TRPC1 Antibody Affinity Purified Polyclonal Antibody Catalog # AG1458

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TRPC1 Antibody Affinity purified polyclonal antibody Catalog # AG1458

Specification

TRPC1 Antibody - Product Information

Application WB, IHC Primary Accession P48995 Reactivity Human, Mouse, Rat Host Rabbit Clonality Polyclonal Calculated MW 91212 Homology Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Western blot analysis of rat brain Xenopus Laevis - membranes: 11/14 amino acid 1. Anti-TRPC1 antibody (#AG1458), (1:200). residues identical. 2. Anti-TRPC1 antibody, preincubated with the control peptide antigen.

TRPC1 Antibody - Additional Information

Gene ID 7220

Other Names Short transient receptor potential channel 1, TrpC1, Transient receptor protein 1, TRP-1, TRPC1, TRP1

Related products for control experiments Control peptide antigen (supplied with the antibody free of charge).

Target/Specificity Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human .TRPC1 (Accession P48995).ֲ ֲ Intracellular Expression of TRPC1 in mouse cerebellum Immunohistochemical staining of mouse Dilution WB~~1:200-1:2000 cerebellum frozen sections using Anti-TRPC1 antibody (#AG1458). A. TRPC1 (red) appears in Peptide Confirmation Purkinje cells (arrows) and in the molecular Confirmed by amino acid analysis and (Mol) and granule (Gran) layers. B. Staining massspectrography. with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC1 and Application Details PV demonstrates partial co-localization in the Western blot analysis (WB): - Mouse brain Purkinje and the molecular layers. lysate (see Feng, S. et al. (2013) in Product Citations). - Rat distal pulmonary smooth muscle cell lysate (PASMCs), (see TRPC1 Antibody - Background Zhang, Y. et al. (2013) in Product Citations). Immunoprecipitation (IP): The Transient Receptor Potential (TRP) - Human umbilical vein endothelial cells superfamily is one of the largest ion channel (HUVECs), (see Ahmmed, G.U. et al. (2004) in Product Citations). families and consists of diverse groups of Immunocytochemistry (IC): - BAE-1 (Bovine proteins. In mammals about 28 genes encode aortic endothelium) cells (1:200) (see the TRP ion channel subunits. The mammalian Antoniotti, S. et al. (2002) in TRP superfamily comprises six subfamilies known Product Citations). - Human U373 MG cells as the TRPC (canonical), TRPV (vanilloid), TRPM (1:80) (see Barajas, M. et (melastatin), TRPML (mucolipins), TRPP al. (2008) in Product Citations). (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of Format seven proteins named TRPC1 to 7, which can be Affinity purified antibody, lyophilized powder further divided into four subgroups based on their sequence homology and functional Reconstitution similarities: 1) TRPC1 2) TRPC4 and TRPC5 3) 25 µl, 50 µl or 0.2 ml deionized water, TRPC3, TRPC6, TRPC7 4) TRPC2.2,5 They are depending on the sample size. highly expressed in the central nervous system Antibody Concentration After and to a lesser extent in peripheral tissues. Reconstitution TRPC1 was the first mammalian TRP protein that 0.8 mg/ml. was reported to form an ion channel.2 It can co-assemble with other TRPC subunits (TRPC3, Buffer After Reconstitution TRPC4, TRPC5) to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 1-3 Storage Before Reconstitution homomers has not been established as yet. Lyophilized powder can be stored intact at The TRPC1, TRPC4 and TRPC5 can be activated room temperature for several weeks. For either by Ca2+ store depletion or by GPCR longer periods, it should be stored at -20°C. stimulation pathways, while TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that Storage After Reconstitution are activated by the stimulation of GPCRs. The reconstituted solution can be stored at TRPC1, 4 and TRPC5 are assumed to form 4ºC for up to 2 weeks. For longer periods, components of store operated channels in some small aliquots should be stored at -20ºC or cell types such as salivary gland cells, below. Avoid multiple freezing and thawing. endothelial cells and vascular smooth muscle The further dilutions should be made using a cells.6 carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 TRPC1 Antibody - References × g 5 min). References 1. Moran, M.M. et al. (2004) Current Control Antigen Storage Before Reconstitution Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. Lyophilized powder can be stored intact at (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. room temperature for several weeks. For (2003) Nature 426, 517. 4. Padinjat, R. and longer periods, it should be stored at -20°C. Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. Control Antigen Reconstitution 6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337. 100 µl water.

Control Antigen Storage After Reconstitution -20ºC.

Preadsorption Control 1 µg peptide per 1 µg antibody.

Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

TRPC1 Antibody - Protocols

Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture