Eur. J. Entomol. 104: 601–617, 2007 http://www.eje.cz/scripts/viewabstract.php?abstract=1264 ISSN 1210-5759 Phylogeny of European Dolichopus and Gymnopternus (Diptera: Dolichopodidae) and the significance of morphological characters inferred from molecular data MARCO VALERIO BERNASCONI1*, MARC POLLET2,3, MANUELA VARINI-OOIJEN1 and PAUL IRVINE WARD1 1Zoologisches Museum, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland; e-mail:
[email protected] 2Research Group Terrestrial Ecology, Department of Biology, Ghent University (UGent), K.L.Ledeganckstraat 35, B-9000 Ghent, Belgium 3Department of Entomology, Royal Belgian Institute of Natural Sciences (KBIN), Vautierstraat 29, B-1000 Brussels, Belgium Key words. Diptera, Dolichopodidae, Dolichopus, Ethiromyia, Gymnopternus, Europe, mitochondrial DNA, cytochrome oxidase I, cytochrome b, morphology, ecology, distribution Abstract. Dolichopodidae (over 6000 described species in more than 200 genera) is one of the most speciose families of Diptera. Males of many dolichopodid species, including Dolichopus, feature conspicuous ornaments (Male Secondary Sexual Characters) that are used during courtship. Next to these MSSCs, every identification key to Dolichopus primarily uses colour characters (postocular bristles; femora) of unknown phylogenetic relevance. The phylogeny of Dolichopodidae has rarely been investigated, especially at the species level, and molecular data were hardly ever involved. We inferred phylogenetic relationships among 45 species (57 sam- ples) of the subfamily Dolichopodinae on the basis of 32 morphological and 1415 nucleotide characters (810 for COI, 605 for Cyt-b). The monophyly of Dolichopus and Gymnopternus as well as the separate systematic position of Ethiromyia chalybea were supported in all analyses, confirming recent findings by other authors based purely on morphology.