Detectx® Progesterone Metabolites EIA Kit

NEW PRODUCT

PROGESTERONE METABOLITES EIA KIT

Use: Measure General Progesterone Metabolites Catalog Number: and Generate Reproductive Profiles K068-H1 (1 Plate Kit) Sample: Fecal Extracts and Urine K068-H5 (5 Plate Kit) Sensitivity: 51.2 pg/mL Time to Answer: 1.5 Hours Samples/Kit: 40 or 232 in Duplicate Stability: Liquid, 4°C Stable Reagents

Progesterone (P4, pregn-4-ene-3,20-dione) belongs to a class of hormones called progestogens and is an essential regulator of human femal reproductive function in the uterus, ovary, mamary gland, and brain. It also plays arole in the cardiovascular system. Progestogens are the primary hormones involved in the female menstural cycle, gestation, and embryogenesis of humans and most other species.

100 1.2

90 In different animal species, progesterone 1.0 is metabolized and excreted as a variety 80 of general progesterone by-products. %B/B0 70 Net OD molecules. Common metabolites include 0.8 5-reduced progesterones, pregnanolones, and 60 hydroprogesterones. Measurement of these 50 0.6 general progesterone metabolites provides vital data for studying reproductive and 40 0.4 survival strategies for endangered species. 30

20 0.2

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WWW.ARBORASSAYS.COM DetectX® Progesterone Metabolites EIA Kit

The DetectX® Progesterone Metabolite Immunoassay Kit, K068-H1/H5, was developed to quantitatively measure Progesterone metabolites present in extracted fecal samples and urine. This assay provides another valuable tool for profiling progestrone metabolites. The cross-reactivity profile of the newest assay includes important biomarkers absent from previous kits.

Arbor Assays Kit Comparison

Progesterone EIA Kit, Progesterone Metabolites EIA Kit, K025-H1/H5 K068-H1/H5 Standard Range 50-3,200 pg/mL 102.4-10,000 pg/mL Sensitivity 47.9 pg/mL 51.2 pg/mL Samples/Kit 39 or 231 in Duplicate 40 or 232 in Duplicate (1 plate & 5 plate) Validated Sample Fecal, Urine, TCM Fecal, Urine Type Time to Answer 150 Minutes 90 Minutes

Cross Reactivity Progesterone: 100% Progessterone: 100% Profile 3β-hydroxy-progesterone: 172% 5α-dihydroprogesterone: 56.7% 3α-hydroxy-progesterone: 188% 5β-dihydroprogesterone: 61.9% 11α-hydroxy-progesterone: 147% Epiallopregnanolone: 38.3% 11β-hydroxy-progesterone: 2.7% Pregnanolone: 41.2% 5α-dihydroprogesterone: 7.0% Allopregnandione: 27.3% Pregnenolone: 5.9% Pregnenolone: 17.6% Corticosterone: <0.1% Epipregnanolone: 10.2% Androstenedione: <0.1% Allopregnandiol: 0.29% 11α-hydroxyprogesterone: 4.9% 17α-hydroxyprogesterone: 5.7% 20α-hydroxyprogesterone: 0.34%