PR01C Treatment Of Osteomyelitis In New Zealand White Rabbit Using Impregnated Biomaterials With Gentamicin: In Vivo Study Outcome Of Bacterial Study And Micro-Computed Topography Evaluation 1Zulkifly AH, 1Mohd Jan NH, 1Ibrahim MZ, 2Aziz A 1Department of Orthopaedics, Traumatology and Rehabilitation, , International Islamic University , Jalan Hospital Campus, , Darul Makmur, 25150, Malaysia. 2Department of Orthopaedics, Hospital Tengku Ampuan Afzan, Kuantan, Pahang Darul Makmur, 25150, Malaysia

INTRODUCTION: Table 1 showing the bone analysis through Treatment of osteomyelitis is still a major Skyscan1176 system. Trabecul challenge in orthopaedic field. New Zealand Tissue Bone Bone Week & ar White rabbit is acceptable experimental model volume volume surface parameter thickness described to develop effective local delivery of (mm3) (mm3) (mm3) (mm) antibiotics osteomyelitis treatment as it can 3 W 399. 156.692 66.962 0.045 mimics the disease process in human. The SHAM 351 3W post 500. objective of the study is to evaluate the 168.051 84.762 0.405 outcome treatment of osteomyelitis in rabbit treatment 962 6W post 1001. femur with impregnated Hydroxyappatite 409.204 104.952 0.456 treatment 135 Gentamicin. Evaluation at stipulated durations 12 W 1520. 590.716 113.485 0.549 using microCT Skyscan 1176 system. treatment 599 26w post 1558. 635.955 124.958 0.723 MATERIALS & METHODS: treatment 096 Osteomyelitis is created by inoculation of Staphylococcus aureus ATCC 25923 into DISCUSSIONS: rabbit’s distal femur. The rabbit were divided Osteomyelitic changes were noted in all rabbit with 3, 6, 12, 26 week assessments. The after inoculation of bacteria at week three and response of the biomaterials (hydroxyapatite) six. The micro-computed topographic analysis impregnated with gentamicin against the at 3, 6, 12 and 26 weeks of interval, showed infection were evaluated through micro- increase bone parameters in treated femur. The computed topography using Skyscan 1176 bone volume (BV) was increased with value 3 system.The bone analysis data were obtained 124.96 mm compared to sham group (no 3 by the software provided by Skyscan 1176 treatment given) with bone volume 66.96 mm . system. The distal femoral shaft were scanned The density of biomaterials was decreased with using A1 1mm filter with 18 µm pixel duration of studies. This data showed that resolutions. Bacterial study of culture and micro-computed topography studies is capable sensitivity, coagulase test, catalase test and to provide information of bone microstructures gram stain were done to determine the in rapid and non-destructive way. The findings treatment is effective. showed that impregnated biomaterials with gentamicin have potential to be used in RESULTS: osteomyelitis infection. The data of bone analysis were for tissue volume (TV), bone surface (BS), bone volume CONCLUSION: (BV), and trabecular thickness (Tb.Th) in all Computed topography results showed at 26 groups were illustrated at Table 1. The bone weeks of treatment, the impregnated healing noted in the treated group. The results biomaterials have a potential in treatment of showed no bacterial were grow in the agar and osteomyelitis. all test were negative at 26 weeks interval.