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The complete mitochondrial genome of Picus canus (: Picidae)

Ji-Yuan Yao, Wei-Ning Lai, Bo Li, Ming-Yue Hu, Tian-Qi Fan, Fu-Guang Ma & Shou-Qing Yan

To cite this article: Ji-Yuan Yao, Wei-Ning Lai, Bo Li, Ming-Yue Hu, Tian-Qi Fan, Fu-Guang Ma & Shou-Qing Yan (2019) The complete mitochondrial genome of Picus￿canus (Piciformes: Picidae), Mitochondrial DNA Part B, 4:1, 1869-1870, DOI: 10.1080/23802359.2019.1614887 To link to this article: https://doi.org/10.1080/23802359.2019.1614887

© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Published online: 11 May 2019.

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MITOGENOME ANNOUNCEMENT The complete mitochondrial genome of Picus canus (Piciformes: Picidae)

Ji-Yuan Yaoa, Wei-Ning Laib,BoLia, Ming-Yue Hub, Tian-Qi Fana, Fu-Guang Maa and Shou-Qing Yanb aAnimal Science and Technology College, Jilin Agricultural University, Changchun, P.R. China; bCollege of Science, Jilin University, Changchun, P.R. China

ABSTRACT ARTICLE HISTORY The grey-headed (Picus canus) is one species of family Picidae. In this study, the complete Received 4 January 2019 mitochondrial genome of the grey-headed woodpecker was determined and annotated for the first Accepted 3 February 2019 time. It is a circular molecule of 17,103 bp in size and contains 13 protein-coding genes (PCGs), 2 rRNA KEYWORDS genes (12S and 16S), 22 tRNA genes (tRNAs), and 2 control regions (D-loop regions). The total base Grey-headed woodpecker; composition is 28.13% for A, 34.57% for C, 13.87% for G, and 23.43% for T, respectively. In addition, mitochondrion; genome phylogenetic analysis was carried out according to the DNA data of 13 PCGs using the neighbour- joining method. The complete mitogenome of grey-headed woodpecker will play an important role in population genetics and phylogenetic studies of the species in the future.

The mitochondrial genome in mammals is a circular and dou- Genomic DNA from muscle tissue of an adult grey-headed ble-stranded DNA molecule with approximately 14.5–19.5 kb woodpecker was extracted using the EasyPure Genomic DNA in size and codes for 37 genes (Du Toit et al. 2014). The DNA Kit (Transgen, Beijing, China). The sample was collected from sequences from mitochondrial genome have played import- Changchun City of Jilin Province in China (4348035.37ʺN and ant roles in studies related to phylogenetic relation, genetic 1252409.58ʺE) and stored in Zoological Specimen Museum of diversity, and evolution due to its characteristics of maternal Jilin Agricultural University. The mitochondrial genome was inheritance, fast mutation rate, and high copy number (Wada amplified with 12 pairs of primers designed according to the et al. 2010; Anmarkrud and Lifjeld 2017;Suarez-Villota et al. known DNA sequences of the grey-headed woodpecker and 2018). The grey-headed woodpecker (Picus canus), also Dendrocopos major. The PCR product including the control known as the grey-faced woodpecker, is one species of fam- region 1 was cloned into a pGM-T vector (TIANGEN, Beijing, ily Picidae including about 183 species in 24 genera (Benz China) and then sequenced. All other products were purified et al. 2006). To date, only partial sequence of the mitochon- and directly sequenced. drial DNA of grey-headed woodpecker is available. In the pre- The size of the mitochondrial genome for the grey-headed sent study, the full-length mitochondrial genome DNA for woodpecker is 17,103 bp and include 13 protein-coding genes the grey-headed woodpecker was sequenced and annotated (PCGs), 22 transfer tRNAs (tRNAs), 2 ribosomal RNA genes (12S successfully. and 16S), and 2 control regions (D-loop regions). The gene

Figure 1. The neighbour-joining (NJ) tree of 9 species was constructed based on the data set of 13 concatenated mitochondrial PCGs using MEGA 5 with 1000 bootstrap replicates. Sequence data used in this study are the following: pubescens (KT119343), Dendrocopos leucotos (KU131555), Dendrocopos major (KT350609), Campephilus guatemalensis (KT443920), Campephilus imperialis (KU158198), Sasia ochracea (KT443919), Dryocopus pileatus (DQ780879) and Pteroglossus azara flavirostris (DQ780882).

CONTACT Ji-Yuan Yao [email protected] Animal Science and Technology College, Jilin Agricultural University, Changchun 130118, P.R. China ß 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 1870 J.-Y. YAO ET AL. order was identical to that found in other species of family References Picidae. The base composition is 28.13% for A, 34.57% for C, Anmarkrud JA, Lifjeld JT. 2017. Complete mitochondrial genomes of 13.87% for G and 23.43% for T, respectively. Except for ND6 eleven extinct or possibly extinct species. Mol Ecol Resour. 17: and 8 tRNA genes, other genes are coded on the H strand. 334–341. The mitochondrial genome of grey-headed woodpecker was Benz BW, Robbins MB, Peterson AT. 2006. Evolutionary history of wood- submitted to GenBank (accession no. MK348064). peckers and allies (Aves: Picidae): placing key taxa on the phylogen- Phylogenetic analysis was carried out according to the etic tree. Mol Phylogenet Evol. 40:389–399. DNA data of 13 PCGs of the grey-headed woodpecker and Du Toit Z, Grobler JP, Kotze A, Jansen R, Brettschneider H, Dalton other 8 Piciformes species. The neighbour-joining (NJ) tree DL. 2014. The complete mitochondrial genome of temminck’s ground pangolin (Smutsia temminckii; Smuts, 1832) was constructed using MEGA 5 with 1000 bootstrap repli- and phylogenetic position of the Pholidota (Weber, 1904). Gene. cates (Figure 1). The phylogenetic relationship between the 551:49–54. grey-headed woodpecker and other Picidae species is con- Fuchs J, Pons JM, Ericson PG, Bonillo C, Couloux A, Pasquet E. 2008. sistent with that of the previous study (Fuchs et al. 2008; Molecular support for a rapid cladogenesis of the woodpecker clade Shakya et al. 2017). This mitogenome sequence obtained in Malarpicini, with further insights into the genus Picus (Piciformes: this study will be useful for studying population genetics and Picinae). Mol Phylogenet Evol. 48:34–46. phylogenetic analysis of this species. Shakya SB, Fuchs J, Pons JM, Sheldon FH. 2017. Tapping the woodpecker tree for evolutionary insight. Mol Phylogenet Evol. 116: 182–191. Disclosure statement Suarez-Villota EY, Quercia CA, Nunez~ JJ. 2018. Mitochondrial genomes of the South American frogs Eupsophus vertebralis and E. emiliopugini No potential conflict of interest was reported by the authors. (Neobatrachia: Alsodidae) and their phylogenetic relationships. J Genomics. 6:98–102. Funding Wada K, Okumura K, Nishibori M, Kikkawa Y, Yokohama M. 2010. The complete mitochondrial genome of the domestic red deer (Cervus ela- This study was supported by the Undergraduate Training Program for phus) of New Zealand and its phylogenic position within the family Innovation and Entrepreneurship [No. 201810193027]. Cervidae. Anim Sci J. 81:551–557.