DOCSLIB.ORG

Producing Transgenic Pigs for Xenotransplantation – Selection of Founder Animals and Establishment of Breeding Herds

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Producing Transgenic Pigs for Xenotransplantation – Selection of Founder Animals and Establishment of Breeding Herds Aus dem Department für Veterinärwissenschaften der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München Arbeit angefertigt unter der Leitung von Univ.-Prof. Dr. Eckhard Wolf (Re)producing transgenic pigs for xenotransplantation – selection of founder animals and establishment of breeding herds Inaugural Dissertation zur Erlangung der tiermedizinischen Doktorwürde der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München von Andrea Bähr aus München München 2011 Gedruckt mit der Genehmigung der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München Dekan: Univ.-Prof. Dr. Braun Berichterstatter: Univ.-Prof. Dr. Wolf Korreferent: Univ.-Prof. Dr. Zerbe Tag der Promotion 30. Juli 2011 Für den Puck TABLE OF CONTENTS 1 INTRODUCTION ......................................................................................1 2 REVIEW OF THE LITERATURE ..........................................................3 2.1 The pig as a model species in biomedical research ................................ 3 2.2 Established pig models .............................................................................. 4 2.2.1 Neurodegenerative diseases ........................................................................ 4 2.2.2 Cardiovascular diseases ............................................................................... 6 2.2.3 Diabetes mellitus ......................................................................................... 7 2.2.4 Cystic Fibrosis ............................................................................................. 9 2.3 Pigs as donor animals in the context of xenotransplantation .............. 11 2.3.1 Hyperacute xenograft rejection ................................................................. 12 2.3.2 Acute humoral xenograft rejection ............................................................ 14 2.3.3 Cell-mediated xenograft rejection ............................................................. 16 2.4 (Re)producing transgenic pigs ............................................................... 17 2.4.1 Pig breeds and pig breeding ...................................................................... 19 2.4.2 Inbreeding depression ............................................................................... 22 2.4.3 Inbreeding in pig livestock and experimental animals .............................. 24 2.5 Selection of breeding material – expression analysis ........................... 28 3 ANIMALS, MATERIALS AND METHODS ........................................33 3.1 Animals ..................................................................................................... 33 3.1.1 GalKO ....................................................................................................... 33 3.1.2 CD46 ......................................................................................................... 33 3.1.3 hTM ........................................................................................................... 34 3.1.4 HLA-E ....................................................................................................... 34 3.1.5 LEA29Y .................................................................................................... 35 3.2 Materials .................................................................................................. 36 3.2.1 Chemicals .................................................................................................. 36 3.2.2 Consumables ............................................................................................. 37 3.2.3 Devices ...................................................................................................... 38 3.2.4 Antibodies, drugs, enzymes, oligonucleotides, standards ......................... 39 I Table of contents 3.2.4.1 Antibodies ................................................................................................. 39 3.2.4.2 Drugs ......................................................................................................... 39 3.2.4.3 Enzymes .................................................................................................... 39 3.2.4.4 Oligonucleotides ........................................................................................ 40 3.2.4.5 Protein standards ....................................................................................... 42 3.2.5 Buffers, media, solutions ........................................................................... 42 3.2.6 Kits ............................................................................................................ 48 3.2.7 Others ........................................................................................................ 48 3.2.8 Software .................................................................................................... 48 3.3 Methods .................................................................................................... 49 3.3.1 Genomic analysis ...................................................................................... 49 3.3.1.1 Genotyping of founder animals and F1 generation ................................... 49 3.3.1.2 Duplex PCR ............................................................................................... 51 3.3.1.3 Agarose Gel Electrophoresis ..................................................................... 52 3.3.1.4 Southern Blot ............................................................................................. 52 3.3.1.5 Inverse Polymerase Chain Reaction (inverse PCR) .................................. 56 3.3.1.5.1 Genomic DNA isolation ............................................................................ 57 3.3.1.5.2 DNA fragmentation ................................................................................... 57 3.3.1.5.3 Circularisation of DNA fragments ............................................................ 58 3.3.1.5.4 Nested PCR ............................................................................................... 59 3.3.1.5.5 DNA Eluation ............................................................................................ 62 3.3.1.5.6 Ligation ..................................................................................................... 62 3.3.1.5.7 Heat Shock Transformation ...................................................................... 63 3.3.1.5.8 Plasmid preparation ................................................................................... 63 3.3.1.5.9 Restriction digest ....................................................................................... 64 3.3.1.5.10 PEG precipitation ...................................................................................... 64 3.3.1.5.11 Sequencing ................................................................................................ 65 3.3.1.5.12 EtOH precipitation .................................................................................... 66 3.3.2 Expression analysis ................................................................................... 66 3.3.2.1 Protein isolation ......................................................................................... 67 3.3.2.2 Enzyme-Linked Immunosorbent Assay (ELISA) ..................................... 67 II Table of contents 3.3.2.3 Immunohistochemistry .............................................................................. 69 3.3.3 Calculation of inbreeding coefficient ........................................................ 70 3.3.4 Design of breeding schedules .................................................................... 71 4 RESULTS ..................................................................................................73 4.1 Identification of suitable breeding herds .............................................. 73 4.2 Selection of founder animals .................................................................. 74 4.3 Expression and functional analysis of novel transgenic lines .............. 75 4.3.1 hTM ........................................................................................................... 75 4.3.1.1 Clarification of hTM transgene integration site ........................................ 79 4.3.2 INS-LEA ................................................................................................... 83 4.3.3 CAG-LEA ................................................................................................. 88 4.4 Breeding schedules .................................................................................. 92 4.5 Breeding & analysis of intermediate (F1) generations ........................ 99 4.6 F2 generations and hTM incorporation into breeding herd ............. 108 4.7 Identification of transgene zygosity ..................................................... 109 4.7.1 GalKO duplex PCR ................................................................................. 109 4.7.2 hTM duplex PCR .................................................................................... 111 5 DISCUSSION .........................................................................................113 6 SUMMARY .............................................................................................125 7 ZUSAMMENFASSUNG .......................................................................129 8 INDEX OF FIGURES ............................................................................133
Load more

Recommended publications
  • Xenotransplantation and the Harm Principle
    A peer-reviewed electronic journal published by the Institute for Ethics and Emerging Technologies ISSN 1541-0099 16(1) – June 2007 Xenotransplantation and the harm principle: Factoring out foreseen risk An Ravelingien, PhD Research Assistant, Department of Philosophy, Ghent University ([email protected] ) http://jetpress.org/v16/ravelingien.html Abstract Xenotransplantation – the transplantation, implantation, or infusion of live cells, tissues or organs from a nonhuman animal source into humans – has been suggested as the most imminent strategy to alleviate the shortage of human grafts. The pursuit of this technology is nonetheless restricted by an unquantifiable risk that the use of animal grafts will unleash new zoonoses that may affect the public at large. This paper is concerned with what the proper response to this public health threat should be. We will demonstrate that the regulatory measures taken to prevent secondary infections currently do not warrant full-blown protection of public health. That reality forces us to reconsider the extent to which the public should be guaranteed protection from a xenotransplant-related health hazard. In pondering that question, we will suggest that the permissibility of health hazards posed by emerging (bio)technologies is dependent on the perception that the benefits are both substantive and attainable and on the duty to account for foreseeable risks. In that sense, there is both good and bad news for the acceptability of xenotransplantation. An increased understanding of the infectious agents that are known to pose a health risk, relates the man-made health threat to risks that have a natural origin. 1 The risk of a xenogeneic pandemic On 18 January 2006, 90,628 patients were enlisted on waiting lists for an organ transplant across the US (OPTN 2006).
    [Show full text]
  • Actant Stories and the Australian Xenotransplantation Network
    Constructing and Fracturing Alliances: Actant Stories and the Australian Xenotransplantation Network Copyright - Neil Leslie, Wellcome Images; reproduced with permission Peta S. Cook BPhoto; BSocSc (Sociol.) (hons.) Humanities Research Program Queensland University of Technology Submitted in full requirement for the degree of Doctor of Philosophy 2008 “The XWP [Xenotransplantation Working Party] agree that, in retrospect, a sociologist would have been a useful addition to the group to help understand these issues” (Xenotransplantation Working Party 2004: 14, emphasis added). - i - Keywords sociology; xenotransplantation; transplantation; allotransplantation; actor-network theory; science and technology studies; public understanding of science (PUS); critical public understanding of science (critical PUS); scientific knowledge; public consultation; risk; animals - ii - Abstract Xenotransplantation (XTP; animal-to-human transplantation) is a controversial technology of contemporary scientific, medical, ethical and social debate in Australia and internationally. The complexities of XTP encompass immunology, immunosuppression, physiology, technology (genetic engineering and cloning), microbiology, and animal/human relations. As a result of these controversies, the National Health and Medical Research Council (NHMRC), Australia, formed the Xenotransplantation Working Party (XWP) in 2001. The XWP was designed to advise the NHMRC on XTP, if and how it should proceed in Australia, and to provide draft regulatory guidelines. During the period
    [Show full text]
  • Xenogeneic and Stem Cell-Based Therapy for Cardiovascular Diseases: Genetic Engineering of Porcine Cells and Their Applications in Heart Regeneration
    International Journal of Molecular Sciences Review Xenogeneic and Stem Cell-Based Therapy for Cardiovascular Diseases: Genetic Engineering of Porcine Cells and Their Applications in Heart Regeneration Anne-Marie Galow 1,* , Tom Goldammer 1,2 and Andreas Hoeflich 1 1 Institute of Genome Biology, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, Germany; [email protected] (T.G.); hoefl[email protected] (A.H.) 2 Molecular Biology and Fish Genetics Unit, Faculty of Agriculture and Environmental Sciences, University of Rostock, 18059 Rostock, Germany * Correspondence: [email protected]; Tel.: +49-38208-68-723 Received: 18 November 2020; Accepted: 15 December 2020; Published: 18 December 2020 Abstract: Cardiovascular diseases represent a major health concern worldwide with few therapy options for ischemic injuries due to the limited regeneration potential of affected cardiomyocytes. Innovative cell replacement approaches could facilitate efficient regenerative therapy. However, despite extensive attempts to expand primary human cells in vitro, present technological limitations and the lack of human donors have so far prevented their broad clinical use. Cell xenotransplantation might provide an ethically acceptable unlimited source for cell replacement therapies and bridge the gap between waiting recipients and available donors. Pigs are considered the most suitable candidates as a source for xenogeneic cells and tissues due to their anatomical and physiological similarities with humans. The potential of porcine cells in the field of stem cell-based therapy and regenerative medicine is under intensive investigation. This review outlines the current progress and highlights the most promising approaches in xenogeneic cell therapy with a focus on the cardiovascular system. Keywords: cell transplantation; myocardial infarction; mesenchymal stem cells; graft rejection; triple knockout pigs; genome editing; iPSCs; CRISPR/Cas 1.
    [Show full text]
  • Genetically Engineered Animals and Public Health
    GENETICALLY ENGINEERED ANIMALS AND PUBLIC HEALTH Compelling Benefits for Health Care, Nutrition, the Environment, and Animal Welfare Revised Edition: June 2011 GENETICALLY ENGINEERED ANIMALS AND PUBLIC HEALTH: Compelling Benefits for Health Care, Nutrition, the Environment, and Animal Welfare By Scott Gottlieb, MD and Matthew B. Wheeler, PhD American Enterprise Institute Institute for Genomic Biology, University of Illinois at Urbana-Champaign TABLE OF CONTENTS Abstract . 3 Executive Summary . 3 Introduction . 5 How the Science Enables Solutions . 6 Genetically Engineered Animals and the Improved Production of Existing Human Proteins, Drugs, Vaccines, and Tissues . 8 Blood Products . 10 Protein-Based Drugs . 12 Vaccine Components . 14 Replacement Tissues . 15 Genetic Engineering Applied to the Improved Production of Animals for Agriculture: Food, Environment and Animal Welfare . 19 Enhanced Nutrition and Public Health . 19 Reduced Environmental Impact. 21 Improved Animal Welfare . 21 Enhancing Milk . 22 Enhancing Growth Rates and Carcass Composition . 24 Enhanced Animal Welfare through Improved Disease Resistance . 26 Improving Reproductive Performance and Fecundity . 28 Improving Hair and Fiber . .30 Science-Based Regulation of Genetically Engineered Animals . 31 International Progress on Regulatory Guidance . 31 U. S. Progress on Regulatory Guidance . 31 Industry Stewardship Guidance on Genetically Engineered Animals . 32 Enabling Both Agricultural and Biomedical Applications of Genetic Engineering . 33 Future Challenges and Conclusion . 34 { GENETICALLY ENGINEERED ANIMALS AND PUBLIC HEALTH } Abstract Genetically engineered animals embody an innovative technology that is transforming public health through biomedical, environmental and food applications. They are inte- gral to the development of new diagnostic techniques and drugs for human disease while delivering clinical and economic benefits that cannot be achieved with any other approach.
    [Show full text]
  • Insideenglish1010-Fall2019.Pdf
    InsideEnglish1010 A Journal of First-Year Writing Volume 3 | Fall 2019 Inside English 1010 A Journal of First-Year Writing Lucy Bennett Chris Denis Kayla Griffith Olivia Lyle Anna Platt Natalie Smyth Ryan Tucker Conner Whitman Sophie Williams University of Wyoming | Department of English Inside English 1010 Volume 3 Fall 2019 Cover Photo Credit: Alyssa Canepa Inside English 1010 Department of English University of Wyoming 1000 East University Avenue Laramie, WY 82071 Staff Lead Editor: Mary Hill Production Editor: Heather Holland Editorial Intern: Kyle Moore Faculty Advisor: Nancy Small Founding Editor: Kelly Kinney Editorial Board Isiah Dale William Kingsland Kalie Leonard Lydia Stuver Acknowledgements I’m excited to see this third volume of Inside English 1010 come to fruition. It is a vivid illustration of the excellent, thought-provoking work going on in our first year writing program at the University of Wyoming. The heroes and heroines of this year’s collection are, of course, the undergraduate student writers. Their work demonstrates the varied interests and strong talents of UW students. In the following pages, you’ll find expository writing on ecotourism, on race and representation in popular movies, and on how Matthew Shepard’s murder impacted the UW university community. Digging into their own curiosities through exemplary researched academic arguments, this volume’s authors will persuade you to expand your views on xenotransplanation, on the regulation of palm oil, and on regulating media cov- erage of mass shottings. And in our final genre—op-eds aimed at broader public read- ers—students share informed opions on the benefits of co-ed sports, the physical nature of being in a marching band, and the potential harms of “powerbait” to fly fishing culture.
    [Show full text]
  • Xenotransplantation: Benefits and Risks Louisa Chapman Centers for Disease Control and Prevention, Atlanta, Georgia, USA
    Conference Panel Summaries able organic carbon level, degree of pipe corrosion, and portion of the pressure wave, a substantial amount of treatment/distribution system characteristics. Chloramine is contaminated water (>1 gal per minute) from the outside can considerably more effective than chlorine for controlling be pulled into pipes through a small leak. This problem is Legionella in biofilms, presumably because chloramine is aggravated when sewer lines are placed close to water pipes. more stable and thus less reactive than chlorine, allowing it to Dr. LeChevallier stated that a number of waterborne disease penetrate the biofilm more deeply. outbreaks have been linked to distribution system An important factor in distribution system contamina- deficiencies. Among the agents of nosocomial waterborne tion and bacterial growth on biofilms is transient water disease is MAC. This opportunistic bacterial pathogen lives in pressure fluctuations that create pressure waves that pass water, is resistant to water disinfection (much more so than through pipes in the distibution system. During the negative Giardia cysts), and grows in pipe biofilms. Xenotransplantation: Benefits and Risks Louisa Chapman Centers for Disease Control and Prevention, Atlanta, Georgia, USA During transitional periods new scientific understand- around two key concepts: pretransplant screening of some ings bring new questions. The pivotal issues in xenotrans- animal herds, source animals, and xenotransplantation plantation concern biohazards. The U.S. Public Health products to minimize the risk of xenogeneic infections with Service defines xenotransplantation as “. any procedure recognized pathogens and posttransplant surveillance of that involves the transplantation, implantation, or infusion recipients for previously unrecognized xenogeneic organisms. into a human recipient of either A) live cells, tissues, or organs Endogenous retroviruses exist as inactive proviral DNA from a nonhuman animal source or B) human body fluids, in the germline of all mammals adequately studied to date.
    [Show full text]
  • The Cultural, Spiritual and Ethical Aspects of Xenotransplantation [Bioethics Council]
    toi te taiao the BIOETHICS COUNCIL The Cultural, Spiritual and Ethical Aspects of Xenotransplantation: Animal-to-Human Transplantation bioethics.org.nz www A DISCUSSION DOCUMENT Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION Published in January 2005 by Toi te Taiao: the Bioethics Council PO Box 10362, Wellington, New Zealand ISBN: 0-478-18985-0 This document is available from the Bioethics Council website: www.bioethics.org.nz bioethics.org.nz www PAGE 1 Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION PAGE 2 Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION Contents Acknowledgements 5 Foreword 6 Join the Discussion 7 Introduction 10 Face-to-face dialogue 11 PART ONE > Xenotransplantation: Science, Safety and Effectiveness 14 1. What is Xenotransplantation? 14 The different types of xenotransplantation 14 The process of developing xenotransplantation 16 2. Why is Xenotransplantation Being Considered? 17 A victim of success 17 What are the possible alternatives? 18 3. How Well Does Xenotransplantation Work? 20 Animal external therapies 20 Animal cell therapies 20 Animal organ transplants 21 Which animals work best? 21 4. What are the Risks? 22 Cross-species infection 22 Informed consent 23 Public health risks 24 PART TWO > Xenotransplantation: Cultural, Spiritual and Ethical Issues 26 Introduction 26 5. Spirituality, Culture and Human Need 27 Religious viewpoints 27 Nature 27 Identity 28 Xenotransplantation and human need 29 6. Maori and Xenotransplantation 30 Contents continued PAGE 3 Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION Contents continued A range of Maori views 30 Questions 31 7. The Interests of Animals 32 Animals likely to be used in xenotransplantation 32 Genetic modification of animals 32 Animal welfare and ethics 33 Xenotransplantation involving genetic modification 33 8.
    [Show full text]
  • Una Voce 2012 I
    Una Voce 2012 i Una Voce 2012 Student Editors Gil Grissom, Reggie Jennings, Rhiannon Johns, Angel Jones, The Dang, Nicole Nave, Jamie Neisinger, Farrance Quins, Mekao Tramil Faculty Advisor Mary Fox Layout and Design by Dean Davis Photographs by Ann Williams ii Una Voce 2012 Introduction In 2001, two TCC writing teachers embarked on a project to publish the beautiful essays being written by Tacoma Community College students. They named the new magazine Una Voce, a Latin term meaning “with one voice.” But, in those 12 years, the voices we hear, and, especially, the channels through which we hear them, have changed dramatically. It’s no exaggeration to say that we are living in a different world. In the spring of 2001, the 9/11 terrorist attacks were still a couple of months away. Facebook? Wouldn’t happen for three more years. And now, here we are: Our country is at war. We have an African-American president. We don’t buy many books, but many of us read novels on our cell phones. Can you imagine what we’ll be doing 12 years from now? I can’t. Still, around the world and here on campus, people continue to write thoughtfully, often eloquently, about ideas and events about which they care deeply. In fact, it seems clear that we are writing more now than ever before, and we have a dizzying array of physical and digital tools at our disposal, constant access to these tools, and a never-ending parade of free on-line resources that encourage us to communicate with each other.
    [Show full text]
  • The Cultural, Ethical and Spiritual Aspects of Animal-To-Human Transplantation Bioethics.Org.Nz Www
    toi te taiao the BIOETHICS COUNCIL The Cultural, Ethical and Spiritual Aspects of Animal-to-Human Transplantation bioethics.org.nz www A report on xenotransplantation by Toi te Taiao: the Bioethics Council Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION .nz Published in August 2005 by Toi te Taiao: the Bioethics Council PO Box 10362, Wellington, New Zealand ISBN: 0-478-25931-X BC: 13 bioethics.org This document is available on the Bioethics Council website: www.bioethics.org.nz www PAGE 1 Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION PAGE 2 Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION Contents Letter from the Chair 4 1. Introduction 5 1.1 Background 5 1.2 Structure of this report 8 2. Developing the project 9 2.1 The dialogue process 9 2.2 Dialogue events 9 2.3 Online discussion forum 10 2.4 Written submissions 10 3. What the Bioethics Council heard 11 3.1 Culture, ethics, spirituality and human need 11 3.2 Mäori dialogue and deliberation 18 3.3 The interests of animals 20 3.4 Individual rights and public risk 24 4. The Bioethics Council’s thinking 27 4.1 Cultural, ethical and spiritual factors 27 4.2 Mäori and xenotransplantation 31 4.3 The interests of animals 32 4.4 Xenograft recipients and public health 33 5. Summary of recommendations 35 Appendix A: Reflections on the evaluation process 37 Appendix B: Glossary of terms 41 PAGE 3 Toi te Taiao: the Bioethics Council ANIMAL-TO-HUMAN TRANSPLANTATION Letter from the Chair It is with much pleasure that Toi te Taiao: the Bioethics Council presents the Minister for the Environment with this report into the cultural, ethical and spiritual aspects of xenotransplantation.
    [Show full text]
  • Eco–Terrorism Specifically Examining Stop Huntingdon Animal Cruelty (‘‘Shac’’)
    S. HRG. 109–1005 ECO–TERRORISM SPECIFICALLY EXAMINING STOP HUNTINGDON ANIMAL CRUELTY (‘‘SHAC’’) HEARING BEFORE THE COMMITTEE ON ENVIRONMENT AND PUBLIC WORKS UNITED STATES SENATE ONE HUNDRED NINTH CONGRESS FIRST SESSION OCTOBER 26, 2005 Printed for the use of the Committee on Environment and Public Works ( Available via the World Wide Web: http://www.access.gpo.gov/congress.senate U.S. GOVERNMENT PRINTING OFFICE 39–521 PDF WASHINGTON : 2008 For sale by the Superintendent of Documents, U.S. Government Printing Office Internet: bookstore.gpo.gov Phone: toll free (866) 512–1800; DC area (202) 512–1800 Fax: (202) 512–2104 Mail: Stop IDCC, Washington, DC 20402–0001 COMMITTEE ON ENVIRONMENT AND PUBLIC WORKS ONE HUNDRED NINTH CONGRESS FIRST SESSION JAMES M. INHOFE, Oklahoma, Chairman JOHN W. WARNER, Virginia JAMES M. JEFFORDS, Vermont CHRISTOPHER S. BOND, Missouri MAX BAUCUS, Montana GEORGE V. VOINOVICH, Ohio JOSEPH I. LIEBERMAN, Connecticut LINCOLN CHAFEE, Rhode Island BARBARA BOXER, California LISA MURKOWSKI, Alaska THOMAS R. CARPER, Delaware JOHN THUNE, South Dakota HILLARY RODHAM CLINTON, New York JIM DEMINT, South Carolina FRANK R. LAUTENBERG, New Jersey JOHNNY ISAKSON, Georgia BARACK OBAMA, Illinois DAVID VITTER, Louisiana ANDREW WHEELER, Majority Staff Director KEN CONNOLLY, Minority Staff Director (II) CONTENTS Page OCTOBER 26, 2005 OPENING STATEMENTS Inhofe, Hon. James M., U.S. Senator from the State of Oklahoma .................... 1 Jeffords, Hon. James M., U.S. Senator from the State of Vermont, prepared statement .............................................................................................................. 30 Lautenberg, Hon. Frank R., U.S. Senator from the State of New Jersey ........... 4 Thune, Hon. John, U.S. Senator from the State of South Dakota .....................
    [Show full text]
  • Transplantation Is Not Enough..., Or on the Concept of Xenotransplantation
    STUDIES IN LOGIC, GRAMMAR AND RHETORIC 52 (65) 2017 DOI: 10.1515/slgr-2017-0045 Ewa M. Guzik-Makaruk University of Bialystok, Faculty of Law Marta M. Perkowska University of Bialystok, Faculty of Pedagogy and Psychology TRANSPLANTATION IS NOT ENOUGH..., OR ON THE CONCEPT OF XENOTRANSPLANTATION Abstract. The paper presents the emergence of the concept of xenotransplan- tation which is a relatively new issue in the literature on the subject. It is due to the fact that transplants of animal organs are currently in the experi- mental phase. The main current problem of transplantology is the shortage of organs; hence, the search for new solutions has become an everyday challenge. If a way for the human body to tolerate animal organs could be found, transplant medicine and humanity would be in a completely different place. The authors introduce the concepts of transplantation and xenotransplantation and their origins, then they raise ethical issues related to this type of organ transplanta- tion. Finally, the authors conclude that xenotransplantations have a chance to be “incorporated” (back again) in the “transplants” category when the exper- imental phase of xenotransplantation shifts to the implementation stage. Time will tell whether it will be possible. Keywords: transplantation, xenotransplantation, heterotransplantation, medical experiment. Introduction The current key problem of transplantology is the deficiency of organs. By virtue of the fact that “nature abhors a vacuum” and it initiates on its own various actions to fill that void, people, drawing on familiar ways of coping in such cases, as for example filling the void in their own stomachs, undertake the idea of filling other “voids” in their own bodies, for example in the place of a sick kidney, with parts of animal bodies.
    [Show full text]
  • Position Paper of the Ethics Committee of the International Xenotransplantation Association
    Xenotransplantation 2003: 10: 194–203 Copyright Ó Blackwell Munksgaard 2003 Printed in UK. All rights reserved XENOTRANSPLANTATION ISSN 0908-665X THE ETHICS OF XENOTRANSPLANTATION Position Paper of the Ethics Committee of the International Xenotransplantation Association Sykes M, d’Apice A, Sandrin M. Position Paper of the Ethics Megan Sykes,1 Anthony d'Apice2 Committee of the International Xenotransplantation Association. and Mauro Sandrin3 (IXA Ethics Xenotransplantation 2003; 10: 194–2003. Ó Blackwell Munksgaard, 2003 Committee) 1Transplantation Biology Research Center, Abstract: Xenotransplantation (XTx) provides a potential solution to Massachusetts General Hospital/Harvard Medical the shortage of human organs and tissues, and has several advantages School, Boston, MA, USA, 2Department of Clinical over other possible solutions to this problem. However, a number of Immunology, St Vincent's Hospital, Fitzroy, Victoria, scientific and ethical barriers exist, and need to be addressed in order to Australia, and 3Austin Research Institute, Austin and advance the field of XTx in a manner that optimizes its potential to Repatriation Medical Center, Heidelberg, Victoria, benefit society and minimizes its risk. Some of the most pressing ethical Australia issues are discussed, and the position of the Ethics Committee of the International Xenotransplantation Association is presented. Key words: ethics – guidelines – infectious risk – pig – porcine – religion – retrovirus – XTx Address reprint requests to Megan Sykes, MD, Head, Bone Marrow Transplantation Section, Trans- plantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, MGH-East, Building 149-5102, 13th Street, Boston, MA 02129, USA (E-mail: megan.sykes@ tbrc.mgh.harvard.edu) Received 13 November 2002; Accepted 4 December 2002 and membership at large, and numerous thought- Introduction ful comments have been received in response.
    [Show full text]