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Karyomorphological Analysis of Erinocarpus, a Monotypic Endemic Genus from Western Ghats of India

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Karyomorphological Analysis of Erinocarpus, a Monotypic Endemic Genus from Western Ghats of India © 2012 The Japan Mendel Society Cytologia 77(1): 67–71 Karyomorphological Analysis of Erinocarpus, a Monotypic Endemic Genus from Western Ghats of India Anjali Dnyaneshwar Nalawade*, Kumar Vinod Chhotupuri Gosavi and Shrirang Ramchandra Yadav Department of Botany, Shivaji University, Kolhapur-416 004, (MS) India. Received July 14, 2011; accepted December 28, 2011 Summary Erinocarpus Nimmo ex J. Graham a monotypic endemic genus from Western Ghats represented by E. nimmonii J. Graham is distributed in central and northern Western Ghats. The present paper describes the distribution, meiotic count, mitotic count and karyotype analysis of the genus. The somatic chromosome number (2n)=48 and meiotic chromosome number (n)=24 are re- ported for the first time. The karyotype is of 2A Stebbins category of asymmetry and the karyotype formula is categorised as 2n=48=20m+28sm (2 SAT). Key word Erinocarpus nimmonii, India, Karyotype, Meiosis, Western Ghats. Erinocarpus Nimmo ex J. Graham is a monotypic endemic genus from Western Ghats (Mabberley 2005, Rana and Ranade 2009) categorised under the vulnerable category (VU) in the Red data book (Ahmedullah and Nayar 1990, Mishra and Singh 2001). It was treated under the tribe Grewieae in the family Tiliaceae by Bentham and Hooker (1862–1867) and Schumann (1895). Hutchinson (1967), however, treated Erinocarpus under the tribe Triumfettae based on fruit charac- ters. Presently it is under the sub-family Grewioideae in the family Malvaceae (Judd and Manchester 1997, Bayer et al. 1999). Erinocarpus nimmonii grows along the stream or riverbanks and hill slopes of moist decidu- ous forests at an altitude of 600–700 m in central and northern Western Ghats. Economically it is less well known. The bark fiber is used in rope making (Mabberley 2001) and the seed as famine food (Freedman 2005). The genus remained neglected from a cytological point of view and the present work aims at determining chromosome counts and karyotype analysis of E. nimmonii. Materials and ｍethods Seeds of E. nimmonii were collected from the Malkapur (N 16°53.215 and E 073°52.613) re- gion in the Kolhapur district. Seeds were germinated between 2 moist blotting papers in glass petri- plates. Mitosis was studied from the root tips of germinated seeds. Root tips of 6–10 mm length were pretreated with 0.1% colchicine solution for 3 to 4 h at 9∓3°C. The root tips were squashed in 2% propionic orcein. The well-spread somatic plates were photographed with a Coolpix-4500 Nikon camera at 1000⊗ magnification under a Jenaval microscope. Ten well-spread somatic chro- mosomes plates were analyzed for karyotype analysis using Levan et al. (1964) formulae. Floral buds were fixed in Cornoyʼs fluid and anthers were squashed in 2% propionic-orcein for meiotic study. Voucher specimens (SUK 5979) are deposited in the SUK Herbarium, Department of Botany, Shivaji University, Kolhapur. * Corresponding author, e-mail: [email protected] 68 A. D. Nalawade et al. Cytologia 77(1) Fig. 1. Erinocarpus nimmonii J. Graham.: a: Flower; b: Fruit; c: Somatic plate showing 2n=48; d: Meiocyte showing haploid chromosome number n=24; e: ideograph. Results Somatic chromosome number 2n=48 (Fig. 1c) and haploid chromosome number n=24 (Fig. 1d) is recorded in E. nimmonii. Chromosomes ranged from 2.76 to 1.44 μm in length. The chromo- somes of the species could be classified into 4 types on the basis of chromosome morphology (Tables 3 and 4). The first pair of chromosomes is very long with sub-median constriction and sat- ellites, followed by 3 pairs of long chromosomes with median to sub-median constrictions, fol- lowed by 16 pairs of medium chromosomes with median and sub-median constrictions, and the re- maining 4 pairs are small with median constrictions. The chromosomes of the 1st and 9th pairs were observed to possess satellites on the distal region of long arm. The karyotype analysis of E. nimmonii can be summarized as: diploid chromosome number (2n)=48, haploid chromosome number (n)=24, mean chromosome length: 2.06 μm, total chromo- some length of haploid compliment (TCLH): 49.47 μm, range of total chromosome length percent- age (TCL%): 5.58–2.91, gradient Index (GI): 54.35. symmetric Index (SI): 62.20 and total form percentage (TF%): 38.35. Karyotype formula 2n=48=20m+28sm (2SAT). Karyotype is of 2A cate- gory of asymmetry classification (Stebbins 1971). 2012 Karyomorphological Analysis of Erinocarpus, a Monotypic Endemic Genus from Western Ghats of India 69 Table 1. Grewia species and their chromosome counts Sr. no. Species 2n/n Author 1. Grewia abutifolia Ventenat ex Jussieu n=9 Sarkar et al. (1973) 2. G. asiatica L 2n=36 Singhal et al. (1982) 3. G. aspera Roxb. 2n=18, 18+2B Krishnappa and Munirajappa (1982) 4. G. disperma Rottb. 2n=18 Krishnappa and Munirajappa (1982) 5. G. elastica Royle I=9 Gill et al. (1979 and 1990) 6. G. hainesiana Hole I=18 Gill et al. (1979); Singhal et al. (1982) 7. G. hainesiana Hole 2n=27, 36 n=18 Gill et al. (1990) 8. G. hainesiana Hole 2n=27 Gill et al. (1979a) 9. G. hirsuta Vahl n=9 Singhal et al. (1980, 1982, 1984) 10. G. laevigata Vahl n=9 Bir et al. (1980); Singhal et al. (1982) 11. G. leptopetala Brandis n=18 Gill et al. (1979 and 1990), Singhal et al. (1982) 12. G. oppositifolia Buch.-Ham. n=9 Bir et al. (1980); Singhal et al. (1982) 13. G. optiva Drumm. n=9 Khatoon and Ali (1993) 14. G. vestita Wall. n=19 Sandhu and Mann (1988) 15. G. villosa Willd. 2n=36 Krishnappa and Munirajappa (1982) 16. G. welwitschii Burret 2n=18 Morawetz (1981b) Table 2. Triumfetta species and their chromosome counts Sr. no. Species 2n/n Author 1. Triumfetta annua L. 2n=20 Huang et al. (1989) 2. T. annua L. 2n=32 Krishnappa and Munirajappa (1980) 3. T. batramia L. 2n=32 Lay (1950) 4. T. macrophylla K. Schum. 2n=48 Jacob (1983) 5. T. pentandra L. 2n=16 Krishnappa and Munirajappa (1982) 6. T. pentandra L. n=16 Khatoon and Ali (1993) 7. T. pilosa Roth 2n=64 Krishnappa and Munirajappa (1980) 8. T. rhomboidea Jacq. 2n=32 Paiva and Leitao (1987) 9. T. rhomboidea Jacq. 2n=48 Krishnappa and Munirajappa (1980) 10. T. rotundifolia Lam. 2n=32 Krishnappa and Munirajappa (1982) 11. T. rotundifolia Lam. n=16 Sanjappa (1979) Discussion Erinocarpus nimmonii grows in moist to dry deciduous forests in central and Northern Western Ghats. It is categorized as vulnerable (Ahmedullah and Nayar 1990, Mishra and Singh 2001) as it has restricted distribution. Morphologically, the genus Erinocarpus Nimmo ex J. Graham is related to Grewia L., Heliocarpus L. and Triumfetta L. Morphological, anatomical and palynological studies support its close relationship with the neotropical Genus Heliocarpus (Van Heel 1966, Sharma 1969, Rajkumar 2001). The meiosis was found to be normal with bivalent counts n=24 (Fig. 1d). Mitosis was also found normal with 2n=48 (Fig. 1c). Centromeres were located in median to sub-median position of chromosomes. The first and ninth pairs of satellite chromosome were found in early metaphase while the secondary constrictions of these chromosomes were invisible in later stages probably due to heavy condensation (Okada 1975). With reference to the basic number x=8, Erinocarpus is closer to Triumfetta (Table 2) than to Grewia which has x=9 (Table 1). Chromosome numbers and karyotypic analysis of Triumfetta, Grewia and Heliocarpus need to be investigated to understand their interrelationship. 70 A. D. Nalawade et al. Cytologia 77(1) Table 3. Karyotype analysis of Erinocarpus nimmonii Chromo Long arm Short arm Total length d value r value i value Centromeric Type some pair L (μm) s (μm) c=l+s (μm) l/s l/s s/c⊗100 position 1 1.93∓1.03 0.83∓0.31 2.76∓1.22 1.1 2.33 30.07 sm A 2 1.46∓0.46 1.08∓0.55 2.54∓0.94 0.38 1.35 42.52 m B 3 1.63∓0.71 0.84∓0.41 2.51∓0.95 0.83 1.99 33.47 sm B 4 1.67∓0.52 0.85∓0.54 2.48∓0.88 0.78 1.92 34.47 sm B 5 1.5∓0.50 0.87∓1.59 2.37∓0.9 0.63 1.72 36.71 sm C 6 1.32∓0.62 0.97∓0.28 2.29∓0.81 0.35 1.36 42.36 m C 7 1.34∓0.66 0.86∓0.23 2.2∓0.84 0.48 1.56 39.09 sm C 8 1.34∓0.49 0.84∓0.44 2.18∓0.84 0.5 1.6 38.53 sm C 9 1.41∓0.47 0.75∓0.43 2.16∓0.84 0.66 1.88 34.72 sm C 10 1.22∓0.56 0.88∓0.35 2.1∓0.89 0.34 1.39 41.9 m C 11 1.22∓0.82 0.85∓0.23 2.07∓0.89 0.37 1.44 41.06 m C 12 1.22∓0.54 0.85∓0.42 2.07∓0.78 0.37 1.44 41.06 m C 13 1.24∓0.54 0.8∓0.42 2.04∓0.78 0.44 1.55 39.22 sm C 14 1.34∓0.49 0.68∓0.47 2.02∓0.78 0.66 1.97 33.66 sm C 15 1.21∓0.60 0.78∓0.36 1.99∓0.80 0.43 1.55 39.2 sm C 16 1.21∓0.60 0.78∓0.36 1.99∓0.80 0.43 1.55 39.2 sm C 17 1.3∓0.46 0.65∓0.40 1.95∓0.80 0.65 2 33.33 sm C 18 1.22∓0.42 0.7∓0.43 1.92∓0.80 0.52 1.74 36.46 sm C 19 1.19∓0.49 0.66∓0.39 1.85∓0.85 0.53 1.8 35.68 sm C 20 1.01∓0.60 0.8∓0.47 1.81∓0.76 0.21 1.26 44.2 m C 21 0.92∓0.38 0.75∓0.37 1.67∓0.74 0.17 1.23 44.91 m D 22 0.92∓0.40 0.64∓0.37 1.56∓0.74 0.28 1.44 41.02 m D 23 0.84∓0.42 0.66∓0.37 1.5∓0.71 0.18 1.27 44 m D 24 0.84∓0.32 0.6∓0.30 1.44∓0.60 0.24 1.4 41.67 m D Table 4.
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