Detectx® Progesterone Metabolites EIA Kit

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Detectx® Progesterone Metabolites EIA Kit

NEW PRODUCT PROGESTERONE METABOLITES EIA KIT Use: Measure General Progesterone Metabolites Catalog Number: and Generate Reproductive Profiles K068-H1 (1 Plate Kit) Sample: Fecal Extracts and Urine K068-H5 (5 Plate Kit) Sensitivity: 51.2 pg/mL Time to Answer: 1.5 Hours Samples/Kit: 40 or 232 in Duplicate Stability: Liquid, 4°C Stable Reagents Progesterone (P4, pregn-4-ene-3,20-dione) belongs to a class of hormones called progestogens and is an essential regulator of human femal reproductive function in the uterus, ovary, mamary gland, and brain. It also plays arole in the cardiovascular system. Progestogens are the primary hormones involved in the female menstural cycle, gestation, and embryogenesis of humans and most other species. 100 1.2 90 In different animal species, progesterone 1.0 is metabolized and excreted as a variety 80 of general progesterone by-products. %B/B0 70 Net OD molecules. Common metabolites include 0.8 5-reduced progesterones, pregnanolones, and 60 hydroprogesterones. Measurement of these 50 0.6 general progesterone metabolites provides vital data for studying reproductive and 40 0.4 survival strategies for endangered species. 30 20 0.2 10 0 0.0 100 1000 10000 WWW.ARBORASSAYS.COM DetectX® Progesterone Metabolites EIA Kit The DetectX® Progesterone Metabolite Immunoassay Kit, K068-H1/H5, was developed to quantitatively measure Progesterone metabolites present in extracted fecal samples and urine. This assay provides another valuable tool for profiling progestrone metabolites. The cross-reactivity profile of the newest assay includes important biomarkers absent from previous kits. Arbor Assays Kit Comparison Progesterone EIA Kit, Progesterone Metabolites EIA Kit, K025-H1/H5 K068-H1/H5 Standard Range 50-3,200 pg/mL 102.4-10,000 pg/mL Sensitivity 47.9 pg/mL 51.2 pg/mL Samples/Kit 39 or 231 in Duplicate 40 or 232 in Duplicate (1 plate & 5 plate) Validated Sample Fecal, Urine, TCM Fecal, Urine Type Time to Answer 150 Minutes 90 Minutes Cross Reactivity Progesterone: 100% Progessterone: 100% Profile 3β-hydroxy-progesterone: 172% 5α-dihydroprogesterone: 56.7% 3α-hydroxy-progesterone: 188% 5β-dihydroprogesterone: 61.9% 11α-hydroxy-progesterone: 147% Epiallopregnanolone: 38.3% 11β-hydroxy-progesterone: 2.7% Pregnanolone: 41.2% 5α-dihydroprogesterone: 7.0% Allopregnandione: 27.3% Pregnenolone: 5.9% Pregnenolone: 17.6% Corticosterone: <0.1% Epipregnanolone: 10.2% Androstenedione: <0.1% Allopregnandiol: 0.29% 11α-hydroxyprogesterone: 4.9% 17α-hydroxyprogesterone: 5.7% 20α-hydroxyprogesterone: 0.34% WWW.ARBORASSAYS.COM.

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Effect of Isopregnanolone on Rapid Tolerance to the Anxiolytic Effect of Ethanol Influência Da Isopregnenolona Na Tolerância R
18 ORIGINAL ARTICLE Effect of isopregnanolone on rapid tolerance to the anxiolytic effect of ethanol Influência da isopregnenolona na tolerância rápida ao efeito ansiolítico do etanol Thaize Debatin,1 Adriana Dias Elpo Barbosa2 Original version accepted in Portuguese Abstract Objective: It has been shown that neurosteroids can either block or stimulate the development of chronic and rapid tolerance to the incoordination and hypothermia caused by ethanol consumption. The aim of the present study was to investigate the influence of isopregnanolone on the development of rapid tolerance to the anxiolytic effect of ethanol in mice. Method: Male Swiss mice were pretreated with isopregnanolone (0.05, 0.10 or 0.20 mg/kg) 30 min before administration of ethanol (1.5 g/kg). Twenty-four hours later, all animals we tested using the plus-maze apparatus. The first experiment defined the doses of ethanol that did or did not induce rapid tolerance to the anxiolytic effect of ethanol. In the second, the influence of pretreatment of mice with isopregnanolone (0.05, 0.10 or 0.20 mg/kg) on rapid tolerance to ethanol (1.5 g/kg) was studied. Conclusions: The results show that pretreatment with isopregnanolone interfered with the development of rapid tolerance to the anxiolytic effect of ethanol. Keywords: Ethanol; Drug tolerance; Anti-anxiety agents; Mice; Alcoholism Resumo Objetivo: Estudos prévios têm mostrado que os neuroesteróides podem bloquear ou estimular o desenvolvimento da tolerância rápida e crônica aos efeitos de incoordenação e hipotermia produzidos pelo etanol. O objetivo do presente estudo foi investigar a influência da isopregnenolona sobre o desenvolvimento da tolerância rápida ao efeito ansiolítico do etanol em camundongos.
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Epipregnanolone and a Novel Synthetic Neuroactive Steroid Reduce Reduce Alcohol Self- Administration in Rats
University of Texas at El Paso From the SelectedWorks of Laura Elena O'Dell 2005 Epipregnanolone and a novel synthetic neuroactive steroid reduce reduce alcohol self- administration in rats. Laura O'Dell, University of Texas at El Paso Available at: https://works.bepress.com/laura_odell/23/ Pharmacology, Biochemistry and Behavior 81 (2005) 543 – 550 www.elsevier.com/locate/pharmbiochembeh Epipregnanolone and a novel synthetic neuroactive steroid reduce alcohol self-administration in rats L.E. O’Della,b, R.H. Purdya,c,d, D.F. Coveye, H.N. Richardsona, M. Robertoa, G.F. Kooba,* aDepartment of Neuropharmacology, The Scripps Research Institute, CVN-7, 10550 North Torrey Pines Rd., La Jolla, CA, 92037, USA bDepartment of Psychology, The University of Texas at El Paso, El Paso, TX, USA cDepartment of Psychiatry, University of California San Diego, La Jolla, CA, USA dDepartment of Veterans Affairs Medical Center and Veterans Medical Research Foundation, San Diego, CA, USA eDepartment of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO, USA Received 6 September 2004; received in revised form 14 March 2005; accepted 31 March 2005 Available online 9 June 2005 Abstract This study was designed to compare the effects of several neuroactive steroids with varying patterns of modulation of g-aminobutyric acid (GABA)A and NMDA receptors on operant self-administration of ethanol or water. Once stable responding for 10% (w/v) ethanol was achieved, separate test sessions were conducted in which male Wistar rats were allowed to self-administer ethanol or water following pre-treatment with vehicle or one of the following neuroactive steroids: (3h,5h)-3-hydroxypregnan-20-one (epipregnanolone; 5, 10, 20 mg/kg; n =12), (3a,5h)-20-oxo-pregnane-3-carboxylic acid (PCA; 10, 20, 30 mg/kg; n =10), (3a,5h)-3-hydroxypregnan-20-one hemisuccinate (pregnanolone hemisuccinate; 5, 10, 20 mg/kg; n =12) and (3a,5a)-3-hydroxyandrostan-17-one hemisuccinate (androsterone hemisuccinate; 5, 10, 20 mg/kg; n =11).
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Download Product Insert (PDF)
PRODUCT INFORMATION Epipregnanolone Item No. 34295 CAS Registry No.: 128-21-2 O Formal Name: (5β)-3β-hydroxy-pregnan-20-one Synonyms: NSC 21450, 5β-Pregnan-3β-ol-20-one MF: C21H34O2 FW: 318.5 H Purity: ≥98% H H Supplied as: A solid Storage: -20°C HO Stability: ≥2 years H Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis. Laboratory Procedures Epipregnanolone is supplied as a solid. A stock solution may be made by dissolving the epipregnanolone in the solvent of choice, which should be purged with an inert gas. Epipregnanolone is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of epipregnanolone in ethanol is approximately 5 mg/ml and approximately 30 mg/ml in DMSO and DMF. Description Epipregnanolone is a neurosteroid and an active metabolite of the steroid hormone pregnenolone (Item No. 19864).1 It is enzymatically formed from prognenolone via the intermediates progesterone (Item No. 15876) and 5β-dihydroprogesterone in the placenta.2 Epipregnanolone inhibits spontaneous 3 contractions in myometrial strips isolated from at-term pregnant women (IC50 = 156 µM). Epipregnanolone (10 and 20 mg/kg) decreases operant alcohol self-administration in rats.4 Maternal plasma levels of epipregnanolone increase over the duration of pregnancy. References 1. Prince, R.J. and Simmonds, M.A. 5β-pregnan-3β-ol-20-one, a specific antagonist at the neurosteroid site of the GABAA receptor-complex. Neurosci. Lett. 135(2), 273-275 (1992). 2. Hill, M., Cibula, D., Havlíkova, H., et al. Circulating levels of pregnanolone isomers during the third trimester of human pregnancy.
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Alteration of the Steroidogenesis in Boys with Autism Spectrum Disorders
Janšáková et al. Translational Psychiatry (2020) 10:340 https://doi.org/10.1038/s41398-020-01017-8 Translational Psychiatry ARTICLE Open Access Alteration of the steroidogenesis in boys with autism spectrum disorders Katarína Janšáková 1, Martin Hill 2,DianaČelárová1,HanaCelušáková1,GabrielaRepiská1,MarieBičíková2, Ludmila Máčová2 and Daniela Ostatníková1 Abstract The etiology of autism spectrum disorders (ASD) remains unknown, but associations between prenatal hormonal changes and ASD risk were found. The consequences of these changes on the steroidogenesis during a postnatal development are not yet well known. The aim of this study was to analyze the steroid metabolic pathway in prepubertal ASD and neurotypical boys. Plasma samples were collected from 62 prepubertal ASD boys and 24 age and sex-matched controls (CTRL). Eighty-two biomarkers of steroidogenesis were detected using gas-chromatography tandem-mass spectrometry. We observed changes across the whole alternative backdoor pathway of androgens synthesis toward lower level in ASD group. Our data indicate suppressed production of pregnenolone sulfate at augmented activities of CYP17A1 and SULT2A1 and reduced HSD3B2 activity in ASD group which is partly consistent with the results reported in older children, in whom the adrenal zona reticularis significantly influences the steroid levels. Furthermore, we detected the suppressed activity of CYP7B1 enzyme readily metabolizing the precursors of sex hormones on one hand but increased anti-glucocorticoid effect of 7α-hydroxy-DHEA via competition with cortisone for HSD11B1 on the other. The multivariate model found significant correlations between behavioral indices and circulating steroids. From dependent variables, the best correlation was found for the social interaction (28.5%). Observed changes give a space for their utilization as biomarkers while reveal the etiopathogenesis of ASD.
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Calcium-Engaged Mechanisms of Nongenomic Action of Neurosteroids
Calcium-engaged Mechanisms of Nongenomic Action of Neurosteroids The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Rebas, Elzbieta, Tomasz Radzik, Tomasz Boczek, and Ludmila Zylinska. 2017. “Calcium-engaged Mechanisms of Nongenomic Action of Neurosteroids.” Current Neuropharmacology 15 (8): 1174-1191. doi:10.2174/1570159X15666170329091935. http:// dx.doi.org/10.2174/1570159X15666170329091935. Published Version doi:10.2174/1570159X15666170329091935 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:37160234 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA 1174 Send Orders for Reprints to [email protected] Current Neuropharmacology, 2017, 15, 1174-1191 REVIEW ARTICLE ISSN: 1570-159X eISSN: 1875-6190 Impact Factor: 3.365 Calcium-engaged Mechanisms of Nongenomic Action of Neurosteroids BENTHAM SCIENCE Elzbieta Rebas1, Tomasz Radzik1, Tomasz Boczek1,2 and Ludmila Zylinska1,* 1Department of Molecular Neurochemistry, Faculty of Health Sciences, Medical University of Lodz, Poland; 2Boston Children’s Hospital and Harvard Medical School, Boston, USA Abstract: Background: Neurosteroids form the unique group because of their dual mechanism of action. Classically, they bind to specific intracellular and/or nuclear receptors, and next modify genes transcription. Another mode of action is linked with the rapid effects induced at the plasma membrane level within seconds or milliseconds. The key molecules in neurotransmission are calcium ions, thereby we focus on the recent advances in understanding of complex signaling crosstalk between action of neurosteroids and calcium-engaged events.
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Comparison of Strategies for the Determination of Sterol Sulfates Via GC-MS Leading to a Novel Deconjugation-Derivatization Protocol
molecules Article Comparison of Strategies for the Determination of Sterol Sulfates via GC-MS Leading to a Novel Deconjugation-Derivatization Protocol Julia Junker 1 , Isabelle Chong 1, Frits Kamp 2, Harald Steiner 2,3, Martin Giera 4 , Christoph Müller 1 and Franz Bracher 1,* 1 Department of Pharmacy-Center for Drug Research, Ludwig-Maximilians University Munich, Butenandtstraße 5-13, 81377 Munich, Germany 2 Biomedical Center (BMC), Metabolic Biochemistry, Ludwig-Maximilians University Munich, Feodor-Lynen-Strasse 17, 81377 Munich, Germany 3 German Center for Neurodegenerative Diseases (DZNE), Feodor-Lynen-Strasse 17, 81377 Munich, Germany 4 Leiden University Medical Center, Center for Proteomics and Metabolomics, Albinusdreef 2, 2300 RC Leiden, The Netherlands * Correspondence: [email protected]; Tel.: +49-892-1807-7301 Academic Editor: Yasunori Yaoita Received: 27 April 2019; Accepted: 21 June 2019; Published: 26 June 2019 Abstract: Sulfoconjugates of sterols play important roles as neurosteroids, neurotransmitters, and ion channel ligands in health and disease. In most cases, sterol conjugate analysis is performed with liquid chromatography-mass spectrometry. This is a valuable tool for routine analytics with the advantage of direct sterol sulfates analysis without previous cleavage and/or derivatization. The complementary technique gas chromatography-mass spectrometry (GC-MS) is a preeminent discovery tool in the field of sterolomics, but the analysis of sterol sulfates is hampered by mandatory deconjugation and derivatization. Despite the difficulties in sample workup, GC-MS is an indispensable tool for untargeted analysis and steroid profiling. There are no general sample preparation protocols for sterol sulfate analysis using GC-MS. In this study we present a reinvestigation and evaluation of different deconjugation and derivatization procedures with a set of representative sterol sulfates.
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Fluoxetine Elevates Allopregnanolone in Female Rat Brain but Inhibits A
British Journal of DOI:10.1111/bph.12891 www.brjpharmacol.org BJP Pharmacology RESEARCH PAPER Correspondence Jonathan P Fry, Department of Neuroscience, Physiology and Pharmacology, University College Fluoxetine elevates London, Gower Street, London WC1E 6BT, UK. E-mail: [email protected] allopregnanolone in female ---------------------------------------------------------------- Received 27 March 2014 rat brain but inhibits a Revised 3 July 2014 Accepted steroid microsomal 18 August 2014 dehydrogenase rather than activating an aldo-keto reductase JPFry1,KYLi1, A J Devall2, S Cockcroft1, J W Honour3,4 and T A Lovick5 1Department of Neuroscience, Physiology and Pharmacology, 4Institute of Women’s Health, University College London (UCL), 3Department of Chemical Pathology, University College London Hospital, London, 2School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, and 5School of Physiology and Pharmacology, University of Bristol, Bristol, UK BACKGROUND AND PURPOSE Fluoxetine, a selective serotonin reuptake inhibitor, elevates brain concentrations of the neuroactive progesterone metabolite allopregnanolone, an effect suggested to underlie its use in the treatment of premenstrual dysphoria. One report showed fluoxetine to activate the aldo-keto reductase (AKR) component of 3α-hydroxysteroid dehydrogenase (3α-HSD), which catalyses production of allopregnanolone from 5α-dihydroprogesterone. However, this action was not observed by others. The present study sought to clarify the site of action for fluoxetine in elevating brain allopregnanolone. EXPERIMENTAL APPROACH Adult male rats and female rats in dioestrus were treated with fluoxetine and their brains assayed for allopregnanolone and its precursors, progesterone and 5α-dihydroprogesterone. Subcellular fractions of rat brain were also used to investigate the actions of fluoxetine on 3α-HSD activity in both the reductive direction, producing allopregnanolone from 5α-dihydroprogesterone, and the reverse oxidative direction.
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Novel Targets for Neuroactive Steroid Synthesis and Action and Their Relevance for Translational Research P
Journal of Neuroendocrinology, 2016, 28, 10.1111/jne.12351 REVIEW ARTICLE © 2015 British Society for Neuroendocrinology Neurosteroidogenesis Today: Novel Targets for Neuroactive Steroid Synthesis and Action and Their Relevance for Translational Research P. Porcu*, A. M. Barron†, C. A. Frye‡§, A. A. Walf‡§¶, S.-Y. Yang**, X.-Y. He**, A. L. Morrow††, G. C. Panzica‡‡ and R. C. Melcangi§§ *Neuroscience Institute, National Research Council of Italy (CNR), Cagliari, Italy. †Molecular Imaging Center, National Institute of Radiological Sciences, Anagawa, Inage-ku, Chiba, Japan. ‡IDEA Network of Biomedical Research Excellence and Department of Chemistry and Biochemistry, University of Alaska–Fairbanks, Fairbanks, AK, USA. §Department of Psychology, The University at Albany, Albany, NY, USA. ¶Department of Cognitive Science, Rensselaer Polytechnic Institute, Troy, NY, USA. **Department of Developmental Biochemistry, NYS Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA. ††Departments of Psychiatry and Pharmacology, Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, NC, USA. ‡‡Department of Neuroscience, University of Turin, and NICO – Neuroscience Institute Cavalieri Ottolenghi, Orbassano, Italy. §§Dipartimento di Scienze Farmacologiche e Biomolecolari, Universita degli Studi di Milano, Milan, Italy. Journal of Neuroactive steroids are endogenous neuromodulators synthesised in the brain that rapidly alter Neuroendocrinology neuronal excitability by binding to membrane
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Reduced Progesterone Metabolites in Human Late Pregnancy
Physiol. Res. 60: 225-241, 2011 https://doi.org/10.33549/physiolres.932077 REVIEW Reduced Progesterone Metabolites in Human Late Pregnancy M. HILL1,2, A. PAŘÍZEK2, R. KANCHEVA1, J. E. JIRÁSEK3 1,2Institute of Endocrinology, Prague, Czech Republic, 2Department of Obstetrics and Gynecology of the First Faculty of Medicine and General Teaching Hospital, Prague, Czech Republic, 3Department of Clinical Biochemistry and Laboratory Diagnostics of the First Faculty of Medicine and General Teaching Hospital, Prague, Czech Republic Received November 20, 2010 Accepted November 25, 2010 On-line November 29, 2010 Summary Corresponding author In this review, we focused on the intersection between steroid A. Pařízek, Department of Obstetrics and Gynecology of the metabolomics, obstetrics and steroid neurophysiology to give a First Faculty of Medicine and General Teaching Hospital, comprehensive insight into the role of sex hormones and Apolinářská 18, 128 51 Prague 2, Czech Republic. E-mail: neuroactive steroids (NAS) in the mechanism controlling [email protected] pregnancy sustaining. The data in the literature including our studies show that there is a complex mechanism providing synthesis of either pregnancy sustaining or parturition provoking Introduction steroids. This mechanism includes the boosting placental synthesis of CRH with approaching parturition inducing the Although the effects of neuroactive and excessive synthesis of 3β-hydroxy-5-ene steroid sulfates serving neuroprotective 5α/β-reduced progesterone metabolites primarily as precursors for placental synthesis of progestogens, were extensively studied, the physiological relevance of estrogens and NAS. The distribution and changing activities of these substances remains frequently uncertain due to the placental oxidoreductases are responsible for the activation or lack of metabolomic data.
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Chronic Cigarette Smoking Alters Circulating Sex Hormones and Neuroactive Steroids in Premenopausal Women
Physiol. Res. 61: 97-111, 2012 https://doi.org/10.33549/physiolres.932164 Chronic Cigarette Smoking Alters Circulating Sex Hormones and Neuroactive Steroids in Premenopausal Women M. DUŠKOVÁ1, K. ŠIMŮNKOVÁ1, M. HILL1,4, M. VELÍKOVÁ1, J. KUBÁTOVÁ1, L. KANCHEVA1, H. KAZIHNITKOVÁ1, H. HRUŠKOVIČOVÁ1, H. POSPÍŠILOVÁ1, B. RÁCZ1, M. SALÁTOVÁ1, V. CIRMANOVÁ1, E. KRÁLÍKOVÁ2,3, L. STÁRKA1, A. PAŘÍZEK4 1Institute of Endocrinology, Prague, Czech Republic, 2Institute of Hygiene and Epidemiology, First Faculty of Medicine, Charles University, Prague, Czech Republic, 3Tobacco Dependence Treatment Centre, Third Medical Department – Department of Metabolism and Endocrinology, First Faculty of Medicine, Charles University, Prague, Czech Republic, 4Department of Obstetrics and Gynecology of the First Faculty of Medicine, Charles University, Prague, Czech Republic Received February 18, 2011 Accepted October 27, 2011 On-line December 20, 2011 Summary lutropin/follitropin ratio. In conclusion, chronic cigarette smoking Chronic smoking alters the circulating levels of sex hormones and augments serum androgens and their 5α/β-reduced metabolites possibly also the neuroactive steroids. However, the data (including GABAergic substances) but suppresses the levels of available is limited. Therefore, a broad spectrum of free and estradiol in the LP and SHBG and may induce hyperandrogenism conjugated steroids and related substances was quantified by in female smokers. The female smokers had pronouncedly GC-MS and RIA in premenopausal smokers and in age-matched increased serum progestogens but paradoxically suppressed (38.9±7.3 years of age) non-smokers in the follicular (FP) and levels of their GABA-ergic metabolites. Further investigation is luteal phases (LP) of menstrual cycle (10 non-smokers and needed concerning these effects.
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The Sigma1 Protein As a Target for the Non-Genomic Effects of Neuro(Active)Steroids: Molecular, Physiological, and Behavioral Aspects François P
J Pharmacol Sci 100, 93 – 118 (2006) Journal of Pharmacological Sciences ©2006 The Japanese Pharmacological Society Critical Review The Sigma1 Protein as a Target for the Non-genomic Effects of Neuro(active)steroids: Molecular, Physiological, and Behavioral Aspects François P. Monnet1 and Tangui Maurice2,* 1Unité 705 de l’Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 7157 du Centre National de la Recherche Scientifique, Université de Paris V et VII, Hôpital Lariboisière-Fernand Widal, 2, rue Ambroise Paré, 75475 Paris cedex 10, France 2Unité 710 de l’Institut National de la Santé et de la Recherche Médicale, Ecole Pratique des Hautes Etudes, Université de Montpellier II, cc 105, place Eugène Bataillon, 34095 Montpellier cedex 5, France Received December 15, 2005 Abstract. Steroids synthesized in the periphery or de novo in the brain, so called ‘neuro- steroids’, exert both genomic and nongenomic actions on neurotransmission systems. Through rapid modulatory effects on neurotransmitter receptors, they influence inhibitory and excitatory neurotransmission. In particular, progesterone derivatives like 3α-hydroxy-5α-pregnan-20-one (allopregnanolone) are positive allosteric modulators of the γ-aminobutyric acid type A (GABAA) receptor and therefore act as inhibitory steroids, while pregnenolone sulphate (PREGS) and dehydroepiandrosterone sulphate (DHEAS) are negative modulators of the GABAA receptor and positive modulators of the N-methyl-D-aspartate (NMDA) receptor, therefore acting as excitatory neurosteroids. Some steroids also interact with atypical proteins, the sigma (σ) receptors. Recent studies particularly demonstrated that the σ1 receptor contributes effectively to their pharmaco- logical actions. The present article will review the data demonstrating that the σ1 receptor binds neurosteroids in physiological conditions.
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Multidisciplinary Analysis of Biological Effects of Novel Analogs of The
UNIVERSITÉ DE STRASBOURG ÉCOLE DOCTORALE des Sciences de la vie et de la santé (ED 414) INSERM UMR_S U1119 Biopathologies de la Myéline, Neuroprotection et Stratégies Thérapeutiques THÈSE En cotutelle avec l´Albert-Ludwigs-Universität Freiburg, Allemagne présentée par : Mona KAROUT soutenue le : 30 Septembre 2015 pour obtenir le grade de : Docteur de l’université de Strasbourg Discipline / Spécialité : Neurosciences Multidisciplinary analysis of biological effects of novel analogs of the neurosteroid allopregnanolone: evidence for a proliferative, neurogenic and ne uroprote ctive action THÈSE dirigée par : M. MENSAH-NYAGAN Ayikoé, Guy Professeur, Université de Strasbourg, France M. HOFMANN Hans-Dieter Professeur, Albert-Ludwigs-Universität Freiburg, Allemagne RAPPORTEURS : M. FISCHBACH Karl-Friedrich Professeur, Albert-Ludwigs-Universität Freiburg, Allemagne M. SCHWEMMLE Martin Professeur, Albert-Ludwigs-Universität Freiburg, Allemagne AUTRES MEMBRES DU JURY : M. DUFOUR André Professeur, Université de Strasbourg, France Mme. PATTE-MENSAH Christine Docteur, Université de Strasbourg, France !"# $%&'(%# To my parents A mes parents Acknowledgement ACKNOWLEDGEMENT First of all, I would like to express my appreciation and gratitude to all members of my thesis committee, Prof. Karl-Friedrich Fischbach, Prof. Martin Schwemmle, Prof. André Dufour and Dr. Christine Patte-Mensah for taking the time to evaluate my thesis. Furthermore, I would like to express my deepest gratitude to my two PhD supervisors Prof. Ayikoé Guy Mensah-Nyagan and Prof. Hans-Dieter Hofmann. I am very thankful to you Prof. Mensah-Nyagan for giving me a great opportunity for performing this joint French-German PhD. Thank you very much for the continuous support, for your patience, enthusiasm, passion for research and immense knowledge.
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