EVALUATION OF BOLDENONE UNDECYLENATE AS AN ANABOLIC AGENT IN HORSES

J. J. O'Connor, M. C. Stillions, W. A. Reynolds, W. H. Linkenheimer, D. C. Maplesden*

INTRODUCTION market. Many of the horses had multiple sad- dle sores and leg lacerations from being trans- THE PARENTERAL ADMINISTRATION of testoster- ported. Fecal samples obtained before the one and other steroids to castrate dogs, rats and animals had been placed on test showed the intact human males has been reported to in- presence of strongyles. All of the horses were crease gains in body weight and nitrogen re- treated with thiabendazole2 as a top dressing tention (3, 5, 6) but in addition to the on the feed. anabolic effects, there were undesirable andro- Throughout the trial, the horses were al- genic side effects. In efforts to find compounds lowed medium quality, mixed hay ad libitum. with improved efficiency and reduced side ef- A concentrate,3 prepared especially for horses, fects, many steroids that possess high anabolic was fed individually to each horse at one-half activity and low androgenic activity have been the recommended level (0.50 kg/ 100 kg BW). synthesized (1, 7) and some are available as The amount of concentrate fed was adjusted anabolic agents to the medical profession. In after each weighing period. veterinary practice an anabolic steroid would The horses were randomly assigned to two be of value in restoring weight losses in ani- groups of five horses each. One group was mals, particularly horses debilitated from over- given a single injection of boldenone unde- work, unthriftiness, or anorexia. The difficul- cylenate in sesame oil (containing 25 mg/ml) ties of conducting adequately controlled stud- at 0.125 mg/kg BW, IM on Day 1 of Week 1. ies with such animals have resulted in many Two weeks later, a second dose of 0.5 mg/kg reports on restoration of weight loss that are BW was administered IM. The other group based on clinical impressions rather than on was given sesame oil IM, at equivalent vol- well-designed experimental trials with ade- umes, as a placebo. quate controls. Body weights were recorded on the day of The present experiments were conducted to initial treatment and every two weeks there- determine the effects in horses of boldenone after. Prior to each weighing the horses were undecylenate (17 ,-hydroxyandrosta-1, 4-dien- deprived of water and feed for approximately 3-one, 17-(10-undecenate)),' a potent, long- 20 hours. Blood samples for hemoglobin4 and acting anabolic steroid. The parameters stud- hematocrit5 determinations were collected from ied were weight gain, nitrogen retention, each horse five days before the initial treat- hemoglobin concentration, hematocrit, and ment and on each weigh day thereafter. All blood serum levels of calcium and phosphorus. data were subjected to analysis of covariance.

MATERIALS AND METHODS Trial 2 Thirty-three horses, 18 geldings and 15 Tests for Gain in Body Weight mares, were purchased at a local livestock Trial 1 market. Data for three of the mares, which To determine the effect of boldenone unde- were pregnant, were subsequently eliminated. cylenate on weight gain, 10 geldings, weak- On arrival they were administered a single 10 ened by heavy work and in a debilitated ml IM injection of procaine penicillin G and condition, were purchased at a local livestock 2Equizoleg - Merck & Co., Inc., Rahway, New *Squibb Agricultural Research Center, Three Jersey 07065. Bridges, New Jersey 08887, and Theracon Inc., 3Agway, Inc., Buffalo, New York 14240. Topeka, Kansas 66601: (Stillions). 4Hb-Meter, American Optical Company, Buf- Present address of M. C. Stillions: Agway Re- falo, New York. 14240. search Laboratories, Ithaca, New York 14850. 5Microhematocrit method, as cited in Veterinary lEquipoise7m - E. R. Squibb & Sons, Inc., P.O. Hematology. 0. W. Schalm, p. 61. Lea & Febiger, Box 4000, Princeton, New Jersey 08540. Philadelphia, 1961. L54 CAN. VET. JOUR., vol. 14, no. 7, July, 1973 BOLDENONE UNDECYLENATE dihydrostreptomycin.6 During an acclimation Protein intake was calculated according to period of approximately four weeks, the horses the National Research Council recommenda- were treated for strongyles using thiabendazole tions (9), so that the diet was neither exces- in the feed. sive nor deficient in protein for growing horses. The horses in this trial were randomly as- The horses were given 3.8 g of protein/kg BW. signed to three groups according to age, Horses were fed twice daily and given water weight and physical appearance. One group ad lib. The urine and feces of the geldings served as a control and received two IM in- were collected while they were in metabolism jections of sesame oil as a placebo. The sec- stalls (10). Each filly was catheterized with ond group was given two IM injections of an 80 ml retention catheter. Total daily urine boldenone undecylenate in sesame oil (con- and fecal collections were made. After each taining 25 mg/ml) at 0.275 mg/kg BW. The was thoroughly mixed, aliquots (urine 10% third group was given two injections of the and feces 20%) were taken for analysis. Nitro- drug in sesame oil (containing 50 mg/ml) at gen was determined by Kjeldahl digestion (2) 1.10 mg/kg BW IM. Injections were given on and colorimetric analysis.l1 After urine and Day 1 of Week 1 of the experiments and again feces had been collected, the horses were exer- two weeks later. Blood samples were taken cised by walking for 25 minutes each day in from the same two horses (one male and one a mechanical exerciser. female in each group) before the injections of After the adjustment period, individual con- drug, on the day of each treatment, and at trol urine and fecal collections were made the end of the study. The sera were analyzed daily for five days. After the collection on the for calcium and phosphorous spectrophoto- sixth day, each of the horses was given an IM metrically.7 injection of boldenone undecylenate, 1.10 mg/ During the first two weeks of treatment the kg BW. Urinary and fecal nitrogen concentra- horses were fed individually at 1.35 kg/100 tions were determined individually on the kg BW divided into two daily feedings. Dur- seventh through 13th days and on composited ing the subsequent two weeks, because of im- samples on Days 14-15, 19-20, and 24-25. On proved appetite, the daily ration was increased Day 26 the horses were again injected with to 1.50 kg/100 kg BW. The ration consisted boldenone undecylenate in the same manner of a concentrate8 and mixed hay of average as described previously. Individual nitrogen quality, fed in equal proportions. concentrations in urine and feces were deter- Body weights were recorded weekly follow- mined on Days 26 through 31 and composited ing overnight deprivation of food and water. on Days 34-35, 38-39 and 43-44. Body weight deviations during the treatment The data were analyzed for statistical sig- period were subjected to analysis of variance nificance by the method of least squares ana- to estimate variation. Dunnett's multiple com- lysis of variance. parison test was used to determine statistical significance of treatment versus control com- parisons. RESULTS Tests for Gain in Body Weight Test for Nitrogen Retention Four geldings and four fillies, ten to 12 Trial 1 months of age and weighing 184 to 284 kg, The horses treated with boldenone unde- were used in this trial. During a 34-day pre- cylenate gained an average of 70.0 kg, com- treatment adjustment period, the animals were pared with 53.8 kg for the placebo-treated first fed hay and a complete pelleted ration.9 group (Table I). The difference was not The hay component of the diet was gradually statistically significant and was attributed to decreased until the horses had been consum- variability among the horses. No differences ing the complete feed for 22 days before col- in hemoglobin and hematocrit values were lections were initiated. found. Trial 2 6Distrycilling - E. R. Squibb & Sons, Inc., P.O. The results of this trial are presented in Box 4000, Princeton, New Jersey 08540. Table II. When data for both sexes were com- 7Clinical manual for the Spectronic 20, Bausch bined the horses treated with boldenone unde- & Lomb, Inc., Rochester, New York 14603. 8Trim, and Choice (50:50 ratio), Agway, Inc., Buffalo, New York 14240. 10Technicon Auto Analyzer - Technicon In- 9Big-um - Ralston Purina Co., St. Louis, Mis- struments Corporation, Tarrytown, New York souri 63155. 10591. 155 CANADIAN VTEIEUNARY JOURNAL

TABLE I EFFECT OF BOLDENONE UNDECYLENATEa ON WEIGHT GAIN IN DEBILITATED HORSESb TRIAL 1 Gain In Mean Body Weight weeks Initial Mean Final Mean Treatment" Body Weight 2 4 6 8 Total Body Weight (kg) ______--kg------(kg) Control 371.4424.5 14.8 17.0 12.8 9.2 53.8±23.7 425.2423.7 Boldenone 349.0437.1 32.6 12.4 18.8 6.2 70.0±29.4 419.0±29.4 undecylenate aEquipoiseThLE. R. Squibb & Sons, Inc., Princeton, New Jersey 08540. bValues are means for five geldings±standard error of the mean OTreatment, 0.125 mg/kg, IM, on day 1 of week 1 and 0.50 mg/kg, IM, 2 weeks later. Drug was in a sesame oil solution containing 25 mg/ml. Controls received equivalent volumes of sesame oil, IM.

TABLE II EFFECT OF BOLDENONE UNDECYLENATEa ON WEIGHT GAIN IN DEBILITATED HORSESb TRIAL 2 Change in Mean Body Weight Weeks Initial Mean Treatment" Dose Body Weight 1 2 3 4 Total mg/kg IM (kg) - - - - kg ------Castrated Males Control - 410.7±32.8 -0.7 -6.7 6.2 -3.5 -4.7 ± 6.0 Boldenone undecylenate 0.275 429.7±48.8 3.5 -9.5 6.0 -1.9 -1.8 ± 4.7 Boldenone undecylenate 1.10 406.8±35.3 3.5 -3.3 5.5 2.2 7.8 ± 12.2 Females Control - 378.7±15.5 1.6 -7.6 4.3 -1.7 -3.3 ± 2.0 Boldenone undecylenate 0.275 415.6±27.0 1.2 -2.4 4.4 0.6 3.8 ± 5.0 Boldenoneundecylenate 1.10 407.5±40.6 1.7 -3.7 3.5 3.0 4.5 + 4.9 Combined Sexes Control - 400.0432.0 0.1 -7.0 5.6 -2.9 -4.22± 5.0 Boldenone undecylenate 0.275 423.3±41.0 2.4 -6.3 5.3 -0.7 0.73i 5.6 Boldenone undecylenate 1.10 407.1±431.9 2.8 -3.5 4.7 2.5 6.50± 10.ld aEquipoiseTm-E. R. Squibb & Sons, Inc., Princeton, New Jersey 08540. bValues are means of 6 geldings and 3, 5 and 4 females per treatment, respectively±standard error of the mean. Unequal distribution of sexes result in slight differences for the combined values. eFirst injection on day 1 of week 1, followed by a similar injection 2 weeks later. Drug was in a sesame oil solution containing 25 mg/ml and 50 mg/ml for the 0.275 mg/kg and the 1.10 mg/kg groups, respectively. Controls received equivalent volumes of sesame oil, IM. dSignificantly different from controls (p <0.05). cylenate, 1.10 mg/kg BW, showed significantly Test for Nitrogen Retention (p<0.05) greater gains in body weight than Nitrogen balance values are given in Table did the horses in the control group. There were III. The data show that horses treated with no significant differences in results between boldenone undecylenate had improved nitro- animals given the higher and lower doses of gen retention. The increase in nitrogen re- boldenone undecylenate or between the con- tained was statistically significant (p

Two trials were conducted with debilitated d'un seuil plus eleve de la retention de l'azote horses. In the first trial, the intramuscular ou d'un "stress". (IM) administration of the drug at 0.125 mg/ kg body weight (BW) and two weeks later at ACKNOWLEDGMENTS 0.50 mg/kg BW resulted in an apparent but The authors thank the following for their assist- non-significant increase in weight gains. Hema- ance: Dr. K. K. Arora, E. R. Squibb & Sons, Inc., tocrit and hemoglobin concentration were not East Brunswick, New Jersey and Mrs. H. Sylwe- affected. In a second trial, two IM injections strowicz, CIBA Pharmaceutical Company, Sum- of boldenone undecylenate at 1.10 mg/kg BW mit, New Jersey. two weeks apart resulted in a significant in- crease in body weight gains. REFERENCES In a nitrogen retention study, two IM in- 1. ALBANESE, A. A., E. J. LoR.EzE, L. A. ORTO jections of this anabolic steroid 19 days apart and I. L. SMULLYAN. Nutritional and meta- at a level of 1.10 mg/kg BW significantly im- bolic effects of some newer steroids. N.Y. proved nitrogen retention in young horses State J. Med. 65: 2116-2125. 1965. after the first injection. Nitrogen retention was 2. A.O.A.C. Official Methods of Analysis (9th not significantly increased after the second in- ed). Association of Official Agricultural due to a higher baseline of Chemists, Washington, D.C. 1961. jection most likely 3. BORUN, E. R. and E. GEIGER. Interrelation- nitrogen retention and stress. ships of some metabolic effects of testosterone propionate in normal males during restricted REsumi sodium intake. J. clin. Invest. 35: 1109-1118. 1956. Les auteurs ont etudie l'effet de l'undecyl6- 4. DONTAS, A. A., J. R. PAPANAYIOTOU and S. nate de boldenone, un steroide anabolique 'a MARKETOS. Renal effects of a new anabolic longue action, sur le gain de poids et la re- steroid (Ba-29, 038) in old age. Pharmacol. Clin. 1: 43-46. 1988. tention de l'azote, chez le cheval. 5. KOCHAKIAN, C. D. and J. R. MURLIN. The Ils effectuerent deux experiences avec des effect of male hormone on protein and energy chevaux malades. Dans la premiere, rinjection metabolism of castrate dogs. J. Nutr. 10: intra-musculaire de ce steroide, 'a raison de 437-458. 1935. 0.125 mg/kg et, deux semaines plus tard, a 6. KOCHAKIAN, C. D. Comparison of protein la dose de 0.50 mg/kg, resulta en un gain de anabolic property of various androgens in the poids apparent, mais peu appreciable. Ces in- castrated rat. Am. J. Physiol. 160: 53-61. jections n'affect&rent pas les valeurs de l'hema- 1950. tocrite ni celles de l'hemoglobine. Au cours de 7. KRiJSKEMPER, H. L. Anabolic Steroids. New intra- York: Academic Press Inc. 1968. la seconde experience, deux injections 8. MITCHELL, H. H. Comparative Nutrition of musculaires du steroide, 'a raison de 1.10 mg/ Man and Domestic Animals. New York: kg, a deux semaines d'intervalle, produisirent Academic Press Inc. 1962. une augmentation appreciable du gain de 9. National Research Council. Nutrient Require- poids. ments of Horses. In Nutrient Requirements Au cours d'une experience sur la retention of Farm Animals. National Research Council, de l'azote, deux injections intra-musculaires de Washington, D.C. 1961. ce st6roide anabolique, 'a 19 jours d'intervalle, 10. STIONS, M. C. and W. E. NELSON. Meta- bolism stall for male equine. J. Anim. Sci. 27: a raison de 1.10 mg/kg, ameliorerent de fawon 68-72. 1968. appreciable la retention de l'azote, chez les 11. STni, H. G. Uber die Anwendung eines jeunes chevaux, apres la premiere injection. anabolen Steroids in der Pferdepraxis. Berl. Aucune augmentation significative de la re- Munch. tierarztl. Wschr. 81: 378-382. 1968. tention de l'azote ne se produisit apres la 12. VIGRE, E. Experience with methandienone. deuxieme injection, vraisemblablement 'a cause Vet. Rec. 75: 769-771. 1963.

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