’s research has focused on identifying the role of endoplasmic reticulum (ER) stress and mitochondrial Dr. Dean’s laboratory is focused on the mechanisms of dysfunction in the regulation of EC inflammation and intracellular trafficking of plasmids and DNA-protein complexes permeability associated with ALI. Their findings show that ER as it relates to nonviral gene delivery to the lung. While many stress regulator BiP and mitochondrial stress regulator Mortalin aspects of non-viral vector design are being addressed, critical are mediators of EC inflammation and they may act in concert to areas that have not received adequate attention are the regulate cytosolic and nuclear events to promote NF-kB activity. cytoplasmic movement and nuclear import of vector DNA. In addition, Dr. Fazal and Dr. Rahman collaborate to understand Clearly, without the translocation of plasmid DNA into the the role of nmMLCK in the pathogenesis of pulmonary nucleus, no gene expression, or gene therapy can take place. To emphysema caused by cigarette smoke (CS). They utilize this end, they study how plasmids interact with microtubules and genetically modified mice, primary cell cultures, and microtubule-associated motor proteins to move in a directed multidisciplinary approaches ranging from biochemical, cellular, fashion through the cytoplasm using a variety of intracellular and molecular biology to in vivo gene delivery and lung imaging techniques, biochemical assays, and mass spectrometry physiology to study the response of pulmonary endothelium approaches. Studies are also underway to understand how during acute and chronic lung inflammation/injury. plasmids translocate the nuclear pore complex to gain entry into the nucleus in both general and cell-specific situations. New work examines whether and how plasmids move within the

Golisano Children’s Hospital 2013 – 2014 Annual Report Jacob N. Finkelstein, PhD studies of acute kidney injury in premature infants. Her work is Professor, Pediatrics supported in part by grants from the NIH and FDA.

Dr. Finkelstein’s laboratory investigates the role of cell-cell interactions in modulating the pulmonary injury response to physiological and toxicological stimuli. This work includes Carl Johnston, PhD studies of oxidant-induced signaling in the pulmonary epithelium Research Assistant Professor, Pediatrics and macrophages and epithelial and inflammatory cell production of cytokines and chemokines in the regulation of the Dr. Johnston explores the postnatal lung copes with external inflammatory response. Prior research focused on basic cell and stress. A critical biological factor playing a role in childhood molecular biology of the pulmonary alveolar type II cell as the pulmonary susceptibility is the extent of lung development site of pulmonary surfactant system and how oxidant injury can occurring postnatally. One of the environmental factors relevant alter key regulatory processes. The type II cell plays an important to developmental lung disease is the recent increase in role as the stem cell for renewal of the alveolar epithelium, both complexity and distribution, if not the levels, of airborne in the normal lung development and during epithelial repair and pollutants including allergens and endotoxins, respirable renewal following lung injury. The most recent data suggest that particulate matter, and irritant gases, exposure to which damages type II cells may be involved in regulating the inflammatory various cell types. Among the most important of these are the functions of alveolar macrophages, as well as the actions of respiratory epithelium and critical immune effector cell interstitial fibroblasts during lung growth or pulmonary fibrosis. populations. Combined exposures to multiple pollutants may activate several unique signaling pathways that are age Current research utilizes multiple models of injury with a special dependent and, depending on the sequence of initiation, may emphasis on environmental agents as modulators of cellular result in responses not predicted by evaluating exposures to an function. Studies of inhaled oxidant gases (ozone, hyperoxia), individual pollutant. Dr. Johnston’s work relies on mRNA inhaled particulate matter (generated by combustion processes analysis, microarray technology, immuno-histochemistry, and in including diesel exhaust) and ionizing radiation (therapeutic as situ hybridization. This work is supported by participation in well as accidental release) all provide a platform to study the several NIH grants. pulmonary injury response. Another important aspect of recent work is the interaction of engineered nonmaterials with cellular signaling systems and how these interactions may result in a toxic response. Nirupama Laroia, MD Associate Professor, Pediatrics The overall goal of the current research is to identify the key control mechanisms involved in epithelial cell and fibroblast Dr. Laroia focuses on neonatal seizures, especially those related proliferation, and in related extracellular matrix synthesis, to stroke and hypoxic ischemic injury in the newborn. She has processing, and assembly. Dr. Finkelstein seeks to define such developed criteria for long-term EEG monitoring in neonates at mechanisms not only during normal lung growth and normal risk for seizures. Current projects include study of development, but also during the repair of epithelial damage, neuroprotective strategies in infants with hypoxic ischemic which may have important implications for pediatric and adult injury, Hyperbilirubinemia in the term neonate, use of donor lung disease, including bronchopulmonary dysplasia (BPD). This milk in preterm infants, and a qualitative study looking at work is funded by grants from the NIEHS, the EPA, NIAID, barriers to communication between obstetricians and NSF and the DOD. pediatricians in the delivery room. Her research interests include neonatal global health with implementation of Helping Babies Breathe program in India.

Ronnie Guillet, MD, PhD Professor, Pediatrics Ruth A. Lawrence, MD Dr. Guillet’s primary research focus is neonatal brain Professor, Pediatrics development and injury. The developing brain is at risk from the effects of centrally acting agents, including hypoxia, seizures, Dr. Lawrence is a consultant to and an investigator with Dr. Ann and medications; this risk is due in part to the fact that the brain Dozier in the Department of Sciences. She is the may be more vulnerable during times of rapid maturational medical director for the Lactation Study Center, a drug change. Dr. Guillet has an active clinical research program information line, and the clinical toxicology service. Current focused on the neonatal brain. As the Rochester co-investigator projects include the impact of epidural on for the NICHD Neonatal Research Network grant, she assists the breastfeeding; the impact of Depoprovera given to the mother PI and is responsible for network studies involving new before discharge on breastfeeding. treatments for neonatal brain injury. Other areas of clinical research interest include the auditory brainstem evoked response, Dr. Lawrence has been involved in efforts to provide human milk its use as a tool to evaluate brain maturation, and its relationship for prematures. She was elected to the board of the newly to maternal iron status prior to delivery. She is also involved in established Mothers Milk Cooperative. Donors are screened by

Golisano Children’s Hospital 2013 – 2014 Annual Report the American Red Cross. The process is approved by the FDA. NIH.

Dr. Lawrence collaborates with Dr. OJ Sahler on the effect of music on breast milk production. Music therapy may increase breastfeeding rates among mothers of premature infants not only Jeffrey Meyers, MD in the first few days but also at 60 days post-delivery, most likely Assistant Professor, Pediatrics because of its relaxing effects. When a mother is having difficulty breast feeding, she is usually given instructions about Dr. Meyers primarily examines neonatal nutrition. The ways to relax, including deep breathing, visualization exercises, independent role of early nutrition in influencing long-term or listening to music. A study of mothers listening to music health is becoming better appreciated, particularly in preterm during kangaroo care showed that music not only significantly infants. In addition, body tissue accrual in preterm infants is decreased maternal anxiety, but also increased quiet sleep and altered at term-equivalent age. His previous research has reduced crying in the infant. There is a growing body of literature investigated in-hospital nutritional factors that might affect body to support the notion that the benefit of live music is superior to composition in preterm infants. He demonstrated the potential recorded music in inducing relaxation in infants although there influence of parenteral nutrition on body composition at are few studies in mothers. This is a particularly important discharge in preterm infants. Currently, Dr. Meyers serves as a finding given the evidence that maternal anxiety/stress as site PI for the MILK Trial, an NRN-funded study comparing reflected in increased cortisol levels in breast milk can negatively neurodevelopmental outcomes in preterm infants fed primarily affect infant temperament. donor breast milk versus formula. Future direction includes investigating other influences, such as levels of pro-inflammatory cytokines, on altered body composition in preterm infants, and possible avenues to optimize tissue accrual in these at-risk William M. Maniscalco, MD infants. Long-term metabolic consequences of altered body Professor, Pediatrics composition in preterm infants also warrant further study in the era of the metabolic syndrome. Dr. Maniscalco studies pulmonary microvascular development in lung injury. Using various animal models, including a non- human primate model of BPD, this work examines the effects of oxygen and ventilation of immature lung on the development of Robert H. Notter, MD, PhD alveolar capillaries. The major goals of the research are to Professor Emeritus, Pediatrics characterize microvascular development in lung injury and investigate angiogenic and angiostatic regulators in normal and Dr. Notter’s research focuses on pulmonary surfactant, a injured lung. Recent work has linked expression of inflammatory complex mixture of lipids and proteins essential for normal CXC chemokine mediators, which regulate angiogenesis and are respiration. Lung surfactant deficiency in premature infants leads part of the pathophysiology of BPD, to impaired lung to the neonatal respiratory distress syndrome (NRDS), and microangiogenesis. surfactant dysfunction/inactivation in patients of all ages is an important contributor to the of clinical acute lung injury (ALI) and the acute respiratory distress syndrome (ARDS). The severe lung injury syndromes of ALI/ARDS can Thomas J. Mariani, PhD result from multiple direct pulmonary insults and are major Associate Professor, Pediatrics causes of mortality and morbidity in the US and worldwide despite sophisticated medical intensive care. This multi- The broad objective of Dr. Mariani's research is the identification university collaborative research emphasizes basic science and of the genetic mechanisms of susceptibility to chronic lung translational studies to develop and test new highly-active diseases, particularly focusing upon their developmental synthetic lipid/peptide exogenous surfactants for treating NRDS antecedents and the influence of environmental factors. They and direct pulmonary forms of ALI/ARDS. Current NIH seek to define key regulatory networks involved in lung Bioengineering Research Partnership (BRP) studies in our development and maturation, and which may be perturbed in laboratory examine the molecular bioengineering, computer diseased states such as asthma, chronic obstructive pulmonary analysis, synthesis, and physical chemistry of novel amphipathic disease (COPD) and bronchopulmonary dysplasia (BPD). More peptides with structural homology to native surfactant proteins recent studies focus upon factors contributing to severity of (SP), and the surface and pre-clinical pulmonary activity of these pathogen-driven respiratory disease. Work focuses peptides combined with novel phospholipase-resistant lipids or predominantly upon defining pathways contributing to regulation synthetic biologic lipids in synthetic lung surfactants. of epithelial-mesenchymal interactions and expression of extracellular matrix molecules. His laboratory utilizes genetic Dr. Notter’s research also examines mechanisms of surfactant modeling in animals, exposure-related models of chronic lung dysfunction in ALI/ARDS, and considers the use of exogenous disease and studies of human samples. He is a leader in the surfactants in combination with other pharmaceuticals to application of genome-wide expression array methods to concurrently target multiple aspects of lung injury pathology. pulmonary biology and lung disease gene/biomarker discovery. Prior research helped to develop clinically-effective first- This work is currently supported by research grants from the generation surfactant therapy for premature infants with animal-

Golisano Children’s Hospital 2013 – 2014 Annual Report derived surfactant drugs, and this is now being extended using Gloria S. Pryhuber, MD sophisticated molecular bioengineering to define and develop Professor, Pediatrics novel fully-synthetic lipid/peptide lung surfactants for treating NRDS and direct pulmonary ALI/ARDS. As a clinician scientist, Dr. Pryhuber maintains a basic science laboratory and serves as communicating PI for the University of Rochester/University at Buffalo (URUB) research site for the Prematurity and Respiratory Outcomes Program (PROP, NHLBI Michael A. O’Reilly, PhD U01). Six Pediatric Pulmonary/Neonatology centers around the Professor, Pediatrics country comprise PROP, which identifies biomarkers and mechanisms of chronic lung disease in premature infants. In the The O’Reilly lab is an interactive group of senior scientists, development and now implementation of PROP, she works very graduate students, technicians, and summer undergraduate closely with the Rochester Human Immunology Center to bring scholars interested in understanding how neonatal oxygen advances in immunologic techniques to the study of premature disrupts lung development and the host response to respiratory newborns. Because of her training as a neonatologist and the viral infections. Premature exposure to oxygen is a major risk strong infrastructure of PROP and the NICHD Neonatal factor for neonatal lung disease and can cause bronchopulmonary Research Network, including collaborative arrangements with dysplasia (BPD), a chronic form of lung disease frequently seen the Maternal Fetal Medicine, Neonatology and Pediatric in preterm infants with very . While better Pulmonary Divisions, Dr. Pryhuber has adequate numbers of clinical care has reduced mortality, children born prematurely premature and full term infant patients, ensuring the timely remain at increased risk for reduced lung function, respiratory screening, enrollment, and completion of clinical studies/trials viral infections, pulmonary hypertension, and asthma. According with these populations. Her laboratory has notable experience in to the NHLBI, the annual cost of treating infants with BPD in collecting, storing, processing, and performing quality 2005 was $26.2 billion dollars. Hence, there is an urgent need to assessment and analysis of animal and human subject samples. understand how oxygen supplementation permanently disrupts lung development and how these changes enhance susceptibility She works with a number of investigators at UR and external on to respiratory infections. Using the mouse as a model system, we Identification and Validation of Molecular Markers for BPD, to seek to define how high oxygen at birth alters growth and investigate and validate altered gene expression in human differentiation of progenitor cells critical for lung development neonatal lung samples with and without chronic lung disease. and host defense against infections. We also investigate how She collaborates with the Human Immunology Center, and the oxygen-induced damage activates molecular pathways that Divisions of Neonatology, Pediatric Infectious Diseases, control whether cells live or die, collaborating with physicians Pediatric , and Medicine Infectious Diseases. She who study health outcomes and treat children born prematurely. has collaborated with Drs. Topham, Gill, and Caserta on two By integrating research findings in cells, mice, and humans, we NIH proposals to study CD8+ T cells in responses to viruses and hope to ultimately identify therapeutic opportunities for vaccines with correlations to gut microbiomics and measures of improving the long-term health of children born prematurely. immune modulators in breast milk. She continues these This work is supported by several NIH grants. collaborations for the National Respiratory Pathogens Research Center (RPRC, PIs David Topham and Ann Falsey). With Dr. Caserta, she has initiated a project entitled Impact of Respiratory Virus Infections and Bacterial Microbiome Shifts on Lymphocyte Dale L. Phelps, MD and Respiratory Function in Infants Born Prematurely or Full Professor, Pediatrics Term. Dr. Pryhuber is highly enthusiastic to work with this multidisciplinary group of collaborators and the promise of Dr. Phelps focuses on retinopathy of prematurity (ROP). Her contribution to patient-specific tailoring of treatment and NIH funded work has completed two pilot studies on the prophylaxis for respiratory morbidity in infants. pharmacokinetics of inositol and its safety in extremely preterm infants, supplemented from birth to reduce ROP, and provided the necessary background to support a large multicenter randomized controlled trial funded by both the National Institute Arshad Rahman, PhD of Child Health and Human Development (NICHD) and the Associate Professor, Pediatrics National Eye Institute. This multicenter research, which is FDA approved and NIH funded, began enrollment this year and targets Dr. Rahman and his group want to know how pro-inflammatory three to four years to reach full enrollment (n=1760). mediators such as the coagulation protease thrombin and the pro- inflammatory cytokine TNFα, released in high amounts during sepsis and other inflammatory conditions, mediate neutrophil (PMN) sequestration and emigration in the lung and thus induce lung vascular injury. They seek definition of the critical signaling pathways mediating activation of the transcription factor NF-κB, a master regulator of inflammation, in the endothelium, an important cellular target of proinflammatory mediators in perpetuating and amplifying the inflammatory responses. NF-κB

Golisano Children’s Hospital 2013 – 2014 Annual Report is now also implicated in the resolution phase of inflammation, mediated in part by dysregulation of immune cells, including tissue repair and homeostasis, and in mitochondrial respiration. BPD, PVL and NEC. The selective susceptibility to viruses and These emerging new roles of NF-κB have challenged the view of inflammation-mediated diseases suggests a defect in CD8+ T cell therapeutic inhibition of NF-κB and have yielded the concept responses compromising their antigen recognition and immune that dampening rather than abolishing NF-κB activation may be a regulation. Little is known about the nature of CD8+ T cell safe and effective treatment strategy for ALI/ARDS and other responses in neonates, and less is known about CD8+ T cell inflammation-associated diseases. Guided by this principle, Dr. behavior and function in premature infants. Her research focuses Rahman and colleague focus on uncovering the intricate on the effect of early activation of CD8+ T cells in the context of signaling network in control of NF-κB activation with a view to premature infants with poor thymic recovery. Employing identifying the possible therapeutic targets whose inhibition may multiplexing technology such as high-parameter cytometry to allow for selective suppression of detrimental inflammation analyze human umbilical and peripheral cells, she is able to without compromising the host defense response, tissue repair examine T cell phenotype, homeostasis and antigen-specific and homeostasis – a problem associated with current treatment responses, as well as trace changes that occur over time in options to control ALI/ARDS and other inflammatory disease postnatal immune development. By combining in vitro data with states. clinical data, she can understand the role that T cell dysregulation may play in mediating diseases of prematurity. Her work is Dr. Rahman and colleague study the regulation and function of supported by an NIH K12 award. endothelial cell (EC) autophagy and its relationship with EC inflammation in the context of ALI. Their novel and exciting findings that autophagy and inflammation are linked in the lung endothelium have prompted them to identify the mechanistic link Laurie Steiner, MD between these two fundamental processes (autophagy and Assistant Professor, Pediatrics inflammation), address the relevance of this linkage in the pathogenesis of ALI, and evaluate the therapeutic potential of Red blood cells comprise one in four cells in the . To autophagy inhibition against evolving ALI. The lab uses maintain an appropriate hematocrit, the body must produce genetically modified mice, primary cell cultures, and approximately 2 million red blood cells per second. The multidisciplinary approaches ranging from biochemical, cellular, maturation of a committed erythroid progenitor to a functional and molecular biology to in vivo gene delivery and lung red blood cell is a complex process that involves significant physiology to address these questions of fundamental changes in gene expression during a time of rapid cell division. importance. Dr. Rahman’s work is supported by grants from The goal of Dr. Steiner’s research is to understand the molecular NIH. mechanisms that drive this process, in both normal and disease states. She is particularly interested in studying how chromatin modifiers regulate erythropoiesis, as they are potential therapeutic targets for the treatment of hemoglobinopathies, such Julie Riccio, MD as sickle cell and betathalassemia. By combining Assistant Professor, Pediatrics genomics technologies, such as chromatin immunoprecipitation coupled with high throughput sequencing, with functional assays, Dr. Riccio focuses on in premature such as shRNA knockdown, she is able to study how chromatin neonates. Her prior work assessed the use of Ibuprofen versus modifiers regulate erythroid-specific gene expression and indomethacin for medical treatment of PDA among level 3 influence erythroid maturation. Complimentary to this project, NICU’s across the United States. She also studied the effect of she uses similar methodologies to study the cis- and trans- acting Ibuprofen, used for closure of the PDA, on bilirubin-albumin factors that regulate modifier genes of betathalassemia, with the binding. hope of identifying new targets for therapeutic intervention. This area of research will enhance our understanding of how She is now piloting a study with a subset of NRN sites, headed ubiquitously expressed chromatin modifiers exert cell-type by Children’s Hospital of Philadelphia. This pilot reviews the specific effects, as well as provide novel insights into molecular incidence and natural history of vocal cord injury after PDA pathways important in human disease. Her work is supported by ligation in premature infants. Dr. Riccio also serves as a sub- a NIH K08 award, the Cooley’s Anemia Foundation, and the investigator with a study, Noninvasive Optical Brain Imaging of American Society for (ASH). Infants Born Prematurely, for which Lauren Emberson, PhD, and Richard Aslin, PhD, of UR’s Department of Brain and Cognitive Sciences, manage follow up activities after discharge. Timothy P. Stevens, MD, MPH Associate Professor, Pediatrics

Kristin Scheible, MD Dr. Stevens researches pulmonary outcome of premature infants, Assistant Professor, Pediatrics with emphasis on the neonatal antecedents that cause preterm infants to require ongoing pulmonary care in childhood. Studies Premature infants are highly susceptible to severe disease from include two prospective clinical research projects investigating respiratory viral , and they suffer from diseases the effect of supplemental oxygen exposure on the risk of

Golisano Children’s Hospital 2013 – 2014 Annual Report wheezing in later childhood. As a secondary study to the NICHD Jennifer L. Young, PhD SUPPORT Trial, a randomized trial of clinical treatment of high Research Assistant Professor, Pediatrics – vs. low – targeted oxygen saturations in preterm infants < 28 weeks’ gestation, Dr. Stevens investigates the effect of Dr. Young investigates extracellular matrix (ECM) signaling differential oxygen exposure on symptomatic airway dysfunction during lung injury and repair, specifically the role of the in preterm infants. In a Rochester-based cohort, the level of early matricellular signaling molecule CCN1/Cyr61 in lung injury. oxygen exposure and consequent oxidant stress is investigated as CCN1 is an ECM-associated signaling molecule that functions to predictors of later symptomatic airway dysfunction in premature promote cell adhesion, migration, survival and differentiation in infants 28-32 weeks’ gestation. Dr. Stevens is also interested in vascular development. In the context of the lung, they found that the effects of air pollution on and pulmonary CCN1 together with TNFα causes apoptosis of alveolar epithelial symptoms in preterm infants. cells and lung fibroblasts. Because CCN1 is induced in the lungs of patients with lung diseases such as chronic obstructive pulmonary disease (COPD) and acute respiratory distress syndrome (ARDS), they hypothesize that CCN1, together with Robert J. Swantz, MD inflammatory mediators such as TNFα, causes cell death of Professor, Pediatrics primary lung cells in vivo, thus contributing to lung injury. The studies focus on the molecular mechanism(s) of cell death and Dr. Swantz' primary research interest is undergraduate medical survival of primary lung cells in response to CCN1 and TNFα rd th education. He directs the 3 year Clerkship and 4 year Sub- using cultured cells as well as in vivo models of lung injury. Internship for the Department of Pediatrics, and is actively involved with the Dean's Office in the management and In addition to CCN1’s apoptotic function, Dr. Young’s team evaluation of the curriculum and the admission found that CCN1 functional knock-out mice have greatly reduced and advancement of medical students. lung inflammation after LPS treatment, suggesting that CCN1, in addition to playing a role in apoptosis and cell survival, is likely to be involved in the inflammatory response as well, leading them to investigate the modulation of inflammation by CCN1 in models of acute lung injury and fibrosis in vivo. The ultimate goal is better understand the complex pathology of lung diseases to improve treatments. Research funding comes from the American Heart Association.

Scientific Presentations

Abstracts Presented at 2014 Pediatric Academic Societies’ Annual Meeting, Vancouver, BC Canada

Amin SB, Wang H. Unbound Bilirubin is a Amin SB, Orlando M, Monczynski C, Tillery Pasternack JR, Dadiz R, McBeth R, et al Better Predictor than Total Serum Bilirubin of K. Auditory Processing Disorder in Children Barriers of a competency educational model Jaundice Associated Apnea in Premature Born Prematurely: Auditory Profile. for infant lumbar punctures. Infants. Amin SB, Vogler D, Wang H. Predischarge Parika P, Alfieris G, Swantz M, Dean D. Amin SB, Gupta K, Rallabandi S, Wang H. Auditory Neural Myelination Predicts Platform Session: Neonatal Postnatal Iron Status is Associated with Language Outcome at 3 Years Corrected Age Weight Gain During Early Infancy in in Premature Infants. Parikh P. Alfieris G, Swartz M, Dean D. Premature Infants. Platform Session: Neonatology: Cardiology 1

Golisano Children’s Hospital 2013 – 2014 Annual Report Abstracts Presented at 2014 American Thoracic Society 110th International Conference, San Diego

Bhattacharya S, Misra R, Huyck H, Mariani T, O’Reilly M. Next generation Pryhuber G. Subpopulation Dissociation, Claunwhite C, Chu C, Lunger V, Lopez A, sequence analysis of the transcriptional Recovery and Characterization of Lung Cells Ashton J, Castiglione S, Maffett D, Werner E, response to neonatal hyperoxia. from Human Pediatric Lungs Using Flow Scalise T, Horan A, Sacilowski M, Misra S, Cytometric and Molecular Techniques. Gill, Ryan R, Reynolds A, Pryhuber G, Chu C, Qiu X, Bhattacharya S, Slaunwhite C, Mariani T. Defining the effects of Prematurity Lunger V, Lopez A, Myers J, Holden-Wiltse J, Solleti S, Lunger V, Lopez A, Srisuma S, on the Lymphocyte Transcriptome. Gill S, Wu H, Treanor J, Mariani T, Topham Rangasamy T, Mariani T. Serpin E2 D. Characterizing the Early Transcriptomic Deficiency is Associated with Alterations in Bhattacharya S, Mereness J, Pryhuber G, Bara Response to H5N1 Influenza Vaccination. Asthma-like Phenotypes. A, Peterson D, Mariani T. Genome-wide Expression Profiling of Peripheral Blood from Misra R, Huyck H, Krenitsky D, Dunham A, Yee M, Gelein R, Mariani T, O’Reilly M. The Bronchopulmonary Dysplasia Subjects. Secor-Socha S, Quataert S, Wojciechowski W, Oxygen Environment at Birth is a Redox Yee M, Slaunwhite C, Lopez A, Lunger V, Regulator of Proper Alveolar Type II Cell. Bhattacharya S, Zhou Z, Yee M, Lopez AM, Solleti S, Bhattacharya S, Scheible K, Lunger VA, Buczynski B, Pryhuber G, Rangasamy T, O’Reilly M, Mariani T,

Abstracts Presented at Other National Research Meetings

Amin S, Karp J, Dorota K, Amin R, Wang H. simulation-based team training. International Biology Annual Meeting. Boston, MA. April, Developmental Defects of Enamel is Meeting on Simulation in Healthcare. Orlando, 2013. Influenced by Degree of Prematurity. 2014 FL. 2013. AADR/CADR Annual Meeting. Pasternack J, Dadiz R, McBeth R, Gerard J, Dardas M, Gill S, PhD2, Pryhuber G, Gill A, Scherzer D, Tiyyagura G, Zaveri P, Chang T, Bliek K, Wilmarth J, Brown K, Dadiz R, Lee Y-H, Guillet R. The Impact of Postnatal Auerbach M, Kessler D. Working Through Emrich P, Seaman M, Arnold C. Use of Antibiotics on Diversity of the Preterm Barriers to Simulation-based Just-in-Time Interdepartmental in situ Simulation as Part of Intestinal Microbiome. Presented at ESPR. Training and Competency Assessments for the PDSA Model for Patient Safety 2013. Infant Lumbar Punctures. Annual International Improvements. National Patient Safety Meeting on Simulation in Healthcare. San Foundation Annual Congress, Orlando. 2014. Emerich P, Brown K, Dadiz R. Assessment of Francisco. 2014. team ICARE values during simulated and Bhattacharya S, Zhou Z, Chu C., Yee M, actual deliveries. International Meeting on Ringo K, Norman R, Young JL. Extracellular Lopez A, Lunger V, Solleti S, Buczynski B, Simulation in Healthcare. Orlando, FL. 2013. matrix-associated protein CCN1 regulates Pryhuber G, Mariani T, O’Reilly M. Gordon shedding of endothelial ICAM-1. Research Conference on Lung Development, Finkelstin JN, Williams JP, Hernady E, Miller Experimental Biology Annual Meeting. Injury and Repair. New Hampshire. 2013. J, Stroyer B, Johnston CJ. ARG-1 is useful in Boston, MA. April, 2013. predicting the development of late pulmonary Buczynski BW, Yee M, Lawrence BP, fibrosis following irradiation. Radiation Scheible K. Accelerated maturation of O’Reilly MA. Neonatal hyperoxia promotes Research, New Orleans, LA. 2013. umbilical cord CD8+ T cells from infants born lung fibrosis independent of excessive prior to 35 weeks gestation. Cold Spring inflammation following influenza A virus Finkelstein JN, Williams JP, Reed C, Hernady Harbor 78th Symposium: Immunity and infection in mice. Society of Toxicologists. E, Miller J, Stroyer B, Johnston CJ. ARG-1 is Tolerance. May, 2013. 2013. useful in predicting the development of late pulmonary fibrosis following irradiation. Solleti S, Bhattacharya S. Rangel-Moreno J, Butler-O’Hara M, Reininger A, Dadiz R. Radiation Research, New Orleans, LA. Shimpi S. Bijli K, Lunger V, Lopez A, Randall Peripherally Inserted Central Cather Placement September, 2013. R, Rangasamy T. Rahman A, Mariani T. in the Neonate. Neonatal Advanced Practice SerpinE2 Deficiency is Associated with Nursing Forum. Washington, DC 2014. Leonard A, Rahman A, Fazal F. ER Thrombin-Mediated Activation of NF-?B and Chaperone BiP (GRP78) and Mitochondrial Alterations in Lung Lymphocyte Dadiz R, Schriefer J, Weinschreider J, Arnold chaperone Mortalin (mthsp75) Differentially Accumulation. Gordon Research Conference C, Pressman EK, Guillet R. Delivery room Regulate RelA/p65f Activation and on Lung Development, Injury and Repair. communication improves after implementing Endothelial Cell Inflammation. Experimental New Hampshire, 2013.

Golisano Children’s Hospital 2013 – 2014 Annual Report Recent Study Sections and Advisory Committee Memberships

Sanjiv B. Amin, MBBS, MD, MS • Board of Scientific Counselors, NIEHS, NIH • Reviewer for NIH Loan Repayment Proposals, 2010 – 2014 • Advisory Board Member, CounterACT Research Center of • CTSI/GCRC Advisory Committee member, University of Excellence, UMDNJ/Rutgers University Rochester

Ronnie Guillet, MD, PhD Patricia R. Chess, MD • Steering Committee member, Efficacy of Intravenous • Pediatric Planning Committee, American Thoracic Society; Levetiracetam in Neonatal Seizures, funded by FDA 2001 – present • DSMC member, Preterm Epo Neuroprotection (PENUT) • Research Grant Review Committee, American Lung Trial, funded by NINDS Association, 2001 – present • Chair, Research Grant Review Committee, American Lung Association, 2013, 2014 • American Lung Association Scientific Advisory Ruth A. Lawrence, MD Subcommittee: Awards and Grants Program, 2013, 2014 • Advisory Committee to promote breastfeeding among • Pediatric Research Society Grant reviewer 2009 – present clients at Early Head Start, Washington, DC; • URSMD, MD with Distinction in Research Committee 2011 • Pediatric Advisory Committee, FDA – present • Board of Directors, New York State Coalition for • UR, George Eastman Circle Advisory Committee 2011 – Breastfeeding present • Expert Panel, Baby Friendly National Program • Board of Directors, Mother’s Milk Cooperative • Breastfeeding Medicine, Editor in Chief • ABM Annual Summit, Chair, 2009-present Carl T. D’Angio, MD • Member, NIAID special Emphasis Panel, Medical Countermeasures for Biodefense and emerging Infectious Diseases, 2013 William Maniscalco, MD • Pediatric Academic Society Abstract Reviewer • Grant Reviewer, Strong Children’s Research Center, URMC

David A. Dean, PhD • Member, Nanotechnology Study Section, NIH, 2011-2015 • External advisory committee, program project grant on Thomas J. Mariani, PhD Pathophysiology of Alveolar Epithelial Lung Injury, • Permanent Member, NIH/Lung Injury Repair and Northwestern University, 2007-present Remodeling Scientific Review Group, 2012-2018 • Respiratory Tract Gene Therapy Committee member, American Society for Gene Therapy, 2010-2013 Robert H. Notter, MD, PhD • Solicited Ad Hoc Grant Reviewer for NIH and foundations

Michael A. O’Reilly, PhD Jacob N. Finkelstein, PhD • NIH, ad hoc reviewer, LIRR • Ad hoc Reviewer: National Cancer Institute (NCI), National Heart, Lung, and Blood Institute (NHLBI), National Institute Gloria S. Pryhuber, MD of Environmental Health Science (NIEHS) ONES Review • Grant Reviewer, NIH SEP/SRG KO1, KO8, R25, RO1 Panel, National Institute of and Immunologic Reviews (2 – 3 Study Sections per year) 2010 – present Diseases, James and Esther King Biomedical Research • URMC Committee Member: Clinical Research Review Program (State of Florida Biomedical Research Program), Process Improvement Team (CRRPIT) 2013 Tobacco Related Diseases Research Program (California) • Member F10A Study Section Timothy P. Stevens, MD, MPH • California National Primate Research Center, Davis CA • New York State Obstetric and Neonatal Collaborative • Advisory Committee, San Joaquin Valley Particulate Matter (NYSONQC) Steering Committee Member and Data Panel Research Center, Davis CA Chair 2008-present

Golisano Children’s Hospital 2013 – 2014 Annual Report Teaching Activities

Pediatric Residents

Faculty of the Division of Neonatology teach Pediatrics and Medicine-Pediatrics residents in the Neonatal Intensive Care Unit (NICU) at Golisano Children’s Hospital (GCH) and Pediatrics and Family Medicine residents in the Special Care Nursery (SCN) at Rochester General Hospital (RGH). The division faculty teach approximately 70-75 residents during rotations in the NICU and SCN. Clinical teaching activities include Attending Rounds (5 days/week), Work Rounds (7 days/week), Health Team Rounds (1 day/week), High-Risk Perinatology Rounds (1 day/week), NICU Pathology Rounds (1 day/4 weeks), and Ethics Rounds (1 day/4 weeks). Division faculty are active in teaching on the pediatric wards and in the Neonatal Resuscitation Program for residents in Pediatrics, Medicine-Pediatrics, , and Obstetrics and Gynecology.

Neonatal-Perinatal Medicine Fellowship Program

The three-year, ACGME-accredited, Neonatal-Perinatal Medicine Fellowship fully meets the requirements of the Neonatal-Perinatal Medicine board of the American Board of Pediatrics, and is certified for 8 fellows. The program provides intensive training in clinical care of high-risk newborns within a 13-county referral region in a 60-bed Level IV NICU (including ECMO, HFOV, and inhaled nitric oxide), a 12-bed Level II Special Care Nursery, and an 8- bed Level I nursery. Fellows acquire research experience, including study design, institutional review, study performance, analysis, presentation skills, and publication. They may choose a traditional basic science / clinical science / translational science path, or they may pursue alternate career development tracks while meeting the ABP requirements for scholarly activity. These options include degrees from the Department of Public Health Sciences, the Simon School of Business, and the Warner School of education, namely: Master of Public Health (MPH), Master of Science in Clinical Investigation (MS-CLI), Master of Science in Medical Management (MS-MM), or Master of Science in Health Professions Education (MS-HPE).

ACGME Fellows, Neonatal – Perinatal Medicine

Jayson Lingan, MD 1st year Medical School: University of the Philippines, Philippines : Geisinger Medical Center, Danville, PA Awards:

• T32 Recipient, Pediatric Research: Bench to Bedside to Curbside. 2014.

Research: Scholarship Oversight Committee: • The role of oxygen in closing the mitochondrial permeability transition pore and Mentor: George Porter, MD, PhD ultimately increasing mitochondrial function and cardiac myocyte differentiation Rita Dadiz, DO Michael O’Reilly, PhD Paul Brookes, PhD () Patricia Chess, MD (ad hoc) Presentations: • Lingan J. Management of patent ductus arteriosus among infants less than 28 weeks of gestation at birth. 2013 American Academy of Pediatrics National Conference and Exhibition. Orlando.

Golisano Children’s Hospital 2013 – 2014 Annual Report

Christina Sollinger, MD 1st year Medical School: St. George’s University, Grenada Residency: University of Medicine and of New Jersey, Newark, NJ Awards: • T32 Recipient, Pediatric Research: Bench to Bedside to Curbside. 2014. • William L. Bradford Fellowship. Strong Children’s Research Center, Department of Pediatrics, URMC. 2014. Research: Scholarship Oversight Committee: • Identify signaling pathways involved in cardiac/diaphragmatic development in Mentor: Kate Ackerman, MD CDH Rita Dadiz, DO Eric Small, PhD (Medicine, Aab CVRI) Faqian Li, BMed, PhD (Pathology) Patricia Chess, MD (ad hoc)

Sarah Volz, DO 1st year

Medical School: West Virginia School of Osteopathic Medicine, Lewisburg, WV Residency: Good Samaritan Hospital Medical Center, West Islip, NY

Research: Scholarship Oversight Committee: • To determine the efficacy of using a CMAC visualization tool to optimize Mentor: Judith Fonzi, PhD (Warner School) intubation skills in trainees Rita Dadiz, DO William Varade, MD Patricia Chess, MD (ad hoc)

Pratik Parikh, MBBS 2nd year Medical School: Pramukh Swami Medical College Residency: St. Peters University Hospital Awards: • T32 Recipient, Pediatric Research: Bench to Bedside to Curbside. 2012.

• Samuel W. Clausen Fellowship. Strong Children’s Research Center, Department of Pediatrics, URMC. 2014. • Travel Award, ESPR Annual Meeting, 2014. Research: Scholarship Oversight Committee: • Gene therapy of PDA in mouse model Mentor: David Dean, PhD Rita Dadiz, DO George Porter, MD, PhD Patricia Chess, MD (ad hoc) Presentations: • Parikh P, Alfieris G, Swartz M, Dean D. Gene expression of the ductus arterisus and aorta from neonates and infants. Eastern SPR, E-PAS2014:211, 3/22/14. Philadelphia PA, Platform presentation. • Parikh P, Alfieris G, Swartz M, Dean D. Gene expression of the ductus arterisus and aorta from neonates and infants. Pediatric Academic Societies Annual Meeting E-PAS20142730.4, Vancouver BC, May 4, 2014.

Srujana Rallabandi, MBBS 2nd year Medical School: Osmania Medical College Residency: East Carolina University Awards:

• Dean’s Fellow. School of Medicine and Dentistry, University of Rochester Medical Center. 2012. Research: Scholarship Oversight Committee: • Antioxidant effects of unconjugated bilirubin in neonates Mentor: Sanjiv Amin, MBBS Rita Dadiz, DO William Maniscalco, MD Mina Chung, MD () Patricia Chess, MD (ad hoc)

Golisano Children’s Hospital 2013 – 2014 Annual Report

Ahmad El Samra, MD 3rd year Medical School: American University of Beirut Residency: State University of New York Upstate Medical University Awards: • Travel Award, Academy of Pediatrics 82nd Perinatal and Developmental Medicine Symposium. June 2013. • William L. Bradford Fellowship. Strong Children’s Research Center, Department of Pediatrics, URMC. 2013. Grants: • El Samra A. 2012 Pediatric Research Award. The Effect of Prenatal Indomethacin on Kidney Function in Premature Infants. The Gerber Foundation. Fremont, MI. Principal Investigator. $20,000. Research: Scholarship Oversight Committee: • Urinary markers of renal dysfunction in the Neonate Mentor: Ronnie Guillet, MD, PhD Marc Lande, MD, MPH Rita Dadiz, DO Ayesa Mian, MD Patricia Chess, MD (ad hoc) Presentations: • El Samra A, Mian A, Lande M, Guillet R. The Effect of Prenatal Medications on Acute Kidney Injury in Premature Infants as Assessed by novel Urinary Biomarkers. American Academy of Pediatrics 82nd Perinatal and Developmental Medicine Symposium. Aspen, CO. June 2013. Poster presentation. • El Samra A, Main A, Lande M, Guillet G. Urinary biomarkers to evaluate for AKI in premature neonates exposed to prenatal indomethacin36th annual thruway conference, October 20, 2013. Buffalo NY. Oral presentation. • El Samra A, Main A, Lande M, Guillet G. Urinary biomarkers to evaluate for AKI in premature neonates exposed to prenatal indomethacin. Eastern SPR annual meeting, E-PAS2014:381, March 23, 2014. Philadelphia PA. Poster presentation. • El Samra A, Main A, Lande M, Guillet G. Urinary biomarkers to evaluate for AKI in premature neonates exposed to prenatal indomethacin. Pediatric Academic Societies annual meeting, E-PAS20142908.88 May 4, 2014. Vancouver BC. Poster presentation.

Kunal Gupta, MBBS 3rd year Medical School: Government Medical College and Hospital Residency: Post Graduate Institute of and Research (India) Awards: • T32 Recipient, Pediatric Research: Bench to Bedside to Curbside. 2012. • Samuel W. Clausen Fellowship. Strong Children’s Research Center, Department of Pediatrics, University of Rochester Medical Center. 2012. Research: Scholarship Oversight Committee: • Effect of lipid intake on direct hyperbilirubinemia in late preterm and term Mentor: Sanjiv Amin, MBBS infants with gastrointestinal surgical problems Marilyn Brown, MD Rita Dadiz, DO Patricia Chess, MD (ad hoc) Presentations: • Gupta K, Amin S. Intravenous Glucose and Lipid Intake Are Determinants of Parenteral Nutrition Associated Cholestasis in Premature Infants. Pediatric Academic Societies Annual Meeting. Washington, DC. 5/4 – 5/7/2013. Poster presentation. • Gupta K, Bean S, Wang H, Chess P, Amin S. Effect of Lipid Intake on Direct Hyperbilirubinemia in Late Preterm and Term Infants with Gastrointestinal Surgical Problems. 35th American Academy of Pediatrics Perinatal Research Mid-Atlantic Conference. Hershey, PA. September 2012. Oral presentation. Publications: • Gupta K, Amin S. Management of , Peri-FACTS, September 1, 2012, Case #1008, 2012 • Gupta K, Amin S. Physiology of Neonatal Jaundice, Peri-FACTS, September 1, 2012, Case #1006, 2012 • Gupta K, Amin S, Wang H. Parenteral nutrition associated cholestasis in premature infants: role of macronutrients. Journal of Parenteral and Enteral Nutrition. Submitted 06/2014.

Golisano Children’s Hospital 2013 – 2014 Annual Report

Syed Shah, MBBS 3rd year Medical School: Punjab Medical College Residency: Bronx-Lebanon Hospital Center Awards: • Travel Award, ESPR Annual Meeting, 2014.

• Travel Award, PAS Annual Meeting, 2014. Research: Scholarship Oversight Committee: • Correlation of cord blood regulatory T cell number and function with premature Mentor: Gloria Pryhuber, MD birth and severity of lung disease prior to discharge Deborah Fowell, PhD, Microbiology and Immunology Rita Dadiz, DO Patricia Chess, MD (ad hoc) Presentations: • Shah SK, Misra R, Huyck H, Wang H, Stevens T, Pryhuber G. Chorioanmionitis increases core blood CD4+T lymphocyte IL-6 and TNF-a response which predict development of BPD in preterm babies. 36th Annual Thruway Conference, October 10, 2013, Buffalo NY – oral. • Shah SK, Misra R, Fowell D, Wang H, Scheible K, Stevens TP, Misra S, Huyck H, Mariani T, Pryhuber G. Chorolamnionitis increase preterm cord blood CD+ T lymphocyte IL-6 and TNF-a response which predicts the development of bronchopulmonary dysplasia (BPD). Pediatric Academic Societies annual meeting, E-PAS2014:3185.3. Vancouver BC, May 4, 2014. Platform presentation. • Shah SK, Misra R, Fowell D, Wang H, Scheible K, Stevens TP, Misra S, Huyck H, Mariani T, Pryhuber G. Chorolamnionitis increase preterm cord blood CD+ T lymphocyte IL-6 and TNF-a response which predicts the development of bronchopulmonary dysplasia (BPD). ESPR annual meeting, E-PAS2014:3185.3. Philadelphia PA, March 21, 2014. Poster presentation Publications: • Shah S, Misra R, Fowell D, Wang H, Scheible K, Katzman P, Misra S, Huyck H, Mariani T, Pryhuber G. Preterm cord blood CD4+ T cell exhibit increased IL-6 production in chorioamnionitis and decreased T regulatory cells in bronchopulmonary dysplasia. Human Immunology Journal –manuscript submitted 6-2014

Basic Science Teaching in Graduate / Undergraduate Courses

In addition to clinical teaching activities, several faculty in the Division of Neonatology have secondary appointments in other departments of the University of Rochester, and participate in teaching in graduate and undergraduate courses. These faculty members include Drs. Chess, Dean, Finkelstein, Mariani, O’Reilly, Pryhuber, and Rahman, who lecture in and/or direct graduate or undergraduate courses in the Toxicology Program and in the Departments of Environmental Medicine, Biomedical Engineering, and Chemical Engineering. Division faculty also in lecture to medical students during courses in the Double Helix Curriculum.

Teaching Honors and Awards

• Rita Dadiz, DO: Third place for research abstract, International Meeting on Simulation in Healthcare, 2014 • William Maniscalco, MD: Junior Faculty Mentoring Award 2014

Major Educational Presentations and Programs

Sanjiv B. Amin, MBBS, MD, MS • Invited Speaker, AAP and NYEHDI Collaboration to Create • Invited Speaker, Bottle or Breast: Weight Gain, National a Physician Residency Training Curriculum, EHDI Neonatal Conference Meeting (NEOCON), India, 2012 Conference, Glendale, AZ, 2013 • Invited Speaker, Auditory Evaluation of Neonates, Neonatal • Bilirubin Metabolism, Neonatal Medical Knowledge Fellow Medical Knowledge Seminar Series, New York State Early Seminar Series, 2013 Hearing Detection and Intervention Conference, Syracuse, • Bilirubin Pathophysiology, Neonatal Medical Knowledge NY, 2012 Fellow Seminar Series, 2013 • Invited Speaker, Bilirubin Induced Neurotoxicity, Grand • Neonatal Clinical Research Seminar, Bilirubin-induced Rounds, Michigan State University, East Lansing, MI 2012 auditory toxicity in late preterm and term infants – INDO- • Informal Communication Assessment: Critical Information US study. 2014 that can help in late-identified hearing loss, 2012

Golisano Children’s Hospital 2013 – 2014 Annual Report Melissa Carman, MD • Session Moderator, Neonatal Patient-Oriented Research • The Use of Pulse Oximetry in Newborn Screen for Platform, Pediatric Academic Society Meeting, Washington, Congenital Heart Disease, Pediatric Grand Rounds, DC, 2013 Rochester General Hospital, October 2012 • Next Accreditation System, Don’t Panic, Don’t • Apnea and Control of Breathing, NMKB, URMC, Procrastinate. PAS, 2014 November 2012 • Screening for Congenital Heart Disease, Perinatal Outreach Visit, FF Thompson Hospital, Canandaigua, NY, September, Rita Dadiz, DO 2012; Geneva General Hospital, Geneva, NY, November, • Obstetric and neonatal multidisciplinary simulation-based 2012 team training, URMC, 2007-present • Neonatal Abstinence Syndrome, Perinatal Outreach Visit, • Delivery room and newborn nursery simulations, URMC, Arnot Ogden Hospital, Corning, NY, February 2013 2008 – present • Neonatal Hypoglycemia, Perinatal Outreach Visit, Corning • In-situ NICU mock codes, URMC, 2009 – present Hospital, Corning, NY, February 2013 • Training for the unexpected, Simulation course facilitator, • Multiple NRP courses at both RGH and URMC, teaching Center for Obstetrics and Gynecology Simulation, URMC, both community and resident physician providers, 2012- 2009 – present 2013 • Neonatal Simulations, Pediatric Residents’ Skills Block, URMC, 2012 • Disclosing Adverse Events, Medical Student Workshop, URSMD, 2012 Patricia R. Chess, MD • Simulation-Based Learning: Assessing Your Learners and • Genes to Generations 3rd year medical student course, Program, Dean’s Teaching Fellowship Program, URSMD, Pulmonary Development lecturer, URSMD, 2001-present 2012 • Nursing Care of the High Risk Neonate (course 436) • Debriefing: Learner-Centered Feedback for Education, URSON, Follow-up of the NICU graduate, ROP, 2007- Patient Care and Quality Improvement; Workshop, Pediatric present Academic Societies, 2012, 2013 • Academic Core Curriculum, Ten Minute Talks, URMC, • Shoulder dystocia: a safer way forward (webinar). NYS Pediatrics, 2006 – present Perinatal Quality Collaborative of the New York State • Feedback and Evaluation, Pediatric and Medicine-Pediatrics Department of Health and New York State Partnership for Residency Competency Core, URMC, 2007 – present Patients of the US Department of Health and Human • Use of iNO/ECMO/High frequency NMKB, URMC, 2007 – Services, 2013 present • Coming to the table: debriefing for patient safety (webinar). • Effect of Timing of Elective Deliveries and admission to the NYS Perinatal Quality Collaborative of the New York State Neonatal Intensive Care Unit, Cayuga Medical Center, Department of Health and New York State Partnership for Ithaca NY, 2011 Patients of the US Department of Health and Human • CDH: New approaches to an old problem, URMC, Services, 2013 December 2012 • Improving interdisciplinary communication for patient • Cyanotic Congenital Heart Disease, Unity Grand Rounds, safety. Advances in Clinical Perinatal Medicine, Regional Unity Hospital, Rochester, NY, February 2012 Perinatal Symposium, Syracuse. 2013 • Symposium Director, High Frequency Oscillatory • Methemoglobin and the response to iNO in PPHN. Thruway Ventilation Symposium: Hands on Interactive Workshop, Conference, Buffalo. 2013 Northeastern US Regional Symposium for Neonatologists, • Simulation-based training to improve obstetric and pediatric Respiratory Therapists, and Neonatal Nurse Practitioners, communication. Research Update, University of Miami URMC, May 2012 Miller School of Medicine. Miami. 2013 • Moderator, American Academy of Pediatrics Perinatal • Team building through the use of effective communication Section Mid-Atlantic Conference, Hershey, PA 2012 skills. Jointly sponsored by the Public Service Center, Office • Congenital Diaphragmatic Hernia: New approaches to an old of Undergraduate Biology, and Biology Service Leaders. problem, Harriet Davis Teaching Day, URMC, 2012 Cornell University. 2014 • Parenteral Nutrition-Induced Cholestatic Jaundice: The role • Patient counseling and education. NY State Perinatal Quality of restricted IV Lipid and Omega-3 Fat Emulsion: 6 years’ Collaborative of the NY State Department of Health, experience at UR, NYS Thruway Conference, URMC, 2012 Obstetric Prenatal Education Project. 2014 • Persistent Pulmonary Hypertension and ECMO: The Times • Giving Bad News. Fellowship workshop. 2014 They Are a Changin’, Contemporary Management of Neonatal Pulmonary Disorders, Tempe, AZ, 2012 • Congenital Diaphragmatic Hernia: New approaches to an old problem: Contemporary Management of Neonatal Pulmonary Disorders, Tempe, AZ, 2012

Golisano Children’s Hospital 2013 – 2014 Annual Report Carl T. D’Angio, MD • Visiting Professor, University of Mississippi, January 2013 • Ethics and Professional Integrity in Research (IND o Neonatal Seizures. University of Mississippi, 501/506), Course Director, URMC, 2013 – present Neonatology Research Conference • NICHD Neonatal Research Network Update: Past o Perinatal Regionalization. University of Experience, Future Opportunities. NYS Thruway Mississippi, Perinatal Collaborative Care Conference, URMC, 2012 Conference • Genes to Generations (medical student course), Pulmonary o Breaking Bad News. University of Mississippi, Biology Lecturer, URMC Research Ethics/Integrity (IND Pediatric Grand Rounds 503), Session Facilitator, URMC, 2005 – 2012 • TOX 560: Therapeutic Hypothermia, 2012 • Statistics Lecturer, Academic Core Curriculum, URMC, • The Transition from Fetus to Newborn; McGill University Pediatrics, 2005 – present 2012, Cornell University, Ithaca, NY, 2012 • Process of Discovery, 4th year medical student small group facilitator, URMC, 2012, 2013 • Therapeutic Hypothermia, Arnot Ogden Medical Center David A. Dean, PhD teleconference, 2013 • Development of cell-specific targeting approaches for • Junior Faculty Academic Curriculum Director, 2012 – pulmonary gene therapy, Mary Babb Randolph Cancer present Center, West Virginia University, Morgantown, WV, 2012 • Intracellular trafficking of DNA and its impact on gene therapy, Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, 2012 Carl Johnston, PhD • Cell-specific targeting strategies for electroporation- • Pulmonary Inflammation. TOX521 Core Course, one 1 mediated gene delivery in the lung, International Scientific lecture, URMC, 2010 – present Workshop and Postgraduate Course on Electroporation Based Technologies and Treatments, Ljublijana, Slovenia, 2012 • Electroporation-mediated gene delivery to cells and animals, Nirupama Laroia, MD Gene Transfer Technologies into Multicellular Eukaryotes, • Normal and Sick Newborn, Physician Assistant Program, Vienna, Austria, 2013 Rochester Institute of Technology, 2000 – Present • Molecular considerations for effective gene therapy, Student • Neonatal , Neonatal Nurse Practitioner Course, Research Day Keynote Speaker, SUNY Upstate Medical URSON, 1999 – Present University, Syracuse, NY, 2013

Ruth A. Lawrence, MD Fabeha Fazal, PhD • Annual National Broadcast for World Breastfeeding Week, • Actin in Action: Role in Lung Vascular Inflammation, Lung School of Public Health, SUNY Albany, August 2008 -2012 Biology Research Seminar Series, Lung Biology and • Session Moderator, International Society Research in Disease Program, Department of Pediatrics, URMC, 2012 Human Milk and Lactation, Trieste, Italy, September 2012 • Academy of Breastfeeding Medicine Founders Meeting, September 2012 • Kellogg Foundation Board Conference, September 2012 Jacob N. Finkelstein, PhD • STEPPS Program for Malpractice Relief, URMC. November • Co-director, Advanced Pulmonary Toxicology (TOX 564) 2012 • Lecture, core Toxicology (TOX) 521) • The Revolution in Infant Feeding, 3rd Wednesday CME • Participant, PATH 509: Environmental Pathology Speaker, URMC, November, 2012 • Epidemiology of Breastfeeding, URMC, February 2013 • The Revolution in Infant Feeding, Grand Rounds, URMC, January 2013 Ronnie Guillet, MD, PhD • Women’s Health / Breastfeeding, 2nd Year Medical Students, • PROPHENO - Update, NESTT Meeting, San Francisco, CA, URMC, February 2013 2010, 2011, 2012 • Newest Information on Breastfeeding, Brooklyn Hospital, • Neonatal Seizures: What’s Old, What’s New, 15th Annual A 2013 Day with the Newborn, St. Christopher’s Hospital for • Breastfeeding Today, Cobb & Douglas Public Health, Children, Philadelphia, PA, 2012 Atlanta, GA, May 2013 • From Fetus to Newborn, McGill University Teleconference, 2012

Golisano Children’s Hospital 2013 – 2014 Annual Report William M. Maniscalco, MD • Invited Speaker, Assessing Growth in Preterm Infants: Does • Care of newborns <28 weeks, USAID Conference, Moscow, Quality Matter? Annual Townsend Teaching Day, Rochester Russia, May 2012 General Hospital, 2014 • Invited Speaker, Neonatal Emergencies, STEP EMS Conference, Rochester, 2014

Thomas J. Mariani, PhD • Genomics of Lung Development and Disease, Invited Speaker, Pediatric Academic Societies Annual Meeting, Michael A. O’Reilly, PhD Washington, DC, 2013 • TOX 594: Gene Environment Interactions Course Co- • Genomics of Bronchopulmonary Dysplasia, Invited Speaker, Director; oversaw 13 lectures ATS International Conference, Philadelphia, PA, 2013 • TOX 521: Toxicology Core Course; Course Co-Director; • Genomics of Lung Development and Bronchopulmonary gave 4 lectures Dysplasia, Invited Speaker, Brasel Basic Science Lecture, • PM425: Health promotion and preventive medicine, 1 Harbor-UCLA Medical Center, 2013 lecture • Integrating Systems-Level Analysis to Define the Molecular Bases of Respiratory Disease, Invited Speaker, Computational Biology Seminar, IBM Research, Yorktown Heights, NY, 2013 Dale L. Phelps, MD • Bioinformatics Workshop, Co-Director and Lecturer, • Retinopathy of Prematurity: Mechanism and Prevention; Queen’s University, Kingston, ON, 2012 Web Symposium Sponsored by the American Association of • Life and Breath, Mini-Medical School, URMC, Department Pediatric Ophthalmology and Strabismus, December 2012 of Pediatrics, 2012 • Retinopathy of Prematurity, an Update; NEO the Conference • Neonatal Origins of Adult Pulmonary Disease, Session for Neonatology; Sponsored by NEDNAX Center for Chair, American Thoracic Society International Conference, Research, Education and Quality; Orlando, FL, February 2012 2013 • Lung Development: Bench to Bedside, Session Chair, American Thoracic Society International Conference, 2012

• Genomics of BPD, Invited Speaker, Department of Pediatrics, University of Alabama, Birmingham, AL, 2012 Gloria S. Pryhuber, MD • Mechanistic insights into a COPD candidate gene, Invited • Poster Design: Presenting Your Hard Work, Academic Core Speaker, Department of Medicine, University of Alabama, Curriculum, URMC, 2011, 2013 Birmingham, AL, 2012 • TOX 522 Organ Systems Toxicology: Section on Pulmonary • Invited Speaker, Genetic Contributions to Lung Disease in Toxicology, 2001-present Preterm Infants, PAS, 2014 • Each year, teach 2-3 of the following: Lung Anatomy, • Session Chair, The Systems Biology of Complex and Rare Physiology and Experimental Models; Acute Lung Pediatric Diseases, PAS, 2014 Inflammation, Epithelial Injury and Repair; Cellular • Invited Speaker and Conference Vice-Chair, Gordon Mechanisms of Lung Injury and Repair Research Conference on Lung Development, Injury and • The Prematurity and Respiratory Outcomes Program Repair, 2013 (PROP): Objectives, Design, and Implementation; Pediatric Grand Rounds; URMC; 2012

Jeffrey Meyers, MD • Ca/Phos/Mg Metabolism, Fellow Neonatal Medical Arshad Rahman, MD Knowledge Base Curriculum, URMC, 2012 • Session Chair, Novel Airway Epithelial Barrier and Immune • Host Defense of the Neonate, Fellow Neonatal Medical Responses, American Thoracic Society International Knowledge Base Curriculum, URMC, 2012 Conference, San Francisco, CA, 2012 • Pulmonary Development, URSMD, 2012 • Blocking NF-κB: An Inflammatory Issue; Cell Biology, • Fellow’s Neonatal Medical Knowledge Base Curriculum, Neurobiology and Anatomy Graduate Program; Loyola University of Rochester: Calcium, Phosphorus & University Medical Center; Maywood, IL, 2012 Magnesium Metabolism, 2012, 2013; Host Defense of the • A Novel Role of Autophagy in Endothelial Cell Neonate, 2012 Inflammation and Lung Vascular Injury, American Thoracic • Genes to Generations 3rd year medical student course, Society International Conference, Philadelphia, PA, 2013 Pulmonary Development, University of Rochester, 2012, • Mechanisms of Endothelial Cell Inflammation and Acute 2014 Lung Injury. Research In Progress Seminar, Department of Microbiology and Immunology, URMC. 2014

Golisano Children’s Hospital 2013 – 2014 Annual Report • Mechanisms of Endothelial Cell Inflammation and Acute • Data Update: Relationships between Survey Results and Lung Injury, Department of Biochemistry, Aligarh Muslim SPDS-NICU Module Data, NYS Perinatal Quality University, India. 2014 Collaborative, Albany, NY. October 2012 • Screening for Congenital Heart Disease in Asymptomatic nd Patients: Building a Program, 32 Annual Townsend Teaching Day, Rochester General Hospital, May 2012 Kristin Scheible, MD • Invited Speaker, Impediments and Solutions to Feeding Our • Neonatal Immunity, Fellow Neonatal Medical Knowledge Tiny Babies, Garden City, NY, March 2013 Base Curriculum, URMC, 2012 • PDA Ligation and Pain Control in the NICU, New Fellows Orientation, Neonatology, URMC, 2012 • Time Management in Neonatology Fellowship, New Robert J. Swantz, MD Fellows Orientation, Neonatology, URMC, 2012 • Perinatal Outreach Program, yearly CME talks, Newark- Wayne Community Hospital, Newark, NY, 1995 – present • Perinatal Outreach Program, yearly CME talks, Nicholas Noyes Memorial Hospital, Dansville, NY, 2000 – present Laurie Steiner, MD • Course Director PED300: Pediatric Clerkship, URSMD, • Unbiased Identification of Functional Barrier Insulators in 2000 – present Primary Human Erythroid Cells, Experimental Hematology • Course Director PEDEXT: Pediatric Sub-Internship, Seminar, URMC, February 2012 URSMD, 2000 – Present • Unbiased Identification of Functional Barrier Insulators in • Genes to Generations, lecturer on costs, Primary Human Erythroid Cells, Neonatology Research URSMD, 2000 - Present Seminar, URMC, October 2012 • Effective Teaching in the Clinical Setting, lecturer in • CTCF, Cohesin, and Erythoid Development; Forbes Visiting Academic Core Curriculum, URMC, 2005 - present Scholar Program, URMC, 2012 • Effective Teaching, workshop for second year pediatric residents, URSMD, 1996 – Present

Timothy P. Stevens, MD, MPH • NICU / Neonatology Web Site: a resource for resident, Jennifer L. Young, PhD fellow and nursing education and clinical care. It includes a • Matricellular Protein CCN1 Regulates Shedding of medication reference, resident guide to common neonatal Endothelial 1CAM1 Toxicology Research Day, URMC, care and on-call issues, as well as PowerPoint presentations 2012 on common neonatal diseases and links to extramural pediatric and neonatal resources, 2004 – present • Perinatal Outreach Program, semiannual CME talks, FF Thompson Hospital, Canandaigua, NY, 2001 - present

Service Activities

Sanjiv B. Amin, MBBS, MD, MS • Prenatal Diagnosis Committee • New York Chapter Champion, Newborn Hearing Committee, American Academy of Pediatrics • GCRC (CTSI) Advisory Committee Member, URMC • New Born Hearing Committee, URMC Patricia R. Chess, MD • ROP Oversight Committee Member, URMC • Fellowship Director, Neonatal-Perinatal Medicine • Manuscript reviewer: 8 to 10 manuscripts for peer-review Fellowship Program journals per year • Chair of Pediatrics, Unity Hospital • Pediatric Academic Society • Medical Director, ECMO Program, ELSO Registry • APS/SPR Student Research Program Steering Committee Melissa Carmen, MD • Graduate Medical Education Committee Member • Regional Perinatal Outreach Coordinator • UR Advisory Parent Council • Finger Lakes Regional Perinatal Resuscitation Program, • UR George Eastman Circle Advisory Council URMC • Assistant Coordinator, Neonatal Resuscitation Program, URMC

Golisano Children’s Hospital 2013 – 2014 Annual Report Rita Dadiz, DO • CTIS Education Directorate, URMC • Medical Advisor, Neonatal Resuscitation Program, URMC • URMC Mentorship Award Committee • Program Faculty, International Meeting on Simulation in • Medical Executive Committee, Highland Hospital Healthcare • Perinatal Morbidity and Mortality Committee, Co-Chair, • Program Faculty, International Meeting on Simulation in Highland Hospital Healthcare • Perinatal Practice Committee, Highland Hospital • Member, Clinical Education Advisory Board, Center for Experiential Learning, URMC

Nirupama Laroia, MD • Committee Chair, India Giani Fund, under the International Carl T. D’Angio, MD Health Section of the AAP • Associate Chair, Board 5, Research Subjects Review Board, University of Rochester • Faculty Senate Research Policy Committee, UR • Society for Pediatric Research Fellows Research Awards Ruth A. Lawrence, MD Selection Committee, Clinical Team • Consultant, Food and Drug Administration Advisory Council

David A. Dean, PhD • Abstract Reviewer, American Society for Gene Therapy William M. Maniscalco, MD • Editorial Board Member, American Journal of Physiology: • Member, Pediatric Tenure and Promotion Committee, Lung Cellular and Molecular Physiology URMC • Editorial Board Member, Experimental Biology and Medicine

Thomas J. Mariani, PhD • Member, ATS RCMB Nominating Committee Fabeha Fazal, PhD • Director and Lecturer, Transcriptomics and Integrative • Reviewer, American Journal of Physiology: Heart and Genomics Interest Group, University of Rochester Circulatory Physiology • Editorial Board, American Journal of Respiratory Cell and • Reviewer, Journal of Biochemistry and Molecular Biology Molecular Biology • Reviewer, Frontiers in Bioscience: Molecular Biology in • Editorial Board, American Journal of Physiology, Lung Cell Clinical Practice and Molecular Biology • Reviewer, Chemical Research in Toxicology • Member, Faculty Search Committees; Microbiology and • Reviewer, Respiratory Research Immunology, Biostatistics and Computational Biology; • Reviewer, International Journal of Biochemistry and Cell URMC Biology • Member, ATS RCMB Executive Committee • Reviewer, Experimental Cell Research

Jeffrey Meyers, MD Jacob N. Finkelstein, PhD • OB service Team, Committee Member, URMC • Director of Faculty Development Program, Environmental • Perinatal Mortality Conference, Committee Member, URMC Health Sciences Center, URMC • Policy Committee, Environmental Health Sciences Center,

URMC • Steering Committee, Pulmonary Training Grant Michael A. O’Reilly, PhD • Steering Committee, Toxicology Training Program • Editorial Board, American Journal of Physiology: Lung Cellular and Molecular Physiology • Editorial Board, American Journal of Respiratory Cell and Molecular Biology Ronnie Guillet, MD, PhD • Board of Directors, March of Dimes Finger Lakes Chapter • Perinatal Morbidity and Mortality Committee, URMC • Prenatal Diagnosis Committee, URMC

Golisano Children’s Hospital 2013 – 2014 Annual Report Dale L. Phelps, MD • Editorial Board, Science of Advanced Materials • Member, AAO/AAP/AAPOS Joint Statement on ROP • Editorial Board, World Journal of Biological Chemistry Screening • Editorial Board, BioMed Research International • Member, DSMB for the Division of Lung Diseases Clinically Oriented Research. NIH-NHLBI • Member, DSMC for the Maternal Fetal Medicine Network. NIH-NICHD Timothy P. Stevens, MD, MPH • Member, DSMC for the NIH-NEI PEDIG Group Trials: • New York State Regional Perinatal Center Steering Multiple Trials Group in Pediatric Ophthalmology Committee • Medical Director, NICU, URMC

Gloria S. Pryhuber, MD • Director, Histology Services, Departments of Pediatrics and Robert J. Swantz, MD Environmental Services, URMC • Pediatric Education Committee, URMC • Abstract Referee, Pediatric Academic Society Annual • Medical Student Promotion and Review Board, URSMD Meeting • Session Moderator, Pediatric Academic Society Annual Meeting • Session Moderator, Eastern Society for Pediatric Research Annual Meeting Jennifer L. Young, PhD • Abstract/Poster Reviewer, Toxicology Research Day, URMC

Arshad Rahman, PhD • Editorial Board, American Journal of Physiology: Lung Cellular and Molecular Physiology

Three-Year Bibliography

2011 Jan. 39(1):55-8. Dadiz R, Guillet R. Interdisciplinary education: Improving communication and Albert S, Kubiak B, Vieau C, Roy SK, Bhattacharya S, Tyagi S, Srisuma S, DeMeo teamwork amongst pediatric and obstetric CiRocco J, Gatto L, Young J, et al. DL, Shapiro SD, Bueno R, Silverman EK, providers. NeoReviews 2011; 12(2):e63-8. Comparison of Open Lung Modes with Low Reilly JJ, Mariani TJ. Peripheral blood gene Tidal Volumes in a Porcine Lung Injury expression profiles in COPD subjects. J Clin Dadiz R. Update on Newborn Resuscitation, Model. J Surg Res. Mar: 166(1):59-20. 2011. Bioinformatics 2011; 1(12). Case #980. The University of Rochester Medical Center, Peri-FACTS Education Amin SB, Myers G, Wang H. Association Brehm, JM, Hagiwara K, Tesfaigzi Y, Bruse Program, University of Rochester Medical between neonatal iron overload and early S, Mariani T, Bhattacharya S, Boutaoui N, Center, Rochester, NY, 2012. human brain development in premature Ziniti J, Soto- Quiros M, Avila L, Cho M, infants. J Autism Dev Disorder. 2011, Nov, Himes B, Litonjua A, Jacobson F, Bakke P, Dean DA, Barravecchia, B, Danziger, Lin, X. 1455-63. Gulsvik A, Anderson W, Lomas D, Forno E, Use of Electroporation for efficacious gene Datta S, Silverman E, Celedon J. Identification delivery to the lungs. ECS Trans 35:167-177. Amin SB, Smith T, Wang H. Is Neonatal of FGF7 as a novel susceptibility locus for 2011. Jaundice associated with Autism Spectrum chronic obstructive pulmonary disease. Disorders: a systematic review. J Autism Dev Thorax. 2011 Dec; 66(12): 1085-90. Gewandter JS, Bambara RA, O’Reilly MA. Disorder. 2011, 41(11):1455-63. The RNA surveillance protein SMG1 activates D’Angio CT, Heyne RJ, Duara S, Holmes LC, p53 in response to DNA double strand breaks Amin SB, Lamola AA. Newborn jaundice O’Shea TM, Wang H, Wang D, Sanchez PJ, but not exogenously oxidized mRNA. Cell technologies, unbound bilirubin and bilirubin Welliver RC, Ryan RM, Schnabel KC, Hall Cycle. 10: 2561 - 2567. 2011. binding capacity in neonates. J Autism Dev CB; for the Premature Infant Vaccine Disorder. 2011. 41(11):1455-63. Collaborative. Immunogenicity of trivalent Guillet R, Dees R. Will my baby walk?: The influenza vaccine in extremely-low-birth- predictive value of cranial imaging. Amin SB, Miravalle N. Effect of ibuprofen on weight, premature versus term infants. Pediatr Neurology, 2011 Jun14; 76(24):2048-9. bilirubin-albumin binding affinity in premature Infect Dis J 2011; 30(7):570-574. PMID: 21670432 infants. Journal of Perinatal Medicine. 2011

Golisano Children’s Hospital 2013 – 2014 Annual Report Guillet R, Kwon JM, Chen S, McDermott M. pulmonary disease. J Immunology 2011; Lawrence RA. Global science and global Urine phenobarbital drug screening: Potential 187:987-998. PMCID: 3131437 profits. Breastfeeding Medicine. 2011. use for compliance assessment in neonates. J 6(4)161-2. Child Neurology. 2012 Feb; 27(2):200-3. Epub Jaacks LM, Young MF, Essley BV, McNanley 2011 Sep 29. TJ, Cooper EM, Pressman EK, McIOntyre Lawrence RM, Lawrence RA. Breastfeeding: AW, Orlando MS, Abkowitz JL, Guillet R, more than just good nutrition. American Han X, Gelein R, Corson N, Finkelstein JN, O’Brien KO. Placental Expression of the Academy of Pediatrics. 2011; 32(7)267-280. et al. Validation of an LDH assay for assessing Heme Transporter, Feline Leukemia Virus nanoparticle toxicity. Toxicology. 2011; Subgroup C Receptor, Is Related to Maternal Li X, Wang S, Li Y, Deng C, Steiner LA, 287(1-3):99-104. Iron Status in Pregnant Adolescents. J Nut, Xiao H, Wu C, Bungert J, Gallagher PG, 2011 Jul;141(7): 1267-72. Felsenfeld G, Qiu Y, Huang S. Chromatin Hersh CP, Silverman EK, Gascon J, boundaries require functional collaboration Bhattacharya S, Klanderman BJ, Litonjua Johnston CJ, Manning C, Hernady E, Reed C, between the hSET1 and NURF complexes. AA, Lefebvre V, Sparrow D, Reilly JJ, Thurston SW, Finkelstein JN, Williams JP. Blood. 2011 Aug 4; 118(5):1386-94. Epub Anderson WH, Lomas DA, Mariani TJ. Effect of total body irradiation on late lung 2011 Jun 8. SOX5 is a Candidae Gene for COPD effects: Hidden dangers. Int J Radiat Boil 2011 susceptibility and is Necessary for Lung Aug; 82(8):902-13. Lin X, Dean DA. Gene Therapy for Development. Am. J of Resp and Critical Care ALI/ARDS. Critical Care . 2011 Jul; Me. 2011; Epub 2011 Feb 17. Johnston CJ, Manning C, Hernady E, 27(3):705-18. Finkelstein JN, et al. “Effect of total body Himes BE, Klanderman B, Ziniti J, Senter- irradiation on late lung effects: hidden McGrath-Morrow S, Lauer T, Collaco M, Yee Sylvia J, Soto-Quiros ME, Avila L, Celedon dangers.” International Journal of Radiation M, O’Reilly MA, Mitzner W, Neptune E, JC, Lang C, Mariani TJ, Lasky-Su J, Hersh Biology. 2011. 87(8): 902-13. Wise R, Biswall S. Neonatal hyperoxia CP Raby BA, Silverman EK, Weiss ST, contributes additively to cigarette smoke- Demeo DL. Association of SERPINE2 with Kwon JM, Guillet R, Shankaran S, Laptook induced COPD changes in adult mice. Am J Asthma. Chest, 2011; Epub 2011 Mar 24. AR, McDonald SA, Ehrenkranz RA, Tyson Respir Cell Mol Biol. 2011; 45: 610-616. JE, O’Shea TM, Goldberg RN, Donovan EF, Hintz SR; Stevenson DK, Yao Q; Wong RJ, Fanaroff AA, Poole WK, Higgins RD, Walsh Melen E, Kho AT, Sharma S, Gaedigk R, Das A; Van Meurs KP, Morris GH, Tyson JE, MC, for the Eunice Kennedy Shriver NICHD Leeder JS, Mariani TJ, Carvey VJ, Weiss ST, Oh W, Poole WK, Phelps DL, McDavid GE, Neonatal Research Network. Clinical Seizures Tantisira KG. Expression analysis of asthma Grisby C. Higgins RD, Eunice Kennedy in Neonatal Hypoxic-Ischemic candidate genes during human and murine Shriver National institute of Child Health and Encephalopathy Have No Independent Impact lung development. Resp Res. 2011; 12(1):86. Human Development Neonatal Research On Neurodevelopmental Outcome: Secondary Network. Is phototherapy exposure associated Analyses of Data from the NRN Hypothermia Papuga MO, Kwon E, You Z, Rubery PT, with better or worse outcomes in 501- to 1000- Trial, J Child Neurology 2011 Mar; 26 Dougherty PE, Pryhuber G, Beck CA, Hilton g-birth-weight infants? Acta Paediatr. 2011; (3):322-8. MJ, Awad HA, Schwarz EM. TNF is required 100(7):960-5. for the induction but not the maintenance of Lawrence RA. Global Science and Global compression-induced BME signals in murine Holten-Andersen N, Henderson JM, Walther Profits. Breastfeeding Medicine. 2011; 6(4):1. tail vertebrae: limitations of anti-TNF therapy FJ, Waring AJ, Ruchala P, Notter RH, Lee for degenerative disc disease. J Ortho Res. KY. KL4 peptide induces reversible collapse Lawrence RA. A Tribute in Memoriam. 2011; 29(9):1367-1374. structures on different length scales in Breastfeeding Medicine. 2011 July; 6(4):3. monolayers containing anionic POPG. Pilon AM, Ajay SS, Kumar SA, Steiner LA, Biophys J, 2011 Dec 21;101(12):2957-65. doi: Lawrence RA. Factors That Influence Cherukuri PF, Wincovitch S, Anderson SM; 10.1016/j.bpj.2011.10.050. Epub 2011 Dec 20. Breastmilk and a Tale from West Virginia. NISC Comparative Sequencing Center, Breastfeeding Medicine. 2011 June; 6(3):109- Mullikin JC, Gallagher PG, Hardison RC, Hopewell B, Steiner LA, Bizzarro MJ, 110. Margulies EH, Bodine DM. Genome-wide Ehrenkranz RA, Gallagher PG. Partial ChIP- Seq reveals a dramatic shift in the Exchange Transfusion for Polycythemia Lawrence RA. Disasters at Home and binding of the transcription factor erythroid Hyperviscosity Syndrome. American Journal Abroad. Breastfeeding Medicine. 2011 April; Kruppel-like factor during erythroid of Perinatology. 2011 Aug; 28(7):557-64. 6(2)2:53. differentiation. Blood. 2011 Oct 27; Epub 2011 Mar 1. 118(17):e139-48. Lawrence RA. The Obstetrician as the Hwang J-W, Rajendrasozhan S, Yao H, Chung Lynchpin to Successful Breastfeeding. Popova AP, Bentley J, Bozyk P, Goldsmith A, S, Sundar IK, Huyck HL, Pryhuber GS, Breastfeeding Medicine. 2011 Feb; 6(1):1-2. Linn M, Lei J, Pryhuber G, Hershenson M. Kinnula VL, Rahman I. FoxO3 deficiency Glycogen Synthase Kinase-3beta/beta-Catenin Lawrence RA, Howard CR. Final thoughts leads to increased susceptibility to cigarette Signaling in Neonatal Lung Mesenchymal from the chairs. Breastfeeding Medicine. 2011. smoke-induced inflammation, airspace Stromal Cell Myogibroblastic Differentiation. (6)359-60. enlargement, and chronic obstructive Am J Respir Crit Care Med 2011; 183:A2509.

Golisano Children’s Hospital 2013 – 2014 Annual Report Pressman EK, Thornburg LL, Glantz JC, Stoll BJ, Guillet R, Finer NN, Stevenson DK, Yee M, White RJ, Awad HA, Bates WA, Earhart A, Wall PD, Ashraf M, Pryhuber G, Bauer CR; for the Eunice Kennedy Shriver McGrath-Morrow SA, O’Reilly MA. Neonatal Woods JR. Inflammatory Cytokines and National Institute of Child Health and Human Hyperoxia causes pulmonary Antioxidants in Midtrimester Amniotic Fluid: Development Neonatal Research Network. and shortens life-span in aging mice. Am J Correlation with Pregnancy Outcome. Am J Predictive Value of an Early Amplitude Pathol. 2011; 178:2601-2610. Ob Gyn 2011; 204(2):155 e1-7. Integrated Electroencephalogram and Neurological Examination. Pediatrics. 2011 2012 Raghavendran KR, Willson D, Notter RH. Jul; 128(1):e112-e120. Surfactant therapy of ALI/ARDS. Crit Care Amin SB, Scholer L, Srivastava M. Pre- Clin 2011. Steiner LA, Schulz V, Maksimova Y, Wong discharge iron status and its determinants in C, Gallagher PG. Patterns of Histone H3 premature infants. J Matern Fetal Neonatal Rahman A, Fazal F. Blocking NF-κB: An Lysine 27 Monomethylation and Erythroid Med. 2012 Nov;25(11):2265-9. Inflammatory Issue. Proceedings of the Cell-Type Specific Gene Expression. J Biol American Thoracic Society. 2011 Nov; Chem. 2011 Nov 11; 286(45):39457-65. Epub Amin SB, Orlando M. Optimum Click Rate 8(6):497-503. 2011 Sep 21 for Neurodevelopmental Evaluation Using Auditory Brainstem Response in Premature Scheible K, Zhang G, Baer J, Azadniv M, Steiner LA, Ehrenkranz RA, Peterec S, Infants. Am J Perinatol. 2012 Sep;29(8):587- Lambert K, Pryhuber G, Treanor JJ, Topham Steiner RD, Reyes-Múgica M, Gallagher P. 92. DJ. CD8+T cell immunity to 2009 pandemic Perinatal Onset Mevalonate Kinase and seasonal H1N1 influenza viruses. Vaccin. Deficiency. Pediatr Dev Pathol. 2011 Jul-Aug; Amin SB, Myers G, Wang H. Association 2011 Mar; 3:29(11): 2159-2168. PMCID: 14(4):301-6. doi: 10.2350/11-02-0985-OA.1. between neonatal iron overload and early 3061835. Epub 2011 Mar 22. human brain development in premature infants. Early Human Development. 2012 Schor N, Guillet R, McAnarney E. The Early Treatment for Retinopathy of 88(8)583-7. Anticipatory guidance as a principle of faculty Prematurity Cooperative Group. (Phelps DL development: managing transition and change. PI of a participating center and on the ETROP Amin SB, Scholer L, Srivastava M. Pre- Acad Med. 2011 Oct; 86(1):1235-40. Executive Committee) Visual Field Extend at discharge iron status and its determinants in Age 6 Years in Children who had High-Risk premature infants. J Maternal Fetal Neonatal Schulman J, Stricof R, Stevens TP, Horgan M, Prethreshold Retinopathy of Prematurity. Arch Med. 2012. 25(11)2265-9. Gase K, Holzman IR, et al. Statewide NICU Ophthalmol 2011; 129:127-132. central line- associated bloodstream infection Amin SB, Burnell E. Monitoring Apnea of rates decline after bundles and checklists. Wheeler DT, Dobson V, Chiang MF, Bremer Prematurity: Validity of Nursing Pediatrics. 2011 Mar; 127(3):436-444. DL, Gewolb IH, Phelps DL, Hardy RJ, Good Documentation and Bedside Cardiorespiratory WV, Fellows R, Tung B, Palmer EA. Monitor. Am J. Perinatol. 2013 Sep;30(8):643- Schwan AL, Singh SP, Davy JA, Waring AJ, Retinopathy of Prematurity in Infants 8. doi: 10.1055/s-0032-1329694. Epub 2012 Gordon LM, Walther FJ, Wang Z, Notter RH: Weighing Less Than 500 Grams at Birth Dec 19. Synthesis and activity of a novel diether Enrolled in the Early Treatment for phosphonoglycerol in phospholipase-resistant Retinopathy of Prematurity (ETROP) Study. Bhattacharya S, Go D, Krenitsky D, Huyck synthetic lipid:peptide lung surfactants. Med Ophthalmology. 2011; Jan, E1-E7. H, Solleti S, Lunger V, Metlay L, Srisuma S, Chem Commun, 2:1167-1173 2011. Wert S, Mariani TJ, Pryhuber G. Genome- Wilson KM, Finkelstein JN, et al. Wide Transcriptional Profiling Reveals Shankaran S, Pappas A, McDonald SA, Micronutrient levels in children exposed to Connective Tissue Mast Cell Accumulation in Laptook AR, Bara R, Ehrenkranz RA, Tyson secondhand tobacco smoke. Nicotine & Bronchopulmonary Dysplasia. Am J Respir JE, Goldberg R, Donovan EF, Fanaroff AA, Tobacco Research. 2011; 13(9) 800-8. Crit Care Med. 2012. Aug 15;186(4):349-58. Das A, Poole WK, Walsh M, Higgins RD, Welsh C, Salhab W, Carlo WA, Poindexter B, Wilson DF, Notter RH. New Horizons: The Bijli KM, Fazal F, Rahman A. Regulation of Stoll BJ, Guillet R, Riner NN, Stevenson DK, future of exogenous surfactant therapy. J RelA/65 and endothelial cell inflammation by Bauer CR for the Eunice Kennedy Shriver Respir Care. 2011; 56:1369-1386. prolinerich tyrosine kinase 2. Am J Respir Cell NICHD Neonatal Research Network. Neonatal Mol Biol. 2012.47:660-668. Hypoxic Ischemic Encephalopathy: Predictive Wu YCM, O’Reilly MA. Bcl-XL is the value of an Early Amplitude Integrated EEG primary mediator of p21 protection against Buczynski BW, Yee M, Lawrence BP, and Neurological Examination. Pediatrics. hyperoxia-induced cell death. Exp. Lung Res. O’Reilly MA. Lung development and the host 2011 Jul; 128(1):e112-20. 2011; 37: 82-91. response to influenza A virus are altered by different doses of neonatal oxygen in mice. Shankaran S, Pappas A, McDonald SA, Yang L, Chen G, Mohanty S, Scott G, Fazal Am J Physiol Lung Cell Mol Physiol. 2012 Laptook AR, Bara R, Ehrenkranz RA, Tyson F, Rahman A, Begum S, Hynes R, Xu L. May 15; 302(10):L1078-87. JE, Goldberg R, Donovan EF, Fanaroff AA, GPR56 regulates VEGF production and Das A, Poole WK, Walsh M, Higgins RD, angiogenesis during melanoma progression. Butler-O’Hara M; D’Angio CT, Hoey H, Welsh C, Salhab W, Carlo WA, Poindexter B, Cancer Res. 2011; 71:5558-5568. Stevens TP. An evidence-based catheter

Golisano Children’s Hospital 2013 – 2014 Annual Report bundle alters central venous catheter strategy Gupta K, Amin SB. Case 1008: Management Shakaran S, Pappas A, McDonald S, Vohr B, in newborn infants. J Pediatr. 2012; of Neonatal Jaundice. Peri-Facts. September Hintz S, Yolton K, Gustafson K, Leach T, 160(6):972-7.e2. 2012. Green C, Bara R, Petrie Huitema C, Ehrenkranz R, Tyson J, Das A, Hammon J, Brownell EA, Howard CR, Lawrence RA, Han X, Corson N, Wade-Mercer P, Peralta-Carcelen M, Evans P, Heyne R, Dozier AM. Delayed onset lactogenesis II Finkelstein N, et al. Assessing the relevance Wilson-Costello D, Vaucher Y, Bauer C, predicts the cessation of any or exclusive of in vitro studies in nanotoxicology by Dusick A, Adams-Chapman, Goldstein R, breastfeeding. Journal of Pediatrics. 2012 examining correlations between in vitro and in Guillet R, for the Eunice Kennedy Shriver Oct;161(4):608-14. vivo data. Toxicology. 2012. 297(1-3) 1-9. NICHD Neonatal Research Network. Childhood outcomes after hypothermia for Brownell EA, Howard CR, Lawrence RA, Hoffmire CA; Chess PR; Ben Saad T; Glantz neonatal encephalopathy. NEJM 366:2085- Dozier AM. The effects of immediate JC. Elective Delivery before 39 Weeks: The 2092. 2012. postpartum depot medroxyprogesterone on Risk of Infant Admission to the Neonatal early breastfeeding cessation. Contraception. Intensive Care Unit, Maternal and Child Scheible K, Secor-Socha S, Wightman T, 2012. 87(6): 836-43. Health Journal, 16: 1053-1062. 2012. Wang H, Mariani T, Topham DJ, Pryhuber G, Quataert S. Stability of T cell phenotype Brownell EA, Howard CR, Lawrence RA, Lasky RE, Church MW, Orlando MS, Morris and functional assays following heparinized Dozier AR. Does delayed onset lactogenesis II BH, Parikh NA, Tyson JE, McDavid GE, Oh umbilical cord blood collection. Cytometry A. predict the cessation of any or exclusive W, Stevenson DK, Van Meurs KP, Guillet R, 2012 Nov; 81A(11):937-949. breastfeeding? Journal of Pediatrics. 2012. Phelps DL. Effects of Aggressive Versus 161(4)608-43. Conservative Phototherapy on the Brainstem Shankaran S, Pappas A, McDonald SA, Vohr, Auditory Evoked Responses of Extremely Hintz SR, Yolton K, Guillet R, et al. for the Bozyk PD, Bentley JK, Popova AP, Anyanwu Low Birth Weight Infants. Peds Research. Eunice Kennedy Shriver NICHD Neonatal AC, Pryhuber GS. Neonatal periostin 2012; 71(8):77-84. Research Network. Childhood outcomes after knockout mice are protected from hyperoxia- hypothermia for Neonatal Encephalopathy. induced alveolar simplication. PloS One. 2012; Lawrence RA. Breastfeeding-a public health NEJM 366:2085-2092. 2012. 7(2):e331336. issue, not just a matter of choice. Breastfeeding Medicine. 2012. 7(2)67-8. Shankaran S, Pappas A, McDonald S, Vohr B, Carroll KN, Griffin MR, Edwards KM, Ali A, Guillet R, et al. Childhood outcomes after Zhu Y, Iwane M, Szilagyi P, Staat M, Stevens Lawrence RA. Challenges to successful hypothermia for neonatal encephalopathy. T, Hall C, Williams J, Hartert T. Adherence to breastfeeding. Breastfeeding Medicine. 2012; NEJM. 2012 May 31; 366(22): 2085-92. guidelines for respiratory syncytial virus 7(1)1-2. immunoprophylaxis among infants with Stevens TP, Schulman J. Evidence-based prematurity or chronic lung disease in three O’Reilly MA, Yee M, Buczynski BW, Vitello approach to preventing central line-associated United States counties. The Pediatric PF, Keng PC, Welle SL, Finkelstein JN, bloodstream infection in the NICU. Acta Infectious Disease Journal. 2012 Epub 2012 Dean DA, Lawrence BP. Neonatal oxygen paediatrica (Oslo, Norway: 1992), Supplement Jul 3. increases sensitivity to influenza a virus 2012; 101(464): 11-6. infection in adult mice by suppressing Chess PR, Nayrouz M. Prenatal epithelial expression of ear1. Am J Pathol. Tyson JE, Pedroza C, Langer J, Green C, Glucocorticoid treatment and effect on lung 2012 Aug; 181(2):441-51. Morris B, Stevenson D, Van Meurs KP, Oh W, development. Peri-Facts 978: 1-6. 2012 Phelps D, O'Shea M, McDavid GE, Grisby C, O’Reilly MA. Angiotensin II: tapping the cell Higgins R, for the Eunice Kennedy Shriver Giannandrea M, Yee M, O’Reilly M, cycle machinery to kill endothelial cells. Am J NICHD Neonatal Research Network. Does Lawrence B. Memory CD8+ T cells are Physiol Lung Cell Mol Physiol. 2012; 303: aggressive phototherapy increase mortality sufficient to alleviate impaired host resistance L575-L576. while decreasing profound impairment among to influenza A virus infection caused by the smallest and sickest newborns? Should the Popova AP, Bentley JK, Anyanwau AC, neonatal oxygen supplementation. Clinical and smallest & sickest newborns be treated with Richardson MN, Linn MJ, Lei J, Wong EJ, Vaccine Immunology. 19:1432-1441. 2012 aggressive phototherapy. Journal of Pryhuber G, et al. Am J Physiol Lung Cell Perinatology. Sep;32(9):677-84. doi: Guillet R, Edwards AD, Thorsen M, Ferriero Mol Physiol. 2012 June 21 [epub ahead of 10.1038/jp.2012.64. Epub 2012 May 31. 2012. DM, Gluckman PD, Whitelaw A, Gunn AJ; print] CoolCap Trial Group. Seven-to-eight-year The Early Treatment for Retinopathy of Roy SB, Sadowitz P, Andrews L, Gatto W, follow-up of the CoolCap trial of head cooling Prematurity Cooperative Group. (Phelps DL Dean DA, et al. Early stabilizing alveolar for neonatal encephalopathy. 2012 Pediatr Res. PI of a participating center and on the ETROP ventilation prevents ARDS – A novel timing Feb; 71(2): 205:9. Executive Committee) Grating Visual Acuity based ventilator intervention to avert lung Results in the Early Treatment for Retinopathy Gupta K, Amin SB. Case 1006: Physiology of injury. J Trauma Acute Care Surg. 2012; of Prematurity Study. Arch Ophthalmol. 2012 Neonatal Jaundice. Peri-Facts. September 73:391-400. Jun;130(6):719. 2012.

Golisano Children’s Hospital 2013 – 2014 Annual Report Wynn JL, Li LI, Cotton CM, Goldberg RN, Guillet R. Interdisciplinary simulation-based Miller JH, Reed CK, Lawrence BP, Williams Stoll BJ, D’Angio CT, for the PCV7 training to improve delivery room JP, Finkelstein JN. Exacerbation of lung Subcommittee of the NICHD Neonatal communication. Simul Healthc 2013;8(5):279- radiation injury by viral infection: The role of Research Network (NRN). The Effect of 91. Clara cells and Clara cell secretory protein. Sepsis on Subsequent PCV7 Vaccine Radiation Research. 2013 Jun; 179(6):617-29. Responses in Very Low Birth Weight Infants. Dean D. Cell-specific targeting strategies for Pediatric Academic Societies. 2012; 3415A.2. electroporation-mediated gene delivery in cells Manning C, Johnston C, Reed C, Lawrence and animals. J Membr Biol. Octo: 246(1):737- B, Williams J, Finkelstein J. Lung irradiation York JM, McDaniel AW, Blevins N, Guillet 44. 2013. increases mortality following influenza a virus R, et al. Individually ventilated cages cause challenge occurring late after exposure. chronic low-grade hypoxia impacting mice Dozier AM, Howard CR, Brownell EA, International Journal of Radiation hematologically and behaviorally. Brain Behav Lawrence RA. Labor epidural anesthesia, Oncology*Biology*Physics. 2013; 86(1):128- Immun. 2012 Aug; 26(6):951-8. obstetric factors and breastfeeding cessation. 135. Maternal and Child Health Journal. 2013. 2013 17(4)689-14. Mariani TJ, Martinez F. Chronic obstructive pulmonary disease genomics: yesterday, Allen, JA Conrad K, Oberdorster G, Johnston Emr B, Gatto L, Roy S, Satalin J, Ghosh A, discovering population biomarkers; tomorrow, CJ, Sleezer B, Cory-Slecta DA. Snyder K, Andrews P, Habashi N, Marx W, defining disease clusters. American Journal of Developmental exposure to concentrated Ge L, Wang G, Dean D, Vodovotz Y, Nieman Respiratory and Critical Care Medicine. 2013. ambient particles and preference for immediate G. Airway pressure release ventilation 187(9): 900-2. reward in mice. Env Health Perspect. 2013. prevents ventilator-induced lung injury in 121(1):32-38. normal lungs. JAMA Surg. 2013. Nov; Meyers JM, Greecher CP, Shaffer ML, 148(11):1005-12. Shenberger JS. Potential influence of total Amin SB, Orlando M, Wang H. Latent iron parenteral nutrition on body composition at discharge in preterm infants. J Matern Fetal deficiency in utero is associated with abnormal Fazal F, Bijli KM, Murrill M, Leonard A, Minhajuddin M, Anwar KN, Finkelstein JN, Neonatal Med. 2013 Oct;26(15):1548-53. doi: auditory neural myelination in >/=35 weeks 10.3109/14767058.2013.793663. Epub 2013 Watterson DM, Rahman A. Critical role of gestational age infants. J Pediatr. 2013; May 7. 163(5):1267-71. non-muscle myosin light chain kinase in thrombin-induced endothelial cell Morris BH, Tyson JE, Stevenson DK, Oh W, Badding MA, Lapek JD, Friedman AE, Dean inflammation and lung PMN recruitment. Phelps DL, O’Shea TM, McDavid GE, DA. Proteomic and functional analyses of PLoS ONE 8:e59965. 2013. VanMeurs KP, Vohr BR, Grisby C, Yao Q, protein-DNA complexes during gene transfer. Taylor S, Wallace D, Higgins RD. Efficacy of Mol Ther. 2013 Apr;21(4):775-85. doi: Finkelstein JN. Exacerbation of Lung phototherapy devices and outcomes among 10.1038/mt.2012.231. Epub 2012 Nov 20. Radiation Injury by Viral Infection: The Role extremely low birth weight infants: multi- of Clara Cells and Clara Cell Secretory Bhattacharya S, Mariani TJ. Systems center observational study. J Perinatol. 2013 Protein. Radiation Research. 2013. Epub 2013 biology approaches to identify developmental Feb; 33(2):126-33. April 26. bases for lung disease. Pediatric Research. Morrisey EE, Cardosos WV, Lane RH, 2013 Apr; 73(4 Pt 2):514-22. Johnston CJ, Manning CM, Rangel-Moreno Mariani TJ, et al. Molecular determinants of J, Randall TD, Hernady E, Finkelstein JN, Buczynski BW, Madwueke E, O’Reilly MA. lung development. Ann Am Thorac Soc. 2013; Williams JP. Neonatal Irradiation Sensitizes The role of hyperoxia in the pathogenesis of 10(2):S12-6. Mice to Delayed Pulmonary Challenge. experimental BPD. Semin. Perinatol. 2013 Radiation Research. 2013. 179(4):475-484. Roy S, Habashi N, Sadowitz B, Andrews P, Apr;37(2):69-78. Ge L, Wang G, Roy P, Ghosh A, Kuhn M, Machado-Aranda D, Wang Z, Yu B, Suresh Chess PR, Malhotra Y, Laroia N. Neonatal Satalin J, Gatto L, Lin X, Dean D, Vodovotz MV, Notter RH, Raghavendran K. Increased Care. In Guerrero C (ed). Nancy Caroline’s y. Early airway pressure release ventilation phospholipase A2 and lyso- Emergency Care in the Streets, Seventh prevents ARDS-a novel preventive approach phosphatidylcholine levels are associated with edition. Burlington, MA: Jones and Bartlett. to lung injury. Shock. Jan; 39(1):28-38. 2013 surfactant dysfunction in lung contusion injury 874-921. 2013. in mice. . 2013 Jan;153(1):25-35. Thach CT, Finkelstein JN. Cationic Choo-Wing R; Syed M, Harijith A, Bowen B, nanoparticles disrupt cellular signaling in a Maniscalco WM, Bhandari, V. Disruption in Pryhuber G, et al. Hyperoxia and Interferon-g cholesterol dependent manner. Toxicology in lung vascular development. In: Abman, S (ed). Induced Injury in Developing Lungs Occur via vitro: an international journal published in BPD. New Mortality Following Influenza A Cox2 and the ER Stress Dependent Pathway. association with BIBRA. 2013. 27(4) 1277-86. Virus Challenge Occurring Late After Am J Resp Cell and Mol Biol. 2013 Exposure. 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Golisano Children’s Hospital 2013 – 2014 Annual Report stream infection is associated with altered Westerman M, O’Brien K. Placental heme Adolescent Nutrition. 2014. In Press. heptavalent pneumococcal conjugate vaccine receptor LRP1 correlates with the heme immune responses in very low birth weight exporter FLVCR1 and neonatal iron status. Lumish R, Young S, Lee S, Cooper E, infants. J Perinatol. 2013 Jan 31. PMID: Reproduction. 2014 June 19 Pressman E, Guillet R, O’Brien K. Gestational 23370608. iron deficiency is associated with pica Cotton C, Goldstein R, McDonald S, Goldberg behaviors in adolescents. J Nutr. 2014 Oct; Yee M, Buczynski BW, Lawrence BP, R, Salhab W, Carlo W, Tyson J, Finer N, 144(10): 1524-32. O’Reilly MA. Neonatal hyperoxia increases Walsh M, Ehrenkranz R, Laptook A, Guillet sensitivity of adult mice to bleomycin-induced R, et al. Eunice Kennedy Shriver National Qiu W, Rogers A, Damask A, Raby B, lung fibrosis. Am J Respir Cell Mol Biol. Institute of Child Health and Human Klanderman B, Duan Q, Tyagi S, Niu S, 2013; 48:258-266. Development Neonatal Research Network. Anderson C, Cahir-McFarland E, Mariani T. Apolipoprotein E genotype and outcome in Pharmacogenomics: Novel Loci Identification 2014 infants with hypoxic-ischemic encephalopathy. via Integrating Analysis and eQTL Analysis. Pediatr Res. 75(3):424-30, 2014. Hum Mol Genet. 2014 Apr 25 [Epub ahead of Allen J, Liu X, Weston D, Prince L, print] Oberdorster G, Finkelstein J, Johnston C, Dadiz R, Weinschreider J, Pressman E, Cory-Slecta D. Developmental exposure to Guillet R. Delivery room communication Young J, Varravecchia M, Dean D. concentrated ambient ultrafine particulate checklist. MedEdPORTAL 2014. Electroporation-mediated gene delivery to the matter air pollution in mice results in persistent lungs. Methods Mol Biol 2014; 1121:189-204. and sex-dependent behavioral neurotoxicity Dardas M, Gill S, Grier A, Pryhuber G, Gill and glial activation. Toxicol Sci 2014; A, Lee Y, Guillet R. The impact of postnatal antibiotics on the preterm intestinal 140(1):160-178. microbiome. Pediatra Res. 2014 Aug; Bello-Irizarry S, Wang J, Johnston C, 76(2):150-8. Gigliotti F, Wright T. MyD88 signaling regulates both host defense and DeMauro S, D’Agostino J, Bann C, Bernbaum immunopathogenesis during Pneumocystis J, Gerdes M, Bell E, Carlo W, D’Angio C, infection. J Immunol. 2014; 192(1):282-292. Das A, Higgins R, Hintz Sr, et al. Eunice Kennedy Shriver NICHD Neonatal Research

Bijli KM, Kanter BG, Minhajuddin M, Network. Developmental outcomes of very Leonard A, Xu L, Fazal F, Rahman A. preterm infants with tracheostomies. J Pediatr. Regulation of endothelial cell inflammation 2014; 164(6):1303-10. and lung PMN infiltration by transglutaminase 2. Shock 2014 Emr B, Roy S, Kollisch-Singule M, Gatto L, Barravecchia M, Lin X, Young J, Wang G, Boghossian N, Hansen N, Bell E, Stoll B, Satalin J, Snyder K, Nieman G, Dean D.

Murray J, Carey J, Adams-Chapman I, Electroporation mediated gene delivery of Shankaran S, Walsh M, Laptook A, Faix R, Na+, K+-ATPase and ENaC Subunits to the Newman N, D’Angio C, et al. Eunice Lung Attenuates Acute Respiratory Distress Kennedy Shriver National Institute of Child Syndrome in a Two-Hit Porcine Model. Health and Human Development Neonatal Shock. 2014. In Press.

Research Network. Mortality and morbidity of Lee S, Guillet R, Cooper E, Westerman M, VLBW infants with trisomy 13 or trisomy 18. Pediatrics. 2014 Feb; 133(2):226-35. Orlando M, Pressman E, O’Brien K. Maternal inflammation at delivery affects assessment of Butler-O’Hara M, Reininger A, Dadiz R. maternal iron status. J Nutr. 2014 Oct; Training in placement of peripherally inserted 144(10):1524-32. central catheters in the neonate.

MedEdPORTAL 2014. Lee S, Essley B, Cooper E, Pressman E, Queenan R, McIntyre A, Guillet R, O’Brien Cao C, Pressman E, Cooper E, Guillet R, K. Pregnant adolescents consume diets deficient in calcium and iron. Infant Child and

Golisano Children’s Hospital 2013 – 2014 Annual Report