bioRxiv preprint doi: https://doi.org/10.1101/761981; this version posted September 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Expressed barcodes enable clonal characterization of chemotherapeutic responses in chronic lymphocytic leukemia Aziz Al’Khafaji1,2*, Catherine Gutierrez3,4*, Eric Brenner1,2, Russell Durrett1,2, Kaitlyn E. Johnson2, Wandi Zhang4, Shuqiang Li4,5,6, Kenneth J. Livak4,6, Donna Neuberg7, Amy Brock1,2, Catherine J. Wu3,4,5,8 1 Institute of Cellular and Molecular Biology, University of Texas at Austin, TX, USA 2 Biomedical Engineering, University of Texas at Austin, TX, USA 3 Harvard Medical School, Boston, MA, USA 4 Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA 5 Broad Institute of MIT and Harvard, Cambridge, MA, USA 6 Translational Immunogenomics Lab, Dana-Farber Cancer Institute, Boston, MA, USA 7 Department of Data Sciences, Dana Farber Cancer Institute, Boston, MA, USA 8 Department of Medicine, Brigham and Women’s Hospital, Boston, MA, USA *Both authors contributed equally to this work. Author information: These authors jointly supervised this work: Catherine J. Wu and Amy Brock Correspondence should be addressed to
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[email protected] Key words: Chronic lymphocytic leukemia, CLL, clonal evolution, clonal dynamics, drug resistance, lineage tracing, DNA barcoding, expressed barcodes, single-cell RNA sequencing, cancer transcriptomics 1 bioRxiv preprint doi: https://doi.org/10.1101/761981; this version posted September 8, 2019.