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1 Supplement Table 1. Freshwater Crayfish Distribution in Greece

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1 Supplement Table 1. Freshwater Crayfish Distribution in Greece Supplement Table 1. Freshwater crayfish distribution in Greece based on comprehensive literature review, sampling and internet (citizen-sciense) data. ASA: Astacus astacus; APT: Austropotamobious torrentium; ASL: Astacus leptodactylus; PFL: Pacifastacus leniusculus. Tributary/ river basin/ Year Altitude Map # Species Location lake/ mountain Region Source (sample/ Specific comment Extra comment (a.s.l.) info/ source) 1 ASA Doirani lake Doirani lake Central 140 Balss, 1928; Karaman, 1963 1917 (Balss)- Sample (4 specimens-Balss), Karaman Historical records. Probably, Macedonia 1960s also worked on numerus specimens not current presence in the lake (Karaman) 2 ASA Tributary of Doirani Doirani lake Central 176 Perdikaris, 2009 mid 2000s Sample Koutrakis E., pers. comm. lake Macedonia 3 ASA Axios river and Axios river and tributaries Central 30 Balss, 1928; Holthuis, 1961; from 1918 Sample (1 specimen-Balss, 1928), Probably not current presence in the main tributaries Macedonia Karaman, 1963; Albrecht, (Balss) Holthuis (1961), Karaman (1963) and river body. Before 1963 (Kinzelbach, 1982; Kinzelbach, 1986 Kinzelbach (1986) also worked on 1986) in the lower (Greek) part specimens 4 ASA Axioupoli Tributary of Axios river Central 72 Perdikaris, 2009 2007 Michailidis G. (pers. comm.) based on Fisheries Dept of Kilkis Macedonia sample 5 ASA Artificial lake Paiko mountain (northern Central 275 Perdikaris, 2009 2007 Michailidis G. (pers. comm.) based on Fisheries Dept of Kilkis Metalleiou part) Macedonia sample 1 6 ASA Blue lake, Metalliou Paiko mountain Paiko Central 420 Current work 2013 http://www.pmnews.gr/travel/699- lake (and brooks), (northern part) Macedonia paiko-n-kilkis-taksideiontas-ypo-skian Kilkis 7 ASA Close to Koupa Paiko mountain (northern Central 560 Perdikaris, 2009 2007 Michailidis G. (pers. comm.) based on Fisheries Dept of Kilkis village part) Macedonia sample 8 ASA Close to Skra village Paiko mountain (northern Central 685 Perdikaris, 2009 2007 Michailidis G. (pers. comm.) based on Fisheries Dept of Kilkis part) Macedonia sample 9 ASA Spring close to Tributary of Loudias river, Central 41 Current work 2016 Kehagias P. (pers. comm.) Aravissos village Paiko mountain Paiko Macedonia (southern part) 10 ASA Melisiotikos river Tributary of Loudias river, Central 8 Koutrakis et al., 2007; 2006 Sample (3 specimens-Koutrakis et al., Paiko mountain (southern Macedonia Perdikaris, 2009 2007; Perdikaris, 2009) part) 11 ASA Palaifyto Tributary of Loudias river, Central 12 Laggis et al., 2017 2011-2012 Sample (20 specimens-Laggis et al., Translocated probably from Paiko mountain (southern Macedonia 2017) Vegoritida/Agra lake part) 12 ASA Agios Nikolaos (3 km springs of Arapitsa river Central 491 Koukoura, 1973 1965 Sample Mentioned as A. astacus balcanicus SW of Naousa) (Vermio mountain), tributary Macedonia of Aliakmonas river 13 ASA Vegoritida lake Vegoritida lake Central 510 Koukoura, 1973; Laggis et 1968, 2008- Sample (unknown number of specimens Mentioned as A. astacus balcanicus by Macedonia al., 2017 2009 in Koukoura, 1973, 1 specimen-Laggis Koukoura (1973). Translocated from et al., 2017) Orchomenos and Edessa (Laggis et al., 2 2017) 13 ASA Vegoritida lake Vegoritida lake Central 510 Current work 2010 https://www.youtube.com/watch?v=77B Crayfish in the lake but not adequately Macedonia 8TogTSUY visible for identification 14 ASA Skotina stream Tributary of Ziliana on Central 1 203 Laggis et al., 2017 2012 Sample (20 specimens) Native Olympos mountain Macedonia 15 ASA Karya village Tributary of Ziliana on Central 817 Laggis et al., 2017 2011-2012 Sample (20 specimens) Native Olympos mountain Macedonia 16 ASA Prespa lakes and Prespa lakes watershed Western 842 Krstić, 2012; Spirkovski et ? Krstić, S. S. (2012); Spirkovski et al., Krstić (2012) mentions ASA and APT for adjacent tributaries Macedonia al., 2012 (2012). Megali (Macro) Prespa watershed. Spirkovski et al., (2012) mentions ASA with the tributaries of lakes as the main habitats but the specimen shown in photo is APT. Moreover the catching of crayfish is at present forbidden due to low population density. Both species are rather present in the system 17-23 ASA Kipoi (23) and Tributary of Aoos river Epirus 1 086 Theocharis, 1986; Machino 1980s, 2011 Filos P. (pers. comm.) (Fisheries Dept of Present in dense network of mountain mountain tributaries 935 and Holdich, 2006; Kastoria). Sample (2 specimens from streams close to villages Tsepelovo (17), of Voidomatis river 670 Koutrakis et al., 2007; Fragades-Laggis et al., 2017) Fragades (18), Negrades (19), Dilofo (20), 872 Perdikaris, 2009; Laggis et Aspraggeloi (21) and Elati (22). Aslo 960 al., 2017 some dots on a map by Theocharis (1986) 973 3 775 24 ASA Upper segments of Kalamas river Epirus 380 Theocharis, 1986; from 1980s Sample (numerous specimens- Commercially exploited population (local Kalamas river and Kevrekidis et al., 1999; Perdikaris, 2009) fest in the village Vrontismeni) with tributaries Gormos Nikolaou, 2004; Perdikaris significant mortalities in the past due to and Limpousdas et al., 2005a; Machino and crayfish plague (in 1982-PFL, in 1992- (from springs close to Holdich, 2006; Perdikaris, stocking of juveniles from France and in Kalpaki down to the 2009; Laggis et al., 2017 2004 (Theocharis, 1986; Nikolaou, 2004; vilages Kataraktes and Savvidis, 2004). Translocated from Mazaraki) Larissa during 1980s’ and from uknown origins during 1990 and 1991. 25 ASA Tzaravina lake Epirus 466 Laggis et al., 2017 2011 Sample (6 specimens) Translocated from Larissa during 1980s’ and from uknown origins during 1990 and 1991. 26-30 ASA Grevenitis river (30) Tributaries of Arachthos Epirus 998 Perdikaris, 2009 2007 Filos P. (pers. comm.) (Fisheries Dept of Present in mountain brooks close to the river 866 Kastoria) villages Dikoryfo (26), Manassi (27), 828 Kalouta (28) and Dipotamo (29). 790 655 31 ASA Chani Kaber Aga Tributary of Arachthos river Epirus 574 Laggis et al., 2017 2011 Sample (6 specimens) 4 32 ASA Artificial lake of Aoos Artificial lake of Aoos Epirus 1 348 Nikolaou, 2004; Koutrakis 2004, 2009, Samples (15 specimens-Nikolaou, 2004 According to Nikolaou (2004) the lake springs (and 10 springs et al., 2007; Perdikaris, 2011 and 40 specimens, 20 each from two was stocked with ASA in 1996 by the peripheral streams) 2009; Laggis et al., 2017 sampling sites-Laggis et al., 2017) Municipal Company of lake Pamvotis 32 ASA Artificial lake of Aoos Artificial lake of Aoos Epirus 1 348 Current work 2014 http://www.tovima.gr/society/article/?ai springs springs d=622718 33 ASA Krania village Tributary of Pinios river Western 1 220 Laggis et al., 2017 2011 Sample (6 specimens) Native Macedonia (very close to the border with Epirus) 34 ASA Pamvotida lake Pamvotida lake Epirus 470 Historical records by from 1930s to Sample (15 specimens-Karaman, 1963) Abundant in 1974, sample 1977 Athanssopoulos, 1935; late 1970s (Kinzelbach, 1986). Suspected incident of Karaman, 1963; Kinzelbach, crayfish plague during early 1980s' 1986; Theocharis, 1986; Paschos and Kagalou, 1995; Kevrekidis et al., 1999 34 ASA Pamvotida lake Pamvotida lake Epirus 470 Perdikaris and Georgiadis, 2015 Sample (4 specimens) Machino and Holdich (2006) did not 2017 found the species during a 1996 survey. Recent re-occurrence. 35 ASA Mountain streams Tributaries of Arachthos Epirus 860 Perdikaris, 2009 2007 Gouva E. (pers. comm.) close to Pramanta river 5 village 36 ASA Upper segments of Tributaries of Acheron river Epirus 392 Theocharis, 1986; From 1980s Unspecified location but some dots on a Acheron river Perdikaris, 2009 map in Theocharis (1986) (Diavolorema) 37 ASA Upper-midle Tributaries of Louros river Epirus 310 Theocharis, 1986; From 1980s Not present in the main river body (absent segments of Louros Perdikaris, 2009 during a 2008 survey by Perdikaris). Some river (down to dots on a map by Theocharis (1986). Kerasonas) 38 ASA Potsona stream Tributary of Louros river Epirus 131 Perdikaris, 2009 2005 Recent re-stocking 39 ASA Spring of Mavri close Tributary of Louros river Epirus 21 Current work at least up to Konstantinidis E. (unpub. data.) Probably historical record to Romio village 1950s 40 ASA Arachthos river Arachthos river Epirus 135 Laggis et al., 2017 2011 Sample (1 specimen) 41,42 ASA Akri Elassonas Armampei dam (41) and the Thessaly 1 083 Current work 2012 http://akrielassonas.blogspot.gr/2012/09/ dam was stocked from the nearby nearby Tsairisio waterfall 928 blog-post_20.html population. Photos available (42) 43 ASA Kefalovryso village, Spring Thessaly 255 Current work; Laggis et al., 2012 https://www.psarema.gr/sizitiseis/glika- Elassona 2017 nera/235588-karavides-glykon- neron?limitstart=0. Sample (1 specimen- Laggis et al., 2017) 44 ASA Close to the villages Dam in a tributary of Pinios Thessaly 1032 Current work; Laggis et al., 2011-2012 https://www.youtube.com/watch?v=HF Local festival. Translocation in Logga Achelinada and river 2017 G32C9Nnb4. Sample (17 specimens- from Krania during the last 10-15 years. 6 Logga, Kalampaka Laggis et al., 2017) 44 ASA Close to Achelinada Tributary of Pinios river Thessaly 1 032 Current work 2015 http://www.trikalaola.gr/%CE%95%CE Photos available of cooked specimens village, Kalampaka %BA%CE%B4%CE%B7%CE%BB%C F%8E%CF%83%CE%B5%CE%B9%C F%82/%CE%B1%CF%80%CF%8C%C F%88%CE%B5-%CE%B7- %CE%B3%CE%B9%CE%BF%CF%81 %CF%84%CE%AE- %CE%BA%CE%B1%CF%81%CE%B 1%CE%B2%CE%AF%CE%B4%CE% B1%CF%82- %CF%83%CF%84%CE%B7%CE%BD - %CE%B1%CF%87%CE%B5%CE%B B%CE%B9%CE%BD%CE%AC/ 45 ASA Brook close to Tributariy of Pinios river Thessaly
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