Product Datasheet
NFKBIE Antibody
Catalog No: #35589
Orders: [email protected] Description Support: [email protected]
Product Name NFKBIE Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous levels of total NFKBIE protein.
Immunogen Type Recombinant Protein
Immunogen Description Fusion protein corresponding to a region derived from internal residues of human Nuclear factor of kappa light
polypeptide gene enhancer in B-cells inhibitor, epsilon
Target Name NFKBIE
Other Names IKBE
Accession No. Swiss-Prot#: O00221NCBI Gene ID: 4794Gene Accssion: BC011676
SDS-PAGE MW 45kd
Concentration 1.4mg/ml
Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Storage Store at -20°C
Application Details
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200
Images
Gel: 10%SDS-PAGE Lysate: 40ug Raji cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute
Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #35589 at dilution 1/40.
Background
The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. NFKBIE protein expression is up-regulated following NF-κB activation and during myelopoiesis. NFKBIE is able to inhibit NF-κB-directed transactivation via cytoplasmic retention of REL proteins. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-κB transcription factor complex. The NF-κB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm.
Note: This product is for in vitro research use only and is not intended for use in humans or animals.
Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2