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World Society for Virology | 2021| Virtual | Abstracts Platinum Sponsor Gold Sponsors Silver Sponsor The World Society for Virology (WSV) is a non-profit organization established in 20171 to connect virologists around the world with no restrictions or boundaries, and without membership fees. The WSV brings together virologists regardless of financial resources, ethnicity, nationality or geographical location to build a network of experts across low-, middle- and high-income countries.2 To facilitate global interactions, the WSV makes extensive use of digital communication platforms.3-8 The WSV’s aims include fostering scientific collaboration, offering free educational resources, advancing scientists’ recognition and careers, and providing expert virology guidance. By fostering cross-sectional collaboration between experts who study viruses of humans, animals, plants and other organisms as well as leaders in the public health and private sectors, the WSV strongly supports the One Health approach. The WSV is a steadily growing society with currently more than 1,480 members from 86 countries across all continents. Members include virologists at all career stages including leaders in their field as well as early career researchers and postgraduate students interested in virology. The WSV has established partnerships with The International Vaccine Institute, the Elsevier journal Virology (the official journal of the WSV) and an increasing number of other organizations including national virology societies in China, Colombia, Finland, India, Mexico, Morocco and Sweden. Abdel-Moneim AS, Varma A, Pujol FH, Lewis GK, Paweska JT, Romalde JL, Söderlund-Venermo M, Moore MD, Nevels MM, Vakharia VN, Joshi V, Malik YS, Shi Z, Memish ZA (2017) Launching a Global Network of Virologists: The World Society for Virology (WSV) Intervirology 60: 276–277. 2. Abdel-Moneim AS, Moore MD, Naguib MM, Romalde JL, Söderlund-Venermo M. WSV 2019: The First Committee Meeting of the World Society for Virology (2020) Virologica Sinica 35: 248-252. Steering Committee Ahmed Abdel-Moneim Founding President Maria Söderlund-Venermo (Head of the SOC) Anupam Varma WSV-President Mariana Baz Flor Pujol WSV-Vice President, Latin America Marietjie Venter Maria Söderlund-Venermo WSV-Vice President, Europe Michael Nevels Marietjie Venter WSV-Vice President, Africa Modra Murovska Michael Nevels WSV-General Secretary Richard Kuhn Richard Kuhn WSV President-Elect Rosa M. del Angel Sayeh Ezzikouri Scientific Organizing Committee Vikram Vakharia Ahmed Abdel-Moneim Vinod Joshi Anupam Varma William Wilson Cristiana Nascimento-Carvalho Yashpal Malik Douglas Gladue Yigang Tong Elsayed Abdelwhab Zhengli Shi Emma Poole Eric Delwart Finance Committee Flor Pujol Marietjie Venter Hanu Pappu Matthew Moore Janusz Tadeusz Pawęska Michael Nevels Jerome Kim Zhengli Shi Jesus Romalde Laura Kramer 1 World Society for Virology | 2021| Virtual | Abstracts Keynote Speakers PLENARY I | SARS-COV-2: EVOLUTION PARALLEL 2 | PLANT VIRUSES AND CONTROL Neena Mitter 13 Zhengli Shi 4 Kristiina Mäkinen 14 Marion Koopmans 4 Hanu Pappu 14 Neil Ferguson 5 James Van Etten 15 Jerome Kim 5 PARALLEL 3 | NEW WINDS IN VIRUS PLENARY II | PATHOGENESIS AND DIAGNOSTICS IMMUNE RESPONSES Klaus Hedman 15 Stanley Perlman 5 Evgeny Nikolaev 16 Bart Haagmans 5 Christina Wege 16 Kari Nadeau 6 Cameron Myhrvold 17 Emilia Liana Falcone 6 PARALLEL 4 | ANIMAL VIRUSES PLENARY III | FROM SMALL TO GIANT Anne Balkema-Buschmann 17 THROUGH THE AGES Marietjie Venter 18 Teresa de los Santos 18 Vincent Racaniello 7 Covadonga Alonso 19 Chantal Abergel 8 Edward Holmes 8 PARALLEL 5 | CLINICAL VIROLOGY Murilo Zerbini 9 Heikki Hyöty 19 Fabien Zoulim 20 PLENARY IV | ONE HEALTH, ONE WORLD Jean Rommelaere 20 Curtis Suttle 9 Stephanie Karst 21 Andrea Marzi 10 Linda Saif 10 PARALLEL 6 | PHAGE AND INSECT Robert Gallo 11 VIRUSES Karyn Johnson 21 PARALLEL 1 | AVIAN AND AQUATIC Myléne Ogliastro 22 VIRUSES Sylvain Moineau 22 Kanta Subbarao 11 Mzia Kutateladze 22 Khatijah Yusoff 12 Egbert Mundt 12 Øystein Evensen 13 Event Organiser www.vetlink.co.za 2 World Society for Virology | 2021| Virtual | Abstracts Submitted Talks SARS-CoV-2: Disease & Pathogenesis 24 SARS-CoV-2: Immunity 31 SARS-CoV-2: Diagnosis & Intervention 36 SARS-CoV-2: Molecular Virology 43 SARS-CoV-2: Transmission & Epidemiology 46 Human Viruses: Clinical Virology 49 HIV & Hepatitis Viruses 57 Human Viruses: Molecular Virology 63 Zoonotic Viruses: Clinical Virology 71 Zoonotic Viruses: Molecular Virology 76 Animal Viruses: Molecular Virology 83 Veterinary Virology 86 Animal Viruses: Epidemiology 90 Novel And Endogenous Animal Viruses 95 Plant Viruses: Molecular Virology 99 Novel Plant Viruses 106 3 World Society for Virology | 2021| Virtual | Abstracts _____________Keynote speakers_____________ SARS-CoV-2: Evolution and Control From SARS-CoV To SARS-CoV-2, Understanding Of Interspecies Infection Of Bat Coronaviruses Zhengli Shi Wuhan Institute of Virology, China SARS and COVID-19 are severe human respiratory diseases caused by two SARS-related coronaviruses (SARSr-CoV), SARS-CoV and SARS-CoV-2, respectively, in a 17 year interval. Although these two viruses share 79% genomic sequence identity, they use the same receptor, angiotensin converting enzyme (ACE2), for cell entry. The discovery of SARSr-CoVs in bats suggests that bats are natural reservoirs of SARS-CoV, probably SARS-CoV-2. Phylogenetic analysis based on RNA-dependent RNA polymerase divides the SARSr-CoVs into 2 lineages. SARS-CoV-1 lineage includes SARS-CoV from humans and civets and SARSr-CoVs from bats in China and Japan. SARS-CoV-2 lineage includes SARS-CoV-2 and SARSr-CoVs from pangolins captured by Chinese customs and from bats in China, Cambodia and Thailand. Protein sequence alignment based on the spike receptor binding domain (RBD) indicates that theses SARSr-CoVs can be divided into two clades, clade 1 has similar size as SARS-CoV, clade 2 has shorter size due to one or two peptide deletions. By RBD-ACE2 binding assay, pseudovirus or virus infection assays, it was demonstrated that the clade 1 can use human ACE2 at different efficiency, while clade 2 can’t. But clade 2 with one deletion uses bat ACE2 as a receptor, but with a low efficiency. These results suggest that some SARSr-CoVs have acquired the capability to deploy the human ACE2 as a receptor. Thus the future long-term surveillance is urgently needed to prevent the next emerging infectious disease caused by this group of viruses. Searching For The Origins Of SARS-CoV-2 Marion Koopmans Erasmus Medical Centre, Netherlands The first cases of COVID-19 were recognized in the city of Wuhan, where a clinician alerted the Wuhan CDC of an unusual case of pneumonia December 26th. Within a matter of days, it became clear that a larger cluster of cases occurred and that the cause was a new virus from the Sarbecovirus genus. Since then, the virus spread globally, causing a pandemic that will continue to challenge the world in the months and years to come. SARS COV 2 is genetically closest to RatG13, a virus that had been identified in faeces from bats in Yunnan province, but the genetic distance precludes a direct etiological link. In order to understand the origins of the pandemic, the WHO convened a joint 4 World Society for Virology | 2021| Virtual | Abstracts scientific mission that designed and conducted a series of studies to further our understanding of the first phase of the pandemic. The results of the first phase of these studies will be presented. How Vaccines And Variants Are Shaping Epidemiology And Policy In The COVID-19 Pandemic Neil Ferguson Vice-Dean (Academic Development), Faculty of Medicine, Imperial College London Abstract unavailable. COVID-19 Vaccines: Taking A Shot Beyond Efficacy Jerome Kim The International Vaccine Institute SNU Research Park, Republic of Korea Since the identification of COVID-19 and the SARS-CoV-2 pathogen, 15 months ago, 10 vaccines of various platforms have been shown to be safe and efficacious. Additional vaccines are anticipated in 2021 and 2022. Beyond the proof of efficacy and safety, a remarkable challenge lies in a series of additional challenges between the proof of efficacy and impact – successful mitigation of the burden of COVID-19 disease globally. To achieve 70% global coverage, we will need 10-14 billion doses, just for COVID-19, of high quality, safe and efficacious vaccines. We will need to vaccinate demographics not traditionally in the target population for vaccines in general use globally, which implies that the logistics and human capacity to ship, store, and administer COVID-19 vaccines will need to be strengthened globally. Finally there are a number of post efficacy scientific issues – dose and schedule optimization, correlates of protection, effectiveness studies, surveillance for variants, and pharmacovigiliance that may have profound implications for policy and the eventual control of SARS-CoV-2 worldwide. COVID-19: pathogenesis and immune responses Animal Models Of COVID-19 And Immune Responses Stanley Perlman University of Iowa, USA As the COVID-19 pandemic continues around the world, greater understanding of the immune response to vaccines and natural infections are required. Here, we describe the immune response in humans who survived the natural infection and were immunized with one or two doses of one of the LNP-mRNA vaccines. We measured virus-specific antibody and T cell responses before the first vaccination and after each of the two vaccinations. The first vaccine dose greatly increased
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  • Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: Exploring the Effects of High Hydrostatic Pressure on the Immune Recognition of Antigens

    Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: Exploring the Effects of High Hydrostatic Pressure on the Immune Recognition of Antigens

    bioRxiv preprint doi: https://doi.org/10.1101/330589; this version posted May 25, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens. Ancelmo Rabelo de Souzaa, Marriam Yamina, Danielle Gavac, Janice Reis Ciacci Zanellac, Maria Sílvia Viccari Gattia, Carlos Francisco Sampaio Bonafea, Daniel Ferreira de Lima Netoa,b* aDepartamento de Bioquímica e Biologia Tecidual e bDepartamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083- 862, Campinas, SP, Brazil. cEmbrapa Suínos e Aves, Laboratório de Virologia de Suínos, 89715-899, Concórdia, SC, Brazil. *Corresponding author: Tel.: +55 19 3521-6229; E-mail: [email protected] bioRxiv preprint doi: https://doi.org/10.1101/330589; this version posted May 25, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. ABSTRACT Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for developing new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at -18 °C, compared with a commercial vaccine produced using inactivated PPV.