animals Article Molecular Analysis of Antimicrobial Resistance among Enterobacteriaceae Isolated from Diarrhoeic Calves in Egypt Abdel-Moamen E. Meshref 1,† , Ibrahim E. Eldesoukey 1,*,† , Abdulaziz S. Alouffi 2,*, Saleh A. Alrashedi 3, Salama A. Osman 4 and Ashraf M. Ahmed 1 1 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt;
[email protected] (A.-M.E.M.);
[email protected] (A.M.A.) 2 King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia 3 Central Laboratory at Al Watania Poultry Company, Riyadh 51441, Saudi Arabia;
[email protected] 4 Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt;
[email protected] * Correspondence:
[email protected] (I.E.E.);
[email protected] (A.S.A.) † These authors contributed equally to this work. Simple Summary: Bacterial antimicrobial resistance is a serious global health challenge. This study investigated the occurrence of major antimicrobial resistance genes, including integrons, ß-lactamases, and florfenicol in Enterobacteriaceae that were isolated from diarrhoeic calves in Egypt. From 120 calves, 149 isolates of bacteria were recovered, identified, and screened phenotypically against 12 antimi- crobials, and molecularly for the presence of the resistance determinants of integrons, ß-lactamases and florfenicol. The findings revealed that 24.8% of the isolates exhibited multidrug resistance. Citation: Meshref, A.-M.E.; Escherichia coli was found to be the most prevalent multidrug resistant species. Class 1 integrons, Eldesoukey, I.E.; Alouffi, A.S.; blaTEM, and floR genes were detected at incidence rates of 18.8%, 24.8%, and 1.3%, respectively, Alrashedi, S.A.; Osman, S.A.; Ahmed, whereas class 2 integrons and blaCTX-M were not detected in any isolates.