DOCSLIB.ORG

Improvement of Power Generation Using Shewanella Putrefaciens

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Improvement of Power Generation Using Shewanella Putrefaciens Bioresource Technology 166 (2014) 451–457 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Improvement of power generation using Shewanella putrefaciens mediated bioanode in a single chambered microbial fuel cell: Effect of different anodic operating conditions ⇑ Soumya Pandit a, Santimoy Khilari b, Shantonu Roy a, Debabrata Pradhan b, Debabrata Das a, a Department of Biotechnology, Indian Institute of Technology, Kharagpur, Kharagpur 721302, India b Materials Science Centre, Indian Institute of Technology, Kharagpur, Kharagpur 721302, India highlights Taguchi design to find out the key anodic process parameter. Impedance study to evaluate the influence of riboflavin addition to anolyte. Cyclic voltammetry to find out the effect of nano hematite modified anode. Microscopic study of biofilm formation using different nano hematite loaded anode. article info abstract Article history: Three different approaches were employed to improve single chambered microbial fuel cell (sMFC) Received 8 March 2014 performance using Shewanella putrefaciens as biocatalyst. Taguchi design was used to identify the key Received in revised form 18 May 2014 process parameter (anolyte concentration, CaCl2 and initial anolyte pH) for maximization of volumetric Accepted 21 May 2014 power. Supplementation of CaCl was found most significant and maximum power density of 4.92 Available online 29 May 2014 2 W/m3 was achieved. In subsequent approaches, effect on power output by riboflavin supplementation to anolyte and anode surface modification using nano-hematite (Fe2O3) was observed. Volumetric power Keywords: density was increased by 44% with addition of 100 nM riboflavin to anolyte while with 0.8 mg/cm2 Single chambered MFC nano-Fe O impregnated anode power density and columbic efficiency increased by 40% and 33% Riboflavin 2 3 Shewanella putrefaciens respectively. Cyclic voltammetry revealed improvement in electrochemical activity of Shewanella with Taguchi optimization nano-Fe2O3 loading and electrochemical impedance depicted inverse relationship between charge Nano hematite transfer resistance and nano-Fe2O3 loading. This study suggests anodic improvement strategies for maximization of power output. Ó 2014 Elsevier Ltd. All rights reserved. 1. Introduction promising technology, MFC suffers from limitations such as low power density, high cost etc. MFCs will be a viable option for power Microbial fuel cell (MFC) uses electrochemically active bacteria generation if the current production of this device is improved (EAB) as biocatalyst to convert biodegradable waste into electricity (Kim et al., 2007). The electrochemical performance of the (Yang et al., 2012). EABs are gaining importance because of their bio-anode is one of the major factors that affect the bioelectricity electron donating ability to the electrodes in MFC. Microorganisms generation process therefore identification of key operational fac- such as members of the Geobacter family, Shewanella putrefaciens tors and their optimization is of utmost important for the maximi- and Shewanella oneidensis, Rhodoferax ferrireducens, Pseudomonas zation of the power output (Pham et al., 2009). Electrode aeruginosa, Clostridium butyrium and Aeromonas hydrophila have modification is another strategy to improve the performance of been reported to oxidize organic matter at anode to complete their an MFC (Wei et al., 2011). Thus the present study endeavored in metabolism process (Logan and Regan, 2006). In spite of being a this direction and was focused in improving power generation using Shewanella sp. as anodic biocatalyst in a single chambered MFC (sMFC). ⇑ Corresponding author. Tel.: +91 3222 283758/9434717424; fax: +91 3222 Many MFCs operation were reported with Shewanella sp. as a 255303. E-mail address: [email protected] (D. Das). model organism (Kim et al., 2002; Sharma and Kundu, 2010) which http://dx.doi.org/10.1016/j.biortech.2014.05.075 0960-8524/Ó 2014 Elsevier Ltd. All rights reserved. 452 S. Pandit et al. / Bioresource Technology 166 (2014) 451–457 is a facultative anaerobic bacteria that can use a wide range of elec- (60% v/v); 0.425 g KCl; 2.028 g anhydride Na2SO4; 0.19 g CaCl2Á2H2O; tron acceptors and are classified in the group of dissimilatory 0.105 g MgSO4Á7H2O and 1.004 g yeast extract (Difco) were taken. metal-reducing bacteria (DMRB) (Lovley et al., 2004). Intact cells The anolyte pH was adjusted to 7 (unless stated otherwise) of anaerobically grown S. putrefaciens are electrochemically active (Khilari et al., 2014) with 1.0 M carbonate/bicarbonate buffer. and can grow in a MFC using anode as its terminal electron Desirable chemical oxygen demand (COD) was maintained by acceptor. The anode performance in Shewanella inoculated MFC is altering the Na-lactate concentration in the medium. Since the influenced largely by factors such as anolyte pH; inoculum age; unmodified lactate medium contained 0.19 g CaCl2, all concentra- concentration of electron donor: Calcium chloride (CaCl2) ribofla- tions of CaCl2 added for Taguchi optimization process were vin, etc (Kim et al., 2002; Park and Zeikus, 2002). Previous studies normalized to the un-modified medium. were carried out to evaluate the individual effect of these operating parameters in the anode chamber that influenced the activity of 2.2. Synthesis of nano Fe2O3 Shewanella. However, the combined effects of these parameters have not yet been explored. A better understanding of interrela- Fe2O3 nano structure was prepared by a simple solvothermal tionship between the operational parameters is needed prior to method. In a typical synthesis, 30 mL 0.05 M ethanolic solution successful MFC application. The present study addressed the of FeCl3 was taken in a beaker and 360 mg urea was added to this individual as well as the combined effect of the above mentioned solution followed by stirring for 10 min. After stirring a clear solu- operating parameters. Taguchi method was adapted to evaluate tion was formed which was transferred to a 50 mL Teflon-line the influence of anolyte pH, CaCl2 and substrate concentration on stainless steel autoclave. Hydrothermal dwell temperature was volumetric power generation. set at 150 °C for 12 h. Then the autoclave was allowed to cool down Initially, it was assumed that outer most cytochrome C type naturally to room temperature and precipitate formed inside protein is involved in exocellular electron transfer (EET) and ribo- Teflon line container was collected by centrifugation. Subsequently flavin plays significant role in EET by donating electron to anode the product was washed with distilled water followed by ethanol (Kim et al., 2002). Recent work has shown that Shewanella species for several times. Finally the washed precipitate was dried in an secrete riboflavin that facilitates electron transfer to both insoluble oven at 60 °C for 6 h. metals and anodes. The removal of accumulated soluble flavins decreases the rate of electron transfer by Shewanella biofilm to 2.3. Physical characterizations of nano Fe2O3 electrodes over 80%. Commercial riboflavin is much cheaper con- sidering the requirement in anolyte of MFC (Marsili et al., 2008). The crystallographic phase and structure were evaluated from Thus, in our second approach, riboflavin was supplemented to ano- X-ray diffractogram. Powder X-ray diffraction was carried out in lyte to study the improvement in power output. Electrochemical a Rigaku Ultima III using Cu Ka (40 kV, 40 mA) radiation over a impedance spectroscopy was employed to estimate the reduction range of 20À80°. The surface morphology and composition was in internal resistance. studied with a Zeiss Supra scanning electron microscope at an In a third approach, the influence of anode modification accelerating voltage of 5 kV. (impregnated) with Nano Fe2O3 on power output was investigated. Anode activity of a MFC can be improved by introducing electroca- 2.4. Nano Fe2O3 decorated anode preparation pacitive component into it. It is well known that a capacitive anode can perform much efficiently than its noncapacitive counterpart. The anode was modified with nano Fe2O3 containing electrode So, incorporation of electrochemically charge storage materials modifier ink. In a typical ink preparation, requisite amount of nano 2 on anode surface is desirable. Now-a-days transitional metal Fe2O3 (0.2, 0.4, 0.8 mg/cm ) was dispersed in acetone-isopropyl oxides are extensively studied as electrode materials for electro- alcohol (1:1) solution with 5 wt% polytetrafluoroethylene (PTFE; chemical capacitors. Among these oxides Fe2O3 has gained much 60 wt%; Sigma Aldrich). The ink was subsequently sprayed on a attention for its low cost, non toxicity, high performance and long carbon cloth by a gravity spray gun. Then the modified carbon cycle life (Park et al., 2008). It was previously reported that Shewa- cloth was allowed to dry at 70 °C in an oven for 6 h. Finally the nella was attracted by hematite or Fe2O3 particles and it had a dried Fe2O3 loaded carbon cloth was used as anode in MFCs. tendency to reduce hematite (Bose, 2009). Thus hematite can be utilized as an anode modifier. In the present study, (hematite) 2.5. MFC assembly construction Fe2O3 nano structure was prepared, characterized and impreg- nated in the carbon cloth anode and its loading effect was evalu- Nine identical sMFCs were used for the experiments. The MFC ated on current output of sMFC. In addition, cyclic voltammetry consisted of an anode compartment and a membrane cathode was utilized to evaluate the capacitance of different nano Fe2O3 assembly (MCA) placed on opposite sides. The cuboidal anode loadings on anode of sMFC. chamber is made from transparent polyacrylic material with outer dimensions 7 Â 8 Â 3.5 cm3 of 110 mL capacity. The anode cham- ber had two ports at the top, one for electrode terminal and the 2. Methods other for reference electrode (Ag/AgCl, saturated KCl; +197 mV, Equiptronics, India) and sampling. The anode consisted of a carbon 2.1. Microbial strain, media and growth conditions cloth of working surface area 12 cm2 with a stainless steel wire welded to form the terminal. A low cost custom made KOH doped S.
Load more

Recommended publications
  • Antibacterial Activity and Mechanism of Linalool Against Shewanella Putrefaciens
    molecules Article Antibacterial Activity and Mechanism of Linalool against Shewanella putrefaciens Fengyu Guo 1,2,3, Qiong Liang 1, Ming Zhang 1, Wenxue Chen 1,2,3, Haiming Chen 1,2,3 , Yonghuan Yun 1,2,3 , Qiuping Zhong 1,2,3,* and Weijun Chen 1,2,3,* 1 College of Food Science and Technology, Hainan University, Haikou 570228, China; [email protected] (F.G.); [email protected] (Q.L.); [email protected] (M.Z.); [email protected] (W.C.); [email protected] (H.C.); [email protected] (Y.Y.) 2 Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Haikou 570228, China 3 Hainan Provincial Engineering Research Center of Aquatic Resources Efficient Utilization in the South China Sea, Haikou 570228, China * Correspondence: [email protected] (Q.Z.); [email protected] (W.C.) Abstract: The demand for reduced chemical preservative usage is currently growing, and natural preservatives are being developed to protect seafood. With its excellent antibacterial properties, linalool has been utilized widely in industries. However, its antibacterial mechanisms remain poorly studied. Here, untargeted metabolomics was applied to explore the mechanism of Shewanella putrefaciens cells treated with linalool. Results showed that linalool exhibited remarkable antibacterial activity against S. putrefaciens, with 1.5 µL/mL minimum inhibitory concentration (MIC). The growth of S. putrefaciens was suppressed completely at 1/2 MIC and 1 MIC levels. Linalool treatment reduced the membrane potential (MP); caused the leakage of alkaline phosphatase (AKP); and released the DNA, RNA, and proteins of S. putrefaciens, thus destroying the cell structure and expelling the cytoplasmic content.
    [Show full text]
  • Identification of the Specific Spoilage Organism in Farmed Sturgeon
    foods Article Identification of the Specific Spoilage Organism in Farmed Sturgeon (Acipenser baerii) Fillets and Its Associated Quality and Flavour Change during Ice Storage Zhichao Zhang 1,2,†, Ruiyun Wu 1,† , Meng Gui 3, Zhijie Jiang 4 and Pinglan Li 1,* 1 Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; [email protected] (Z.Z.); [email protected] (R.W.) 2 Jiangxi Institute of Food Inspection and Testing, Nanchang 330001, China 3 Beijing Fisheries Research Institute, Beijing 100083, China; [email protected] 4 NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Beijing Institute for Drug Control, Beijing 102206, China; [email protected] * Correspondence: [email protected]; Tel.: +86-10-6273-8678 † The authors contributed equally to this work. Abstract: Hybrid sturgeon, a popular commercial fish, plays important role in the aquaculture in China, while its spoilage during storage significantly limits the commercial value. In this study, the specific spoilage organisms (SSOs) from ice stored-sturgeon fillet were isolated and identified by analyzing their spoilage related on sensory change, microbial growth, and biochemical properties, including total volatile base nitrogen (TVBN), thiobarbituric acid reactive substances (TBARS), and proteolytic degradation. In addition, the effect of the SSOs on the change of volatile flavor compounds was evaluated by solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS). The results showed that the Pseudomonas fluorescens, Pseudomonas mandelii, Citation: Zhang, Z.; Wu, R.; Gui, M.; and Shewanella putrefaciens were the main SSOs in the ice stored-sturgeon fillet, and significantly affect Jiang, Z.; Li, P.
    [Show full text]
  • Bacterial Community Structure in a Sympagic Habitat Expanding with Global Warming: Brackish Ice Brine at 85€“90 °N
    The ISME Journal (2019) 13:316–333 https://doi.org/10.1038/s41396-018-0268-9 ARTICLE Bacterial community structure in a sympagic habitat expanding with global warming: brackish ice brine at 85–90 °N 1,11 1,2 3 4 5,8 Beatriz Fernández-Gómez ● Beatriz Díez ● Martin F. Polz ● José Ignacio Arroyo ● Fernando D. Alfaro ● 1 1 2,6 5 4,7 Germán Marchandon ● Cynthia Sanhueza ● Laura Farías ● Nicole Trefault ● Pablo A. Marquet ● 8,9 10 10 Marco A. Molina-Montenegro ● Peter Sylvander ● Pauline Snoeijs-Leijonmalm Received: 12 February 2018 / Revised: 11 June 2018 / Accepted: 24 July 2018 / Published online: 18 September 2018 © International Society for Microbial Ecology 2018 Abstract Larger volumes of sea ice have been thawing in the Central Arctic Ocean (CAO) during the last decades than during the past 800,000 years. Brackish brine (fed by meltwater inside the ice) is an expanding sympagic habitat in summer all over the CAO. We report for the first time the structure of bacterial communities in this brine. They are composed of psychrophilic extremophiles, many of them related to phylotypes known from Arctic and Antarctic regions. Community structure displayed strong habitat segregation between brackish ice brine (IB; salinity 2.4–9.6) and immediate sub-ice seawater (SW; – 1234567890();,: 1234567890();,: salinity 33.3 34.9), expressed at all taxonomic levels (class to genus), by dominant phylotypes as well as by the rare biosphere, and with specialists dominating IB and generalists SW. The dominant phylotypes in IB were related to Candidatus Aquiluna and Flavobacterium, those in SW to Balneatrix and ZD0405, and those shared between the habitats to Halomonas, Polaribacter and Shewanella.
    [Show full text]
  • Shewanella Putrefaciens – a New Opportunistic Pathogen of Freshwater Fish
    J Vet Res 60, 429-434, 2016 DE DE GRUYTER OPEN DOI: 10.1515/jvetres-2016-0064 G REVIEW ARTICLE Shewanella putrefaciens – a new opportunistic pathogen of freshwater fish Ewa Paździor Department of Fish Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland [email protected] Received: May 17, 2016 Accepted: August 31, 2016 Abstract In recent years, Shewanella putrefaciens, commonly known as a halophilic bacteria, has been associated with serious health disorders in freshwater fish. Therefore, it has been described as a new aetiological agent of the disease, named shewanellosis. S. putrefaciens is a heterogeneous group of microorganisms, belonging to the Alteromonadaceae family. Based on different criteria, three biovars and biogroups as well as four genomic groups have been distinguished. The first infections of S. putrefaciens in fish were reported in rabbitfish (Siganus rivulatus) and European sea bass (Dicentrarchus labrax L.). Outbreaks in farmed fish were reported in Poland for the first time in 2004. The disease causes skin disorders and haemorrhages in internal organs. It should be noted that S. putrefaciens could also be associated with different infections in humans, such as skin and tissue infections, bacteraemia, otitis. Investigations on pathogenic mechanisms of S. putrefaciens infections are very limited. Enzymatic activity, cytotoxin secretion, adhesion ability, lipopolysaccharide (LPS), and the presence of siderophores are potential virulence factors of S. putrefaciens. Antimicrobial resistance of S. putrefaciens is different and depends on the isolates. In general, these bacteria are sensitive to antimicrobial drugs commonly used in aquaculture. Keywords: freshwater fish, Shewanella putrefaciens, pathogenicity, virulence factors. Introduction that S. putrefaciens, considered as halophytic bacteria, has an ability to adapt to freshwater environment.
    [Show full text]
  • Draft Genomic Sequence of a Chromate- and Sulfate-Reducing
    Xia et al. Standards in Genomic Sciences (2016) 11:48 DOI 10.1186/s40793-016-0169-3 SHORT GENOME REPORT Open Access Draft genomic sequence of a chromate- and sulfate-reducing Alishewanella strain with the ability to bioremediate Cr and Cd contamination Xian Xia1, Jiahong Li1, Shuijiao Liao1,2, Gaoting Zhou1,2, Hui Wang1, Liqiong Li1, Biao Xu1 and Gejiao Wang1* Abstract Alishewanella sp. WH16-1 (= CCTCC M201507) is a facultative anaerobic, motile, Gram-negative, rod-shaped bacterium isolated from soil of a copper and iron mine. This strain efficiently reduces chromate (Cr6+) to the much 3+ 2− 2− 2− 2+ less toxic Cr . In addition, it reduces sulfate (SO4 )toS . The S could react with Cd to generate precipitated CdS. Thus, strain WH16-1 shows a great potential to bioremediate Cr and Cd contaimination. Here we describe the features of this organism, together with the draft genome and comparative genomic results among strain WH16-1 and other Alishewanella strains. The genome comprises 3,488,867 bp, 50.4 % G + C content, 3,132 protein-coding genes and 80 RNA genes. Both putative chromate- and sulfate-reducing genes are identified. Keywords: Alishewanella, Chromate-reducing bacterium, Sulfate-reducing bacterium, Cadmium, Chromium Abbreviation: PGAP, Prokaryotic Genome Annotation Pipeline Introduction Alishewanella sp. WH16-1 was isolated from mining The genus Alishewanella was established by Vogel et al., soil in 2009. This strain could resist to multiple heavy in 2000 with Alishewanella fetalis as the type species. It metals. During cultivation, it could efficiently reduce the belongs to the family Alteromonadaceae of the class toxic chromate (Cr6+) to the much less toxic and less 3+ 2− Gammaproteobacteria [1].
    [Show full text]
  • Shewanella Oneidensis
    RESEARCH ARTICLE Genome sequence of the dissimilatory metal ion–reducing bacterium Shewanella oneidensis John F.Heidelberg1,2, Ian T. Paulsen1,3, Karen E. Nelson1, Eric J. Gaidos4,5, William C. Nelson1, Timothy D. Read1, Jonathan A. Eisen1,3, Rekha Seshadri1, Naomi Ward1,2, Barbara Methe1, Rebecca A. Clayton1, Terry Meyer6, Alexandre Tsapin4, James Scott7, Maureen Beanan1, Lauren Brinkac1, Sean Daugherty1, Robert T. DeBoy1, Robert J. Dodson1, A. Scott Durkin1, Daniel H. Haft1, James F.Kolonay1, Ramana Madupu1, Jeremy D. Peterson1, Lowell A. Umayam1, Owen White1, Alex M. Wolf1, Jessica Vamathevan1, Janice Weidman1, Marjorie Impraim1, Kathy Lee1, Kristy Berry1, Chris Lee1, Jacob Mueller1, Hoda Khouri1, John Gill1, Terry R. Utterback1, Lisa A. McDonald1, Tamara V. Feldblyum1, Hamilton O. Smith1,8, J. Craig Venter1,9, Kenneth H. Nealson4,10, and Claire M. Fraser1,11* Published online 7 October 2002; doi:10.1038/nbt749 Shewanella oneidensis is an important model organism for bioremediation studies because of its diverse res- piratory capabilities, conferred in part by multicomponent, branched electron transport systems. Here we report the sequencing of the S. oneidensis genome, which consists of a 4,969,803–base pair circular chromo- http://www.nature.com/naturebiotechnology some with 4,758 predicted protein-encoding open reading frames (CDS) and a 161,613–base pair plasmid with 173 CDSs. We identified the first Shewanella lambda-like phage, providing a potential tool for further genome engineering. Genome analysis revealed 39 c-type cytochromes, including 32 previously unidentified in S. oneidensis, and a novel periplasmic [Fe] hydrogenase, which are integral members of the electron trans- port system.
    [Show full text]
  • To Multi-Cell Level Charge Transport in Geobacter Sulfurreducens DL-1
    ARTICLE Received 10 May 2013 | Accepted 10 Oct 2013 | Published 8 Nov 2013 DOI: 10.1038/ncomms3751 Probing single- to multi-cell level charge transport in Geobacter sulfurreducens DL-1 Xiaocheng Jiang1,*, Jinsong Hu2,*, Emily R. Petersen3, Lisa A. Fitzgerald4, Charles S. Jackan1, Alexander M. Lieber1, Bradley R. Ringeisen4, Charles M. Lieber1,5 & Justin C. Biffinger4 Microbial fuel cells, in which living microorganisms convert chemical energy into electricity, represent a potentially sustainable energy technology for the future. Here we report the single-bacterium level current measurements of Geobacter sulfurreducens DL-1 to elucidate the fundamental limits and factors determining maximum power output from a microbial fuel cell. Quantized stepwise current outputs of 92(±33) and 196(±20) fA are generated from microelectrode arrays confined in isolated wells. Simultaneous cell imaging/tracking and current recording reveals that the current steps are directly correlated with the contact of one or two cells with the electrodes. This work establishes the amount of current generated by an individual Geobacter cell in the absence of a biofilm and highlights the potential upper limit of microbial fuel cell performance for Geobacter in thin biofilms. 1 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA. 2 CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. 3 Nova Research, Inc., 1900 Elkin Street, Suite 230, Alexandria, Virginia 22308, USA. 4 Chemistry Division, US Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, District of Columbia 20375, USA. 5 School of Engineering and Applied Science, Harvard University, Cambridge, Massachusetts 02138, USA.
    [Show full text]
  • High Current Production of Shewanella Oneidensis with Electrospun Carbon Nanofiber Anodes Is Directly Linked to Biofilm Formation
    bioRxiv preprint doi: https://doi.org/10.1101/2021.01.28.428465; this version posted February 10, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. High current production of Shewanella oneidensis with electrospun carbon nanofiber anodes is directly linked to biofilm formation Johannes Erben∗ Xinyu Wang† Sven Kerzenmacher∗‡ February 5, 2021 1 Abstract We study the current production of Shewanella oneidensis MR-1 with electro- spun carbon fiber anode materials and analyze the effect of the anode morphol- ogy on the micro-, meso- and macroscale. The materials feature fiber diameters in the range between 108 nm and 623 nm, resulting in distinct macropore sizes and surface roughness factors. A maximum current density of (255 ± 71) µA cm−2 was obtained with 286 nm fiber diameter material. Additionally, micro- and mesoporosity were introduced by CO2 and steam activation which did not im- prove the current production significantly. The current production is directly linked to the biofilm dry mass under anaerobic and micro-aerobic conditions. Our findings suggest that either the surface area or the pore size related to the fiber diameter determines the attractiveness of the anodes as habitat and there- fore biofilm formation and current production. Reanalysis of previous works supports these findings. 2 Introduction 2.1 Shewanella oneidensis MR-1 and its applications in Bioelectrochemical Systems Duet to sumitted recent attempts to find alternatives to for ChemElectroChem the fossil-based economy, Bio- electrochemical systems (BES) moved into the focus of scientific interest.
    [Show full text]
  • A Novel Benthic Phage Infecting Shewanella with Strong Replication Ability
    viruses Article A Novel Benthic Phage Infecting Shewanella with Strong Replication Ability Zengmeng Wang 1,2, Jiulong Zhao 1,2, Long Wang 1, Chengcheng Li 1,2, Jianhui Liu 3, Lihua Zhang 3 and Yongyu Zhang 1,2,* 1 Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; [email protected] (Z.W.); [email protected] (J.Z.); [email protected] (L.W.); [email protected] (C.L.) 2 University of Chinese Academy of Sciences, Beijing 100049, China 3 CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; [email protected] (J.L.); [email protected] (L.Z.) * Correspondence: [email protected]; Tel.: +86-532-80662680 Received: 4 November 2019; Accepted: 17 November 2019; Published: 19 November 2019 Abstract: The coastal sediments were considered to contain diverse phages playing important roles in driving biogeochemical cycles based on genetic analysis. However, till now, benthic phages in coastal sediments were very rarely isolated, which largely limits our understanding of their biological characteristics. Here, we describe a novel lytic phage (named Shewanella phage S0112) isolated from the coastal sediments of the Yellow Sea infecting a sediment bacterium of the genus Shewanella. The phage has a very high replication capability, with the burst size of ca. 1170 phage particles per infected cell, which is 5–10 times higher than that of most phages isolated before.
    [Show full text]
  • TPP Riboswitch Characterization in Alishewanella Tabrizica And
    Erschienen in: Microbiological Research ; 195 (2017). - S. 71-80 https://dx.doi.org/10.1016/j.micres.2016.11.003 TPP riboswitch characterization in Alishewanella tabrizica and Alishewanella aestuarii and comparison with other TPP riboswitches a,b,c d a e,f Elnaz Mehdizadeh Aghdam , Malte Sinn , Vahideh Tarhriz , Abolfazl Barzegar , d,∗∗ a,b,∗ Jörg S. Hartig , Mohammad Saeid Hejazi a Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran b Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran c Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran d Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany e Research Institute for Fundamental Sciences (RIFS), University of Tabriz, Tabriz, Iran f The School of Advanced Biomedical Sciences (SABS), Tabriz University of Medical Sciences, Tabriz, Iran a b s t r a c t Riboswitches are located in non-coding areas of mRNAs and act as sensors of cellular small molecules, regulating gene expression in response to ligand binding. The TPP riboswitch is the most widespread riboswitch occurring in all three domains of life. However, it has been rarely characterized in environ- mental bacteria other than Escherichia coli and Bacillus subtilis. In this study, TPP riboswitches located in the 5 UTR of thiC operon from Alishewanella tabrizica and Alishewanella aestuarii were identified and characterized. Moreover, affinity analysis of TPP binding to the TPP aptamer domains originated from A. tabrizica, A. aestuarii, E.coli, and B. subtilis were studied and compared using In-line probing and Sur- face Plasmon Resonance (SPR).
    [Show full text]
  • Dissimilatory Iron-Reducing Microorganisms Are Present And
    minerals Article Dissimilatory Iron-Reducing Microorganisms Are Present and Active in the Sediments of the Doce River and Tributaries Impacted by Iron Mine Tailings from the Collapsed Fundão Dam (Mariana, MG, Brazil) Carolina N. Keim 1,* , Jilder D. P. Serna 2, Daniel Acosta-Avalos 2, Reiner Neumann 3 , Alex S. Silva 1,4 , Diogo A. Jurelevicius 1, Raphael S. Pereira 1 , Pamella M. de Souza 1, Lucy Seldin 1 and Marcos Farina 5 1 Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro—UFRJ, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro 21941-902, RJ, Brazil; [email protected] (A.S.S.); [email protected] (D.A.J.); [email protected] (R.S.P.); [email protected] (P.M.d.S.); [email protected] (L.S.) 2 Centro Brasileiro de Pesquisas Físicas—CBPF, Av. Xavier Sigaud 150, Urca, Rio de Janeiro 22290-180, RJ, Brazil; [email protected] (J.D.P.S.); [email protected] (D.A.-A.) 3 Centro de Tecnologia Mineral—CETEM, Av. Pedro Calmon 900, Cidade Universitária, Rio de Janeiro 21941-908, RJ, Brazil; [email protected] 4 Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo–UNIFESP, São Paulo 04023-062, SP, Brazil 5 Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro–UFRJ, Av. Carlos Chagas Filho, 373, Citation: Keim, C.N.; Serna, J.D.P.; Cidade Universitária, Rio de Janeiro 21941-902, RJ, Brazil; [email protected] Acosta-Avalos, D.; Neumann, R.; * Correspondence: [email protected] Silva, A.S.; Jurelevicius, D.A.; Pereira, R.S.; de Souza, P.M.; Seldin, L.; Farina, Abstract: On 5 November 2015, a large tailing deposit failed in Brazil, releasing an estimated 32.6 to M.
    [Show full text]
  • First Isolation and Whole-Genome Sequencing of a Shewanella Algae
    Alves et al. BMC Microbiology (2020) 20:360 https://doi.org/10.1186/s12866-020-02040-x RESEARCH ARTICLE Open Access First isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil Vinicius Buiatte de Andrade Alves1* , Eneas Carvalho2, Paloma Alonso Madureira1, Elizangela Domenis Marino1, Andreia Cristina Nakashima Vaz1, Ana Maria Centola Vidal1 and Vera Letticie de Azevedo Ruiz1 Abstract Background: Infections caused by Shewanella spp. have been increasingly reported worldwide. The advances in genomic sciences have enabled better understanding about the taxonomy and epidemiology of this agent. However, the scarcity of DNA sequencing data is still an obstacle for understanding the genus and its association with infections in humans and animals. Results: In this study, we report the first isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil using the boot sock method, as well as the resistance profile of this strain to antimicrobials. The isolate was first identified as Shewanella putrefaciens, but after whole-genome sequencing it showed greater similarity with Shewanella algae. The strain showed resistance to 46.7% of the antimicrobials tested, and 26 resistance genes were identified in the genome. Conclusions: This report supports research made with Shewanella spp. and gives a step forward for understanding its taxonomy and epidemiology. It also highlights the risk of emerging pathogens with high resistance to antimicrobial formulas that are important to public health. Keywords: Genome, Shewanella, Taxonomy, Swine, Resistance Background molecular biology, Shewanella was assigned as a new Shewanella spp. are Gram-negative bacteria mostly genus in the family Vibrionaceae [10].
    [Show full text]